>VHB725 (VHB725Q) /CSM/VH/VHB7-B/VHB725Q.Seq.d/
XXXXXXXXXXGTGCAGGTGCTGTCGAAGGTTCATGTCGTTGGTGGTCAAGAGATCATCGT
GCTCATCATCGTTATCTCGATACTGATAAAGATCCATATTCAGCTTCATTGGGTTTCTGG
TATTCCCATTTCATTTGGTTAATGTTCAAACAAGATCCAAAGAAAATCGGTAGAGCAAAC
ATTGACGACCTTAACGCCGATCCATGGGTTATGTGGCAACATAAACATTACATTAAAATC
GCCACTTTTATGGGTCTCGTTTTCCCAACATTGGTAGCTGGTTTAGGTTGGGGTGATTGG
GCTGGTGGTTATTTCTACTCTGGTGTACTTCGTCTCGTTACAGTTCAACATTCCACATTC
TTGGTCAATTCATTGGCTCATTATTTAGGTGACTCTCCATACGATGATGAACATACTCCA
AAGGATTCAGTTGTGACTGCTATATTAACATTTGGTGAAGGTTATCATAACTTCCATCAT
GAATTCCCAAATGACTATAGAAACGCTTATAAATTCTATCAATATGATCCAACTAAATGG
TTGATCTCTGCAATGTA

---AGAVEGSCRWWSRDHRAHHRYLDTDKDPYSASLGFWYSHFIWLMFKQDPKKIGRANI
DDLNADPWVMWQHKHYIKIATFMGLVFPTLVAGLGWGDWAGGYFYSGVLRLVTVQHSTFL
VNSLAHYLGDSPYDDEHTPKDSVVTAILTFGEGYHNFHHEFPNDYRNAYKFYQYDPTKWL
ISAM

Frame A:
---crccrrfmslvvkrsscssslsry**rsifsfigflvfpfhlvnvqtrskenr*skh
*rp*rrsmgyvat*tlh*nrhfygsrfpnigswfrlg*lgwwlfllwctssrysstfhil
gqfigslfr*lsir**tyskgfscdcyiniw*rls*lps*ipk*l*krl*ilsi*sn*mv
dlcnv


Frame B:
---AGAVEGSCRWWSRDHRAHHRYLDTDKDPYSASLGFWYSHFIWLMFKQDPKKIGRANI
DDLNADPWVMWQHKHYIKIATFMGLVFPTLVAGLGWGDWAGGYFYSGVLRLVTVQHSTFL
VNSLAHYLGDSPYDDEHTPKDSVVTAILTFGEGYHNFHHEFPNDYRNAYKFYQYDPTKWL
ISAM


Frame C:
---qvlskvhvvggqeiivliivisilikihiqlhwvsgipisfg*csnkiqrksveqtl
ttltpihglcgninitlkspllwvsfsqhw*lv*vgviglvvistlvyfvslqfniphsw
sihwlii*vtlhtmmnilqriql*lly*hlvkviitsimnsqmtietlinsinmiqlng*
slqc

