>VHB669 (VHB669Q) /CSM/VH/VHB6-C/VHB669Q.Seq.d/
ATTTTTTTAATTTTTTTTCAAAAAGAGACATAAAAGATGGAAAGTTTAAGTGAACAAGAA
ATAAAGTATAGAGAAGATACAACTAAAGTTTACAGGGAGTTATTATCAATTCCAACAGTC
AACAACGCATTTTTTACAAACAATGATGAAAGTAATGAAAATCAAACATCAGCATCAATC
ATTGTATCTCAAATTGATATCACCAATAAGAAAACTAAATTTTATATGTCACAAAAAACA
ATTTTAAAAGATTCATCATCATCATCAAAGAAAAACCAAGTAATTTCATCAGCAATTCAA
CAAGAATTAGCAACTTCAATCATATCAGTATCACCATCACATAAGAAAGTTTTAACAATT
AAAGAGAACCCTTCAACTGAATTCGAATTCTCTTTTGATATTACTGATGGTTCACATTTA
ATTACTTCAATTACTTCAAAAGATATTCATAAAAAAATTTTAAATGATGAATGGTTTGGT
AGTTTTTCATGGTCACCATGTGAGAAATATGTAGCATTTATTGCAGATTCAAAATTAAAT
GTAACAGGATTTTTTGATAAGGAACXXXXXXXXXXTCTCATTGGGTTATAATATTATAAT
TCCAAATTATAGAGGTTCAACAGGTTATGGTAAAGATTTCGTAGATTGTTTACCAGGTTA
TATTGGTGATTTGGATGTTGACGATTGTGTTCAAGCAATTAATTACACACTCGATAAAAT
CGATAATACAATTGATAGAAATAAAATTGGTGTTATTGGTGGTAGTCATGGTGGTTTCTT
ATCAGCACATCTAAGTAGATTCCCAATTGTCAAAACTTCAATCATGAGAAATCCTGTAAT
CGATATTCCATCAATGTCAACCTTATCCGATATTCCAGATTGGTGTTTCTTTGAAGCTGG
TATTAATCTCTCTGATCCAACCTCTCAATATCATACATTACCATCAATGGAAGAAATTGA
AAAAATGAGAAAATGTTCACCAAGTTTCCATATTGAAAACATCAAAATTCCATCATTATT
AGCTTTAGGTGATTCAGATCTTCGTGTTCCACCTTCTCAAGGTTTATTATATTATCGTAT
GTTAAGAGAAAGAGATGTACCAACAAAATGTTTAATGTATCCAAAAACTGGTCATAGTCT
TGATTCAATTGATGCTCGTTTAGATCAATGGGTTAATATTTCATTATGGTTAAAAAAATA
TTTAAATTAAAATTTTTAAAAAAAATTACAACAATTACACCACCACCACCACCACTACCA
CCACCATATGGTAAAATAATATTATTAAATAAAAACATTT

ifliffqket*KMESLSEQEIKYREDTTKVYRELLSIPTVNNAFFTNNDESNENQTSASI
IVSQIDITNKKTKFYMSQKTILKDSSSSSKKNQVISSAIQQELATSIISVSPSHKKVLTI
KENPSTEFEFSFDITDGSHLITSITSKDIHKKILNDEWFGSFSWSPCEKYVAFIADSKLN
VTGFFDKE---

---SLGYNIIIPNYRGSTGYGKDFVDCLPGYIGDLDVDDCVQAINYTLDKIDNTIDRNKI
GVIGGSHGGFLSAHLSRFPIVKTSIMRNPVIDIPSMSTLSDIPDWCFFEAGINLSDPTSQ
YHTLPSMEEIEKMRKCSPSFHIENIKIPSLLALGDSDLRVPPSQGLLYYRMLRERDVPTK
CLMYPKTGHSLDSIDARLDQWVNISLWLKKYLN*nf*kklqqlhhhhhhyhhhmvk*yy*
iktf

