>VHB771 (VHB771Q) /CSM/VH/VHB7-C/VHB771Q.Seq.d/
ATTATCTTAATCATCCAATTATTTTTTAAATATATATATAAATAAAATGAAAGTTTTATC
TGCCTTATGCGTTTTATTAGTCAGTGTTGCCACTGCCAAACAACAATTATCCGAAGTTGA
ATACAGAAATGCTTTCACCAATTGGATGATTGCTCACCAAAGACATTATTCATCAGAAGA
GTTCAATGGTCGTTACAATATCTTCAAAGCCAATATGGATTACGTCAATGAATGGAATAC
CAAAGGTAGTGAAACCGTTTTAGGTTTAAATGTCTTTGCTGATATCAGCAATGAAGAATA
CAGAGCCACCTATTTAGGTACCCCATTCGATGCTTCATCCCTCGAAATGACCGAATCCGA
TAAAATCTTCGATGCTTCCGCTCAAGTCGATTGGAGAACTCAAGGTGCTGTCACCCCAAT
CAAAAATCAAGGTCAATGTGGTGGTTGTTGGTCATTCTCAACTACTGGTGCCACTGAAGG
TGCTCAATACCTTGCCAACGGTAAAAAGAATTTAGTCTCATTATCCGAACAAAATTTAAT
CGATTGTTCAGGTTCATACGGTAACAACGGTTGTGAAGGTGGTTTAATGACCTTAGCTTT
CGAATACATCATCAXXXXXXXXXXATACGTTTCAGGTATCTACAATGAGCCAGCTTGCTC
TTCAACTCAATTAGATCACGGTGTCTTAGCTGTTGGTTTCGGTACTGGTAGTGGTTCATC
TGGTTCCCATGGTGGTTCCCAATCACAATCTGCTGGTTCAGATTCACAATCTGCTGGTTC
AGAATCATCCCAATCTGAATCCGGTTCACAATCACAATCTGAATCCGGTTCACAATCACA
ATCACAATCTGGTTCTCAATCATTCTCTGGTTCCTTATACTCTGGTTCATACTCTGGTTC
CCAATCTGGTTCCCAATCTGGTAACAGCGGTGCTGCTGTTAAACAAACTGGTGCTGGTTC
AGGTTCAGGTTCAGGTTCAGGTTCCGGTTCCGGTTCAGGTTCAGGTTCAGTCTCAGGTTC
AGCTTCAGGTTCCGCCTCAGGTTCCGCCTCAGGTTCATCAAGTGGTAGCAATAGCAACGG
TGGTGTTTATCCAACCGCTGGTGACTACTGGATCGTTAAAAACTCATGGGGTACCTCATG
GGGTATGGATGGTTACATTTTAATGACCAAAGGAAATAACAATCAATGCGGTATTGCAAC
AATGGCCTCACGTCCAACTGCTGTTGCATCACTTTAAATTAACGATACATATATATACAT
ATTTATATAATTTTATAATTAATAAAAAATAATACGAAAACATATAAAATTTATANNATA
AATAAATAAAAAATATT

ls*SSNYFLNIYINKMKVLSALCVLLVSVATAKQQLSEVEYRNAFTNWMIAHQRHYSSEE
FNGRYNIFKANMDYVNEWNTKGSETVLGLNVFADISNEEYRATYLGTPFDASSLEMTESD
KIFDASAQVDWRTQGAVTPIKNQGQCGGCWSFSTTGATEGAQYLANGKKNLVSLSEQNLI
DCSGSYGNNGCEGGLMTLAFEYII---

---YVSGIYNEPACSSTQLDHGVLAVGFGTGSGSSGSHGGSQSQSAGSDSQSAGSESSQS
ESGSQSQSESGSQSQSQSGSQSFSGSLYSGSYSGSQSGSQSGNSGAAVKQTGAGSGSGSG
SGSGSGSGSGSVSGSASGSASGSASGSSSGSNSNGGVYPTAGDYWIVKNSWGTSWGMDGY
ILMTKGNNNQCGIATMASRPTAVASL*indtyiyifi*fyn**kiirkhikfix*inkky

