>VHB845 (VHB845Q) /CSM/VH/VHB8-B/VHB845Q.Seq.d/
XXXXXXXXXXTATGTCAAGAAAATATAATGAAGCCACTAAAGCTTTAAATACAATTTTAG
TTTCTGTTGCTTCAAAATCAAAATNTCAAAATTTCCAATNTGATCATAATGAGAAAAAAA
CAGATAAAATGTATGCATTATTAACAATTTGTCAAGCACTTTATCCAAATAAGATTGATG
AAAATGTTAGTAATAATTTGAAAGAGAAATTTGGTGATAAATTATTAAGATTACAAAAGG
GTGATCTTTCAGTTTATGAGGAATTGTATACATTTGCTGCCCCACGTTGTATCGGTCCAG
TACCACCATACATTTCAGGTTTCAATGTAACCGCTTTGGATCCACATCGTCTTCAATTAT
CATTGTTTTTAGAGGAGGTCAAACAACAATCCCTCTTGACAACCATTNGTAGTTATATCA
CTTTGTATTCAACTATCTCCATTCAAAAGTTGGCTGGTTTACTTCAAATCGATCCAGAGG
ATTTACGTGTTAAACTCACTTGTTACAAACATAAATTATTCAACGTTATAGGTAAAGGTG
AAACTAAATGGAATAATCAATTGGATATCGATTTNTACATTGATCAAGATATGATCCATA
TCGATAATCGTCTTTATGCCNAAGAAACTGATTCCTTCATTAATTCAATTCTTAAATTCG
AAGGTTCAT

---MSRKYNEATKALNTILVSVASKSKXQNFQXDHNEKKTDKMYALLTICQALYPNKIDE
NVSNNLKEKFGDKLLRLQKGDLSVYEELYTFAAPRCIGPVPPYISGFNVTALDPHRLQLS
LFLEEVKQQSLLTTIXSYITLYSTISIQKLAGLLQIDPEDLRVKLTCYKHKLFNVIGKGE
TKWNNQLDIDXYIDQDMIHIDNRLYAXETDSFINSILKFEGS

Frame A:
---MSRKYNEATKALNTILVSVASKSKXQNFQXDHNEKKTDKMYALLTICQALYPNKIDE
NVSNNLKEKFGDKLLRLQKGDLSVYEELYTFAAPRCIGPVPPYISGFNVTALDPHRLQLS
LFLEEVKQQSLLTTIXSYITLYSTISIQKLAGLLQIDPEDLRVKLTCYKHKLFNVIGKGE
TKWNNQLDIDXYIDQDMIHIDNRLYAXETDSFINSILKFEGS


Frame B:
---cqenimkplkl*iqf*flllqnqnxkisnxiimrkkqikcmhy*qfvkhfiqirlmk
mlvii*krnlviny*dykrvifqfmrncihllphvvsvqyhhtfqvsm*plwihivfnyh
cf*rrsnnnps*qpxvvislciqlspfkswlvyfksiqriyvlnslvtninystl*vkvk
lngiinwisixtliki*sisiivfmpkklipsliqflnskvh


Frame C:
---vkki**sh*sfkynfsfccfkikxskfpx*s**eknr*nvciinnlsstlsk*d**k
c***fereiw**iikitkg*sfsl*givyiccptlyrssttihfrfqcnrfgstsssiii
vfrggqttipldnhx*lyhfvfnylhskvgwftsnrsrgftc*thllqt*iiqryr*r*n
*me*sigyrfxh*srydpyr*sslcxrn*flh*fns*irrf

