>VHB675 (VHB675Q) /CSM/VH/VHB6-D/VHB675Q.Seq.d/
TTTATTTTTTTTTTGATCGTTCGAATCAACTCTTGTTGTTTGTTTGAAAATCAAATCAAT
TTCCTTTAAAGATAGTTTTTTAAAGAAAAANGGCNCCACCAACAAAAGAATTAACAAATA
AAGAGATTACAGCAATCAAGGGTATTTCAAAATTATTTGATGATAANAAATACAGTAAAG
GTTTAAAAGAGTGTAATGCAATTTTAAAGAATCATCCAGAAAATCCAGATGCATCATGTT
TTAANAGTTTGATTTTATACAATTTANATCANCAATATGAAGCACGTGAAAATGCAATCA
AAACCACCAAAACCTTACCAACCAACTCAATGGCATGGCATACATTAGGTTACATTTATA
AATTGGATAANAACTATCTCGATGCAACTACAGCCTTTAAAAATGCTTCAAAATTCAATA
AAGAAAGTTCACAAATCTTAAAANAATTANCACCACTTCAATTATATTCAAGANATTTAN
AAGGTTTAAAGGAAACGTATGCANCATTATTAAAATTACAACCAACTCATAANCCACATT
GGACAGGATTAGTGACTTCATATCAATTAATGGGACAAACTAAATCAGCATTATCAGTAT
TTGAAAGXXXXXXXXXXGGAACCAAAGAAAGATCCAAACACTGGTCTCACCATTCCAGAG
GATGACGATTTGAATGGTAAAAAGTTCTTGGTTAAACAAGTTAATCTCATGGATTCAGCT
CAACTCATTTTAGATAATCTCTTGAAAAATACAAATGATATTGAAATTTATCCGTTAGCT
TGCCAAGTTAATACACATCAAAAGAAATATTTATTAGTATTAAAATCATTGGTTAAAATT
AAATCAATTAATGAAAATAGTCCATGTTATTATGAAAATTTAGTTGATTTAATGATTAAA
GTATCAGAAGATAAAGAGGTATCAGCAATTGTTAAAGCAGCAGTTGAAAAACAAGCAGGT
TTTTCATCAGATGTTGAATCAATTTTAGCAAACAACCAAACTTATCTCGAAAAACTTGGT
GACAATGTTACCGCTCAACATCGTTTCGTTGTTGGTAAAATCATTGCCAAACTTTCACCA
TCATCACTTGATAAAGCATCAGAATTGATTTTGAATGTTCAAGGTGAAACAAGTTGGAAA
CAATGCGCTGACAACCTTCAATCTCTTTCTCTTGTATCTTCCGAACTCACTTCTAAATAC
AGAGAAAAATTACACGAACATTTCCCAATTGCTAACTATTTCCAACCAAAAGTTGAAAAT
TATTCAAAATAAAAAAAATAATAATAANAATAATAAANAAATCAATTTAAAAATAACAAT
CAATAA

yfffdrsnqlllfv*ksnqfplkivf*RKXAPPTKELTNKEITAIKGISKLFDDXKYSKG
LKECNAILKNHPENPDASCFXSLILYNLXXQYEARENAIKTTKTLPTNSMAWHTLGYIYK
LDXNYLDATTAFKNASKFNKESSQILKXLXPLQLYSRXLXGLKETYAXLLKLQPTHXPHW
TGLVTSYQLMGQTKSALSVFE---

---EPKKDPNTGLTIPEDDDLNGKKFLVKQVNLMDSAQLILDNLLKNTNDIEIYPLACQV
NTHQKKYLLVLKSLVKIKSINENSPCYYENLVDLMIKVSEDKEVSAIVKAAVEKQAGFSS
DVESILANNQTYLEKLGDNVTAQHRFVVGKIIAKLSPSSLDKASELILNVQGETSWKQCA
DNLQSLSLVSSELTSKYREKLHEHFPIANYFQPKVENYSK*kk***x**xnqfknnnq*

