>VHB862 (VHB862Q) /CSM/VH/VHB8-C/VHB862Q.Seq.d/
ATTCAATTGTGTGATTGTGTTGTGTGTGTATTTTGGTTGATACACATTATATATATATAT
AATACAATAAAATGTCATTTTTTTTAGGACAGATTTTAGAAAAGATGGGTTCCATCGATA
AAGATATTAGATTCATGGCAACTCATGATCTTGCAAATGAATTAGAAAAAGATACTTTTA
AAATGGATCCAACTTATGAAAATAAAATTGTTACAAAATTATTAGCATTAACAGCTGATT
CAGCAAATAATGTTCAAGAAAATGTTGTTAAATGTTTAGGTTTATTAATTAAAAGAGTTA
AAGATTCACAAGCAACAGAAATTATTGATACATTATCAAAAAATATTTTGGAAGAATCAA
ATAAAGAAGAATTAGTTGAAATTTCAGGTATTGGTTTAAAAACAATCATTACAAATTTAC
CAAGTGAAGGTAGTTCAATTTCAACTTTAGTAATTAAAAATTTAGTACCAAAATTATTAA
TTGGTATTGATAGTGAAAAATTAAAAGATAAAAATGAAATTAAAATGTCATGTTTAGATA
TTTTAAATGATTTATTACAAAAGTATGGATCATTTATGATTGGAGATTTAGAGAATAXXX
XXXXXXXCAAATCACCATCACCATTGGAACGTTCAACCATTGGTAACCTCAATTAAATTC
TCAATCATGGAGAATAAAGAAGTTGTCGATCAATATTTAGCTCCAAACATCTCTCAATTC
CTTTCACTCTTACATGATGGTGATTTAATTGTTAGACGTAGTGCCCTCCTTTCATTGAAT
TACATTGCTCACAATAAACCAAATCTCATTCGTAATGATCTCTCTGTCTATTTACCAATC
TTGTACAACAATGCTAAAATCAAACCAGAACTCATTCGTGAAGTTGATCTTGGTCCATTC
AAACATAAGGTCGATGATGGTATTGAAATCAGAAAAACAGCTTTCGAATGTATGTACACT
CTTTTGGATACCTCTATCGATAAGATCGATGTAGCTCCATTCATCGTTTCATTATGTGAT
GGTCTCAAAGATACCCAATACGATATCAAATTGTTATGTCATTTAATGATCATTCGTTTA
GCAAACTCAAATGGTGCTGCTCTCCTCGAAAATATTACTCTCCTCCTTGAACCACTTCGT
GTTATTCTCATGACTAAAGTAAATGAAACCGCTGTTAAACAACAAATCGAACGTAATGAA
GAATGTATTCGTTCAGCTTTACGTGCTGTCGCTTCCATCTCTAGAATTCCAAATAGTGAT
AGTATCGTTAAATTTGAAGAATTTGTTAAAAATACAATTCGTACAACTCCATTAGCTGCT
CAATTTAATTCAATTTTATCGAAGATACAATGTCAAATCAAGATTCA

siv*lccvcilvdthyiyi*YNKMSFFLGQILEKMGSIDKDIRFMATHDLANELEKDTFK
MDPTYENKIVTKLLALTADSANNVQENVVKCLGLLIKRVKDSQATEIIDTLSKNILEESN
KEELVEISGIGLKTIITNLPSEGSSISTLVIKNLVPKLLIGIDSEKLKDKNEIKMSCLDI
LNDLLQKYGSFMIGDLEN---

---NHHHHWNVQPLVTSIKFSIMENKEVVDQYLAPNISQFLSLLHDGDLIVRRSALLSLN
YIAHNKPNLIRNDLSVYLPILYNNAKIKPELIREVDLGPFKHKVDDGIEIRKTAFECMYT
LLDTSIDKIDVAPFIVSLCDGLKDTQYDIKLLCHLMIIRLANSNGAALLENITLLLEPLR
VILMTKVNETAVKQQIERNEECIRSALRAVASISRIPNSDSIVKFEEFVKNTIRTTPLAA
QFNSILSKIQCQIKI

