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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB687
Library VH
(Link to library)
Clone ID VHB687
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHB687P
(Link to Original site)
Representative DNA sequence 
>VHB687 (VHB687Q) /CSM/VH/VHB6-D/VHB687Q.Seq.d/
TACTATTCCATATTTTAAAAATATTAAATAATTAAATATTAAAAATGTCATCAATTGGTA
TTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTA
TTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATACAGAAC
GTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTG
ATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATATGAAAC
ATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGAATTCA
AAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAAAAATGA
AAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGTTCCAG
CTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAAAATTAA
ATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTXXXXXXXXX
XTCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGGACGTGCTATGACCAA
AGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGT
ACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGA
AGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTC
AAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACA
ACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAA
TTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGA
ATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGA
ATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTA
TCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACC
AAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACAT
CTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTCTCATA
TATAGTACACA
sequence update 2002.10.25
Translated Amino Acid sequence 
lfhilkilnn*ILKMSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDTER
LIGDAAKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFK
GETKVFSPEEISSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKLN
VQRIINEPTAAAIAYG---

---SDNQPGVLIQVYEGGRAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNV
SAEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFT
VKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMS
KLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*kiis**nq*s
fsyivh
Translated Amino Acid sequence (All Frames) 
Frame A:
yysif*ky*iiky*kchqlvli*vqhihvlvfgkmivlkllqmikvieplhlmlhlliqn
v*lvmlqriklq*iqpiqylmlnv*lvvnlvtkkfkvi*nighsklfqrmvishifklns
kvklkfshqkkslqwys*k*kkplklilvkpliml*lpfqlismivnvkqpkmlvqfqn*
mfnvllmnqllllllmv---

---SDNQPGVLIQVYEGGRAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNV
SAEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFT
VKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMS
KLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*kiis**nq*s
fsyivh

Frame B:
tipyfknik*lniknvinwy*frynifmcwclak*sc*nyck*sr**nhsilcciy*yrt
fnw*cckesscnesnqysi*c*tfnws*i**qrssk*yetlaiqsyskgw**atyss*iq
r*n*sfltrrnlfngtlknernr*slsw*nh**ccnyrsslfq**stssnqrcwynfkik
cstyy**tncccyclw---

---liinqvy*fkfmkvdvl*pkiiiywvnlnflvfhqhqevyhklklhlmlmqmvs*my
qlkinqlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkmnnkkivlnqrinwkimhsl
lkiqlkmkklqpkfqiqinqllnlklkvysng*nqikpqkrmnmkir*kp*kqlsiqs*v
nsikkevchkvvvcqvvcqmihqrvqttk*tn*idgifqrktslsnrflkk**vskinka
shi*yt

Frame C:
lfhilkilnn*ILKMSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDTER
LIGDAAKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFK
GETKVFSPEEISSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKLN
VQRIINEPTAAAIAYG---