Frame A:
fifflivrinscclfenqinfl*r*ffkekxxhqqkn*qikrlqqsrvfqnylmixntvk
v*ksvmqf*riiqkiqmhhvlxv*fytixixnmkhvkmqskppkpyqptqwhgih*vtfi
nwixtismqlqplkmlqnsikkvhks*xnxhhfnyiqxixkv*rkrmxhy*nynqlixhi
gqd**lhin*wdklnqhyqylk---

---gtkerskhwshhsrg*rfew*kvlg*ts*shgfssthfr*slekyk*y*nlsvslps
*ytskeifisikiig*n*in**k*smll*kfs*fnd*sirr*rgisnc*sss*ktsrffi
rc*infskqpnlsrktw*qcyrstsfrcw*nhcqtftiit**siridfecsr*nkletmr
*qpsisfscifrthf*iqrkitrtfpnc*lfptks*klfkikkiiixiixksi*k*qsi

Frame B:
lfff*sfestlvvclkiksisfkdsflkkxgxtnkrink*rdysnqgyfkii***xiq*r
fkrv*cnfkessrksrcimf*xfdfiqfxsxi*st*kcnqnhqnltnqlngmayirlhl*
ig*xlsrcnysl*kcfkiq*rkftnlkxixttsiifkxfxrfkgnvcxiikittns*xtl
drisdfisingtn*isiisi*k---

---EPKKDPNTGLTIPEDDDLNGKKFLVKQVNLMDSAQLILDNLLKNTNDIEIYPLACQV
NTHQKKYLLVLKSLVKIKSINENSPCYYENLVDLMIKVSEDKEVSAIVKAAVEKQAGFSS
DVESILANNQTYLEKLGDNVTAQHRFVVGKIIAKLSPSSLDKASELILNVQGETSWKQCA
DNLQSLSLVSSELTSKYREKLHEHFPIANYFQPKVENYSK*kk***x**xnqfknnnq*

Frame C:
yfffdrsnqlllfv*ksnqfplkivf*RKXAPPTKELTNKEITAIKGISKLFDDXKYSKG
LKECNAILKNHPENPDASCFXSLILYNLXXQYEARENAIKTTKTLPTNSMAWHTLGYIYK
LDXNYLDATTAFKNASKFNKESSQILKXLXPLQLYSRXLXGLKETYAXLLKLQPTHXPHW
TGLVTSYQLMGQTKSALSVFE---

---nqrkiqtlvspfqrmti*mvksswlnkliswiqlnsf*iis*kiqmilkfir*lakl
ihikrniy*y*nhwlklnqlmkivhvimki*li**lkyqkikryqqllkqqlknkqvfhq
mlnqf*qttklisknlvtmlplnivsllvkslpnfhhhhlikhqn*f*mfkvkqvgnnal
ttfnlfllylpnsllnteknytnisqlltisnqklkiiqnkknnnxnnkxinlkitin