Frame A:
iqlcdcvvcvfwlihiiyiyntikchff*drf*krwvpsikildswqlmilqmn*kkill
kwiqlmkikllqny*h*qliqqimfkkmllnv*vy*lkelkihkqqkllihyqkifwknq
ikkn*lkfqvlv*kqslqiyqvkvvqfql**lki*yqny*lvlivkn*kikmklkchv*i
f*miyyksmdhl*lei*ri---

---qititigtfnhw*pqlnsqswrikklsini*lqtslnsfhsymmvi*lldvvpsfh*
itlltinqisfvmislsiyqscttmlksnqnsfvklilvhsnirsmmvlksekqlsnvct
lfwiplsirsm*lhssfhyvmvskipntisncyvi**sfv*qtqmvllsskillsslnhf
vlfs*lk*mkpllnnksnvmknvfvqlyvlslpslefqivivslnlknllkiqfvqlh*l
lnliqfyrrynvksrf

Frame B:
fncvivlcvyfg*ytlyiyiiq*nviffrtdfrkdgfhr*ry*ihgns*sck*irkryf*
ngsnl*k*ncykiisins*fsk*csrkcc*mfrfin*ks*rftsnrny*yiikkyfgrik
*rris*nfrywfknnhykftk*r*fnfnfsn*kfstkiinwy***kikr*k*n*nvmfry
fk*fitkvwiiydwrfre---

---kspsplerstignln*ilnhge*rscrsifsskhlsipftlt*w*fnc*t*cppfie
lhcsq*tkshs**slclftnlvqqc*nqtrths*s*swsiqt*gr*wy*nqknsfrmyvh
sfgylyr*drcssihrfim*wsqrypiryqivmsfndhsfsklkwccsprkyyspp*tts
cyshd*sk*nrc*ttnrt**rmysfsftccrfhl*nsk***yr*i*ric*kynsynsisc
si*fnfiedtmsnqds

Frame C:
siv*lccvcilvdthyiyi*YNKMSFFLGQILEKMGSIDKDIRFMATHDLANELEKDTFK
MDPTYENKIVTKLLALTADSANNVQENVVKCLGLLIKRVKDSQATEIIDTLSKNILEESN
KEELVEISGIGLKTIITNLPSEGSSISTLVIKNLVPKLLIGIDSEKLKDKNEIKMSCLDI
LNDLLQKYGSFMIGDLEN---

---NHHHHWNVQPLVTSIKFSIMENKEVVDQYLAPNISQFLSLLHDGDLIVRRSALLSLN
YIAHNKPNLIRNDLSVYLPILYNNAKIKPELIREVDLGPFKHKVDDGIEIRKTAFECMYT
LLDTSIDKIDVAPFIVSLCDGLKDTQYDIKLLCHLMIIRLANSNGAALLENITLLLEPLR
VILMTKVNETAVKQQIERNEECIRSALRAVASISRIPNSDSIVKFEEFVKNTIRTTPLAA
QFNSILSKIQCQIKI