Frame A:
iiliiqlffkyiyk*nesficlmrfisqcchcqttiirs*iqkcfhqlddcspktlfirr
vqwslqylqsqyglrq*meyqr**nrfrfkclc*yqq*riqshlfrypircfiprndrir
*nlrcfrssrlensrcchpnqksrsmwwllvilnywch*rcsipcqr*kefsliirtkfn
rlfrfir*qrl*rwfndlsfrihh---

---irfrylq*asllfnsirsrclscwfryw*wfiwfpwwfpiticwfrfticwfriipi
*irftiti*irftititiwfsiilwflilwfilwfpiwfpiw*qrccc*tnwcwfrfrfr
frfrfrfrfrfslrfsfrfrlrfrlrfikw*q*qrwclsnrw*lldr*klmgylmgygwl
hfndqrk*qsmrycnngltsncccitln*ryiyihiyiil*liknntktykiyxink*ki

Frame B:
ls*SSNYFLNIYINKMKVLSALCVLLVSVATAKQQLSEVEYRNAFTNWMIAHQRHYSSEE
FNGRYNIFKANMDYVNEWNTKGSETVLGLNVFADISNEEYRATYLGTPFDASSLEMTESD
KIFDASAQVDWRTQGAVTPIKNQGQCGGCWSFSTTGATEGAQYLANGKKNLVSLSEQNLI
DCSGSYGNNGCEGGLMTLAFEYII---

---YVSGIYNEPACSSTQLDHGVLAVGFGTGSGSSGSHGGSQSQSAGSDSQSAGSESSQS
ESGSQSQSESGSQSQSQSGSQSFSGSLYSGSYSGSQSGSQSGNSGAAVKQTGAGSGSGSG
SGSGSGSGSGSVSGSASGSASGSASGSSSGSNSNGGVYPTAGDYWIVKNSWGTSWGMDGY
ILMTKGNNNQCGIATMASRPTAVASL*indtyiyifi*fyn**kiirkhikfix*inkky

Frame C:
ylnhpiif*iyi*ik*kfylpyafy*svlplpnnnypklntemlspig*lltkdiihqks
smvvtisskpiwitsmngipkvvkpf*v*msllisamknteppi*vphsmlhpsk*pnpi
kssmlplksigelkvlspqskikvnvvvvghsqllvplkvlntlptvkri*shypnki*s
ivqvhtvttvvkvv**p*lsntss---

---tfqvstmsqlalqln*itvs*llvsvlvvvhlvpmvvpnhnllvqihnllvqnhpnl
npvhnhnlnpvhnhnhnlvlnhslvpytlvhtlvpnlvpnlvtavlllnklvlvqvqvqv
qvpvpvqvqvqsqvqlqvppqvppqvhqvvaiatvvfiqplvttgslkthgvphgvwmvt
f**pkeitinavlqqwphvqlllhhfkltihiytylynfiinkk*yeni*nlxxk*ikni

