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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB821
Library VH
(Link to library)
Clone ID VHB821
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15687-1
Original site URL
Representative seq. ID VHB821P
(Link to Original site)
Representative DNA sequence 
>VHB821 (VHB821Q) /CSM/VH/VHB8-A/VHB821Q.Seq.d/
AATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTC
AAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGC
AACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAG
AAGAAGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAA
TTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAA
TTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAG
AAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAAT
CATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTAT
CAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGA
TCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTG
TCTATTATTCAATGATTGATATATTTTXXXXXXXXXXTAGAGAGAATAAAGAACAATTGA
ATAATATGTACCNTTCAGAGTTGGTGACAGAGTTGGAAAGAGACATTAGACAATTGGGTG
TCTCCGAGACTGGTTTAACCTTTTTAGATCATTTTAGACTTTTAATCACTCATATTGGTA
ATGCTATGGGTTATATTAGATTGGTCAGATCCGGTGGTTTACACTATTGTAGTAATGCTA
TCAAATTCGTACCAGACTTGAAAAAGATTCCAAAATTCCAAGATTTAACCTCAAAAGATG
CCCTCTCACCTGAAACAATTCAAGCTAGTACCAATTTGGATTCAATCATTCATAATCTTT
CAAATAATTTATCAGAGGGTACTGAATACTTTAAGATGTTGGTAAACGTTTTCGCTACAG
AGTTTCGAAATATCGCTAATCAACATTTAAAGAATTTCTATATCATCGTACCTGCCTTGG
CCTCAAATTACATCGATCATATGATCAATAGTAAAGATAAACTCTTTAAGAAATCAAAAG
CAATCGGTGCTGAAGCTTTATTATTCACTGATGATGGCTTTGCAATTGGTTTGGCTTATA
TCTTAAAATTATTAGATCAAAATAAAGATTTCGATTCTCTTCATTGGTTCGATCGTATTC
AATTGAAATGTGAAACTGATCAAAAGAGAATGTTGGCTGAAGCAAATGCAAAAGGTATCA
AAGAGGATCAAAATCATTTAGCAATTAAAAAGATTCAAAATTTCCAACAAGAATAGAATT
ATTTAAATATTCTTTCNCGGGTTCAAGA
sequence update 2002.10.25
Translated Amino Acid sequence 
*ktnnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIE
EALDETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILKE
TAESKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASI
YQSQKNIHTQFFKNVHLQAVYYSMIDIF---

---RENKEQLNNMYXSELVTELERDIRQLGVSETGLTFLDHFRLLITHIGNAMGYIRLVR
SGGLHYCSNAIKFVPDLKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNLSEGTEY
FKMLVNVFATEFRNIANQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGAEALLFT
DDGFAIGLAYILKLLDQNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKEDQNHLAIK
KIQNFQQE*nylnilsrvq
Translated Amino Acid sequence (All Frames) 
Frame A:
nkkrttqfrkwphpirlimvkmknihlmhlkmvhlnwlesnn*lnlinlqlimhnkfgr*
kkh*mkh*mkygi*i*iqfqfiinlmnn*i*lnwlkliikfsik*y*yslqfviklef*k
kqqkvnfihh*qylerlqvnhqkvmyklklvnyyhl*liyqhl*idvih*leillvnlhr
sinhkriyihnslrmfiyklsiiq*liyf---

---*re*rtie*yvpfrvgdrvgkrh*tigclrdwfnlfrsf*tfnhsyw*cygly*igq
irwftll**cyqirtrlekdskiprfnlkrcplt*nnss*yqfgfnhs*sfk*firgy*i
l*dvgkrfryrvskyr*stfkeflyhrtclglklhrsydq**r*tl*eiksnrc*sfiih
**wlcnwfglylkiirsk*rfrfsslvrsysiem*n*skenvg*skckryqrgsksfsn*
kdskfptrielfkysfxgsr

Frame B:
ikneqpnlenghilfg**wsk*riyi*ci*rwfi*igwratin*t*sics*lcttnledr
rsir*nik*smgykyrssfnl**ts*tirfn*ig*n*s*nfq*snisilfnl*sn*nfkr
nsrk*ilftinsiwrdyr*iirr*ctn*sw*iitiyd*fisick*mlfin*kyy*sicid
lsitkeytytil*ecsftscllfnd*yi---

