>VHB852 (VHB852Q) /CSM/VH/VHB8-C/VHB852Q.Seq.d/
GAATNAACTCTTGTTGTTTGTTTGAAAATCAAATCAATTTCCTTTAAAGATAGTTTTTTA
AAGAAAAATGGCACCACCAACAAAAGAATTAACAAATAAAGAGATTACAGCAATCAAGGG
TATTTCAAAATTATTTGATGATAAGAAATACAGTAAAGGTTTAAAAGAGTGTAATGCAAT
TTTAAAGAATCATCCAGAAAATCCAGATGCATCATGTTTTAAGAGTTTGATTTTATACAA
TTTAGAACAACAATATGAAGCACGTGAAAATGCAATCAAAACCACCAAAACCTTACCAAC
CAACTCAATGGCATGGCATACATTAGGTTACATTTATAAATTGGATAAGAACTATCTCGA
TGCAACTACAGCCTTTAAAAATGCTTCAAAATTCAATAAAGAAAGTTCACAAATCTTAAA
AGAATTAGCACCACTTCAATTATATTCAAGAGATTTAGAAGGTTTAAAGGAAACGTATGC
AACATTATTAAAATTACAACCAACTCATAAACCACATTGGACAGGATTAGTGACTACATA
TCAATTAATGGGACAAACTAAATCAGCATTATCAGTATTTGAAAGATATTTAGAXXXXXX
XXXXCATTCCAGAGGATGACGATTTGAATGGTAAAAAGTTCTTGGTTAAACAAGTTAATC
TCATGGATTCAGCTCAACTCATTTTAGATAATCTCTTGAAAAATACAAATGATATTGAAA
TTTATTCATTAGCTTGCCAAGTTAATACACATCAAAAGAAATATTTATTAGTATTAAAAT
CATTGGTTAAAATTAAATCAATTAATGAAAATAGTCCATGTTATTATGAAAATTTAGTTG
ATTTAATGATTAAAGTATCAGAAGATAAAGAGGTATCAGCAATTGTTAAAGCAGCAGTTG
AAAAACAAGCAGGTTTTTCATCAGATGTTGAATCAATTTTAGCAAACAACCAAACTTATC
TCGAAAAACTTGGTGACAATGTTACCGCTCAACATCGTTTCGTTGTTGGTAAAATCATTG
CCAAACTTTCACCATCATCACTTGATAAAGCATCAGAATTGATTTTGAATGTTCAAGGTG
AAACAAGTTGGAAACAATGCGCTGACAACCTTCAATCTCTTTCTCTTGTATCTTCCGAAC
TCACTTCTAAATACAGAGAAAAATTACACGAACATTTCCCAATTGCTAACTATTTCCAAC
CAAAAGTTGAAAATTAATTCAAAATAAAAAAAATAATAATAAGAATAATAAATAATAATC
AATTTAAAAATAAACTAATCAATAAAT

nxlllfv*ksnqfplkivf*RKMAPPTKELTNKEITAIKGISKLFDDKKYSKGLKECNAI
LKNHPENPDASCFKSLILYNLEQQYEARENAIKTTKTLPTNSMAWHTLGYIYKLDKNYLD
ATTAFKNASKFNKESSQILKELAPLQLYSRDLEGLKETYATLLKLQPTHKPHWTGLVTTY
QLMGQTKSALSVFERYL---

---IPEDDDLNGKKFLVKQVNLMDSAQLILDNLLKNTNDIEIYSLACQVNTHQKKYLLVL
KSLVKIKSINENSPCYYENLVDLMIKVSEDKEVSAIVKAAVEKQAGFSSDVESILANNQT
YLEKLGDNVTAQHRFVVGKIIAKLSPSSLDKASELILNVQGETSWKQCADNLQSLSLVSS
ELTSKYREKLHEHFPIANYFQPKVEN*fkikkiiiriinnnqfknklink

