>VHB846 (VHB846Q) /CSM/VH/VHB8-B/VHB846Q.Seq.d/
ATTATTGTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTG
GTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAA
TTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATACAG
AACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTAT
TTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATATGA
AACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGAAT
TCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAAAAA
TGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGTTC
CAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAAAAT
TAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTTAXXXX
XXXXXXGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACC
AAAGATAATAATTTATTGGGTAAATTTGNACTTTCTGGTATTCCACCAGCACCAAGAGGT
GTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATNTTAAATGTATCAGCT
GAAGATAAATCAACTGGTAACNAACAAAAGATTACNATTACCAATGATAAAGGTCGTNTT
TCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCCAACAACAAGATGAN
CANCAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAA
AATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATT
GAATNTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGAT
GAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTC
TATCAAGAAGGAGGTATGCCNCAAGGTGGTGGTANGCCAGGTGGTATGTCAAATGATTCN
CCAAAGAGTTCNAACAACAAAGTAGCCGAATTAGATTGACGGAATTTTTCAGAGGAAAAC
ATCTCTATCAAATAGATTTCTTAAAAAATAATNAGTTAGTAAAATCAATAAAGCTTCTCA

yc*nksky*iiky*KMSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDTE
RLIGDAAKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEF
KGETKVFSPEEISSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKL
NVQRIINEPTAAAIAYGL---

---DNQPGVLIQVYEGERAMTKDNNLLGKFXLSGIPPAPRGVPQVEVTFDVDANGXLNVS
AEDKSTGNXQKITITNDKGRXSKEEIEKMVADAEKFQQQDXXQKDRVESKNKLENYAFTV
KNSIKDEKVAAKISDSDKSTIEXETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMSK
LYQEGGMPQGGGXPGGMSNDSPKSSNNKVAELD*rnfseenisik*is*kixs**nq*sf
s

Frame A:
iivkirvnik*lnikkchqlvli*vqhihvlvfgkmivlkllqmikvieplhlmlhlliq
nv*lvmlqriklq*iqpiqylmlnv*lvvnlvtkkfkvi*nighsklfqrmvishifkln
skvklkfshqkkslqwys*k*kkplklilvkpliml*lpfqlismivnvkqpkmlvqfqn
*mfnvllmnqllllllmv---

---DNQPGVLIQVYEGERAMTKDNNLLGKFXLSGIPPAPRGVPQVEVTFDVDANGXLNVS
AEDKSTGNXQKITITNDKGRXSKEEIEKMVADAEKFQQQDXXQKDRVESKNKLENYAFTV
KNSIKDEKVAAKISDSDKSTIEXETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMSK
LYQEGGMPQGGGXPGGMSNDSPKSSNNKVAELD*rnfseenisik*is*kixs**nq*sf
s

Frame B:
lllk*e*ilnn*ilknvinwy*frynifmcwclak*sc*nyck*sr**nhsilcciy*yr
tfnw*cckesscnesnqysi*c*tfnws*i**qrssk*yetlaiqsyskgw**atyss*i
qr*n*sfltrrnlfngtlknernr*slsw*nh**ccnyrsslfq**stssnqrcwynfki
kcstyy**tncccyclwf---

---iinqvy*fkfmkvnvl*pkiiiywvnlxflvfhqhqevyhklklhlmlmqmvx*myq
lkinqlvtnkrlxlpmikvvfqkkklkkwllmqrnsnnkmxxkkivlnqrinwkimhsll
kiqlkmkklqpkfqiqinqllnxklkvysng*nqikpqkrmnmkir*kp*kqlsiqs*vn
sikkevcxkvvvxqvvcqmixqrvxttk*pn*idgifqrktslsnrflkk*xvskinkas

Frame C:
yc*nksky*iiky*KMSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDTE
RLIGDAAKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEF
KGETKVFSPEEISSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKL
NVQRIINEPTAAAIAYGL---

---*strcinssl*r*tcydqr**fig*ixtfwyststkrctts*syi*c*ckwyxkcis
*r*inw*xtkdyxyq**rsxfkrrn*kngc*creipttr*xxkrsc*ike*igklcihc*
kfn*r*kscsqnfrfr*iny*x*n*kctqmvriksnrrkg*i*r*deslrsscqsnhe*t
lsrrryaxrwwxarwyvk*fxkefxqqssrirlteffrgkhlyqidflknnxlvksikll

