>VHB795 (VHB795Q) /CSM/VH/VHB7-D/VHB795Q.Seq.d/
ACTTCACATTTAGGTCCTATATTCTAATTCATAATTAATTTTATTTGTTTTTTTTTGTAG
AACATAAAAAAATGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATC
AGCTTATGTCCCTTATTATTAATACTTTTTATTCAAATAAAGAAGTATTTTTAAGAGAAT
TGATTTCAAATGCATCTGATGCCTTAGATAAAATTCGTTACCAATCATTAACTGATGCAT
CAGTTTTGGAATCAAAAACTGAACTCGAAATTAAAATTATTCCAGATAAGACTGCCAAAA
CTCTTACACTCATTGATAGTGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAG
GTACTATTGCTCGTTCAGGTACTAAAAACTTTATGGAACAATTACAAAGTGGTGCCGCTG
ATATCAGTATGATTGGTCAATTTGGTGTTGGTTTCTACTCTGCTTACTTAGTTACCGATA
CTGTTATTGTTCACTCTAAAAACAATGATGATGAACAATACGTTTGGGAATCAAGTGCTG
GTGGTGAATTTACCATTGCTCTCGATCATACCGAAXXXXXXXXXXAAAAAAGCTGTTGAA
AACTCTCCATTCATTGAAGGTCTCAAGAAGAAGAATCTTGAAGTCATTTACATGTGTGAC
CCAATTGATGAATATGCTGTTCAACAATTAAAAGAATATGACGGTAAGAAATTAGTATCA
ATTACCAAAGAAGGTTTGAAATTAGATGAAACCGAAGATGAAAAGAAAAAAGCTGAACAA
GATAAAGCTGCCAATGAAGAATTACTCAAACAAGTTAAAGATGTATTGGGTGATAAAGTT
GAAAAGGTAGTTCTCTCAACTCGTCTTGCCAACTCACCATGTGTTTTAGTTACCTCTGAA
TATGGTTGGTCAGCAAACATGGAACGTATCATGAAAGCTCAAGCTCTCCGTGACTCTTCA
ATGTCATCATACATGTCATCAAAGAAAACTTTAGAATTAAATCCAGATCACCCAATCGTT
AGAGATTTAGCCAAAAAAGCCGCCGAAAAAAGTAAAACTTTCAAAGACTTTGTTTACTTA
TTATATGAAACTGCTCTCTTAACTTCAGGTTTCTCATTAGATGAACCATCATCATTTGCT
TCACGTATTCATCGTATGATTAAATTAGGTTTAAGCATTCAAGATGATTCATCAGCAACA
ACTGAAGAATCAACAAACACAACAACCAGTGATGATATTCCACCATTAGAAGAAAACGAT
GAACCATCTGAAATGGAAAAAGTCGACTAGATTTAATTACTAGCCCTTTTGNTATCAATT
TTTTGGAATATTGTTATATTATTATAATAAAATAAACTTTAAAAAAAATCTTC

ftfrsyilihn*FYLFFFVEHKKMAESQVERFTFQAEINQLMSLIINTFYSNKEVFLREL
ISNASDALDKIRYQSLTDASVLESKTELEIKIIPDKTAKTLTLIDSGIGMTKTDMVKNLG
TIARSGTKNFMEQLQSGAADISMIGQFGVGFYSAYLVTDTVIVHSKNNDDEQYVWESSAG
GEFTIALDHTE---

---KKAVENSPFIEGLKKKNLEVIYMCDPIDEYAVQQLKEYDGKKLVSITKEGLKLDETE
DEKKKAEQDKAANEELLKQVKDVLGDKVEKVVLSTRLANSPCVLVTSEYGWSANMERIMK
AQALRDSSMSSYMSSKKTLELNPDHPIVRDLAKKAAEKSKTFKDFVYLLYETALLTSGFS
LDEPSSFASRIHRMIKLGLSIQDDSSATTEESTNTTTSDDIPPLEENDEPSEMEKVD*i*
llallxsifwnivill**nkl*kks

