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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB863
Library VH
(Link to library)
Clone ID VHB863
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15687-1
Original site URL
Representative seq. ID VHB863P
(Link to Original site)
Representative DNA sequence 
>VHB863 (VHB863Q) /CSM/VH/VHB8-C/VHB863Q.Seq.d/
GAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTCAAAATGAAGA
ATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGCAACAATTAAC
TAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAGAAGAAGCATT
AGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAATTTATAATAA
ACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAATTTTCAATAA
AGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCAGA
AAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAATCATCAGAAGG
TGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTATCAGCATTTGT
AAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGATCTATCAATC
ACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTGTCTATTATTC
AATGATTGATATAXXXXXXXXXXAGAATATCAAATACTTTAGAGAGAATAAAGAACAATT
GAATAATATGTACCNTTCAGAGTTGGTGACAGAGTTGGAAAGAGACATTAGACAATTGGG
TGTCTCCGAGACTGGTTTAACCTTTTTAGATCATTTTAGACTTTTAATCACTCATATTGG
TAATGCTATGGGTTATATTAGATTGGTCAGATCCGGTGGTTTACACTATTGTAGTAATGC
TATCAAATTCGTACCAGACTTGAAAAAGATTCCAAAATTCCAAGATTTAACCTCAAAAGA
TGCCCTCTCACCTGAAACAATTCAAGCTAGTACCAATTTGGATTCAATCATTCATAATCT
TTCAAATAATTTATCAGAGGGTACTGAATACTTTAAGATGTTGGTAAACGTTTTCGCTAC
AGAGTTTCGAAATATCGCTAATCAACATTTAAAGAATTTCTATATCATCGTACCTGCCTT
GGCCTCAAATTACATCGATCATATGATCAATAGTAAAGATAAACTCTTTAAGAAATCAAA
AGCAATCGGTGCTGAAGCTTTATTATTCACTGATGATGGCTTTGCAATTGGTTTGGCTTA
TATCTTAAAATTATTAGATCAAAATAAAGATTTCGATTCTCTTCATTGGTTCGATCGTAT
TCAATTGAAATGTGAAACTGATCAAAAGAGAATGTTGGCTGAAGCAAATGCAAAAGGTAT
CAAAGAGGATCAAAATCATTTAGCAATTAAAAAGATTCAAAATTTCCAACAAGAATAT
sequence update 2002.10.25
Translated Amino Acid sequence 
nnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIEEAL
DETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILKETAE
SKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASIYQS
QKNIHTQFFKNVHLQAVYYSMIDI---

---NIKYFRENKEQLNNMYXSELVTELERDIRQLGVSETGLTFLDHFRLLITHIGNAMGY
IRLVRSGGLHYCSNAIKFVPDLKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNLS
EGTEYFKMLVNVFATEFRNIANQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGAE
ALLFTDDGFAIGLAYILKLLDQNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKEDQN
HLAIKKIQNFQQEY
Translated Amino Acid sequence (All Frames) 
Frame A:
eqpnlenghilfg**wsk*riyi*ci*rwfi*igwratin*t*sics*lcttnledrrsi
r*nik*smgykyrssfnl**ts*tirfn*ig*n*s*nfq*snisilfnl*sn*nfkrnsr
k*ilftinsiwrdyr*iirr*ctn*sw*iitiyd*fisick*mlfin*kyy*sicidlsi
tkeytytil*ecsftscllfnd*y---

---risntleriknn*iictxqsw*qswketldnwvsprlv*pf*iildf*slilvmlwv
ildwsdpvvytivvmlsnsyqt*krfqnski*pqkmpshlkqfklvpiwiqsfiifqiiy
qrvlntlrcw*tfslqsfeislini*risissylpwpqitsii*sivkinslrnqkqsvl
klyyslmmalqlvwlis*ny*ikikisilfigsivfn*nvklikrecwlkqmqkvskrik
ii*qlkrfkisnkn

Frame B:
nnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIEEAL
DETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILKETAE
SKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASIYQS
QKNIHTQFFKNVHLQAVYYSMIDI---