Frame A:
extlvvclkiksisfkdsflkkngttnkrink*rdysnqgyfkii***eiq*rfkrv*cn
fkessrksrcimf*efdfiqfrtti*st*kcnqnhqnltnqlngmayirlhl*ig*elsr
cnysl*kcfkiq*rkftnlkristtsiifkrfrrfkgnvcniikittns*ttldrisdyi
singtn*isiisi*kifr---

---hsrg*rfew*kvlg*ts*shgfssthfr*slekyk*y*nlfislps*ytskeifisi
kiig*n*in**k*smll*kfs*fnd*sirr*rgisnc*sss*ktsrffirc*infskqpn
lsrktw*qcyrstsfrcw*nhcqtftiit**siridfecsr*nkletmr*qpsisfscif
rthf*iqrkitrtfpnc*lfptks*kliqnkknnnknnk**si*k*tnq*

Frame B:
nxlllfv*ksnqfplkivf*RKMAPPTKELTNKEITAIKGISKLFDDKKYSKGLKECNAI
LKNHPENPDASCFKSLILYNLEQQYEARENAIKTTKTLPTNSMAWHTLGYIYKLDKNYLD
ATTAFKNASKFNKESSQILKELAPLQLYSRDLEGLKETYATLLKLQPTHKPHWTGLVTTY
QLMGQTKSALSVFERYL---

---IPEDDDLNGKKFLVKQVNLMDSAQLILDNLLKNTNDIEIYSLACQVNTHQKKYLLVL
KSLVKIKSINENSPCYYENLVDLMIKVSEDKEVSAIVKAAVEKQAGFSSDVESILANNQT
YLEKLGDNVTAQHRFVVGKIIAKLSPSSLDKASELILNVQGETSWKQCADNLQSLSLVSS
ELTSKYREKLHEHFPIANYFQPKVEN*fkikkiiiriinnnqfknklink

Frame C:
xnscclfenqinfl*r*ffkekwhhqqkn*qikrlqqsrvfqnylmirntvkv*ksvmqf
*riiqkiqmhhvlrv*fyti*nnnmkhvkmqskppkpyqptqwhgih*vtfinwirtism
qlqplkmlqnsikkvhks*kn*hhfnyiqei*kv*rkrmqhy*nynqlinhigqd**lhi
n*wdklnqhyqylkdi*---

---fqrmti*mvksswlnkliswiqlnsf*iis*kiqmilkfih*laklihikrniy*y*
nhwlklnqlmkivhvimki*li**lkyqkikryqqllkqqlknkqvfhqmlnqf*qttkl
isknlvtmlplnivsllvkslpnfhhhhlikhqn*f*mfkvkqvgnnalttfnlfllylp
nsllnteknytnisqlltisnqklkinsk*kk***e**iiinlkin*sin

