>VHB715 (VHB715Q) /CSM/VH/VHB7-A/VHB715Q.Seq.d/
GGAACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAAT
TCGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGA
TCACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAA
AGTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCAC
CATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGC
TGGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTT
CAGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTG
CGTCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTAT
CAAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACAC
TGAAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAA
CACTGAAGXXXXXXXXXXATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGT
CTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAAT
ATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTG
TTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACA
CTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATG
CTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTG
CCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTA
TTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTAT
TAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCAC
AATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTG
AAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACA
AATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAANATAGTAATC
AAATCTAAAAAAAATTTTAAAAAAA

ELSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADK
VSGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDF
SSEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNT
EEAYLSFRRAIESVNVIVGNTE---

---NHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCD
ENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITAPEN
AIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVF
DHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*val*vfkxsnqi*k
kf*k

Frame A:
gtleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*
sfw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*f
qfrsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh
*rslslfp*sy*irqchcw*h*---

---ittngikmmp*tfglsvqkvtvpisssmspkvfni*mklkilslvlsngppkkvlfv
mkt*evsvsifmmspstlmpstevvvkssqllvvysmlln*lppqls*nqst**kllpqk
tllvvftlysivvevlllvknvvsvphyslskliyqy*npsvspliyvptlpvklshnvs
siiglqlvlstkirkplksl*pperekv*pqksqiltnstknskqfkshfefsxiviksk
knfkk

Frame B:
ELSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADK
VSGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDF
SSEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNT
EEAYLSFRRAIESVNVIVGNTE---

---*prmg*k*chehlvfrsrr*rcqsprqchqrcsife*n*rffrwcfpmghqrrccl*
*kherypfqsl*chpph*chpqrwwsnhpncssctlcc*inclpnslrtnllsrnycprk
rywwyllctqss*rycyw*rtsyrfpiilcqssftsirilrfhr*ftfphcrssfptmcl
rslgfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnlsrtlsfqx**snlk
kilkk

Frame C:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlk---

---NHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCD
ENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITAPEN
AIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVF
DHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*val*vfkxsnqi*k
kf*k

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB715 (VHB715Q) /CSM/VH/VHB7-A/VHB715Q.Seq.d/                       2522   0.0
VHJ871 (VHJ871Q) /CSM/VH/VHJ8-C/VHJ871Q.Seq.d/                       1398   0.0
VHH842 (VHH842Q) /CSM/VH/VHH8-B/VHH842Q.Seq.d/                       1398   0.0
VHH819 (VHH819Q) /CSM/VH/VHH8-A/VHH819Q.Seq.d/                       1398   0.0
VHD427 (VHD427Q) /CSM/VH/VHD4-B/VHD427Q.Seq.d/                       1398   0.0
VHD139 (VHD139Q) /CSM/VH/VHD1-B/VHD139Q.Seq.d/                       1398   0.0
VHD106 (VHD106Q) /CSM/VH/VHD1-A/VHD106Q.Seq.d/                       1398   0.0
VHB613 (VHB613Q) /CSM/VH/VHB6-A/VHB613Q.Seq.d/                       1398   0.0
VHB228 (VHB228Q) /CSM/VH/VHB2-B/VHB228Q.Seq.d/                       1398   0.0
VHA614 (VHA614Q) /CSM/VH/VHA6-A/VHA614Q.Seq.d/                       1398   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.  1241   0.0    2
AF107291|AF107291.1 Candida parapsilosis elongation factor 2 (EFT2) gene, partial cds.    72   6e-71  13
AF107286|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds.    78   2e-70  9
Y09664|Y09664.1 C.albicans EFT2 gene.                              78   4e-69  9
AF107289|AF107289.1 Candida tropicalis elongation factor 2 (EFT2) gene, partial cds.    70   6e-66  10
AF107290|AF107290.1 Candida lusitaniae elongation factor 2 (EFT2) gene, partial cds.    64   5e-49  8
AF534908|AF534908.2 Tetrahymena thermophila elongation factor 2 gene, complete cds.    84   4e-47  6
AY144999|AY144999.1 Saccharomyces kluyveri clone Contig1706 EFT2 gene, complete cds.    78   4e-45  7
AR550596|AR550596.1 Sequence 5727 from patent US 6747137.          78   2e-44  6
EH015431|EH015431.1 USDA-FP_188032 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt081_G08 5', mRNA sequence.    78   6e-43  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   287   7e-76
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   287   7e-76
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   286   1e-75
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   285   3e-75
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   283   8e-75
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   283   1e-74
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   283   1e-74
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   281   5e-74
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   281   5e-74
AK298498_1(AK298498|pid:none) Homo sapiens cDNA FLJ56548 complet...   281   5e-74

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHB715 is cyt

>VHB715F.Seq
GGAACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAAT
TCGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGA
TCACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAA
AGTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCAC
CATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGC
TGGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTT
CAGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTG
CGTCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTAT
CAAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACAC
TGAAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAA
CACTGAAGNNNNNNNNNN

>VHB715Z.Seq
NNNNNNNNNNATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGT
CCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAAT
GAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGT
GATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCC
ATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAA
TTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAA
AACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAA
GAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCC
TTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTC
TTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCT
TTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCAC
GAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAANATAGTAATCAAATCTAA
AAAAAATTTTAAAAAAA

>VHB715P.Seq
GGAACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAAT
TCGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGA
TCACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAA
AGTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCAC
CATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGC
TGGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTT
CAGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTG
CGTCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTAT
CAAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACAC
TGAAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAA
CACTGAAG----------ATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGT
CTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAAT
ATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTG
TTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACA
CTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATG
CTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTG
CCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTA
TTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTAT
TAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCAC
AATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTG
AAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACA
AATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAANATAGTAATC
AAATCTAAAAAAAATTTTAAAAAAA