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB771 (VHB771Q) /CSM/VH/VHB7-C/VHB771Q.Seq.d/                       1844   0.0
VHI101 (VHI101Q) /CSM/VH/VHI1-A/VHI101Q.Seq.d/                       1217   0.0
VHB683 (VHB683Q) /CSM/VH/VHB6-D/VHB683Q.Seq.d/                       1217   0.0
VFL517 (VFL517Q) /CSM/VF/VFL5-A/VFL517Q.Seq.d/                       1217   0.0
VFK358 (VFK358Q) /CSM/VF/VFK3-C/VFK358Q.Seq.d/                       1217   0.0
VFK835 (VFK835Q) /CSM/VF/VFK8-B/VFK835Q.Seq.d/                       1209   0.0
VFN428 (VFN428Q) /CSM/VF/VFN4-B/VFN428Q.Seq.d/                       1203   0.0
VFI407 (VFI407Q) /CSM/VF/VFI4-A/VFI407Q.Seq.d/                       1203   0.0
VFF871 (VFF871Q) /CSM/VF/VFF8-C/VFF871Q.Seq.d/                       1195   0.0
VFL437 (VFL437Q) /CSM/VF/VFL4-B/VFL437Q.Seq.d/                       1191   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U72746|U72746.1 Dictyostelium discoideum cysteine proteinase (cprG) mRNA, complete cds.  1142   0.0    1
U72745|U72745.1 Dictyostelium discoideum cysteine proteinase (cprF) mRNA, complete cds.   484   0.0    9
L36204|L36204.1 Dictyostelium discoideum cysteine proteinase (CP4) mRNA, complete cds.   131   e-126  8
L36205|L36205.1 Dictyostelium discoideum cysteine proteinase CP5 mRNA, complete cds.   147   e-125  8
AC117076|AC117076.3 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.   153   e-113  10
X03344|X03344.1 Dictyostelium discoideum mRNA for cysteine proteinase 2.    92   5e-35  4
M16039|M16039.1 Dictyostelium discoideum pst-cath gene encoding pst-cathepsin, complete cds.    72   6e-27  5
AJ489298|AJ489298.1 Aphis gossypii mRNA for putative cathepsin L (catL gene).    48   1e-13  4
X02407|X02407.1 D.discoideum mRNA for cysteine proteinase 1.       90   2e-13  1
ES225137|ES225137.1 MpSG_ag2_C10 Myzus persicae, tobacco lineage, aphid salivary gland library Myzus persicae cDNA clone MpSG_ag2_C10, mRNA sequence.    48   6e-13  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q94504) RecName: Full=Cysteine proteinase 7;          EC=3.4.22...   362   2e-98
(Q94503) RecName: Full=Cysteine proteinase 6;          EC=3.4.22...   319   1e-85
L36204_1(L36204|pid:none) Dictyostelium discoideum cysteine prot...   289   1e-76
(P54640) RecName: Full=Cysteine proteinase 5;          EC=3.4.22...   262   2e-68
L36205_1(L36205|pid:none) Dictyostelium discoideum cysteine prot...   256   1e-66
(P04989) RecName: Full=Cysteine proteinase 2;          EC=3.4.22...   209   2e-52
EF053509_1(EF053509|pid:none) Acanthamoeba castellanii cysteine ...   174   5e-42
AC117076_20(AC117076|pid:none) Dictyostelium discoideum chromoso...   166   1e-39
AY220615_1(AY220615|pid:none) Hydra vulgaris cathepsin L precurs...   147   8e-34
AJ784223_1(AJ784223|pid:none) Suberites domuncula mRNA for cathe...   147   8e-34

 psg: 0.77  gvh: 0.81  alm: 0.28  top: 0.33  tms: 0.07  mit: 0.36  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.77  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: Golgi
	 20.0 %: endoplasmic reticulum
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VHB771 is cyt

>VHB771F.Seq
ATTATCTTAATCATCCAATTATTTTTTAAATATATATATAAATAAAATGAAAGTTTTATC
TGCCTTATGCGTTTTATTAGTCAGTGTTGCCACTGCCAAACAACAATTATCCGAAGTTGA
ATACAGAAATGCTTTCACCAATTGGATGATTGCTCACCAAAGACATTATTCATCAGAAGA
GTTCAATGGTCGTTACAATATCTTCAAAGCCAATATGGATTACGTCAATGAATGGAATAC
CAAAGGTAGTGAAACCGTTTTAGGTTTAAATGTCTTTGCTGATATCAGCAATGAAGAATA
CAGAGCCACCTATTTAGGTACCCCATTCGATGCTTCATCCCTCGAAATGACCGAATCCGA
TAAAATCTTCGATGCTTCCGCTCAAGTCGATTGGAGAACTCAAGGTGCTGTCACCCCAAT
CAAAAATCAAGGTCAATGTGGTGGTTGTTGGTCATTCTCAACTACTGGTGCCACTGAAGG
TGCTCAATACCTTGCCAACGGTAAAAAGAATTTAGTCTCATTATCCGAACAAAATTTAAT
CGATTGTTCAGGTTCATACGGTAACAACGGTTGTGAAGGTGGTTTAATGACCTTAGCTTT
CGAATACATCATCANNNNNNNNNN