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB852 (VHB852Q) /CSM/VH/VHB8-C/VHB852Q.Seq.d/                       2351   0.0
VHQ454 (VHQ454Q) /CSM/VH/VHQ4-C/VHQ454Q.Seq.d/                       1219   0.0
VHO636 (VHO636Q) /CSM/VH/VHO6-B/VHO636Q.Seq.d/                       1219   0.0
VHO243 (VHO243Q) /CSM/VH/VHO2-B/VHO243Q.Seq.d/                       1219   0.0
VHJ545 (VHJ545Q) /CSM/VH/VHJ5-B/VHJ545Q.Seq.d/                       1219   0.0
VHA756 (VHA756Q) /CSM/VH/VHA7-C/VHA756Q.Seq.d/                       1219   0.0
VHA488 (VHA488Q) /CSM/VH/VHA4-D/VHA488Q.Seq.d/                       1219   0.0
VHA402 (VHA402Q) /CSM/VH/VHA4-A/VHA402Q.Seq.d/                       1219   0.0
VHD223 (VHD223Q) /CSM/VH/VHD2-A/VHD223Q.Seq.d/                       1213   0.0
VHB251 (VHB251Q) /CSM/VH/VHB2-C/VHB251Q.Seq.d/                       1213   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   882   0.0    5
AC161408|AC161408.4 Mus musculus chromosome 1, clone RP24-329G10, complete sequence.    44   0.005  5
AC120400|AC120400.11 Mus musculus chromosome 1, clone RP24-351P4, complete sequence.    44   0.024  7
CR457451|CR457451.2 Zebrafish DNA sequence *** SEQUENCING CANCELLED *** from clone  CH211-197B3.    52   0.040  1
BX511270|BX511270.10 Zebrafish DNA sequence from clone DKEY-35N12 in linkage group 14.    52   0.040  1
AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    32   0.069  14
AE017143|AE017143.1 Haemophilus ducreyi strain 35000HP complete genome.    36   0.083  2
AM285304|AM285304.1 Spiroplasma citri GII3-3X chromosome, contig Contig03.    34   0.099  13
AE017332|AE017332.1 Mycoplasma hyopneumoniae 232, complete genome.    34   0.12   22
AZ539456|AZ539456.1 ENTDG45TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    38   0.12   2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116305_76(AC116305|pid:none) Dictyostelium discoideum chromoso...   360   5e-98
EU952143_1(EU952143|pid:none) Zea mays clone 1211771 hypothetica...   109   2e-22
AK118131_1(AK118131|pid:none) Arabidopsis thaliana At1g80410 mRN...   107   7e-22
BC142456_1(BC142456|pid:none) Bos taurus NMDA receptor regulated...   107   9e-22
BX640665_1(BX640665|pid:none) Homo sapiens mRNA; cDNA DKFZp686O0...   106   2e-21
(Q6N069) RecName: Full=NMDA receptor-regulated 1-like protein;  ...   106   2e-21
AP007155_890(AP007155|pid:none) Aspergillus oryzae RIB40 genomic...   104   8e-21
AC018849_25(AC018849|pid:none) Arabidopsis thaliana chromosome 1...   103   1e-20
AY391464_1(AY391464|pid:none) Danio rerio transcriptional coacti...   103   2e-20
BC044392_1(BC044392|pid:none) Danio rerio NMDA receptor-regulate...   103   2e-20

 psg: 0.63  gvh: 0.23  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall

>> prediction for VHB852 is nuc

>VHB852F.Seq
GAATNAACTCTTGTTGTTTGTTTGAAAATCAAATCAATTTCCTTTAAAGATAGTTTTTTA
AAGAAAAATGGCACCACCAACAAAAGAATTAACAAATAAAGAGATTACAGCAATCAAGGG
TATTTCAAAATTATTTGATGATAAGAAATACAGTAAAGGTTTAAAAGAGTGTAATGCAAT
TTTAAAGAATCATCCAGAAAATCCAGATGCATCATGTTTTAAGAGTTTGATTTTATACAA
TTTAGAACAACAATATGAAGCACGTGAAAATGCAATCAAAACCACCAAAACCTTACCAAC
CAACTCAATGGCATGGCATACATTAGGTTACATTTATAAATTGGATAAGAACTATCTCGA
TGCAACTACAGCCTTTAAAAATGCTTCAAAATTCAATAAAGAAAGTTCACAAATCTTAAA
AGAATTAGCACCACTTCAATTATATTCAAGAGATTTAGAAGGTTTAAAGGAAACGTATGC
AACATTATTAAAATTACAACCAACTCATAAACCACATTGGACAGGATTAGTGACTACATA
TCAATTAATGGGACAAACTAAATCAGCATTATCAGTATTTGAAAGATATTTAGANNNNNN
NNNN