---RENKEQLNNMYXSELVTELERDIRQLGVSETGLTFLDHFRLLITHIGNAMGYIRLVR
SGGLHYCSNAIKFVPDLKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNLSEGTEY
FKMLVNVFATEFRNIANQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGAEALLFT
DDGFAIGLAYILKLLDQNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKEDQNHLAIK
KIQNFQQE*nylnilsrvq

Frame C:
*ktnnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIE
EALDETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILKE
TAESKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASI
YQSQKNIHTQFFKNVHLQAVYYSMIDIF---

---eriknn*iictxqsw*qswketldnwvsprlv*pf*iildf*slilvmlwvildwsd
pvvytivvmlsnsyqt*krfqnski*pqkmpshlkqfklvpiwiqsfiifqiiyqrvlnt
lrcw*tfslqsfeislini*risissylpwpqitsii*sivkinslrnqkqsvlklyysl
mmalqlvwlis*ny*ikikisilfigsivfn*nvklikrecwlkqmqkvskrikii*qlk
rfkisnknrii*iffxgfk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB821 (VHB821Q) /CSM/VH/VHB8-A/VHB821Q.Seq.d/                       2720   0.0
AHN345 (AHN345Q) /CSM/AH/AHN3-B/AHN345Q.Seq.d/                       1516   0.0
SHD280 (SHD280Q) /CSM/SH/SHD2-D/SHD280Q.Seq.d/                       1509   0.0
VHB189 (VHB189Q) /CSM/VH/VHB1-D/VHB189Q.Seq.d/                       1503   0.0
VHB614 (VHB614Q) /CSM/VH/VHB6-A/VHB614Q.Seq.d/                       1501   0.0
VHA684 (VHA684Q) /CSM/VH/VHA6-D/VHA684Q.Seq.d/                       1501   0.0
CHG805 (CHG805Q) /CSM/CH/CHG8-A/CHG805Q.Seq.d/                       1501   0.0
VHQ112 (VHQ112Q) /CSM/VH/VHQ1-A/VHQ112Q.Seq.d/                       1495   0.0
VHJ241 (VHJ241Q) /CSM/VH/VHJ2-B/VHJ241Q.Seq.d/                       1495   0.0
VHI518 (VHI518Q) /CSM/VH/VHI5-A/VHI518Q.Seq.d/                       1495   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BU746157|BU746157.1 CH3#002_C07T7 Canine heart normalized cDNA Library in pBluescript Canis familiaris cDNA clone CH3#002_C07 5', mRNA sequence.    38   0.001  3
CP000263|CP000263.1 Buchnera aphidicola str. Cc (Cinara cedri), complete genome.    36   0.017  15
BY464171|BY464171.1 Mus musculus 15 days pregnant adult female amnion cDNA, RIKEN full-length enriched library, clone:K630150M24, 3' end partial sequence.    42   0.036  2
AK113047|AK113047.1 Ciona intestinalis cDNA, clone:ciad002a21, full insert sequence.    48   0.042  2
AC168241|AC168241.2 Bos taurus clone CH240-197K22, *** SEQUENCING IN PROGRESS ***, 28 unordered pieces.    52   0.044  1
BB822673|BB822673.1 Mus musculus mammary gland RCB-0526 Jyg-MC(A) cDNA, RIKEN full-length enriched library, clone:G830022O04, 3' end partial sequence.    32   0.15   4
DN287505|DN287505.1 1241755 MARC 7BOV Bos taurus cDNA 3', mRNA sequence.    34   0.17   3
CR103667|CR103667.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP142o16.    50   0.17   1
CR044130|CR044130.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP186g09.    50   0.17   1
CR034434|CR034434.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP47e19.    50   0.17   1
dna update 2007. 4.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54R74) RecName: Full=UPF0681 protein;                               491   e-137
BC040936_1(BC040936|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   241   6e-62
AB028956_1(AB028956|pid:none) Homo sapiens mRNA for KIAA1033 pro...   240   8e-62
BC143373_1(BC143373|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   240   8e-62
BC020314_1(BC020314|pid:none) Mus musculus RIKEN cDNA A230046K03...   238   3e-61
(Q3UMB9) RecName: Full=UPF0681 protein KIAA1033;                      238   3e-61
AK161247_1(AK161247|pid:none) Mus musculus adult male testis cDN...   238   3e-61
AK145000_1(AK145000|pid:none) Mus musculus mammary gland RCB-052...   238   3e-61
BC110850_1(BC110850|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   185   3e-45
AX882617_1(AX882617|pid:none) Sequence 17522 from Patent EP10746...   181   7e-44
protein update 2009. 2.24
PSORT 