---**strcinssl*rwtcydqr**fig*i*tfwyststkrctts*syi*c*ckwylkci
s*r*inw*qtkdynyq**rssfkrrn*kngc*creiqttr*ttkrsc*ike*igklcihc
*kfn*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*i*r*deslrsscqsnhe*
tlsrrryatrwwyarwyvk*ftkefkqqsrrirlteffrgkhlyqidflknnklvksikl
liyst
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB687 (VHB687Q) /CSM/VH/VHB6-D/VHB687Q.Seq.d/                       2488   0.0
VHP719 (VHP719Q) /CSM/VH/VHP7-A/VHP719Q.Seq.d/                       1439   0.0
VHP716 (VHP716Q) /CSM/VH/VHP7-A/VHP716Q.Seq.d/                       1439   0.0
VHP437 (VHP437Q) /CSM/VH/VHP4-B/VHP437Q.Seq.d/                       1439   0.0
VHO677 (VHO677Q) /CSM/VH/VHO6-D/VHO677Q.Seq.d/                       1439   0.0
VHN681 (VHN681Q) /CSM/VH/VHN6-D/VHN681Q.Seq.d/                       1439   0.0
VHK721 (VHK721Q) /CSM/VH/VHK7-A/VHK721Q.Seq.d/                       1439   0.0
VHJ319 (VHJ319Q) /CSM/VH/VHJ3-A/VHJ319Q.Seq.d/                       1439   0.0
VHD443 (VHD443Q) /CSM/VH/VHD4-B/VHD443Q.Seq.d/                       1439   0.0
VHB518 (VHB518Q) /CSM/VH/VHB5-A/VHB518Q.Seq.d/                       1439   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).  1439   0.0    1
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.  1431   0.0    1
AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.   285   e-166  6
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   285   e-156  8
A58313|A58313.1 Sequence 1 from Patent WO9636707.                 125   e-120  9
Z30210|Z30210.1 C.albicans (ATCC 20955) cahsp70 mRNA for heat shock protein 70.   125   e-120  9
AX536934|AX536934.1 Sequence 535 from Patent WO02064766.          125   e-120  9
AR941672|AR941672.1 Sequence 535 from patent US 7101990.          125   e-120  9
AF221541|AF221541.1 Cryptosporidium serpentis heat shock protein 70 (HSP70) gene, partial cds.   145   e-110  10
M84652|M84652.1 Entamoeba heat shock protein (Hsp70) mRNA, complete cds.   172   e-102  8
dna update 2007. 4. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   365   3e-99
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   363   1e-98
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   341   3e-92
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   341   3e-92
(Q54BE0) RecName: Full=Heat shock cognate 70 kDa protein 3;           323   7e-87
U71151_1(U71151|pid:none) Pneumocystis carinii heat shock protei...   315   2e-84
AY495382_1(AY495382|pid:none) Trypanosoma rangeli isolate Tre he...   315   2e-84
U80967_1(U80967|pid:none) Pneumocystis carinii f. sp. carinii he...   315   2e-84
(P11145) RecName: Full=Heat shock 70 kDa protein 4; AltName: Ful...   314   5e-84
EF442767_1(EF442767|pid:none) Trypanosoma rangeli isolate H14 cl...   313   9e-84
protein update 2009. 6.27
PSORT 

 psg: 0.87  gvh: 0.35  alm: 0.46  top: 0.27  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHB687 is cyt
5' end seq. ID VHB687F
5' end seq. 
>VHB687F.Seq
TACTATTCCATATTTTAAAAATATTAAATAATTAAATATTAAAAATGTCATCAATTGGTA
TTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTA
TTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATACAGAAC
GTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTG
ATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATATGAAAC
ATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGAATTCA
AAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAAAAATGA
AAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGTTCCAG
CTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAAAATTAA
ATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTNNNNNNNNN
N
Length of 5' end seq. 601
3' end seq. ID VHB687Z
3' end seq. 
>VHB687Z.Seq
NNNNNNNNNNTCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGGACGTGC
TATGACCAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACC
AAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGT
ATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGG
TCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACA
AGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCAC
TGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATC
AACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGA
AAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAG
TAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAA
TGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGA
GGAAAACATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAG
CTTCTCATATATAGTACACA
Length of 3' end seq. 740
Connected seq. ID VHB687P
Connected seq. 
>VHB687P.Seq
TACTATTCCATATTTTAAAAATATTAAATAATTAAATATTAAAAATGTCATCAATTGGTA
TTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTA
TTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATACAGAAC
GTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTG
ATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATATGAAAC
ATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGAATTCA
AAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAAAAATGA
AAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGTTCCAG
CTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAAAATTAA
ATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTT---------
-TCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGGACGTGCTATGACCAA
AGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGT
ACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGA
AGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTC
AAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACA
ACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAA
TTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGA
ATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGA
ATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTA
TCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACC
AAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACAT
CTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTCTCATA
TATAGTACACA
Length of connected seq. 1321
Full length Seq ID -
Full length Seq. -
Length of full length seq. -