Frame A:
tshlgpif*fiinficffl*nikkwlnhklnvshsklklislcplllilfiqikkyf*en
*fqmhlmp*ikfvtnh*lmhqfwnqklnsklklfqirlpkllhslivvlv*pkpiw*ri*
vlllvqvlktlwnnykvvplisv*lvnlvlvstllt*lpilllftlktmmmnntfgnqvl
vvnlpllsiip---

---KKAVENSPFIEGLKKKNLEVIYMCDPIDEYAVQQLKEYDGKKLVSITKEGLKLDETE
DEKKKAEQDKAANEELLKQVKDVLGDKVEKVVLSTRLANSPCVLVTSEYGWSANMERIMK
AQALRDSSMSSYMSSKKTLELNPDHPIVRDLAKKAAEKSKTFKDFVYLLYETALLTSGFS
LDEPSSFASRIHRMIKLGLSIQDDSSATTEESTNTTTSDDIPPLEENDEPSEMEKVD*i*
llallxsifwnivill**nkl*kks

Frame B:
lhi*vlysns*lilfvffcrt*kng*its*tfhips*n*sayvpyy*yflfk*rsifkri
dfkci*clr*nslpiin*cisfgikn*trn*nysr*dcqnsyth**wywydqnrygkefr
yycsfry*klygtitkwcr*yqydwsiwcwfllcllsyrycycsl*kq***tirlgikcw
w*iyhcsrsyr---

---kkllktlhslkvsrrrilksftcvtqlmnmlfnn*knmtvrn*yqlpkkv*n*mkpk
mkrkklnkiklpmknysnklkmywviklkr*fsqlvlpthhvf*lplnmvgqqtwnvs*k
lklsvtlqchhtchqrkl*n*iqitqslei*pkkppkkvklsktlftyymklls*lqvsh
*mnhhhllhvfiv*ln*v*afkmihqqqlknqqtqqpvmifhh*kktmnhlkwkkstrfn
y*pfxyqffgillyyynkinfkknl

Frame C:
ftfrsyilihn*FYLFFFVEHKKMAESQVERFTFQAEINQLMSLIINTFYSNKEVFLREL
ISNASDALDKIRYQSLTDASVLESKTELEIKIIPDKTAKTLTLIDSGIGMTKTDMVKNLG
TIARSGTKNFMEQLQSGAADISMIGQFGVGFYSAYLVTDTVIVHSKNNDDEQYVWESSAG
GEFTIALDHTE---

---ksc*klsih*rsqeees*shlhv*pn**iccstikri*r*eisinyqrrfeir*nrr
*keks*tr*scq*ritqts*rcig**s*kgsslnsscqltmcfsyl*iwlvskhgtyhes
sssp*lfnviihvikenfriksrspnr*rfsqksrrkk*nfqrlcllii*ncslnfrfli
r*tiiicftyssyd*irfkhsr*fisnn*rinkhnnq**ystirrkr*ti*ngksrldli
tspfxinfleycyiiiik*tlkkif

