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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB729
Library VH
(Link to library)
Clone ID VHB729
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHB729P
(Link to Original site)
Representative DNA sequence 
>VHB729 (VHB729Q) /CSM/VH/VHB7-B/VHB729Q.Seq.d/
ACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTCG
TGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATCA
CGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAGT
TTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCAT
TAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTGG
TTGCACTTCGCACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCAG
TTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCGT
CGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCAA
ACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTGA
AGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACAC
TGAAGATAAGGAATTCGGTGATGTCACTGTATCCCCAGAXXXXXXXXXXTCTCGCTGATA
ACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACG
GTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATT
CTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGA
GAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTG
GTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCC
CAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTG
GTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCG
GTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCG
CTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGG
CTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAA
AGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAA
CAATTTAAGTCGCACTGAGTTTTCAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVTVSP---

---LADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGV
VCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITA
PENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQ
CVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*valsfq
Translated Amino Acid sequence (All Frames) 
Frame A:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlhfarifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r*gir*chcipr---

---sr**prmg*k*chehlvfrsrr*rcqsprqchqrcsife*n*rffrwcfpmghqrrc
cl**kherypfqsl*chpph*chpqrwwsnhpncssctlcc*inclpnslrtnllsrnyc
prkrywwyllctqss*rycyw*rtsyrfpiilcqssftsirilrfhr*ftfphcrssfpt
mclrslgfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnlsrtefs

Frame B:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVTVSP---

---LADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGV
VCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITA
PENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQ
CVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*valsfq

Frame C:
srvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpikf
lvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvalrtnf*silsipqvtlisv
qk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tlk
klislsvellnpsmsllvtlkirnsvmslypq---

---slittngikmmp*tfglsvqkvtvpisssmspkvfni*mklkilslvlsngppkkvl
fvmkt*evsvsifmmspstlmpstevvvkssqllvvysmlln*lppqls*nqst**kllp
qktllvvftlysivvevlllvknvvsvphyslskliyqy*npsvspliyvptlpvklshn
vssiiglqlvlstkirkplksl*pperekv*pqksqiltnstknskqfksh*vfk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB729 (VHB729Q) /CSM/VH/VHB7-B/VHB729Q.Seq.d/                       2573   0.0
VHO711 (VHO711Q) /CSM/VH/VHO7-A/VHO711Q.Seq.d/                       1384   0.0
VHK672 (VHK672Q) /CSM/VH/VHK6-C/VHK672Q.Seq.d/                       1384   0.0
VHK338 (VHK338Q) /CSM/VH/VHK3-B/VHK338Q.Seq.d/                       1384   0.0
VHJ541 (VHJ541Q) /CSM/VH/VHJ5-B/VHJ541Q.Seq.d/                       1384   0.0
VHI270 (VHI270Q) /CSM/VH/VHI2-C/VHI270Q.Seq.d/                       1384   0.0
VHH502 (VHH502Q) /CSM/VH/VHH5-A/VHH502Q.Seq.d/                       1384   0.0
VHD772 (VHD772Q) /CSM/VH/VHD7-C/VHD772Q.Seq.d/                       1384   0.0
VHD422 (VHD422Q) /CSM/VH/VHD4-A/VHD422Q.Seq.d/                       1384   0.0
VHC630 (VHC630Q) /CSM/VH/VHC6-B/VHC630Q.Seq.d/                       1384   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.  1257   0.0    2
AF107291|AF107291.1 Candida parapsilosis elongation factor 2 (EFT2) gene, partial cds.    72   6e-71  13
AF107286|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds.    78   2e-70  9
Y09664|Y09664.1 C.albicans EFT2 gene.                              78   4e-69  9
AF107289|AF107289.1 Candida tropicalis elongation factor 2 (EFT2) gene, partial cds.    70   6e-66  10
AF107290|AF107290.1 Candida lusitaniae elongation factor 2 (EFT2) gene, partial cds.    64   5e-49  8
AF534908|AF534908.2 Tetrahymena thermophila elongation factor 2 gene, complete cds.    84   4e-47  6
AY144999|AY144999.1 Saccharomyces kluyveri clone Contig1706 EFT2 gene, complete cds.    78   4e-45  7
AR550596|AR550596.1 Sequence 5727 from patent US 6747137.          78   2e-44  6
EH015431|EH015431.1 USDA-FP_188032 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt081_G08 5', mRNA sequence.    78   6e-43  4
dna update 2007. 4.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   291   3e-77
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   289   2e-76
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   289   2e-76
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   289   2e-76
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   288   3e-76
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   288   4e-76
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   287   5e-76
AK298498_1(AK298498|pid:none) Homo sapiens cDNA FLJ56548 complet...   285   3e-75
M19997_1(M19997|pid:none) Human elongation factor 2 (EF-2) mRNA,...   285   3e-75
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   285   3e-75
protein update 2009. 6.27
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHB729 is cyt
5' end seq. ID VHB729F
5' end seq. 
>VHB729F.Seq
ACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTCG
TGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATCA
CGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAGT
TTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCAT
TAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTGG
TTGCACTTCGCACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCAG
TTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCGT
CGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCAA
ACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTGA
AGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACAC
TGAAGATAAGGAATTCGGTGATGTCACTGTATCCCCAGANNNNNNNNNN
Length of 5' end seq. 649
3' end seq. ID VHB729Z
3' end seq. 
>VHB729Z.Seq
NNNNNNNNNNTCTCGCTGATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGT
CTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAAT
ATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTG
TTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACA
CTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATG
CTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTG
CCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTA
TTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTAT
TAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCAC
AATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTG
AAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACA
AATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTGAGTTTTCAAA
Length of 3' end seq. 708
Connected seq. ID VHB729P
Connected seq. 
>VHB729P.Seq
ACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTCG
TGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATCA
CGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAGT
TTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCAT
TAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTGG
TTGCACTTCGCACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCAG
TTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCGT
CGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCAA
ACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTGA
AGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACAC
TGAAGATAAGGAATTCGGTGATGTCACTGTATCCCCAGA----------TCTCGCTGATA
ACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACG
GTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATT
CTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGA
GAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTG
GTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCC
CAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTG
GTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCG
GTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCG
CTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGG
CTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAA
AGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAA
CAATTTAAGTCGCACTGAGTTTTCAAA
Length of connected seq. 1337
Full length Seq ID -
Full length Seq. -
Length of full length seq. -