---eyqil*re*rtie*yvpfrvgdrvgkrh*tigclrdwfnlfrsf*tfnhsyw*cygl
y*igqirwftll**cyqirtrlekdskiprfnlkrcplt*nnss*yqfgfnhs*sfk*fi
rgy*il*dvgkrfryrvskyr*stfkeflyhrtclglklhrsydq**r*tl*eiksnrc*
sfiih**wlcnwfglylkiirsk*rfrfsslvrsysiem*n*skenvg*skckryqrgsk
sfsn*kdskfptri

Frame C:
ttqfrkwphpirlimvkmknihlmhlkmvhlnwlesnn*lnlinlqlimhnkfgr*kkh*
mkh*mkygi*i*iqfqfiinlmnn*i*lnwlkliikfsik*y*yslqfviklef*kkqqk
vnfihh*qylerlqvnhqkvmyklklvnyyhl*liyqhl*idvih*leillvnlhrsinh
kriyihnslrmfiyklsiiq*li---

---NIKYFRENKEQLNNMYXSELVTELERDIRQLGVSETGLTFLDHFRLLITHIGNAMGY
IRLVRSGGLHYCSNAIKFVPDLKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNLS
EGTEYFKMLVNVFATEFRNIANQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGAE
ALLFTDDGFAIGLAYILKLLDQNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKEDQN
HLAIKKIQNFQQEY
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB863 (VHB863Q) /CSM/VH/VHB8-C/VHB863Q.Seq.d/                       2666   0.0
VHK424 (VHK424Q) /CSM/VH/VHK4-A/VHK424Q.Seq.d/                       2656   0.0
VHK538 (VHK538Q) /CSM/VH/VHK5-B/VHK538Q.Seq.d/                       1491   0.0
SHD671 (SHD671Q) /CSM/SH/SHD6-C/SHD671Q.Seq.d/                       1491   0.0
AHL641 (AHL641Q) /CSM/AH/AHL6-B/AHL641Q.Seq.d/                       1491   0.0
VHA138 (VHA138Q) /CSM/VH/VHA1-B/VHA138Q.Seq.d/                       1489   0.0
CHR573 (CHR573Q) /CSM/CH/CHR5-D/CHR573Q.Seq.d/                       1489   0.0
VHH719 (VHH719Q) /CSM/VH/VHH7-A/VHH719Q.Seq.d/                       1483   0.0
VHB619 (VHB619Q) /CSM/VH/VHB6-A/VHB619Q.Seq.d/                       1483   0.0
CHG465 (CHG465Q) /CSM/CH/CHG4-C/CHG465Q.Seq.d/                       1483   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BU746157|BU746157.1 CH3#002_C07T7 Canine heart normalized cDNA Library in pBluescript Canis familiaris cDNA clone CH3#002_C07 5', mRNA sequence.    38   0.001  3
CP000263|CP000263.1 Buchnera aphidicola str. Cc (Cinara cedri), complete genome.    36   0.013  15
BY464171|BY464171.1 Mus musculus 15 days pregnant adult female amnion cDNA, RIKEN full-length enriched library, clone:K630150M24, 3' end partial sequence.    42   0.035  2
AK113047|AK113047.1 Ciona intestinalis cDNA, clone:ciad002a21, full insert sequence.    48   0.040  2
BB822673|BB822673.1 Mus musculus mammary gland RCB-0526 Jyg-MC(A) cDNA, RIKEN full-length enriched library, clone:G830022O04, 3' end partial sequence.    32   0.14   4
DN287505|DN287505.1 1241755 MARC 7BOV Bos taurus cDNA 3', mRNA sequence.    34   0.16   3
CR103667|CR103667.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP142o16.    50   0.17   1
CR044130|CR044130.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP186g09.    50   0.17   1
CR034434|CR034434.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP47e19.    50   0.17   1
CR023465|CR023465.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP421e10.    50   0.17   1
dna update 2007. 4.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54R74) RecName: Full=UPF0681 protein;                               501   e-140
BC040936_1(BC040936|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   248   4e-64
AB028956_1(AB028956|pid:none) Homo sapiens mRNA for KIAA1033 pro...   248   5e-64
BC143373_1(BC143373|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   248   5e-64
BC020314_1(BC020314|pid:none) Mus musculus RIKEN cDNA A230046K03...   246   2e-63
(Q3UMB9) RecName: Full=UPF0681 protein KIAA1033;                      246   2e-63
AK145000_1(AK145000|pid:none) Mus musculus mammary gland RCB-052...   246   2e-63
AK161247_1(AK161247|pid:none) Mus musculus adult male testis cDN...   245   3e-63
BC110850_1(BC110850|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   191   4e-47
AX882617_1(AX882617|pid:none) Sequence 17522 from Patent EP10746...   187   1e-45
protein update 2009. 6.27
PSORT 