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB845 (VHB845Q) /CSM/VH/VHB8-B/VHB845Q.Seq.d/                       1235   0.0
VHB839 (VHB839Q) /CSM/VH/VHB8-B/VHB839Q.Seq.d/                       1219   0.0
AFN332 (AFN332Q) /CSM/AF/AFN3-B/AFN332Q.Seq.d/                       1219   0.0
VFI648 (VFI648Q) /CSM/VF/VFI6-B/VFI648Q.Seq.d/                       1197   0.0
VHA720 (VHA720Q) /CSM/VH/VHA7-A/VHA720Q.Seq.d/                       1195   0.0
AFB344 (AFB344Q) /CSM/AF/AFB3-B/AFB344Q.Seq.d/                       1189   0.0
SFA330 (SFA330Q) /CSM/SF/SFA3-B/SFA330Q.Seq.d/                       1144   0.0
AFB869 (AFB869Q) /CSM/AF/AFB8-C/AFB869Q.Seq.d/                       1124   0.0
VSB836 (VSB836Q) /CSM/VS/VSB8-B/VSB836Q.Seq.d/                       1055   0.0
CFA631 (CFA631Q) /CSM/CF/CFA6-B/CFA631Q.Seq.d/                        999   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115604|AC115604.2 Dictyostelium discoideum chromosome 2 map 4354771-4414991 strain AX4, complete sequence.  1055   0.0    2
EC760482|EC760482.1 PSE00000748 rw_mgpallid Polysphondylium pallidum cDNA, mRNA sequence.    56   0.001  1
AJ236306|AJ236306.1 Pseudomussaenda flava chloroplast ndhF gene.    44   0.50   2
AC174086|AC174086.2 Strongylocentrotus purpuratus clone R3-56D18, WORKING DRAFT SEQUENCE, 7 unordered pieces.    40   0.77   4
DY605186|DY605186.1 IMMUNEF045816M22 POSSUM_01-C-POSSUM-IMMUNE-2KB Trichosurus vulpecula cDNA clone 1061021065604, mRNA sequence.    46   1.3    1
EC307169|EC307169.1 OVARYF089559N15 POSSUM_01-POSSUM-C-OVARY-2KB Trichosurus vulpecula cDNA clone 1061024729366, mRNA sequence.    46   1.3    1
AC163582|AC163582.2 Bos taurus clone CH240-132A20, *** SEQUENCING IN PROGRESS ***, 24 unordered pieces.    46   1.3    1
EJ910321|EJ910321.1 1093018534780 Global-Ocean-Sampling_GS-30-02-01-1P0-1P3KB marine metagenome genomic clone 1061002822915 3', genomic survey sequence.    46   1.3    1
EJ818958|EJ818958.1 1093017528800 Global-Ocean-Sampling_GS-30-02-01-1P0-1P3KB marine metagenome genomic clone 1061002822915 5', genomic survey sequence.    46   1.3    1
DU044852|DU044852.1 152508 Tomato HindIII BAC Library Solanum lycopersicum genomic clone LE_HBa0025C21 3, genomic survey sequence.    46   1.3    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86LA2) RecName: Full=Eukaryotic translation initiation factor ...   389   e-107
AY836660_1(AY836660|pid:none) Aurelia aurita clone 125 putative ...   140   4e-32
(Q6P878) RecName: Full=Eukaryotic translation initiation factor ...   137   3e-31
(Q7T2A5) RecName: Full=Eukaryotic translation initiation factor ...   135   8e-31
(Q5F428) RecName: Full=Eukaryotic translation initiation factor ...   134   2e-30
BC100264_1(BC100264|pid:none) Rattus norvegicus eukaryotic trans...   133   5e-30
AK167640_1(AK167640|pid:none) Mus musculus 14 days embryo liver ...   133   5e-30
(Q8QZY1) RecName: Full=Eukaryotic translation initiation factor ...   133   5e-30
AK027429_1(AK027429|pid:none) Homo sapiens cDNA FLJ14523 fis, cl...   132   1e-29
AK093540_1(AK093540|pid:none) Homo sapiens cDNA FLJ36221 fis, cl...   132   1e-29

 psg: 0.91  gvh: 0.45  alm: 0.41  top: 0.40  tms: 0.00  mit: 0.35  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 20.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for VHB845 is cyt

>VHB845Z.Seq
NNNNNNNNNNTATGTCAAGAAAATATAATGAAGCCACTAAAGCTTTAAATACAATTTTAG
TTTCTGTTGCTTCAAAATCAAAATNTCAAAATTTCCAATNTGATCATAATGAGAAAAAAA
CAGATAAAATGTATGCATTATTAACAATTTGTCAAGCACTTTATCCAAATAAGATTGATG
AAAATGTTAGTAATAATTTGAAAGAGAAATTTGGTGATAAATTATTAAGATTACAAAAGG
GTGATCTTTCAGTTTATGAGGAATTGTATACATTTGCTGCCCCACGTTGTATCGGTCCAG
TACCACCATACATTTCAGGTTTCAATGTAACCGCTTTGGATCCACATCGTCTTCAATTAT
CATTGTTTTTAGAGGAGGTCAAACAACAATCCCTCTTGACAACCATTNGTAGTTATATCA
CTTTGTATTCAACTATCTCCATTCAAAAGTTGGCTGGTTTACTTCAAATCGATCCAGAGG
ATTTACGTGTTAAACTCACTTGTTACAAACATAAATTATTCAACGTTATAGGTAAAGGTG
AAACTAAATGGAATAATCAATTGGATATCGATTTNTACATTGATCAAGATATGATCCATA
TCGATAATCGTCTTTATGCCNAAGAAACTGATTCCTTCATTAATTCAATTCTTAAATTCG
AAGGTTCAT