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB675 (VHB675Q) /CSM/VH/VHB6-D/VHB675Q.Seq.d/                       2339   0.0
VHB251 (VHB251Q) /CSM/VH/VHB2-C/VHB251Q.Seq.d/                       1265   0.0
VHO243 (VHO243Q) /CSM/VH/VHO2-B/VHO243Q.Seq.d/                       1263   0.0
VHJ545 (VHJ545Q) /CSM/VH/VHJ5-B/VHJ545Q.Seq.d/                       1263   0.0
VHD223 (VHD223Q) /CSM/VH/VHD2-A/VHD223Q.Seq.d/                       1263   0.0
VHA488 (VHA488Q) /CSM/VH/VHA4-D/VHA488Q.Seq.d/                       1263   0.0
VHA402 (VHA402Q) /CSM/VH/VHA4-A/VHA402Q.Seq.d/                       1263   0.0
VHO636 (VHO636Q) /CSM/VH/VHO6-B/VHO636Q.Seq.d/                       1261   0.0
VHQ454 (VHQ454Q) /CSM/VH/VHQ4-C/VHQ454Q.Seq.d/                       1257   0.0
SHF754 (SHF754Q) /CSM/SH/SHF7-C/SHF754Q.Seq.d/                       1255   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   882   0.0    5
CR457451|CR457451.2 Zebrafish DNA sequence *** SEQUENCING CANCELLED *** from clone  CH211-197B3.    52   0.041  1
BX511270|BX511270.10 Zebrafish DNA sequence from clone DKEY-35N12 in linkage group 14.    52   0.041  1
AE017143|AE017143.1 Haemophilus ducreyi strain 35000HP complete genome.    36   0.085  2
AZ539456|AZ539456.1 ENTDG45TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    38   0.12   2
CN473198|CN473198.1 USDA-FP_122286 Diaprepes abbreviatus, Diaprepes root weevil Diaprepes abbreviatus cDNA clone SLtDaF019_D03, mRNA sequence.    44   0.55   2
CN473197|CN473197.1 USDA-FP_122285 Diaprepes abbreviatus, Diaprepes root weevil Diaprepes abbreviatus cDNA clone SLtDaF019_D03, mRNA sequence.    44   0.55   2
EK086647|EK086647.1 1092961158084 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061001979764 5', genomic survey sequence.    48   0.64   1
EK029168|EK029168.1 1092955394680 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061001979764 3', genomic survey sequence.    48   0.64   1
EJ731506|EJ731506.1 1092960163465 Global-Ocean-Sampling_GS-30-02-01-1P0-1P3KB marine metagenome genomic clone 1061001877297 5', genomic survey sequence.    48   0.64   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116305_76(AC116305|pid:none) Dictyostelium discoideum chromoso...   385   e-105
EU952143_1(EU952143|pid:none) Zea mays clone 1211771 hypothetica...    94   1e-17
AK118131_1(AK118131|pid:none) Arabidopsis thaliana At1g80410 mRN...    93   2e-17
BC142456_1(BC142456|pid:none) Bos taurus NMDA receptor regulated...    91   7e-17
BX640665_1(BX640665|pid:none) Homo sapiens mRNA; cDNA DKFZp686O0...    90   2e-16
(Q6N069) RecName: Full=NMDA receptor-regulated 1-like protein;  ...    90   2e-16
AC018849_25(AC018849|pid:none) Arabidopsis thaliana chromosome 1...    89   5e-16
AY391464_1(AY391464|pid:none) Danio rerio transcriptional coacti...    87   1e-15
BC044392_1(BC044392|pid:none) Danio rerio NMDA receptor-regulate...    87   1e-15
(Q80UM3) RecName: Full=NMDA receptor-regulated protein 1; AltNam...    86   4e-15

 psg: 0.63  gvh: 0.24  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 20.0 %: cytoplasmic
	 16.0 %: mitochondrial
	 12.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHB675 is nuc

>VHB675F.Seq
TTTATTTTTTTTTTGATCGTTCGAATCAACTCTTGTTGTTTGTTTGAAAATCAAATCAAT
TTCCTTTAAAGATAGTTTTTTAAAGAAAAANGGCNCCACCAACAAAAGAATTAACAAATA
AAGAGATTACAGCAATCAAGGGTATTTCAAAATTATTTGATGATAANAAATACAGTAAAG
GTTTAAAAGAGTGTAATGCAATTTTAAAGAATCATCCAGAAAATCCAGATGCATCATGTT
TTAANAGTTTGATTTTATACAATTTANATCANCAATATGAAGCACGTGAAAATGCAATCA
AAACCACCAAAACCTTACCAACCAACTCAATGGCATGGCATACATTAGGTTACATTTATA
AATTGGATAANAACTATCTCGATGCAACTACAGCCTTTAAAAATGCTTCAAAATTCAATA
AAGAAAGTTCACAAATCTTAAAANAATTANCACCACTTCAATTATATTCAAGANATTTAN
AAGGTTTAAAGGAAACGTATGCANCATTATTAAAATTACAACCAACTCATAANCCACATT
GGACAGGATTAGTGACTTCATATCAATTAATGGGACAAACTAAATCAGCATTATCAGTAT
TTGAAAGNNNNNNNNNN