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB846 (VHB846Q) /CSM/VH/VHB8-B/VHB846Q.Seq.d/                       2383   0.0
VHN672 (VHN672Q) /CSM/VH/VHN6-C/VHN672Q.Seq.d/                       2361   0.0
VHQ260 (VHQ260Q) /CSM/VH/VHQ2-C/VHQ260Q.Seq.d/                       1322   0.0
VHP719 (VHP719Q) /CSM/VH/VHP7-A/VHP719Q.Seq.d/                       1322   0.0
VHP716 (VHP716Q) /CSM/VH/VHP7-A/VHP716Q.Seq.d/                       1322   0.0
VHP437 (VHP437Q) /CSM/VH/VHP4-B/VHP437Q.Seq.d/                       1322   0.0
VHP420 (VHP420Q) /CSM/VH/VHP4-A/VHP420Q.Seq.d/                       1322   0.0
VHP236 (VHP236Q) /CSM/VH/VHP2-B/VHP236Q.Seq.d/                       1322   0.0
VHP232 (VHP232Q) /CSM/VH/VHP2-B/VHP232Q.Seq.d/                       1322   0.0
VHP224 (VHP224Q) /CSM/VH/VHP2-A/VHP224Q.Seq.d/                       1322   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).  1320   0.0    1
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.  1312   0.0    1
AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.   285   e-161  5
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   285   e-152  7
AF221541|AF221541.1 Cryptosporidium serpentis heat shock protein 70 (HSP70) gene, partial cds.   145   e-118  10
A58313|A58313.1 Sequence 1 from Patent WO9636707.                 123   e-118  9
Z30210|Z30210.1 C.albicans (ATCC 20955) cahsp70 mRNA for heat shock protein 70.   123   e-117  9
AX536934|AX536934.1 Sequence 535 from Patent WO02064766.          123   e-117  9
AR941672|AR941672.1 Sequence 535 from patent US 7101990.          123   e-117  9
AY954892|AY954892.1 Cryptosporidium andersoni strain HenanZhzhca heat shock protein 70 gene, partial cds.   123   e-112  10

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   360   8e-98
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   358   3e-97
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   338   3e-91
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   338   3e-91
(Q54BE0) RecName: Full=Heat shock cognate 70 kDa protein 3;           320   9e-86
U71151_1(U71151|pid:none) Pneumocystis carinii heat shock protei...   315   2e-84
AY495382_1(AY495382|pid:none) Trypanosoma rangeli isolate Tre he...   315   2e-84
U80967_1(U80967|pid:none) Pneumocystis carinii f. sp. carinii he...   315   2e-84
(P11145) RecName: Full=Heat shock 70 kDa protein 4; AltName: Ful...   313   7e-84
EF442767_1(EF442767|pid:none) Trypanosoma rangeli isolate H14 cl...   313   1e-83

 psg: 0.87  gvh: 0.35  alm: 0.46  top: 0.27  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHB846 is cyt

>VHB846F.Seq
ATTATTGTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTG
GTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAA
TTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATACAG
AACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTAT
TTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATATGA
AACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGAAT
TCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAAAAA
TGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGTTC
CAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAAAAT
TAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTTANNNN
NNNNNN

>VHB846Z.Seq
NNNNNNNNNNGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTAT
GACCAAAGATAATAATTTATTGGGTAAATTTGNACTTTCTGGTATTCCACCAGCACCAAG
AGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATNTTAAATGTATC
AGCTGAAGATAAATCAACTGGTAACNAACAAAAGATTACNATTACCAATGATAAAGGTCG
TNTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCCAACAACAAGA
TGANCANCAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGT
TAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAAC
TATTGAATNTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAA
GGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAA
ACTCTATCAAGAAGGAGGTATGCCNCAAGGTGGTGGTANGCCAGGTGGTATGTCAAATGA
TTCNCCAAAGAGTTCNAACAACAAAGTAGCCGAATTAGATTGACGGAATTTTTCAGAGGA
AAACATCTCTATCAAATAGATTTCTTAAAAAATAATNAGTTAGTAAAATCAATAAAGCTT
CTCA

>VHB846P.Seq
ATTATTGTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTG
GTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAA
TTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATACAG
AACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTAT
TTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATATGA
AACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGAAT
TCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAAAAA
TGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGTTC
CAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAAAAT
TAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTTA----
------GATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACC
AAAGATAATAATTTATTGGGTAAATTTGNACTTTCTGGTATTCCACCAGCACCAAGAGGT
GTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATNTTAAATGTATCAGCT
GAAGATAAATCAACTGGTAACNAACAAAAGATTACNATTACCAATGATAAAGGTCGTNTT
TCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCCAACAACAAGATGAN
CANCAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAA
AATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATT
GAATNTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGAT
GAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTC
TATCAAGAAGGAGGTATGCCNCAAGGTGGTGGTANGCCAGGTGGTATGTCAAATGATTCN
CCAAAGAGTTCNAACAACAAAGTAGCCGAATTAGATTGACGGAATTTTTCAGAGGAAAAC
ATCTCTATCAAATAGATTTCTTAAAAAATAATNAGTTAGTAAAATCAATAAAGCTTCTCA