Frame A:
ifliffqket*KMESLSEQEIKYREDTTKVYRELLSIPTVNNAFFTNNDESNENQTSASI
IVSQIDITNKKTKFYMSQKTILKDSSSSSKKNQVISSAIQQELATSIISVSPSHKKVLTI
KENPSTEFEFSFDITDGSHLITSITSKDIHKKILNDEWFGSFSWSPCEKYVAFIADSKLN
VTGFFDKE---

---shwviil*fqiievqqvmvkis*ivyqvilviwmltivfkqlithsiksiiqlieik
lvllvvvmvvsyqhi*vdsqlsklqs*eil*sifhqcqpypifqigvslklvlisliqpl
niihyhqwkklkk*envhqvsilktskfhhy*l*viqifvfhllkvyyiivc*ekemyqq
nv*ciqklvivliqlmlv*inglifhyg*kni*ikifkknynnytttttttttiw*nnii
k*kh

Frame B:
ff*fffkkrhkrwkv*vnkk*siekiqlkftgsyyqfqqstthflqtmmkvmkikhqhqs
lylklispirklnfichkkqf*kihhhhqrktk*fhqqfnkn*qlqsyqyhhhirkf*ql
krtlqlnsnsllillmvhi*llqllqkifikkf*mmnglvvfhghhvrnm*hllqiqn*m
*qdflirn---

---ligl*yynskl*rfnrlw*rfrrlftrlyw*fgc*rlcssn*lhtr*nr*yn**k*n
wcyww*swwflistsk*ipncqnfnhekscnrysinvnlirysrlvfl*swy*sl*snls
isyitingrn*knekmftkfpy*khqnsiiisfr*frsscstfsrfiilsyvkrkrctnk
mfnvsknws*s*fn*csfrsmg*yfimvkkifklkflkkittitppppplpppygkiill
nkni

Frame C:
ffnffskrdikdgkfk*trnkv*rryn*slqgviinsnsqqrifykq**k**ksnisinh
cisn*yhq*en*ilyvtknnfkrfiiiikekpsnfisnstrisnfnhisitit*esfnn*
repfn*irilf*yy*wftfnyfnyfkrys*knfk**mvw*ffmvtm*eicsiycrfkikc
nrif**g---

---SLGYNIIIPNYRGSTGYGKDFVDCLPGYIGDLDVDDCVQAINYTLDKIDNTIDRNKI
GVIGGSHGGFLSAHLSRFPIVKTSIMRNPVIDIPSMSTLSDIPDWCFFEAGINLSDPTSQ
YHTLPSMEEIEKMRKCSPSFHIENIKIPSLLALGDSDLRVPPSQGLLYYRMLRERDVPTK
CLMYPKTGHSLDSIDARLDQWVNISLWLKKYLN*nf*kklqqlhhhhhhyhhhmvk*yy*
iktf

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB669 (VHB669Q) /CSM/VH/VHB6-C/VHB669Q.Seq.d/                       2224   0.0
VHO634 (VHO634Q) /CSM/VH/VHO6-B/VHO634Q.Seq.d/                       1213   0.0
VHO522 (VHO522Q) /CSM/VH/VHO5-A/VHO522Q.Seq.d/                       1213   0.0
VHI656 (VHI656Q) /CSM/VH/VHI6-C/VHI656Q.Seq.d/                       1213   0.0
VHE359 (VHE359Q) /CSM/VH/VHE3-C/VHE359Q.Seq.d/                       1213   0.0
VHD269 (VHD269Q) /CSM/VH/VHD2-C/VHD269Q.Seq.d/                       1213   0.0
VHB485 (VHB485Q) /CSM/VH/VHB4-D/VHB485Q.Seq.d/                       1213   0.0
VHB191 (VHB191Q) /CSM/VH/VHB1-D/VHB191Q.Seq.d/                       1213   0.0
SHC384 (SHC384Q) /CSM/SH/SHC3-D/SHC384Q.Seq.d/                       1213   0.0
AHQ746 (AHQ746Q) /CSM/AH/AHQ7-B/AHQ746Q.Seq.d/                       1213   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

