>VHB738 (VHB738Q) /CSM/VH/VHB7-B/VHB738Q.Seq.d/
CACACAATCATTACATGTTCGATTGTAGAATATTTCACAGATTTTTTTTATTTTAAAATA
AATTTCCGCAATTCCAACAAATAAAAAGAAATTATAGAGATAAAATTTAATATAATATAA
TATAAAAAAAAAAXXXXXXXXXXCGAACTTTTAGATATGTTGAAAGCTCATAAAAAAGGT
ATTCGTCGTGCCGCTGTAAATACATTTGGTTACATTGCAAAAGCTATTGGTCCTCAAGAG
GTATTGGCAACCCTATTAAACAATTTGAAAGTTCAAGATCGTCAAAACAGAGTTTGTACA
ACCGTTGCCATTGCCATTGTTGCGGAAACTTGTGCACCTTATACCGTTTTACCAGGTCTA
ATCAATGAATATCGTATTCCAGAGTTGAATGTTCAAAATGGTGTTTTGAAATCTTTATCT
TTCCTCTTTGAATATATTGGCGAAATGGGTAAAGACTATATTTACGCCGTAACTACCCTA
CTCGAAGATGCACTCATGGATAGAGATGCTGTACATCGTCAAACCGCTTGCTCTGCAATT
AAACATATTTCATTGGGTGTAATGGGTTTAGGTTGTGAAGATTCACTCACTCATTTACTC
AATTACGTTTGGCCAAATGTTTTCGAAACTTCTCCACATGTTATCAACGCTTTCTTAGAG
GC

tqslhvrl*nisqiffilk*isaiptnkkkl*r*nli*YNIKKK---

---ELLDMLKAHKKGIRRAAVNTFGYIAKAIGPQEVLATLLNNLKVQDRQNRVCTTVAIA
IVAETCAPYTVLPGLINEYRIPELNVQNGVLKSLSFLFEYIGEMGKDYIYAVTTLLEDAL
MDRDAVHRQTACSAIKHISLGVMGLGCEDSLTHLLNYVWPNVFETSPHVINAFLE

Frame A:
htiitcsiveyftdffyfkinfrnsnk*keiieikfnii*ykkk---

---rtfryvess*krysscrckyiwlhcksywssrgignpikqfessrsskqslynrchc
hccgnlctlyrftrsnq*isysrvecskwcfeififpl*iywrng*rlylrrnyptrrct
hg*rcctssnrllcn*tyfigcngfrl*rfthsftqlrlakcfrnfstcyqrflrg

Frame B:
tqslhvrl*nisqiffilk*isaiptnkkkl*r*nli*YNIKKK---

---ELLDMLKAHKKGIRRAAVNTFGYIAKAIGPQEVLATLLNNLKVQDRQNRVCTTVAIA
IVAETCAPYTVLPGLINEYRIPELNVQNGVLKSLSFLFEYIGEMGKDYIYAVTTLLEDAL
MDRDAVHRQTACSAIKHISLGVMGLGCEDSLTHLLNYVWPNVFETSPHVINAFLE

Frame C:
hnhymfdcrifhrfflf*nkfpqfqqikrnyrdki*ynii*kk---

---nf*ic*klikkvfvvpl*ihlvtlqkllvlkrywqpy*ti*kfkivktefvqplplp
llrklvhlipfyqv*smnivfqs*mfkmvf*nlylsslnilakwvktiftp*lpyskmhs
wiemlyivkplalqlnifhwv*wv*vvkihsliysitfgqmfskllhmlstls*r

