>VHB802 (VHB802Q) /CSM/VH/VHB8-A/VHB802Q.Seq.d/
TTCATTTTTACCACCACAACACCAACACCACCACCACCATTCAAATTTATCACAATCATT
ATCAATTAATAATTTAGCACATCGTTTACCAACTTCATTATCATCATCATCACTATCATC
AAATAATACCTATGATAGTTTACAATTCCAACAACAACAACAACACCATCACCACAATCA
TAATAATCATCAAAATTCAAATCAACCATTAGCAACAATTGTACCATCATTTTCAAAATT
TAAAAACTCATTTGATCAATTATTAGAAGAACAAGATTTTAGTAAAAAGAAAAGAGAAAC
ACTTCAAAAATTATCAAAAGAACAAAAATGGAATTTATTACTTCAATATAGAAATTCAAC
TTTAAATATGTTAAATATGAAAGAGCATTCAAATAATAGTAATAATAATCAATCAATTAA
AAATTCAACTATAATAGCATCAACAACAACAACAACAACAACAAATGGTGAATCATTGGA
TGGTTCATCAATTCAACAACAAATTACAAAAAATTCAAATTTTCAATCATCAAGATTTTA
TATTGATAATTTAACAAGTAAAACAGATATTTCATTAGTAAAAGAAGTTCATGACTTTTT
AAAAGAAAGTGGACCAGAGGTATCAATATTTATTGGTATGGGTGGTATTGAAAGTTTATT
AGGAATTTTACAATTTAATTTAGATAATGCGCAATTACAATTTGATGAAXXXXXXXXXXA
ATACTTCCAATTGTAATTGGTGAATTACCAAAATGTAAAATCCCAAAAATCATTGACTCN
TATGTAACATTTGCCGATATGGATGAGATTGAAATAGCAAGACAATTATCACTCCTCACA
TTCCCTTTATATTCTAGAATTGAAACTTCGGAACTTTTAAATCAACGTTGGTCAAAACCA
GGTGGTGGTCCAAATATTATGTCTGTCGTTGGTTTATTTAATAAAATTTCAAATTGGGTT
TCATTTACAATTGTTAATCAACCAAAACTTAGAGATAGAGCCGTTGTCTATGGTAAAATG
GTTAAAATTGCCAATGCTTTCTATGAACTTCGTAATTATCATCTCTTAATGGCAATTATT
TCCGGTTTAAATGCTTCTCCTGTACTTAGATTAAAATATACCAAAGGTAAACTTTCAAAA
AATCTTAAAGATAATCTTGATACCTTGGAAGAGTTAATGTCAACTCAATCCTCAATGAAA
AATTATAGAGCAGATTTAGCTGCTGCTAGTCCTCCAGCAATTCCATTTATGGGCTTTCAT
TTATCTGATCTAGTTTTCATCGATGAAGGTAATCAACAATTATGTGACTCTAGAATTAAT
TTCAAAAAATTGGAAATGTATAAAAAAACAATTGCAACACTTCAAAATTTCTCACTTTTC
CCTTATCAATTCACTCCTGTTCCAATCATTCAAAATTACTTTTTAGATTATAAAATTGTT
GCGATAAACCGATTTAGAAAGAGTTTATCTGCNAACCAAGAAATGCGAAAGATA

SFLPPQHQHHHHHSNLSQSLSINNLAHRLPTSLSSSSLSSNNTYDSLQFQQQQQHHHHNH
NNHQNSNQPLATIVPSFSKFKNSFDQLLEEQDFSKKKRETLQKLSKEQKWNLLLQYRNST
LNMLNMKEHSNNSNNNQSIKNSTIIASTTTTTTTNGESLDGSSIQQQITKNSNFQSSRFY
IDNLTSKTDISLVKEVHDFLKESGPEVSIFIGMGGIESLLGILQFNLDNAQLQFDE---

---ILPIVIGELPKCKIPKIIDSYVTFADMDEIEIARQLSLLTFPLYSRIETSELLNQRW
SKPGGGPNIMSVVGLFNKISNWVSFTIVNQPKLRDRAVVYGKMVKIANAFYELRNYHLLM
AIISGLNASPVLRLKYTKGKLSKNLKDNLDTLEELMSTQSSMKNYRADLAAASPPAIPFM
GFHLSDLVFIDEGNQQLCDSRINFKKLEMYKKTIATLQNFSLFPYQFTPVPIIQNYFLDY
KIVAINRFRKSLSANQEMRKI