>VHB771Z.Seq
NNNNNNNNNNATACGTTTCAGGTATCTACAATGAGCCAGCTTGCTCTTCAACTCAATTAG
ATCACGGTGTCTTAGCTGTTGGTTTCGGTACTGGTAGTGGTTCATCTGGTTCCCATGGTG
GTTCCCAATCACAATCTGCTGGTTCAGATTCACAATCTGCTGGTTCAGAATCATCCCAAT
CTGAATCCGGTTCACAATCACAATCTGAATCCGGTTCACAATCACAATCACAATCTGGTT
CTCAATCATTCTCTGGTTCCTTATACTCTGGTTCATACTCTGGTTCCCAATCTGGTTCCC
AATCTGGTAACAGCGGTGCTGCTGTTAAACAAACTGGTGCTGGTTCAGGTTCAGGTTCAG
GTTCAGGTTCCGGTTCCGGTTCAGGTTCAGGTTCAGTCTCAGGTTCAGCTTCAGGTTCCG
CCTCAGGTTCCGCCTCAGGTTCATCAAGTGGTAGCAATAGCAACGGTGGTGTTTATCCAA
CCGCTGGTGACTACTGGATCGTTAAAAACTCATGGGGTACCTCATGGGGTATGGATGGTT
ACATTTTAATGACCAAAGGAAATAACAATCAATGCGGTATTGCAACAATGGCCTCACGTC
CAACTGCTGTTGCATCACTTTAAATTAACGATACATATATATACATATTTATATAATTTT
ATAATTAATAAAAAATAATACGAAAACATATAAAATTTATANNATAAATAAATAAAAAAT
ATT

>VHB771P.Seq
ATTATCTTAATCATCCAATTATTTTTTAAATATATATATAAATAAAATGAAAGTTTTATC
TGCCTTATGCGTTTTATTAGTCAGTGTTGCCACTGCCAAACAACAATTATCCGAAGTTGA
ATACAGAAATGCTTTCACCAATTGGATGATTGCTCACCAAAGACATTATTCATCAGAAGA
GTTCAATGGTCGTTACAATATCTTCAAAGCCAATATGGATTACGTCAATGAATGGAATAC
CAAAGGTAGTGAAACCGTTTTAGGTTTAAATGTCTTTGCTGATATCAGCAATGAAGAATA
CAGAGCCACCTATTTAGGTACCCCATTCGATGCTTCATCCCTCGAAATGACCGAATCCGA
TAAAATCTTCGATGCTTCCGCTCAAGTCGATTGGAGAACTCAAGGTGCTGTCACCCCAAT
CAAAAATCAAGGTCAATGTGGTGGTTGTTGGTCATTCTCAACTACTGGTGCCACTGAAGG
TGCTCAATACCTTGCCAACGGTAAAAAGAATTTAGTCTCATTATCCGAACAAAATTTAAT
CGATTGTTCAGGTTCATACGGTAACAACGGTTGTGAAGGTGGTTTAATGACCTTAGCTTT
CGAATACATCATCA----------ATACGTTTCAGGTATCTACAATGAGCCAGCTTGCTC
TTCAACTCAATTAGATCACGGTGTCTTAGCTGTTGGTTTCGGTACTGGTAGTGGTTCATC
TGGTTCCCATGGTGGTTCCCAATCACAATCTGCTGGTTCAGATTCACAATCTGCTGGTTC
AGAATCATCCCAATCTGAATCCGGTTCACAATCACAATCTGAATCCGGTTCACAATCACA
ATCACAATCTGGTTCTCAATCATTCTCTGGTTCCTTATACTCTGGTTCATACTCTGGTTC
CCAATCTGGTTCCCAATCTGGTAACAGCGGTGCTGCTGTTAAACAAACTGGTGCTGGTTC
AGGTTCAGGTTCAGGTTCAGGTTCCGGTTCCGGTTCAGGTTCAGGTTCAGTCTCAGGTTC
AGCTTCAGGTTCCGCCTCAGGTTCCGCCTCAGGTTCATCAAGTGGTAGCAATAGCAACGG
TGGTGTTTATCCAACCGCTGGTGACTACTGGATCGTTAAAAACTCATGGGGTACCTCATG
GGGTATGGATGGTTACATTTTAATGACCAAAGGAAATAACAATCAATGCGGTATTGCAAC
AATGGCCTCACGTCCAACTGCTGTTGCATCACTTTAAATTAACGATACATATATATACAT
ATTTATATAATTTTATAATTAATAAAAAATAATACGAAAACATATAAAATTTATANNATA
AATAAATAAAAAATATT