>VHB812 (VHB812Q) /CSM/VH/VHB8-A/VHB812Q.Seq.d/
XXXXXXXXXXAAATACCGTTAGAGAAATCATTGGTCAATCAATTGAAAATCCAATTGTTT
TAATTGCTGGTTTAGCAAATACTTATTCAGGTTATATTGCAACATTTGAAGAGTTTCAAG
TTCAAAGATATGAAGGTGCATCAACCGTTTTTGGACCTCATACTTTAGGAGCATATCAAC
AAGAATTTGCAAATCTTGCACAATCAATTGTAGATGGTAGTCAAGCTGATCCAGGAACAT
TCCCAAGAAATATGTCTGGTCATACACCATTCTTTTTACCACCAGTCATTGTTGATGTAG
CTCCAAAATTCGACGATTTCGGTGATATCTATACTGATGTCTCTACAACTACACCATATT
CAATCAATCAAACTGTTACCGTCATTTTCTATGGTGCCAATCTTAGAAATAATTTCATGA
CTGAGTCTTCTTTCCTCACTGTTGATCAATTACAATCTAATGGTCAATGGACTACAATTT
TAAATGATGGTGATTGGGATACAAAATTATATTGGAAAATGCATGATTTAGGTTTTTCAC
TCATCACTGTTGATTGGACAATCTCTCCAATCACTCAACCAGGTACTTATAGAATAACTC
ATAGTGGTTATGCTAAGAAAAATCCATTCTCTGATAATTTAACTTTTTATCAAGGTATTT
CAAG

---NTVREIIGQSIENPIVLIAGLANTYSGYIATFEEFQVQRYEGASTVFGPHTLGAYQQ
EFANLAQSIVDGSQADPGTFPRNMSGHTPFFLPPVIVDVAPKFDDFGDIYTDVSTTTPYS
INQTVTVIFYGANLRNNFMTESSFLTVDQLQSNGQWTTILNDGDWDTKLYWKMHDLGFSL
ITVDWTISPITQPGTYRITHSGYAKKNPFSDNLTFYQGIS

Frame A:
---NTVREIIGQSIENPIVLIAGLANTYSGYIATFEEFQVQRYEGASTVFGPHTLGAYQQ
EFANLAQSIVDGSQADPGTFPRNMSGHTPFFLPPVIVDVAPKFDDFGDIYTDVSTTTPYS
INQTVTVIFYGANLRNNFMTESSFLTVDQLQSNGQWTTILNDGDWDTKLYWKMHDLGFSL
ITVDWTISPITQPGTYRITHSGYAKKNPFSDNLTFYQGIS


Frame B:
---iplekslvnqlkiqlf*llv*qiliqvilqhlksfkfkdmkvhqpfldlil*ehink
nlqilhnql*mvvkliqehsqeiclvihhsfyhqsllm*lqnstisvisilmslqlhhiq
sikllpsfsmvpileiis*lsllssllinynlmvnglqf*mmvigiqnyigkcmi*vfhs
slligqslqslnqvlie*livvmlrkihslii*lfikvfq