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB725 (VHB725Q) /CSM/VH/VHB7-B/VHB725Q.Seq.d/                       1084   0.0
VHP282 (VHP282Q) /CSM/VH/VHP2-D/VHP282Q.Seq.d/                       1076   0.0
VFM428 (VFM428Q) /CSM/VF/VFM4-B/VFM428Q.Seq.d/                       1068   0.0
VHI633 (VHI633Q) /CSM/VH/VHI6-B/VHI633Q.Seq.d/                       1061   0.0
VHP359 (VHP359Q) /CSM/VH/VHP3-C/VHP359Q.Seq.d/                       1053   0.0
VHL555 (VHL555Q) /CSM/VH/VHL5-C/VHL555Q.Seq.d/                       1051   0.0
VHJ240 (VHJ240Q) /CSM/VH/VHJ2-B/VHJ240Q.Seq.d/                       1047   0.0
VFA222 (VFA222Q) /CSM/VF/VFA2-A/VFA222Q.Seq.d/                       1047   0.0
VHE611 (VHE611Q) /CSM/VH/VHE6-A/VHE611Q.Seq.d/                       1045   0.0
VHK887 (VHK887Q) /CSM/VH/VHK8-D/VHK887Q.Seq.d/                        993   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.  1074   0.0    1
DY886427|DY886427.1 CeleSEQ2079 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ2079, mRNA sequence.    68   2e-25  4
DY892961|DY892961.1 CeleSEQ12104 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ12104, mRNA sequence.    68   2e-25  4
DY888809|DY888809.1 CeleSEQ6138 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ6138, mRNA sequence.    68   3e-24  4
DY895351|DY895351.1 CeleSEQ15152 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ15152, mRNA sequence.    52   8e-19  4
DY885896|DY885896.1 CeleSEQ991 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ991, mRNA sequence.    52   2e-18  4
DY885119|DY885119.1 CeleSEQ310 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ310, mRNA sequence.    68   4e-17  3
DY885952|DY885952.1 CeleSEQ1408 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ1408, mRNA sequence.    68   6e-17  3
DY885558|DY885558.1 CeleSEQ846 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ846, mRNA sequence.    68   8e-17  3
DY890339|DY890339.1 CeleSEQ9316 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ9316, mRNA sequence.    68   2e-16  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116984_117(AC116984|pid:none) Dictyostelium discoideum chromos...   400   e-110
AY576801_1(AY576801|pid:none) Hortaea werneckii putative delta-9...   287   1e-76
EU857727_1(EU857727|pid:none) Hortaea werneckii delta-9 desatura...   287   1e-76
Y18554_1(Y18554|pid:none) Mortierella alpina ole2 gene for stear...   286   3e-76
DQ901954_1(DQ901954|pid:none) Aureobasidium pullulans putative d...   284   1e-75
S52746(S52746;S52745) stearoyl-CoA 9-desaturase (EC 1.14.19.1) [...   282   4e-75
(Q12618) RecName: Full=Acyl-CoA desaturase;          EC=1.14.19....   280   1e-74
AM920435_140(AM920435|pid:none) Penicillium chrysogenum Wisconsi...   279   3e-74
AB195980_1(AB195980|pid:none) Mortierella alpina gene for omega9...   279   3e-74
AP007162_283(AP007162|pid:none) Aspergillus oryzae RIB40 genomic...   278   5e-74

 psg: 0.43  gvh: 0.38  alm: 0.32  top: 0.40  tms: 0.07  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 20.0 %: mitochondrial
	 20.0 %: nuclear
	 20.0 %: endoplasmic reticulum
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VHB725 is cyt

>VHB725Z.Seq
NNNNNNNNNNGTGCAGGTGCTGTCGAAGGTTCATGTCGTTGGTGGTCAAGAGATCATCGT
GCTCATCATCGTTATCTCGATACTGATAAAGATCCATATTCAGCTTCATTGGGTTTCTGG
TATTCCCATTTCATTTGGTTAATGTTCAAACAAGATCCAAAGAAAATCGGTAGAGCAAAC
ATTGACGACCTTAACGCCGATCCATGGGTTATGTGGCAACATAAACATTACATTAAAATC
GCCACTTTTATGGGTCTCGTTTTCCCAACATTGGTAGCTGGTTTAGGTTGGGGTGATTGG
GCTGGTGGTTATTTCTACTCTGGTGTACTTCGTCTCGTTACAGTTCAACATTCCACATTC
TTGGTCAATTCATTGGCTCATTATTTAGGTGACTCTCCATACGATGATGAACATACTCCA
AAGGATTCAGTTGTGACTGCTATATTAACATTTGGTGAAGGTTATCATAACTTCCATCAT
GAATTCCCAAATGACTATAGAAACGCTTATAAATTCTATCAATATGATCCAACTAAATGG
TTGATCTCTGCAATGTA