 psg: 0.79  gvh: 0.25  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VHB863 is nuc
5' end seq. ID VHB863F
5' end seq. 
>VHB863F.Seq
GAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTCAAAATGAAGA
ATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGCAACAATTAAC
TAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAGAAGAAGCATT
AGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAATTTATAATAA
ACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAATTTTCAATAA
AGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCAGA
AAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAATCATCAGAAGG
TGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTATCAGCATTTGT
AAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGATCTATCAATC
ACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTGTCTATTATTC
AATGATTGATATANNNNNNNNNN
Length of 5' end seq. 623
3' end seq. ID VHB863Z
3' end seq. 
>VHB863Z.Seq
NNNNNNNNNNAGAATATCAAATACTTTAGAGAGAATAAAGAACAATTGAATAATATGTAC
CNTTCAGAGTTGGTGACAGAGTTGGAAAGAGACATTAGACAATTGGGTGTCTCCGAGACT
GGTTTAACCTTTTTAGATCATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGGT
TATATTAGATTGGTCAGATCCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGTA
CCAGACTTGAAAAAGATTCCAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCT
GAAACAATTCAAGCTAGTACCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTA
TCAGAGGGTACTGAATACTTTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAAT
ATCGCTAATCAACATTTAAAGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTAC
ATCGATCATATGATCAATAGTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCT
GAAGCTTTATTATTCACTGATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTA
TTAGATCAAAATAAAGATTTCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGT
GAAACTGATCAAAAGAGAATGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAA
AATCATTTAGCAATTAAAAAGATTCAAAATTTCCAACAAGAATAT
Length of 3' end seq. 765
Connected seq. ID VHB863P
Connected seq. 
>VHB863P.Seq
GAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTCAAAATGAAGA
ATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGCAACAATTAAC
TAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAGAAGAAGCATT
AGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAATTTATAATAA
ACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAATTTTCAATAA
AGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCAGA
AAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAATCATCAGAAGG
TGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTATCAGCATTTGT
AAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGATCTATCAATC
ACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTGTCTATTATTC
AATGATTGATATA----------AGAATATCAAATACTTTAGAGAGAATAAAGAACAATT
GAATAATATGTACCNTTCAGAGTTGGTGACAGAGTTGGAAAGAGACATTAGACAATTGGG
TGTCTCCGAGACTGGTTTAACCTTTTTAGATCATTTTAGACTTTTAATCACTCATATTGG
TAATGCTATGGGTTATATTAGATTGGTCAGATCCGGTGGTTTACACTATTGTAGTAATGC
TATCAAATTCGTACCAGACTTGAAAAAGATTCCAAAATTCCAAGATTTAACCTCAAAAGA
TGCCCTCTCACCTGAAACAATTCAAGCTAGTACCAATTTGGATTCAATCATTCATAATCT
TTCAAATAATTTATCAGAGGGTACTGAATACTTTAAGATGTTGGTAAACGTTTTCGCTAC
AGAGTTTCGAAATATCGCTAATCAACATTTAAAGAATTTCTATATCATCGTACCTGCCTT
GGCCTCAAATTACATCGATCATATGATCAATAGTAAAGATAAACTCTTTAAGAAATCAAA
AGCAATCGGTGCTGAAGCTTTATTATTCACTGATGATGGCTTTGCAATTGGTTTGGCTTA
TATCTTAAAATTATTAGATCAAAATAAAGATTTCGATTCTCTTCATTGGTTCGATCGTAT
TCAATTGAAATGTGAAACTGATCAAAAGAGAATGTTGGCTGAAGCAAATGCAAAAGGTAT
CAAAGAGGATCAAAATCATTTAGCAATTAAAAAGATTCAAAATTTCCAACAAGAATAT
Length of connected seq. 1368
Full length Seq ID -
Full length Seq. -
Length of full length seq. -