 psg: 0.79  gvh: 0.25  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VHB821 is nuc
5' end seq. ID VHB821F
5' end seq. 
>VHB821F.Seq
AATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTC
AAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGC
AACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAG
AAGAAGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAA
TTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAA
TTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAG
AAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAAT
CATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTAT
CAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGA
TCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTG
TCTATTATTCAATGATTGATATATTTTNNNNNNNNNN
Length of 5' end seq. 637
3' end seq. ID VHB821Z
3' end seq. 
>VHB821Z.Seq
NNNNNNNNNNTAGAGAGAATAAAGAACAATTGAATAATATGTACCNTTCAGAGTTGGTGA
CAGAGTTGGAAAGAGACATTAGACAATTGGGTGTCTCCGAGACTGGTTTAACCTTTTTAG
ATCATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGGTTATATTAGATTGGTCA
GATCCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAAGA
TTCCAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCTA
GTACCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAAT
ACTTTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATT
TAAAGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGATCA
ATAGTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTCA
CTGATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAG
ATTTCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAACTGATCAAAAGA
GAATGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAAAATCATTTAGCAATTA
AAAAGATTCAAAATTTCCAACAAGAATAGAATTATTTAAATATTCTTTCNCGGGTTCAAG
A
Length of 3' end seq. 781
Connected seq. ID VHB821P
Connected seq. 
>VHB821P.Seq
AATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTC
AAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGC
AACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAG
AAGAAGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAA
TTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAA
TTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAG
AAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAAT
CATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTAT
CAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGA
TCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTG
TCTATTATTCAATGATTGATATATTTT----------TAGAGAGAATAAAGAACAATTGA
ATAATATGTACCNTTCAGAGTTGGTGACAGAGTTGGAAAGAGACATTAGACAATTGGGTG
TCTCCGAGACTGGTTTAACCTTTTTAGATCATTTTAGACTTTTAATCACTCATATTGGTA
ATGCTATGGGTTATATTAGATTGGTCAGATCCGGTGGTTTACACTATTGTAGTAATGCTA
TCAAATTCGTACCAGACTTGAAAAAGATTCCAAAATTCCAAGATTTAACCTCAAAAGATG
CCCTCTCACCTGAAACAATTCAAGCTAGTACCAATTTGGATTCAATCATTCATAATCTTT
CAAATAATTTATCAGAGGGTACTGAATACTTTAAGATGTTGGTAAACGTTTTCGCTACAG
AGTTTCGAAATATCGCTAATCAACATTTAAAGAATTTCTATATCATCGTACCTGCCTTGG
CCTCAAATTACATCGATCATATGATCAATAGTAAAGATAAACTCTTTAAGAAATCAAAAG
CAATCGGTGCTGAAGCTTTATTATTCACTGATGATGGCTTTGCAATTGGTTTGGCTTATA
TCTTAAAATTATTAGATCAAAATAAAGATTTCGATTCTCTTCATTGGTTCGATCGTATTC
AATTGAAATGTGAAACTGATCAAAAGAGAATGTTGGCTGAAGCAAATGCAAAAGGTATCA
AAGAGGATCAAAATCATTTAGCAATTAAAAAGATTCAAAATTTCCAACAAGAATAGAATT
ATTTAAATATTCTTTCNCGGGTTCAAGA
Length of connected seq. 1398
Full length Seq ID -
Full length Seq. -
Length of full length seq. -