Frame A:
fifttttptppppfkfitiiin**fstsftnfiiiiitiik*yl**ftiptttttpspqs
**sskfkstisnnctiifki*kli*siirrtrf**kekrntskiikrtkmefitsi*kfn
fkyvkyerafk*****sin*kfnynsinnnnnnnkw*iigwfinsttnykkfkfsiikil
y**fnk*nryfiskrss*lfkrkwtrginiywygwy*kfirnfti*fr*caiti**---

---ntsncnw*itkm*npknh*lxcnicryg*d*nsktiitphipfif*n*nfgtfkstl
vktrwwskyyvcrwfi**nfklgfiync*stkt*r*srclw*ng*ncqcfl*ts*lssln
gnyfrfkcfsct*ikiyqr*tfkks*r*s*ylgrvnvnsilnekl*srfscc*sssnsiy
glsfi*ssfhr*r*stim*l*n*fqkignv*knncntskfltfplsihscsnhskllfrl
*nccdkpi*keficxprnakd

Frame B:
SFLPPQHQHHHHHSNLSQSLSINNLAHRLPTSLSSSSLSSNNTYDSLQFQQQQQHHHHNH
NNHQNSNQPLATIVPSFSKFKNSFDQLLEEQDFSKKKRETLQKLSKEQKWNLLLQYRNST
LNMLNMKEHSNNSNNNQSIKNSTIIASTTTTTTTNGESLDGSSIQQQITKNSNFQSSRFY
IDNLTSKTDISLVKEVHDFLKESGPEVSIFIGMGGIESLLGILQFNLDNAQLQFDE---

---ILPIVIGELPKCKIPKIIDSYVTFADMDEIEIARQLSLLTFPLYSRIETSELLNQRW
SKPGGGPNIMSVVGLFNKISNWVSFTIVNQPKLRDRAVVYGKMVKIANAFYELRNYHLLM
AIISGLNASPVLRLKYTKGKLSKNLKDNLDTLEELMSTQSSMKNYRADLAAASPPAIPFM
GFHLSDLVFIDEGNQQLCDSRINFKKLEMYKKTIATLQNFSLFPYQFTPVPIIQNYFLDY
KIVAINRFRKSLSANQEMRKI

Frame C:
hfyhhntnttttiqiyhnhyqlii*hivyqlhyhhhhyhqiipmivynsnnnnntittii
iiikiqinh*qqlyhhfqnlkthliny*knkilvkrkekhfknyqknkngiyyfnieiql
*ic*i*ksiqiiviiinqlkiql**hqqqqqqqqmvnhwmvhqfnnklqkiqifnhqdfi
lii*qvkqifh**kkfmtf*kkvdqryqyllvwvvlkvy*efynli*imrnynlm---

---yfql*lvnyqnvksqksltxm*hlpiwmrlk*qdnyhsshslyilelklrnf*invg
qnqvvvqilclslvylikfqigfhlqllinqnleieplsmvkwlklpmlsmnfviiis*w
qlfpv*mlllyld*nipkvnfqkilkiilipwks*cqlnpq*kiieqi*lllvlqqfhlw
afiyli*fssmkvinnyvtlelisknwkcikkqlqhfkishfslinsllfqsfkitf*ii
kllr*tdlervylxtkkcer

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB802 (VHB802Q) /CSM/VH/VHB8-A/VHB802Q.Seq.d/                       1929   0.0
AHH867 (AHH867Q) /CSM/AH/AHH8-C/AHH867Q.Seq.d/                       1352   0.0
AHF363 (AHF363Q) /CSM/AH/AHF3-C/AHF363Q.Seq.d/                       1352   0.0
SHD588 (SHD588Q) /CSM/SH/SHD5-D/SHD588Q.Seq.d/                       1332   0.0
VHF325 (VHF325Q) /CSM/VH/VHF3-B/VHF325Q.Seq.d/                       1138   0.0
AFF729 (AFF729Q) /CSM/AF/AFF7-B/AFF729Q.Seq.d/                       1066   0.0
SHE545 (SHE545Q) /CSM/SH/SHE5-B/SHE545Q.Seq.d/                       1023   0.0
CHJ817 (CHJ817Q) /CSM/CH/CHJ8-A/CHJ817Q.Seq.d/                        817   0.0
SHG608 (SHG608Q) /CSM/SH/SHG6-A/SHG608Q.Seq.d/                         46   0.002
SHB527 (SHB527Q) /CSM/SH/SHB5-B/SHB527Q.Seq.d/                         46   0.002