G64996|G64996.1 DH0122 Dictyostelium discoideum ( P.Dear ) Dictyostelium discoideum STS genomic 5', sequence tagged site.   977   0.0    2
AF465304|AF465304.1 Dictyostelium discoideum ABC transporter ABCA.2 (abca2) gene, complete cds.    56   0.003  1
AC116922|AC116922.2 Dictyostelium discoideum chromosome 2 map 6061442-6097630 strain AX4, complete sequence.    36   0.015  6
AL929356|AL929356.1 Plasmodium falciparum strain 3D7, chromosome 9; segment 2/5.    36   0.033  11
AC091416|AC091416.5 Rattus norvegicus clone CH230-2I4, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    36   0.043  7
DT736933|DT736933.1 EST1170782 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1U654, mRNA sequence.    44   0.050  2
DT736695|DT736695.1 EST1170544 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1U516, mRNA sequence.    44   0.053  2
DR922252|DR922252.1 EST1113791 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1MO85, mRNA sequence.    44   0.054  2
DR941098|DR941098.1 EST1132637 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1Q313, mRNA sequence.    44   0.055  2
U23512|U23512.1 Caenorhabditis elegans cosmid M01G4, complete sequence.    42   0.065  5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P13676) RecName: Full=Acylamino-acid-releasing enzyme;         ...   160   9e-38
BC034199_1(BC034199|pid:none) Mus musculus acylpeptide hydrolase...   158   4e-37
BC026594_1(BC026594|pid:none) Mus musculus acylpeptide hydrolase...   158   4e-37
(Q8R146) RecName: Full=Acylamino-acid-releasing enzyme;         ...   158   4e-37
(P19205) RecName: Full=Acylamino-acid-releasing enzyme;         ...   156   1e-36
D38441_1(D38441|pid:none) Human mRNA for acylamino acid-releasin...   155   3e-36
(P80227) RecName: Full=Acylamino-acid-releasing enzyme;         ...   153   1e-35
BC057485_1(BC057485|pid:none) Danio rerio acylpeptide hydrolase,...   152   2e-35
EU972122_1(EU972122|pid:none) Zea mays clone 375508 acylamino-ac...   152   3e-35
AF100669_11(AF100669|pid:none) Caenorhabditis elegans cosmid R11...   151   4e-35

 psg: 0.75  gvh: 0.26  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 40.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for VHB669 is cyt

>VHB669F.Seq
ATTTTTTTAATTTTTTTTCAAAAAGAGACATAAAAGATGGAAAGTTTAAGTGAACAAGAA
ATAAAGTATAGAGAAGATACAACTAAAGTTTACAGGGAGTTATTATCAATTCCAACAGTC
AACAACGCATTTTTTACAAACAATGATGAAAGTAATGAAAATCAAACATCAGCATCAATC
ATTGTATCTCAAATTGATATCACCAATAAGAAAACTAAATTTTATATGTCACAAAAAACA
ATTTTAAAAGATTCATCATCATCATCAAAGAAAAACCAAGTAATTTCATCAGCAATTCAA
CAAGAATTAGCAACTTCAATCATATCAGTATCACCATCACATAAGAAAGTTTTAACAATT
AAAGAGAACCCTTCAACTGAATTCGAATTCTCTTTTGATATTACTGATGGTTCACATTTA
ATTACTTCAATTACTTCAAAAGATATTCATAAAAAAATTTTAAATGATGAATGGTTTGGT
AGTTTTTCATGGTCACCATGTGAGAAATATGTAGCATTTATTGCAGATTCAAAATTAAAT
GTAACAGGATTTTTTGATAAGGAACNNNNNNNNNN