Frame C:
---yr*rnhwsin*ksncfncwfskylfrlycni*rvssski*rcinrfwtsyfrsistr
icksctincrw*ss*srnipkkyvwsytilfttshc*csskirrfr*yly*clynytifn
qsncyrhflwcqs*k*fhd*vffphc*siti*wsmdynfk*w*lgykiilena*frffth
hc*ldnlsnhstryl*nns*wlc*eksil**fnflsryfk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB812 (VHB812Q) /CSM/VH/VHB8-A/VHB812Q.Seq.d/                       1296   0.0
VHP543 (VHP543Q) /CSM/VH/VHP5-B/VHP543Q.Seq.d/                       1289   0.0
VHO854 (VHO854Q) /CSM/VH/VHO8-C/VHO854Q.Seq.d/                       1289   0.0
VHI431 (VHI431Q) /CSM/VH/VHI4-B/VHI431Q.Seq.d/                       1289   0.0
VHI396 (VHI396Q) /CSM/VH/VHI3-D/VHI396Q.Seq.d/                       1289   0.0
VHD809 (VHD809Q) /CSM/VH/VHD8-A/VHD809Q.Seq.d/                       1289   0.0
VFM574 (VFM574Q) /CSM/VF/VFM5-D/VFM574Q.Seq.d/                       1289   0.0
SFK788 (SFK788Q) /CSM/SF/SFK7-D/SFK788Q.Seq.d/                       1289   0.0
AHM465 (AHM465Q) /CSM/AH/AHM4-C/AHM465Q.Seq.d/                       1289   0.0
AHD485 (AHD485Q) /CSM/AH/AHD4-D/AHD485Q.Seq.d/                       1289   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U82513|U82513.1 Dictyostelium discoideum random slug cDNA25 protein (rsc25) mRNA, partial cds.  1289   0.0    1
AB121061|AB121061.1 Dictyostelium discoideum dcd2A mRNA for ceramidase, complete cds.  1289   0.0    1
BU801815|BU801815.1 SJF2DTD01 SJF Schistosoma japonicum cDNA, mRNA sequence.    48   0.32   1
BU718532|BU718532.1 SJM2CKE01 SJM Schistosoma japonicum cDNA, mRNA sequence.    48   0.32   1
BU718309|BU718309.1 SJM2CHH09 SJM Schistosoma japonicum cDNA, mRNA sequence.    48   0.32   1
AC165363|AC165363.1 Mus musculus chromosome 17 clone RP23-59N14, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    48   0.32   1
AC165167|AC165167.3 Mus musculus BAC clone RP23-27M6 from chromosome 17, complete sequence.    48   0.32   1
AC122257|AC122257.4 Mus musculus chromosome 17 clone RP23-178I11, complete sequence.    48   0.32   1
AF057019|AF057019.1 Dictyostelium discoideum interaptin (abpD) gene, complete cds.    34   0.44   4
BX322530|BX322530.5 Zebrafish DNA sequence from clone DKEY-19N13 in linkage group 16.    32   2.4    2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U82513_1(U82513|pid:none) Dictyostelium discoideum random slug c...   449   e-125
(Q55G11) RecName: Full=Neutral ceramidase B;          Short=N-CD...   337   2e-91
AC022464_28(AC022464|pid:none) Genomic sequence for Arabidopsis ...   157   3e-37
AK301784_1(AK301784|pid:none) Homo sapiens cDNA FLJ51366 complet...   150   2e-35
AL450382_3(AL450382|pid:none) Human DNA sequence from clone RP11...   150   2e-35
BC107105_1(BC107105|pid:none) Homo sapiens N-acylsphingosine ami...   150   2e-35
CP000777_2353(CP000777|pid:none) Leptospira biflexa serovar Pato...   150   4e-35
AL954360_4(AL954360|pid:none) Human DNA sequence from clone RP11...   150   4e-35
AP002819_24(AP002819|pid:none) Oryza sativa Japonica Group genom...   148   1e-34
EU200974_1(EU200974|pid:none) Triticum aestivum neutral ceramida...   148   1e-34

 psg: 0.69  gvh: 0.55  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VHB812 is cyt

>VHB812Z.Seq
NNNNNNNNNNAAATACCGTTAGAGAAATCATTGGTCAATCAATTGAAAATCCAATTGTTT
TAATTGCTGGTTTAGCAAATACTTATTCAGGTTATATTGCAACATTTGAAGAGTTTCAAG
TTCAAAGATATGAAGGTGCATCAACCGTTTTTGGACCTCATACTTTAGGAGCATATCAAC
AAGAATTTGCAAATCTTGCACAATCAATTGTAGATGGTAGTCAAGCTGATCCAGGAACAT
TCCCAAGAAATATGTCTGGTCATACACCATTCTTTTTACCACCAGTCATTGTTGATGTAG
CTCCAAAATTCGACGATTTCGGTGATATCTATACTGATGTCTCTACAACTACACCATATT
CAATCAATCAAACTGTTACCGTCATTTTCTATGGTGCCAATCTTAGAAATAATTTCATGA
CTGAGTCTTCTTTCCTCACTGTTGATCAATTACAATCTAATGGTCAATGGACTACAATTT
TAAATGATGGTGATTGGGATACAAAATTATATTGGAAAATGCATGATTTAGGTTTTTCAC
TCATCACTGTTGATTGGACAATCTCTCCAATCACTCAACCAGGTACTTATAGAATAACTC
ATAGTGGTTATGCTAAGAAAAATCCATTCTCTGATAATTTAACTTTTTATCAAGGTATTT
CAAG