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB862 (VHB862Q) /CSM/VH/VHB8-C/VHB862Q.Seq.d/                       1608   0.0
AHH811 (AHH811Q) /CSM/AH/AHH8-A/AHH811Q.Seq.d/                       1485   0.0
VHO635 (VHO635Q) /CSM/VH/VHO6-B/VHO635Q.Seq.d/                       1483   0.0
CHM192 (CHM192Q) /CSM/CH/CHM1-D/CHM192Q.Seq.d/                       1483   0.0
CHL132 (CHL132Q) /CSM/CH/CHL1-B/CHL132Q.Seq.d/                       1477   0.0
SHA612 (SHA612Q) /CSM/SH/SHA6-A/SHA612Q.Seq.d/                       1475   0.0
SHK714 (SHK714Q) /CSM/SH/SHK7-A/SHK714Q.Seq.d/                       1469   0.0
AHN166 (AHN166Q) /CSM/AH/AHN1-C/AHN166Q.Seq.d/                       1467   0.0
AHK681 (AHK681Q) /CSM/AH/AHK6-D/AHK681Q.Seq.d/                       1467   0.0
AHE434 (AHE434Q) /CSM/AH/AHE4-B/AHE434Q.Seq.d/                       1463   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.  1384   0.0    3
BI888273|BI888273.1 ZF637-2-000028 Zebrafish shield stage whole embryo cDNA library MPMGp637 Danio rerio cDNA clone MPMGp637_18D5;MPMGp637D0518 5', mRNA sequence.    52   0.043  1
CF446836|CF446836.1 EST683181 normalized cDNA library of onion Allium cepa cDNA clone ACAAS70, mRNA sequence.    50   0.17   1
CC571843|CC571843.1 CH240_447H2.TARBAC13P2 CHORI-240 Bos taurus genomic clone CH240_447H2, genomic survey sequence.    38   0.17   2
DU016203|DU016203.1 238566 Tomato MboI BAC Library Solanum lycopersicum genomic clone SL_MboI0039H16 5, genomic survey sequence.    36   0.21   3
AE014832|AE014832.1 Plasmodium falciparum 3D7 chromosome 10 section 4 of 7 of the complete sequence.    36   0.36   2
DC209380|DC209380.1 Plasmodium berghei strain ANKA cDNA clone:MG009860, midgut sporozoite 5'-EST.    46   0.43   2
BB976652|BB976652.1 Plasmodium berghei strain ANKA cDNA clone:OK006559, ookinete 5' EST.    46   0.57   2
BE555247|BE555247.1 sp87b08.y1 Gm-c1045 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1045-592 5' similar to TR:O64720 O64720 PUTATIVE TBP-BINDING PROTEIN. ;, mRNA sequence.    38   0.66   2
AM180355|AM180355.1 Clostridium difficile 630 complete genome.     48   0.66   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86KD1) RecName: Full=Cullin-associated NEDD8-dissociated prote...   426   e-119
BC004232_1(BC004232|pid:none) Homo sapiens cullin-associated and...   197   6e-49
AK001791_1(AK001791|pid:none) Homo sapiens cDNA FLJ10929 fis, cl...   197   6e-49
AB179357_1(AB179357|pid:none) Macaca fascicularis testis cDNA cl...   197   6e-49
BC026220_1(BC026220|pid:none) Homo sapiens cullin-associated and...   197   6e-49
AB020636_1(AB020636|pid:none) Homo sapiens mRNA for KIAA0829 pro...   197   6e-49
AK292456_1(AK292456|pid:none) Homo sapiens cDNA FLJ77762 complet...   197   6e-49
AL833880_1(AL833880|pid:none) Homo sapiens mRNA; cDNA DKFZp434P1...   197   6e-49
(A7MBJ5) RecName: Full=Cullin-associated NEDD8-dissociated prote...   197   6e-49
(Q6ZQ38) RecName: Full=Cullin-associated NEDD8-dissociated prote...   197   1e-48

 psg: 0.90  gvh: 0.44  alm: 0.36  top: 0.40  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for VHB862 is cyt

>VHB862F.Seq
ATTCAATTGTGTGATTGTGTTGTGTGTGTATTTTGGTTGATACACATTATATATATATAT
AATACAATAAAATGTCATTTTTTTTAGGACAGATTTTAGAAAAGATGGGTTCCATCGATA
AAGATATTAGATTCATGGCAACTCATGATCTTGCAAATGAATTAGAAAAAGATACTTTTA
AAATGGATCCAACTTATGAAAATAAAATTGTTACAAAATTATTAGCATTAACAGCTGATT
CAGCAAATAATGTTCAAGAAAATGTTGTTAAATGTTTAGGTTTATTAATTAAAAGAGTTA
AAGATTCACAAGCAACAGAAATTATTGATACATTATCAAAAAATATTTTGGAAGAATCAA
ATAAAGAAGAATTAGTTGAAATTTCAGGTATTGGTTTAAAAACAATCATTACAAATTTAC
CAAGTGAAGGTAGTTCAATTTCAACTTTAGTAATTAAAAATTTAGTACCAAAATTATTAA
TTGGTATTGATAGTGAAAAATTAAAAGATAAAAATGAAATTAAAATGTCATGTTTAGATA
TTTTAAATGATTTATTACAAAAGTATGGATCATTTATGATTGGAGATTTAGAGAATANNN
NNNNNNN