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB795 (VHB795Q) /CSM/VH/VHB7-D/VHB795Q.Seq.d/                       1891   0.0
VHP152 (VHP152Q) /CSM/VH/VHP1-C/VHP152Q.Seq.d/                       1037   0.0
VHM703 (VHM703Q) /CSM/VH/VHM7-A/VHM703Q.Seq.d/                       1037   0.0
VHK340 (VHK340Q) /CSM/VH/VHK3-B/VHK340Q.Seq.d/                       1037   0.0
VHK280 (VHK280Q) /CSM/VH/VHK2-D/VHK280Q.Seq.d/                       1037   0.0
VHI607 (VHI607Q) /CSM/VH/VHI6-A/VHI607Q.Seq.d/                       1037   0.0
VHQ442 (VHQ442Q) /CSM/VH/VHQ4-B/VHQ442Q.Seq.d/                       1035   0.0
VHP202 (VHP202Q) /CSM/VH/VHP2-A/VHP202Q.Seq.d/                       1027   0.0
VHL421 (VHL421Q) /CSM/VH/VHL4-A/VHL421Q.Seq.d/                       1027   0.0
VHK487 (VHK487Q) /CSM/VH/VHK4-D/VHK487Q.Seq.d/                       1027   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L43591|L43591.1 Dictyostelium discoideum (clone C 6.1.1) heat shock cognate protein (hsc90) mRNA, complete cds.   989   0.0    2
L34027|L34027.1 Plasmodium falciparum (clone Dd2) heat shock protein 86 gene, complete cds.    90   4e-51  9
L34028|L34028.1 Plasmodium falciparum (clone HB3) heat shock protein 86 gene, complete cds.    90   4e-51  8
Z29667|Z29667.1 P.falciparum (7) mRNA for heat-shock protein.      90   6e-49  8
AY122634|AY122634.1 Naegleria gruberi strain ATCC 30224 heat shock protein 90 (HSP-90) gene, partial cds.    82   6e-49  8
AY729861|AY729861.1 Plectospira myriandra heat shock protein 90 gene, partial cds.    74   1e-47  7
AJ890081|AJ890081.1 Delia antiqua mRNA for Hsp90 protein.          68   2e-47  8
AY729852|AY729852.1 Apodachlya brachynema heat shock protein 90 gene, partial cds.    70   1e-46  8
AF249998|AF249998.1 Habrotrocha constricta 82 kDa heat shock protein 2 (hsp82-2) gene, partial cds.    88   2e-46  4
AF030694|AF030694.2 Plasmodium falciparum strain Dd2 heat shock protein 86 (HSP86), O1 (o1), O3 (o3), O2 (o2), CG8 (cg8), CG4 (cg4), CG3 (cg3), putative chloroquine resistance transporter (crt), CG9 (cg9), CG1 (cg1), CG6 (cg6), CG2 (cg2), and CG7 (cg7) genes, complete cds.    90   2e-46  10

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54651) RecName: Full=Heat shock cognate 90 kDa protein;             338   3e-91
L43591_1(L43591|pid:none) Dictyostelium discoideum (clone C 6.1....   338   3e-91
AB300196_1(AB300196|pid:none) Physarum polycephalum hsp90 mRNA f...   258   3e-67
FJ392027_1(FJ392027|pid:none) Portunus trituberculatus heat shoc...   249   1e-64
EF687776_1(EF687776|pid:none) Crassostrea gigas heat shock prote...   249   2e-64
(P04810) RecName: Full=Heat shock protein 83; AltName: Full=HSP ...   248   4e-64
AY851367_1(AY851367|pid:none) Liriomyza huidobrensis heat shock ...   248   4e-64
U57467_1(U57467|pid:none) Drosophila melanogaster heat shock pro...   248   4e-64
(P04811) RecName: Full=Heat shock protein 83; AltName: Full=HSP ...   248   4e-64
U57459_1(U57459|pid:none) Drosophila melanogaster heat shock pro...   248   4e-64

 psg: 0.50  gvh: 0.46  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 24.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHB795 is cyt

>VHB795F.Seq
ACTTCACATTTAGGTCCTATATTCTAATTCATAATTAATTTTATTTGTTTTTTTTTGTAG
AACATAAAAAAATGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATC
AGCTTATGTCCCTTATTATTAATACTTTTTATTCAAATAAAGAAGTATTTTTAAGAGAAT
TGATTTCAAATGCATCTGATGCCTTAGATAAAATTCGTTACCAATCATTAACTGATGCAT
CAGTTTTGGAATCAAAAACTGAACTCGAAATTAAAATTATTCCAGATAAGACTGCCAAAA
CTCTTACACTCATTGATAGTGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAG
GTACTATTGCTCGTTCAGGTACTAAAAACTTTATGGAACAATTACAAAGTGGTGCCGCTG
ATATCAGTATGATTGGTCAATTTGGTGTTGGTTTCTACTCTGCTTACTTAGTTACCGATA
CTGTTATTGTTCACTCTAAAAACAATGATGATGAACAATACGTTTGGGAATCAAGTGCTG
GTGGTGAATTTACCATTGCTCTCGATCATACCGAANNNNNNNNNN