>VHB852Z.Seq
NNNNNNNNNNCATTCCAGAGGATGACGATTTGAATGGTAAAAAGTTCTTGGTTAAACAAG
TTAATCTCATGGATTCAGCTCAACTCATTTTAGATAATCTCTTGAAAAATACAAATGATA
TTGAAATTTATTCATTAGCTTGCCAAGTTAATACACATCAAAAGAAATATTTATTAGTAT
TAAAATCATTGGTTAAAATTAAATCAATTAATGAAAATAGTCCATGTTATTATGAAAATT
TAGTTGATTTAATGATTAAAGTATCAGAAGATAAAGAGGTATCAGCAATTGTTAAAGCAG
CAGTTGAAAAACAAGCAGGTTTTTCATCAGATGTTGAATCAATTTTAGCAAACAACCAAA
CTTATCTCGAAAAACTTGGTGACAATGTTACCGCTCAACATCGTTTCGTTGTTGGTAAAA
TCATTGCCAAACTTTCACCATCATCACTTGATAAAGCATCAGAATTGATTTTGAATGTTC
AAGGTGAAACAAGTTGGAAACAATGCGCTGACAACCTTCAATCTCTTTCTCTTGTATCTT
CCGAACTCACTTCTAAATACAGAGAAAAATTACACGAACATTTCCCAATTGCTAACTATT
TCCAACCAAAAGTTGAAAATTAATTCAAAATAAAAAAAATAATAATAAGAATAATAAATA
ATAATCAATTTAAAAATAAACTAATCAATAAAT

>VHB852P.Seq
GAATNAACTCTTGTTGTTTGTTTGAAAATCAAATCAATTTCCTTTAAAGATAGTTTTTTA
AAGAAAAATGGCACCACCAACAAAAGAATTAACAAATAAAGAGATTACAGCAATCAAGGG
TATTTCAAAATTATTTGATGATAAGAAATACAGTAAAGGTTTAAAAGAGTGTAATGCAAT
TTTAAAGAATCATCCAGAAAATCCAGATGCATCATGTTTTAAGAGTTTGATTTTATACAA
TTTAGAACAACAATATGAAGCACGTGAAAATGCAATCAAAACCACCAAAACCTTACCAAC
CAACTCAATGGCATGGCATACATTAGGTTACATTTATAAATTGGATAAGAACTATCTCGA
TGCAACTACAGCCTTTAAAAATGCTTCAAAATTCAATAAAGAAAGTTCACAAATCTTAAA
AGAATTAGCACCACTTCAATTATATTCAAGAGATTTAGAAGGTTTAAAGGAAACGTATGC
AACATTATTAAAATTACAACCAACTCATAAACCACATTGGACAGGATTAGTGACTACATA
TCAATTAATGGGACAAACTAAATCAGCATTATCAGTATTTGAAAGATATTTAGA------
----CATTCCAGAGGATGACGATTTGAATGGTAAAAAGTTCTTGGTTAAACAAGTTAATC
TCATGGATTCAGCTCAACTCATTTTAGATAATCTCTTGAAAAATACAAATGATATTGAAA
TTTATTCATTAGCTTGCCAAGTTAATACACATCAAAAGAAATATTTATTAGTATTAAAAT
CATTGGTTAAAATTAAATCAATTAATGAAAATAGTCCATGTTATTATGAAAATTTAGTTG
ATTTAATGATTAAAGTATCAGAAGATAAAGAGGTATCAGCAATTGTTAAAGCAGCAGTTG
AAAAACAAGCAGGTTTTTCATCAGATGTTGAATCAATTTTAGCAAACAACCAAACTTATC
TCGAAAAACTTGGTGACAATGTTACCGCTCAACATCGTTTCGTTGTTGGTAAAATCATTG
CCAAACTTTCACCATCATCACTTGATAAAGCATCAGAATTGATTTTGAATGTTCAAGGTG
AAACAAGTTGGAAACAATGCGCTGACAACCTTCAATCTCTTTCTCTTGTATCTTCCGAAC
TCACTTCTAAATACAGAGAAAAATTACACGAACATTTCCCAATTGCTAACTATTTCCAAC
CAAAAGTTGAAAATTAATTCAAAATAAAAAAAATAATAATAAGAATAATAAATAATAATC
AATTTAAAAATAAACTAATCAATAAAT