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117080|AC117080.2 Dictyostelium discoideum chromosome 2 map complement(821514-735598) strain AX4, complete sequence.    42   2e-05  8
AC174086|AC174086.2 Strongylocentrotus purpuratus clone R3-56D18, WORKING DRAFT SEQUENCE, 7 unordered pieces.    46   2e-04  2
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    38   9e-04  11
AC166729|AC166729.3 Bos taurus clone CH240-180P18, *** SEQUENCING IN PROGRESS ***, 16 unordered pieces.    56   0.003  1
AC084259|AC084259.1 Homo sapiens chromosome 8 clone RP5-301D7 map 8, LOW-PASS SEQUENCE SAMPLING.    54   0.006  4
AE014846|AE014846.1 Plasmodium falciparum 3D7 chromosome 12, section 3 of 9 of the complete sequence.    42   0.008  2
AC005506|AC005506.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    42   0.008  2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    50   0.010  15
CX576665|CX576665.1 TTE00034246 Amplicon Express - Conjugative Form Tetrahymena thermophila cDNA, mRNA sequence.    44   0.010  2
AY160102|AY160102.1 Dictyostelium discoideum nucleotide exchange factor RasGEF M (gefM) gene, complete cds.    38   0.011  5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY160098_1(AY160098|pid:none) Dictyostelium discoideum nucleotid...   175   4e-42
AY160099_1(AY160099|pid:none) Dictyostelium discoideum nucleotid...   172   2e-41
AY160100_1(AY160100|pid:none) Dictyostelium discoideum nucleotid...   171   6e-41
AF474377_1(AF474377|pid:none) Dictyostelium discoideum Ras GTP e...   171   6e-41
U53884_1(U53884|pid:none) Dictyostelium discoideum aimless RasGE...   164   6e-39
AY160090_1(AY160090|pid:none) Dictyostelium discoideum nucleotid...   164   6e-39
AF275723_1(AF275723|pid:none) Dictyostelium discoideum Ras guani...   157   7e-37
AC116987_20(AC116987|pid:none) Dictyostelium discoideum chromoso...   150   2e-34
AC115592_17(AC115592|pid:none) Dictyostelium discoideum chromoso...   147   1e-33
AC115682_8(AC115682|pid:none) Dictyostelium discoideum chromosom...   141   7e-32

 psg: 0.77  gvh: 0.48  alm: 0.31  top: 0.50  tms: 0.07  mit: 0.45  mip: 0.06
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.05  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: mitochondrial
	 20.0 %: nuclear
	 16.0 %: endoplasmic reticulum
	 12.0 %: Golgi
	  8.0 %: cytoplasmic
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VHB802 is mit

>VHB802F.Seq
TTCATTTTTACCACCACAACACCAACACCACCACCACCATTCAAATTTATCACAATCATT
ATCAATTAATAATTTAGCACATCGTTTACCAACTTCATTATCATCATCATCACTATCATC
AAATAATACCTATGATAGTTTACAATTCCAACAACAACAACAACACCATCACCACAATCA
TAATAATCATCAAAATTCAAATCAACCATTAGCAACAATTGTACCATCATTTTCAAAATT
TAAAAACTCATTTGATCAATTATTAGAAGAACAAGATTTTAGTAAAAAGAAAAGAGAAAC
ACTTCAAAAATTATCAAAAGAACAAAAATGGAATTTATTACTTCAATATAGAAATTCAAC
TTTAAATATGTTAAATATGAAAGAGCATTCAAATAATAGTAATAATAATCAATCAATTAA
AAATTCAACTATAATAGCATCAACAACAACAACAACAACAACAAATGGTGAATCATTGGA
TGGTTCATCAATTCAACAACAAATTACAAAAAATTCAAATTTTCAATCATCAAGATTTTA
TATTGATAATTTAACAAGTAAAACAGATATTTCATTAGTAAAAGAAGTTCATGACTTTTT
AAAAGAAAGTGGACCAGAGGTATCAATATTTATTGGTATGGGTGGTATTGAAAGTTTATT
AGGAATTTTACAATTTAATTTAGATAATGCGCAATTACAATTTGATGAANNNNNNNNNN

