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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB734
Library VH
(Link to library)
Clone ID VHB734
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U13636-1
Original site URL
Representative seq. ID VHB734P
(Link to Original site)
Representative DNA sequence 
>VHB734 (VHB734Q) /CSM/VH/VHB7-B/VHB734Q.Seq.d/
ATTTAAAATTTCTTTAATCTTTGTAAATATATATAAAAATGGGTTTAGAACAAGATAAGC
AACCATTAATGGGACAACAACCAGTTTACCAAGGTTACCAACAACAACCACAATATGCTT
ACCCACAACAACCAGTTTATGCTCAACAACCAGTTTATGCTCAACAACCAGTTTATGCAA
CTACTGTAACTACATCTGACCATCATCATCATCATCATCATGATGGAGGATATACTGGAG
CTTTGATTTTCTTTATTTGTGGCTTCTTCATCTCATTACTTTGGTTAGGTGGTTTCTATT
ATATTAGATCTCACAACAGAACTGCTAGATCTTTAGGTATCGCCTCAGTCGTTTTATTCT
TTGTCATGTTAGTTATTGTTATCATCGTTTTAGCTTCAGGTGCAAATTAAATATAAAATC
AAATTCTTATTGAAAAAAAAAAXXXXXXXXXXATTTAAAATTTCTTTAATCTTTGTAAAT
ATATATAAAAATGGGTTTAGAACAAGATAAGCAACCATTAATGGGACAACAACCAGTTTA
CCAAGGTTACCAACAACAACCACAATATGCTTACCCACAACAACCAGTTTATGCTCAACA
ACCAGTTTATGCTCAACAACCAGTTTATGCAACTACTGTAACTACATCTGACCATCATCA
TCATCATCATCATGATGGAGGATATACTGGAGCTTTGATTTTCTTTATTTGTGGCTTCTT
CATCTCATTACTTTGGTTAGGTGGTTTCTATTATATTAGATCTCACAACAGAACTGCTAG
ATCTTTAGGTATCGCCTCAGTCGTTTTATTCTATTGTCATGTTAGTTATTGTTATCATCG
TTT
sequence update 2002.10.25
Translated Amino Acid sequence 
i*nffnlckyi*kwv*nkisnh*wdnnqftkvtnnnhnmlthnnqfmlnnqfmlnnqfmq
ll*lhltiiiiiimmedilel*fslfvassshyfg*vvsiildlttelldl*vspqsfys
lsc*lllssf*LQVQIKYKIKFLLKKK---

---FKISLIFVNIYKNGFRTR*atingtttslprlptttticlptttslcsttslcstts
lcnycnyi*pssssss*wriywsfdflylwllhlitlvrwflly*isqqnc*ifryrlsr
fillsc*lllssf
Translated Amino Acid sequence (All Frames) 
Frame A:
i*nffnlckyi*kwv*nkisnh*wdnnqftkvtnnnhnmlthnnqfmlnnqfmlnnqfmq
ll*lhltiiiiiimmedilel*fslfvassshyfg*vvsiildlttelldl*vspqsfys
lsc*lllssf*LQVQIKYKIKFLLKKK---

---i*nffnlckyi*kwv*nkisnh*wdnnqftkvtnnnhnmlthnnqfmlnnqfmlnnq
fmqll*lhltiiiiiimmedilel*fslfvassshyfg*vvsiildlttelldl*vspqs
fysivmlviviiv

Frame B:
fkislifvniykngfrtr*atingtttslprlptttticlptttslcsttslcsttslcn
ycnyi*pssssss*wriywsfdflylwllhlitlvrwflly*isqqnc*ifryrlsrfil
chvsycyhrfsfrcklniksnsy*kkk---

---FKISLIFVNIYKNGFRTR*atingtttslprlptttticlptttslcsttslcstts
lcnycnyi*pssssss*wriywsfdflylwllhlitlvrwflly*isqqnc*ifryrlsr
fillsc*lllssf

Frame C:
lkfl*sl*iyikmgleqdkqplmgqqpvyqgyqqqpqyaypqqpvyaqqpvyaqqpvyat
tvttsdhhhhhhhdggytgalifficgffisllwlggfyyirshnrtarslgiasvvlff
vmlviviivlasgan*i*nqiliekk---