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB738 (VHB738Q) /CSM/VH/VHB7-B/VHB738Q.Seq.d/                       1029   0.0
AHM560 (AHM560Q) /CSM/AH/AHM5-C/AHM560Q.Seq.d/                       1029   0.0
AHD733 (AHD733Q) /CSM/AH/AHD7-B/AHD733Q.Seq.d/                       1029   0.0
AHD177 (AHD177Q) /CSM/AH/AHD1-D/AHD177Q.Seq.d/                       1029   0.0
CHF570 (CHF570Q) /CSM/CH/CHF5-C/CHF570Q.Seq.d/                       1025   0.0
CHM817 (CHM817Q) /CSM/CH/CHM8-A/CHM817Q.Seq.d/                       1021   0.0
SHA183 (SHA183Q) /CSM/SH/SHA1-D/SHA183Q.Seq.d/                       1015   0.0
CHO828 (CHO828Q) /CSM/CH/CHO8-B/CHO828Q.Seq.d/                       1015   0.0
CHM843 (CHM843Q) /CSM/CH/CHM8-B/CHM843Q.Seq.d/                       1015   0.0
AHA193 (AHA193Q) /CSM/AH/AHA1-D/AHA193Q.Seq.d/                       1015   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117082|AC117082.2 Dictyostelium discoideum chromosome 2 map complement(6807709-6776860) strain AX4, complete sequence.  1029   0.0    2
AU312337|AU312337.1 Elaphe quadrivirgata cDNA, clone:Eq_aB_021_F03.   109   9e-44  3
EC286335|EC286335.1 BRAINF089834J4 POSSUM_01-POSSUM-C-BRAIN-2KB Trichosurus vulpecula cDNA clone 1061024733099, mRNA sequence.    92   8e-40  4
EC364387|EC364387.1 IMMUNEF093542N20 POSSUM_01-C-POSSUM-IMMUNE-2KB Trichosurus vulpecula cDNA clone 1061024549274, mRNA sequence.    92   1e-39  4
EC289560|EC289560.1 BRAINF089623B7 POSSUM_01-POSSUM-C-BRAIN-2KB Trichosurus vulpecula cDNA clone 1061024822769, mRNA sequence.    92   2e-36  4
CX303109|CX303109.1 C08027F07SK PhyRootSw1 Citrus sinensis cDNA clone C08027F07, mRNA sequence.    78   4e-33  3
DV664609|DV664609.1 CGN-1811 Pericarp Coffea canephora cDNA clone cccp22c1 5', mRNA sequence.   100   1e-32  5
DY611266|DY611266.1 IMMUNEF045822K24 POSSUM_01-C-POSSUM-IMMUNE-2KB Trichosurus vulpecula cDNA clone 1061021063254, mRNA sequence.    92   3e-31  3
DY031641|DY031641.1 zfi02-5ms1-e05 Zfi02 Zamia fischeri cDNA clone zfi02-5ms1-e05 5', mRNA sequence.   105   1e-30  3
BJ615493|BJ615493.1 Xenopus laevis cDNA clone:XL172e10, 5' end.    68   3e-26  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AP008208_305(AP008208|pid:none) Oryza sativa (japonica cultivar-...   313   4e-84
AK221391_1(AK221391|pid:none) Arabidopsis thaliana mRNA for nucl...   311   1e-83
AE014134_132(AE014134|pid:none) Drosophila melanogaster chromoso...   306   5e-82
(O57683) RecName: Full=Splicing factor 3B subunit 1; AltName: Fu...   300   3e-80
(O75533) RecName: Full=Splicing factor 3B subunit 1; AltName: Fu...   300   3e-80
CP001324_246(CP001324|pid:none) Micromonas sp. RCC299 chromosome...   299   6e-80
BC172813_1(BC172813|pid:none) Synthetic construct Homo sapiens c...   293   3e-78
CP000583_21(CP000583|pid:none) Ostreococcus lucimarinus CCE9901 ...   292   5e-78
AM270010_46(AM270010|pid:none) Aspergillus niger contig An02c015...   289   6e-77
AP007159_186(AP007159|pid:none) Aspergillus oryzae RIB40 genomic...   289   6e-77

 psg: 0.54  gvh: 0.36  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.13  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHB738 is nuc

>VHB738F.Seq
CACACAATCATTACATGTTCGATTGTAGAATATTTCACAGATTTTTTTTATTTTAAAATA
AATTTCCGCAATTCCAACAAATAAAAAGAAATTATAGAGATAAAATTTAATATAATATAA
TATAAAAAAAAAANNNNNNNNNN

>VHB738Z.Seq
NNNNNNNNNNCGAACTTTTAGATATGTTGAAAGCTCATAAAAAAGGTATTCGTCGTGCCG
CTGTAAATACATTTGGTTACATTGCAAAAGCTATTGGTCCTCAAGAGGTATTGGCAACCC
TATTAAACAATTTGAAAGTTCAAGATCGTCAAAACAGAGTTTGTACAACCGTTGCCATTG
CCATTGTTGCGGAAACTTGTGCACCTTATACCGTTTTACCAGGTCTAATCAATGAATATC
GTATTCCAGAGTTGAATGTTCAAAATGGTGTTTTGAAATCTTTATCTTTCCTCTTTGAAT
ATATTGGCGAAATGGGTAAAGACTATATTTACGCCGTAACTACCCTACTCGAAGATGCAC
TCATGGATAGAGATGCTGTACATCGTCAAACCGCTTGCTCTGCAATTAAACATATTTCAT
TGGGTGTAATGGGTTTAGGTTGTGAAGATTCACTCACTCATTTACTCAATTACGTTTGGC
CAAATGTTTTCGAAACTTCTCCACATGTTATCAACGCTTTCTTAGAGGC

>VHB738P.Seq
CACACAATCATTACATGTTCGATTGTAGAATATTTCACAGATTTTTTTTATTTTAAAATA
AATTTCCGCAATTCCAACAAATAAAAAGAAATTATAGAGATAAAATTTAATATAATATAA
TATAAAAAAAAAA----------CGAACTTTTAGATATGTTGAAAGCTCATAAAAAAGGT
ATTCGTCGTGCCGCTGTAAATACATTTGGTTACATTGCAAAAGCTATTGGTCCTCAAGAG
GTATTGGCAACCCTATTAAACAATTTGAAAGTTCAAGATCGTCAAAACAGAGTTTGTACA
ACCGTTGCCATTGCCATTGTTGCGGAAACTTGTGCACCTTATACCGTTTTACCAGGTCTA
ATCAATGAATATCGTATTCCAGAGTTGAATGTTCAAAATGGTGTTTTGAAATCTTTATCT
TTCCTCTTTGAATATATTGGCGAAATGGGTAAAGACTATATTTACGCCGTAACTACCCTA
CTCGAAGATGCACTCATGGATAGAGATGCTGTACATCGTCAAACCGCTTGCTCTGCAATT
AAACATATTTCATTGGGTGTAATGGGTTTAGGTTGTGAAGATTCACTCACTCATTTACTC
AATTACGTTTGGCCAAATGTTTTCGAAACTTCTCCACATGTTATCAACGCTTTCTTAGAG
GC