>VHB675Z.Seq
NNNNNNNNNNGGAACCAAAGAAAGATCCAAACACTGGTCTCACCATTCCAGAGGATGACG
ATTTGAATGGTAAAAAGTTCTTGGTTAAACAAGTTAATCTCATGGATTCAGCTCAACTCA
TTTTAGATAATCTCTTGAAAAATACAAATGATATTGAAATTTATCCGTTAGCTTGCCAAG
TTAATACACATCAAAAGAAATATTTATTAGTATTAAAATCATTGGTTAAAATTAAATCAA
TTAATGAAAATAGTCCATGTTATTATGAAAATTTAGTTGATTTAATGATTAAAGTATCAG
AAGATAAAGAGGTATCAGCAATTGTTAAAGCAGCAGTTGAAAAACAAGCAGGTTTTTCAT
CAGATGTTGAATCAATTTTAGCAAACAACCAAACTTATCTCGAAAAACTTGGTGACAATG
TTACCGCTCAACATCGTTTCGTTGTTGGTAAAATCATTGCCAAACTTTCACCATCATCAC
TTGATAAAGCATCAGAATTGATTTTGAATGTTCAAGGTGAAACAAGTTGGAAACAATGCG
CTGACAACCTTCAATCTCTTTCTCTTGTATCTTCCGAACTCACTTCTAAATACAGAGAAA
AATTACACGAACATTTCCCAATTGCTAACTATTTCCAACCAAAAGTTGAAAATTATTCAA
AATAAAAAAAATAATAATAANAATAATAAANAAATCAATTTAAAAATAACAATCAATAA

>VHB675P.Seq
TTTATTTTTTTTTTGATCGTTCGAATCAACTCTTGTTGTTTGTTTGAAAATCAAATCAAT
TTCCTTTAAAGATAGTTTTTTAAAGAAAAANGGCNCCACCAACAAAAGAATTAACAAATA
AAGAGATTACAGCAATCAAGGGTATTTCAAAATTATTTGATGATAANAAATACAGTAAAG
GTTTAAAAGAGTGTAATGCAATTTTAAAGAATCATCCAGAAAATCCAGATGCATCATGTT
TTAANAGTTTGATTTTATACAATTTANATCANCAATATGAAGCACGTGAAAATGCAATCA
AAACCACCAAAACCTTACCAACCAACTCAATGGCATGGCATACATTAGGTTACATTTATA
AATTGGATAANAACTATCTCGATGCAACTACAGCCTTTAAAAATGCTTCAAAATTCAATA
AAGAAAGTTCACAAATCTTAAAANAATTANCACCACTTCAATTATATTCAAGANATTTAN
AAGGTTTAAAGGAAACGTATGCANCATTATTAAAATTACAACCAACTCATAANCCACATT
GGACAGGATTAGTGACTTCATATCAATTAATGGGACAAACTAAATCAGCATTATCAGTAT
TTGAAAG----------GGAACCAAAGAAAGATCCAAACACTGGTCTCACCATTCCAGAG
GATGACGATTTGAATGGTAAAAAGTTCTTGGTTAAACAAGTTAATCTCATGGATTCAGCT
CAACTCATTTTAGATAATCTCTTGAAAAATACAAATGATATTGAAATTTATCCGTTAGCT
TGCCAAGTTAATACACATCAAAAGAAATATTTATTAGTATTAAAATCATTGGTTAAAATT
AAATCAATTAATGAAAATAGTCCATGTTATTATGAAAATTTAGTTGATTTAATGATTAAA
GTATCAGAAGATAAAGAGGTATCAGCAATTGTTAAAGCAGCAGTTGAAAAACAAGCAGGT
TTTTCATCAGATGTTGAATCAATTTTAGCAAACAACCAAACTTATCTCGAAAAACTTGGT
GACAATGTTACCGCTCAACATCGTTTCGTTGTTGGTAAAATCATTGCCAAACTTTCACCA
TCATCACTTGATAAAGCATCAGAATTGATTTTGAATGTTCAAGGTGAAACAAGTTGGAAA
CAATGCGCTGACAACCTTCAATCTCTTTCTCTTGTATCTTCCGAACTCACTTCTAAATAC
AGAGAAAAATTACACGAACATTTCCCAATTGCTAACTATTTCCAACCAAAAGTTGAAAAT
TATTCAAAATAAAAAAAATAATAATAANAATAATAAANAAATCAATTTAAAAATAACAAT
CAATAA