>VHB862Z.Seq
NNNNNNNNNNCAAATCACCATCACCATTGGAACGTTCAACCATTGGTAACCTCAATTAAA
TTCTCAATCATGGAGAATAAAGAAGTTGTCGATCAATATTTAGCTCCAAACATCTCTCAA
TTCCTTTCACTCTTACATGATGGTGATTTAATTGTTAGACGTAGTGCCCTCCTTTCATTG
AATTACATTGCTCACAATAAACCAAATCTCATTCGTAATGATCTCTCTGTCTATTTACCA
ATCTTGTACAACAATGCTAAAATCAAACCAGAACTCATTCGTGAAGTTGATCTTGGTCCA
TTCAAACATAAGGTCGATGATGGTATTGAAATCAGAAAAACAGCTTTCGAATGTATGTAC
ACTCTTTTGGATACCTCTATCGATAAGATCGATGTAGCTCCATTCATCGTTTCATTATGT
GATGGTCTCAAAGATACCCAATACGATATCAAATTGTTATGTCATTTAATGATCATTCGT
TTAGCAAACTCAAATGGTGCTGCTCTCCTCGAAAATATTACTCTCCTCCTTGAACCACTT
CGTGTTATTCTCATGACTAAAGTAAATGAAACCGCTGTTAAACAACAAATCGAACGTAAT
GAAGAATGTATTCGTTCAGCTTTACGTGCTGTCGCTTCCATCTCTAGAATTCCAAATAGT
GATAGTATCGTTAAATTTGAAGAATTTGTTAAAAATACAATTCGTACAACTCCATTAGCT
GCTCAATTTAATTCAATTTTATCGAAGATACAATGTCAAATCAAGATTCA

>VHB862P.Seq
ATTCAATTGTGTGATTGTGTTGTGTGTGTATTTTGGTTGATACACATTATATATATATAT
AATACAATAAAATGTCATTTTTTTTAGGACAGATTTTAGAAAAGATGGGTTCCATCGATA
AAGATATTAGATTCATGGCAACTCATGATCTTGCAAATGAATTAGAAAAAGATACTTTTA
AAATGGATCCAACTTATGAAAATAAAATTGTTACAAAATTATTAGCATTAACAGCTGATT
CAGCAAATAATGTTCAAGAAAATGTTGTTAAATGTTTAGGTTTATTAATTAAAAGAGTTA
AAGATTCACAAGCAACAGAAATTATTGATACATTATCAAAAAATATTTTGGAAGAATCAA
ATAAAGAAGAATTAGTTGAAATTTCAGGTATTGGTTTAAAAACAATCATTACAAATTTAC
CAAGTGAAGGTAGTTCAATTTCAACTTTAGTAATTAAAAATTTAGTACCAAAATTATTAA
TTGGTATTGATAGTGAAAAATTAAAAGATAAAAATGAAATTAAAATGTCATGTTTAGATA
TTTTAAATGATTTATTACAAAAGTATGGATCATTTATGATTGGAGATTTAGAGAATA---
-------CAAATCACCATCACCATTGGAACGTTCAACCATTGGTAACCTCAATTAAATTC
TCAATCATGGAGAATAAAGAAGTTGTCGATCAATATTTAGCTCCAAACATCTCTCAATTC
CTTTCACTCTTACATGATGGTGATTTAATTGTTAGACGTAGTGCCCTCCTTTCATTGAAT
TACATTGCTCACAATAAACCAAATCTCATTCGTAATGATCTCTCTGTCTATTTACCAATC
TTGTACAACAATGCTAAAATCAAACCAGAACTCATTCGTGAAGTTGATCTTGGTCCATTC
AAACATAAGGTCGATGATGGTATTGAAATCAGAAAAACAGCTTTCGAATGTATGTACACT
CTTTTGGATACCTCTATCGATAAGATCGATGTAGCTCCATTCATCGTTTCATTATGTGAT
GGTCTCAAAGATACCCAATACGATATCAAATTGTTATGTCATTTAATGATCATTCGTTTA
GCAAACTCAAATGGTGCTGCTCTCCTCGAAAATATTACTCTCCTCCTTGAACCACTTCGT
GTTATTCTCATGACTAAAGTAAATGAAACCGCTGTTAAACAACAAATCGAACGTAATGAA
GAATGTATTCGTTCAGCTTTACGTGCTGTCGCTTCCATCTCTAGAATTCCAAATAGTGAT
AGTATCGTTAAATTTGAAGAATTTGTTAAAAATACAATTCGTACAACTCCATTAGCTGCT
CAATTTAATTCAATTTTATCGAAGATACAATGTCAAATCAAGATTCA