>VHB669Z.Seq
NNNNNNNNNNTCTCATTGGGTTATAATATTATAATTCCAAATTATAGAGGTTCAACAGGT
TATGGTAAAGATTTCGTAGATTGTTTACCAGGTTATATTGGTGATTTGGATGTTGACGAT
TGTGTTCAAGCAATTAATTACACACTCGATAAAATCGATAATACAATTGATAGAAATAAA
ATTGGTGTTATTGGTGGTAGTCATGGTGGTTTCTTATCAGCACATCTAAGTAGATTCCCA
ATTGTCAAAACTTCAATCATGAGAAATCCTGTAATCGATATTCCATCAATGTCAACCTTA
TCCGATATTCCAGATTGGTGTTTCTTTGAAGCTGGTATTAATCTCTCTGATCCAACCTCT
CAATATCATACATTACCATCAATGGAAGAAATTGAAAAAATGAGAAAATGTTCACCAAGT
TTCCATATTGAAAACATCAAAATTCCATCATTATTAGCTTTAGGTGATTCAGATCTTCGT
GTTCCACCTTCTCAAGGTTTATTATATTATCGTATGTTAAGAGAAAGAGATGTACCAACA
AAATGTTTAATGTATCCAAAAACTGGTCATAGTCTTGATTCAATTGATGCTCGTTTAGAT
CAATGGGTTAATATTTCATTATGGTTAAAAAAATATTTAAATTAAAATTTTTAAAAAAAA
TTACAACAATTACACCACCACCACCACCACTACCACCACCATATGGTAAAATAATATTAT
TAAATAAAAACATTT

>VHB669P.Seq
ATTTTTTTAATTTTTTTTCAAAAAGAGACATAAAAGATGGAAAGTTTAAGTGAACAAGAA
ATAAAGTATAGAGAAGATACAACTAAAGTTTACAGGGAGTTATTATCAATTCCAACAGTC
AACAACGCATTTTTTACAAACAATGATGAAAGTAATGAAAATCAAACATCAGCATCAATC
ATTGTATCTCAAATTGATATCACCAATAAGAAAACTAAATTTTATATGTCACAAAAAACA
ATTTTAAAAGATTCATCATCATCATCAAAGAAAAACCAAGTAATTTCATCAGCAATTCAA
CAAGAATTAGCAACTTCAATCATATCAGTATCACCATCACATAAGAAAGTTTTAACAATT
AAAGAGAACCCTTCAACTGAATTCGAATTCTCTTTTGATATTACTGATGGTTCACATTTA
ATTACTTCAATTACTTCAAAAGATATTCATAAAAAAATTTTAAATGATGAATGGTTTGGT
AGTTTTTCATGGTCACCATGTGAGAAATATGTAGCATTTATTGCAGATTCAAAATTAAAT
GTAACAGGATTTTTTGATAAGGAAC----------TCTCATTGGGTTATAATATTATAAT
TCCAAATTATAGAGGTTCAACAGGTTATGGTAAAGATTTCGTAGATTGTTTACCAGGTTA
TATTGGTGATTTGGATGTTGACGATTGTGTTCAAGCAATTAATTACACACTCGATAAAAT
CGATAATACAATTGATAGAAATAAAATTGGTGTTATTGGTGGTAGTCATGGTGGTTTCTT
ATCAGCACATCTAAGTAGATTCCCAATTGTCAAAACTTCAATCATGAGAAATCCTGTAAT
CGATATTCCATCAATGTCAACCTTATCCGATATTCCAGATTGGTGTTTCTTTGAAGCTGG
TATTAATCTCTCTGATCCAACCTCTCAATATCATACATTACCATCAATGGAAGAAATTGA
AAAAATGAGAAAATGTTCACCAAGTTTCCATATTGAAAACATCAAAATTCCATCATTATT
AGCTTTAGGTGATTCAGATCTTCGTGTTCCACCTTCTCAAGGTTTATTATATTATCGTAT
GTTAAGAGAAAGAGATGTACCAACAAAATGTTTAATGTATCCAAAAACTGGTCATAGTCT
TGATTCAATTGATGCTCGTTTAGATCAATGGGTTAATATTTCATTATGGTTAAAAAAATA
TTTAAATTAAAATTTTTAAAAAAAATTACAACAATTACACCACCACCACCACCACTACCA
CCACCATATGGTAAAATAATATTATTAAATAAAAACATTT