>VHB802Z.Seq
NNNNNNNNNNAATACTTCCAATTGTAATTGGTGAATTACCAAAATGTAAAATCCCAAAAA
TCATTGACTCNTATGTAACATTTGCCGATATGGATGAGATTGAAATAGCAAGACAATTAT
CACTCCTCACATTCCCTTTATATTCTAGAATTGAAACTTCGGAACTTTTAAATCAACGTT
GGTCAAAACCAGGTGGTGGTCCAAATATTATGTCTGTCGTTGGTTTATTTAATAAAATTT
CAAATTGGGTTTCATTTACAATTGTTAATCAACCAAAACTTAGAGATAGAGCCGTTGTCT
ATGGTAAAATGGTTAAAATTGCCAATGCTTTCTATGAACTTCGTAATTATCATCTCTTAA
TGGCAATTATTTCCGGTTTAAATGCTTCTCCTGTACTTAGATTAAAATATACCAAAGGTA
AACTTTCAAAAAATCTTAAAGATAATCTTGATACCTTGGAAGAGTTAATGTCAACTCAAT
CCTCAATGAAAAATTATAGAGCAGATTTAGCTGCTGCTAGTCCTCCAGCAATTCCATTTA
TGGGCTTTCATTTATCTGATCTAGTTTTCATCGATGAAGGTAATCAACAATTATGTGACT
CTAGAATTAATTTCAAAAAATTGGAAATGTATAAAAAAACAATTGCAACACTTCAAAATT
TCTCACTTTTCCCTTATCAATTCACTCCTGTTCCAATCATTCAAAATTACTTTTTAGATT
ATAAAATTGTTGCGATAAACCGATTTAGAAAGAGTTTATCTGCNAACCAAGAAATGCGAA
AGATA

>VHB802P.Seq
TTCATTTTTACCACCACAACACCAACACCACCACCACCATTCAAATTTATCACAATCATT
ATCAATTAATAATTTAGCACATCGTTTACCAACTTCATTATCATCATCATCACTATCATC
AAATAATACCTATGATAGTTTACAATTCCAACAACAACAACAACACCATCACCACAATCA
TAATAATCATCAAAATTCAAATCAACCATTAGCAACAATTGTACCATCATTTTCAAAATT
TAAAAACTCATTTGATCAATTATTAGAAGAACAAGATTTTAGTAAAAAGAAAAGAGAAAC
ACTTCAAAAATTATCAAAAGAACAAAAATGGAATTTATTACTTCAATATAGAAATTCAAC
TTTAAATATGTTAAATATGAAAGAGCATTCAAATAATAGTAATAATAATCAATCAATTAA
AAATTCAACTATAATAGCATCAACAACAACAACAACAACAACAAATGGTGAATCATTGGA
TGGTTCATCAATTCAACAACAAATTACAAAAAATTCAAATTTTCAATCATCAAGATTTTA
TATTGATAATTTAACAAGTAAAACAGATATTTCATTAGTAAAAGAAGTTCATGACTTTTT
AAAAGAAAGTGGACCAGAGGTATCAATATTTATTGGTATGGGTGGTATTGAAAGTTTATT
AGGAATTTTACAATTTAATTTAGATAATGCGCAATTACAATTTGATGAA----------A
ATACTTCCAATTGTAATTGGTGAATTACCAAAATGTAAAATCCCAAAAATCATTGACTCN
TATGTAACATTTGCCGATATGGATGAGATTGAAATAGCAAGACAATTATCACTCCTCACA
TTCCCTTTATATTCTAGAATTGAAACTTCGGAACTTTTAAATCAACGTTGGTCAAAACCA
GGTGGTGGTCCAAATATTATGTCTGTCGTTGGTTTATTTAATAAAATTTCAAATTGGGTT
TCATTTACAATTGTTAATCAACCAAAACTTAGAGATAGAGCCGTTGTCTATGGTAAAATG
GTTAAAATTGCCAATGCTTTCTATGAACTTCGTAATTATCATCTCTTAATGGCAATTATT
TCCGGTTTAAATGCTTCTCCTGTACTTAGATTAAAATATACCAAAGGTAAACTTTCAAAA
AATCTTAAAGATAATCTTGATACCTTGGAAGAGTTAATGTCAACTCAATCCTCAATGAAA
AATTATAGAGCAGATTTAGCTGCTGCTAGTCCTCCAGCAATTCCATTTATGGGCTTTCAT
TTATCTGATCTAGTTTTCATCGATGAAGGTAATCAACAATTATGTGACTCTAGAATTAAT
TTCAAAAAATTGGAAATGTATAAAAAAACAATTGCAACACTTCAAAATTTCTCACTTTTC
CCTTATCAATTCACTCCTGTTCCAATCATTCAAAATTACTTTTTAGATTATAAAATTGTT
GCGATAAACCGATTTAGAAAGAGTTTATCTGCNAACCAAGAAATGCGAAAGATA