>VHB795Z.Seq
NNNNNNNNNNAAAAAAGCTGTTGAAAACTCTCCATTCATTGAAGGTCTCAAGAAGAAGAA
TCTTGAAGTCATTTACATGTGTGACCCAATTGATGAATATGCTGTTCAACAATTAAAAGA
ATATGACGGTAAGAAATTAGTATCAATTACCAAAGAAGGTTTGAAATTAGATGAAACCGA
AGATGAAAAGAAAAAAGCTGAACAAGATAAAGCTGCCAATGAAGAATTACTCAAACAAGT
TAAAGATGTATTGGGTGATAAAGTTGAAAAGGTAGTTCTCTCAACTCGTCTTGCCAACTC
ACCATGTGTTTTAGTTACCTCTGAATATGGTTGGTCAGCAAACATGGAACGTATCATGAA
AGCTCAAGCTCTCCGTGACTCTTCAATGTCATCATACATGTCATCAAAGAAAACTTTAGA
ATTAAATCCAGATCACCCAATCGTTAGAGATTTAGCCAAAAAAGCCGCCGAAAAAAGTAA
AACTTTCAAAGACTTTGTTTACTTATTATATGAAACTGCTCTCTTAACTTCAGGTTTCTC
ATTAGATGAACCATCATCATTTGCTTCACGTATTCATCGTATGATTAAATTAGGTTTAAG
CATTCAAGATGATTCATCAGCAACAACTGAAGAATCAACAAACACAACAACCAGTGATGA
TATTCCACCATTAGAAGAAAACGATGAACCATCTGAAATGGAAAAAGTCGACTAGATTTA
ATTACTAGCCCTTTTGNTATCAATTTTTTGGAATATTGTTATATTATTATAATAAAATAA
ACTTTAAAAAAAATCTTC

>VHB795P.Seq
ACTTCACATTTAGGTCCTATATTCTAATTCATAATTAATTTTATTTGTTTTTTTTTGTAG
AACATAAAAAAATGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATC
AGCTTATGTCCCTTATTATTAATACTTTTTATTCAAATAAAGAAGTATTTTTAAGAGAAT
TGATTTCAAATGCATCTGATGCCTTAGATAAAATTCGTTACCAATCATTAACTGATGCAT
CAGTTTTGGAATCAAAAACTGAACTCGAAATTAAAATTATTCCAGATAAGACTGCCAAAA
CTCTTACACTCATTGATAGTGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAG
GTACTATTGCTCGTTCAGGTACTAAAAACTTTATGGAACAATTACAAAGTGGTGCCGCTG
ATATCAGTATGATTGGTCAATTTGGTGTTGGTTTCTACTCTGCTTACTTAGTTACCGATA
CTGTTATTGTTCACTCTAAAAACAATGATGATGAACAATACGTTTGGGAATCAAGTGCTG
GTGGTGAATTTACCATTGCTCTCGATCATACCGAA----------AAAAAAGCTGTTGAA
AACTCTCCATTCATTGAAGGTCTCAAGAAGAAGAATCTTGAAGTCATTTACATGTGTGAC
CCAATTGATGAATATGCTGTTCAACAATTAAAAGAATATGACGGTAAGAAATTAGTATCA
ATTACCAAAGAAGGTTTGAAATTAGATGAAACCGAAGATGAAAAGAAAAAAGCTGAACAA
GATAAAGCTGCCAATGAAGAATTACTCAAACAAGTTAAAGATGTATTGGGTGATAAAGTT
GAAAAGGTAGTTCTCTCAACTCGTCTTGCCAACTCACCATGTGTTTTAGTTACCTCTGAA
TATGGTTGGTCAGCAAACATGGAACGTATCATGAAAGCTCAAGCTCTCCGTGACTCTTCA
ATGTCATCATACATGTCATCAAAGAAAACTTTAGAATTAAATCCAGATCACCCAATCGTT
AGAGATTTAGCCAAAAAAGCCGCCGAAAAAAGTAAAACTTTCAAAGACTTTGTTTACTTA
TTATATGAAACTGCTCTCTTAACTTCAGGTTTCTCATTAGATGAACCATCATCATTTGCT
TCACGTATTCATCGTATGATTAAATTAGGTTTAAGCATTCAAGATGATTCATCAGCAACA
ACTGAAGAATCAACAAACACAACAACCAGTGATGATATTCCACCATTAGAAGAAAACGAT
GAACCATCTGAAATGGAAAAAGTCGACTAGATTTAATTACTAGCCCTTTTGNTATCAATT
TTTTGGAATATTGTTATATTATTATAATAAAATAAACTTTAAAAAAAATCTTC