---lkfl*sl*iyikmgleqdkqplmgqqpvyqgyqqqpqyaypqqpvyaqqpvyaqqpv
yattvttsdhhhhhhhdggytgalifficgffisllwlggfyyirshnrtarslgiasvv
lfychvsycyhr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF531 (VSF531Q) /CSM/VS/VSF5-B/VSF531Q.Seq.d/                        418   e-116
VHB734 (VHB734Q) /CSM/VH/VHB7-B/VHB734Q.Seq.d/                        418   e-116
SSG712 (SSG712Q) /CSM/SS/SSG7-A/SSG712Q.Seq.d/                        418   e-116
VSC686 (VSC686Q) /CSM/VS/VSC6-D/VSC686Q.Seq.d/                        408   e-113
VSH734 (VSH734Q) /CSM/VS/VSH7-B/VSH734Q.Seq.d/                        400   e-110
VHF529 (VHF529Q) /CSM/VH/VHF5-B/VHF529Q.Seq.d/                        396   e-109
VSA701 (VSA701Q) /CSM/VS/VSA7-A/VSA701Q.Seq.d/                        313   2e-84
VSD673 (VSD673Q) /CSM/VS/VSD6-D/VSD673Q.Seq.d/                        180   2e-44
VSI216 (VSI216Q) /CSM/VS/VSI2-A/VSI216Q.Seq.d/                         42   0.014
VSD175 (VSD175Q) /CSM/VS/VSD1-D/VSD175Q.Seq.d/                         42   0.014
own update 2002.12.23
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AM652951|AM652951.1 Entamoeba moshkovskii FIC GSS, clone mosh124f04.p1k.    56   1e-04  2
AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.    36   2e-04  10
BE194477|BE194477.1 HVSMEh0085L07f Hordeum vulgare 5-45 DAP spike EST library HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare cDNA clone HVSMEh0085L07f, mRNA sequence.    30   0.001  5
AJ486496|AJ486496.1 Hordeum vulgare EST clone S0001100178C07F1.    30   0.002  4
AJ479943|AJ479943.1 Hordeum vulgare EST clone S0001100129H12F1.    30   0.002  4
AJ479935|AJ479935.1 Hordeum vulgare EST clone S0001100093C08F1.    30   0.002  4
AJ479934|AJ479934.1 Hordeum vulgare EST clone S0001100086C10F1.    30   0.002  4
AJ479930|AJ479930.1 Hordeum vulgare EST clone S0001100070B05F1.    30   0.002  4
CK809027|CK809027.1 Rasgsc2119 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    32   0.002  4
CK809282|CK809282.1 Rasgsc2702 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    32   0.002  4
dna update 2007. 4.15
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 760,476,831
protein update 2009. 6.27
PSORT 

 psg: 0.52  gvh: 0.22  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.41  mip: 0.00
 nuc: 0.12  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 20.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for VHB734 is nuc
5' end seq. ID VHB734F
5' end seq. 
>VHB734F.Seq
ATTTAAAATTTCTTTAATCTTTGTAAATATATATAAAAATGGGTTTAGAACAAGATAAGC
AACCATTAATGGGACAACAACCAGTTTACCAAGGTTACCAACAACAACCACAATATGCTT
ACCCACAACAACCAGTTTATGCTCAACAACCAGTTTATGCTCAACAACCAGTTTATGCAA
CTACTGTAACTACATCTGACCATCATCATCATCATCATCATGATGGAGGATATACTGGAG
CTTTGATTTTCTTTATTTGTGGCTTCTTCATCTCATTACTTTGGTTAGGTGGTTTCTATT
ATATTAGATCTCACAACAGAACTGCTAGATCTTTAGGTATCGCCTCAGTCGTTTTATTCT
TTGTCATGTTAGTTATTGTTATCATCGTTTTAGCTTCAGGTGCAAATTAAATATAAAATC
AAATTCTTATTGAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 452
3' end seq. ID VHB734Z
3' end seq. 
>VHB734Z.Seq
NNNNNNNNNNATTTAAAATTTCTTTAATCTTTGTAAATATATATAAAAATGGGTTTAGAA
CAAGATAAGCAACCATTAATGGGACAACAACCAGTTTACCAAGGTTACCAACAACAACCA
CAATATGCTTACCCACAACAACCAGTTTATGCTCAACAACCAGTTTATGCTCAACAACCA
GTTTATGCAACTACTGTAACTACATCTGACCATCATCATCATCATCATCATGATGGAGGA
TATACTGGAGCTTTGATTTTCTTTATTTGTGGCTTCTTCATCTCATTACTTTGGTTAGGT
GGTTTCTATTATATTAGATCTCACAACAGAACTGCTAGATCTTTAGGTATCGCCTCAGTC
GTTTTATTCTATTGTCATGTTAGTTATTGTTATCATCGTTT
Length of 3' end seq. 401
Connected seq. ID VHB734P
Connected seq. 
>VHB734P.Seq
ATTTAAAATTTCTTTAATCTTTGTAAATATATATAAAAATGGGTTTAGAACAAGATAAGC
AACCATTAATGGGACAACAACCAGTTTACCAAGGTTACCAACAACAACCACAATATGCTT
ACCCACAACAACCAGTTTATGCTCAACAACCAGTTTATGCTCAACAACCAGTTTATGCAA
CTACTGTAACTACATCTGACCATCATCATCATCATCATCATGATGGAGGATATACTGGAG
CTTTGATTTTCTTTATTTGTGGCTTCTTCATCTCATTACTTTGGTTAGGTGGTTTCTATT
ATATTAGATCTCACAACAGAACTGCTAGATCTTTAGGTATCGCCTCAGTCGTTTTATTCT
TTGTCATGTTAGTTATTGTTATCATCGTTTTAGCTTCAGGTGCAAATTAAATATAAAATC
AAATTCTTATTGAAAAAAAAAA----------ATTTAAAATTTCTTTAATCTTTGTAAAT
ATATATAAAAATGGGTTTAGAACAAGATAAGCAACCATTAATGGGACAACAACCAGTTTA
CCAAGGTTACCAACAACAACCACAATATGCTTACCCACAACAACCAGTTTATGCTCAACA
ACCAGTTTATGCTCAACAACCAGTTTATGCAACTACTGTAACTACATCTGACCATCATCA
TCATCATCATCATGATGGAGGATATACTGGAGCTTTGATTTTCTTTATTTGTGGCTTCTT
CATCTCATTACTTTGGTTAGGTGGTTTCTATTATATTAGATCTCACAACAGAACTGCTAG
ATCTTTAGGTATCGCCTCAGTCGTTTTATTCTATTGTCATGTTAGTTATTGTTATCATCG
TTT
Length of connected seq. 833
Full length Seq ID -
Full length Seq. -
Length of full length seq. -