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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB661
Library VH
(Link to library)
Clone ID VHB661
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16292-1|Contig-U16400-1
Original site URL
Representative seq. ID VHB661P
(Link to Original site)
Representative DNA sequence 
>VHB661 (VHB661Q) /CSM/VH/VHB6-C/VHB661Q.Seq.d/
TGGCCTACTGGAAAATAAACACTCACTCGATAGTGTAAAAGAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTNAAAAAAAAAXXXXXXXXXXCAAT
CGTTCCCATTCCAAATGATAGTAACATAGAGGCTATCATTGCAACTCTTGAACGTTTTAA
AATCAATGCAATTCTAATGTTTGGTGGATTCAATGGTTATTTGGGCATAGCAAAACTCTA
CGAATATAGAGAGAAATATCAACAACTTAAACGTATCTCAATCATTGGTGCACCTGGTAC
CATTGCAAACAACGTACCAGGTACAAATATCACATTGAATACACTAACCAAGGATTTGGA
AATGTTTGTTGAACGTTTCAAACGTGAACATAGAATTGGTCTAATTATTAAGAGTGAACT
TGCTAGCAACACCTACTCAACTCATTTCATTTATTCACTCTTCAAAGAAGAAGGTAAACA
TTTATTCGATGTCCGTGAATCTATACTCGGTCATTTACAACAAGGTGGTACTCCTTCTGC
AATTGATCGTATCTTTTCAACTCGTTTAATGAATCATTATTATCAATTCTTGGAAAACGA
TCTCAAAGAACATGGTCATCTTCAAATGAATGGTTGTATTGGTTTCATTGATGGTGGTAT
TCATTACACTCCAATGCAAGAAATGATCGAAGAAATGTCTGATAAATTTAGACGTCCTCG
TTCCCAATGGTGGATGGATTTGGTTGAAACCTCTCAAAATATCTCTGTTTTCCCTTTAGA
TGACCCTTCCTCTAT
sequence update 2002.10.25
Translated Amino Acid sequence 
GLLENKHSLDSVKEKKKKKKKKKKKKKKKXFXKKK---

---IVPIPNDSNIEAIIATLERFKINAILMFGGFNGYLGIAKLYEYREKYQQLKRISIIG
APGTIANNVPGTNITLNTLTKDLEMFVERFKREHRIGLIIKSELASNTYSTHFIYSLFKE
EGKHLFDVRESILGHLQQGGTPSAIDRIFSTRLMNHYYQFLENDLKEHGHLQMNGCIGFI
DGGIHYTPMQEMIEEMSDKFRRPRSQWWMDLVETSQNISVFPLDDPSS
Translated Amino Acid sequence (All Frames) 
Frame A:
wptgk*tltr*ckrkkkkkkkkkkkkkkkxffxkk---

---qsfpfqmivt*rlslqllnvlksmqf*clvdsmviwa*qnstnierninnlnvsqsl
vhlvplqttyqvqish*ih*priwkcllnvsnvnielv*llrvnllatptqlisfihssk
kkvniysmsvnlysviynkvvlllqlivsfqlv**iiiinswktisknmvifk*mvvlvs
lmvvfitlqckk*skkclinldvlvpnggwiwlkplkislfsl*mtlpl

Frame B:
GLLENKHSLDSVKEKKKKKKKKKKKKKKKXFXKKK---

---nrshsk***hrgyhcns*tf*nqcnsnvwwiqwlfghsktlri*reistt*tylnhw
ctwyhckqrtrykyhieytnqgfgnvc*tfqt*t*nwsny*e*tc*qhllnsfhlftlqr
rr*tfircp*iytrsfttrwysfcn*sylfnsfnesllsilgkrsqrtwsssnewlywfh
*wwyslhsnarndrrnv**i*tssfpmvdgfg*nlskylcfpfr*pfly

Frame C:
aywkinthsiv*kkkkkkkkkkkkkkkkxxfxkk---

---IVPIPNDSNIEAIIATLERFKINAILMFGGFNGYLGIAKLYEYREKYQQLKRISIIG
APGTIANNVPGTNITLNTLTKDLEMFVERFKREHRIGLIIKSELASNTYSTHFIYSLFKE
EGKHLFDVRESILGHLQQGGTPSAIDRIFSTRLMNHYYQFLENDLKEHGHLQMNGCIGFI
DGGIHYTPMQEMIEEMSDKFRRPRSQWWMDLVETSQNISVFPLDDPSS
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB661 (VHB661Q) /CSM/VH/VHB6-C/VHB661Q.Seq.d/                       1346   0.0
VHB312 (VHB312Q) /CSM/VH/VHB3-A/VHB312Q.Seq.d/                       1292   0.0
CHN469 (CHN469Q) /CSM/CH/CHN4-C/CHN469Q.Seq.d/                       1158   0.0
VFH864 (VFH864Q) /CSM/VF/VFH8-C/VFH864Q.Seq.d/                       1156   0.0
CHP734 (CHP734Q) /CSM/CH/CHP7-B/CHP734Q.Seq.d/                       1088   0.0
CHP390 (CHP390Q) /CSM/CH/CHP3-D/CHP390Q.Seq.d/                       1061   0.0
VHQ114 (VHQ114Q) /CSM/VH/VHQ1-A/VHQ114Q.Seq.d/                       1027   0.0
AHB842 (AHB842Q) /CSM/AH/AHB8-B/AHB842Q.Seq.d/                        938   0.0
VHP708 (VHP708Q) /CSM/VH/VHP7-A/VHP708Q.Seq.d/                        926   0.0
VHP463 (VHP463Q) /CSM/VH/VHP4-C/VHP463Q.Seq.d/                        926   0.0
own update 2002.12.22
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X94340|X94340.1 D.discoideum mRNA for 6-phosphofructo-1-kinase.   926   0.0    2
X89039|X89039.1 D.discoideum mRNA for 6-phosphofructo-1-kinase.   926   0.0    2
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   926   0.0    3
AJ430522|AJ430522.1 Dictyostelium discoideum partial pfk gene for ATP:D-fructose  6-phosphate 1-phosphofructokinase, exon 1.    60   1e-04  1
DX414156|DX414156.1 SOYC248TH LargeInsertSoybeanGenLibBuild4 Glycine max genomic clone H12A17:Build4MTP4H23, genomic survey sequence.    48   0.38   1
CE635221|CE635221.1 tigr-gss-dog-17000366743799 Dog Library Canis familiaris genomic, genomic survey sequence.    46   1.5    1
BZ298846|BZ298846.1 CG4616.f1 Candida glabrata Random Genomic Library Candida glabrata genomic clone CG4616, DNA sequence.    46   1.5    1
BZ297417|BZ297417.1 CG3420.r1 Candida glabrata Random Genomic Library Candida glabrata genomic clone CG3420, DNA sequence.    46   1.5    1
CR380955|CR380955.1 Candida glabrata strain CBS138 chromosome I complete sequence.    46   1.5    1
U97104|U97104.1 Bactrocera tryoni membrane transporter (white) gene, complete cds.    46   1.5    1
dna update 2007. 4. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P90521) RecName: Full=6-phosphofructokinase;          Short=Pho...   339   5e-92
BC081652_1(BC081652|pid:none) Danio rerio phosphofructokinase, m...   120   7e-26
CP001337_2617(CP001337|pid:none) Chloroflexus aggregans DSM 9485...   116   7e-25
A53206(A53206;S03880)6-phosphofructokinase (EC 2.7.1.11) C - rab...   115   2e-24
(P47859) RecName: Full=6-phosphofructokinase type C;          EC...   115   2e-24
AB061205_1(AB061205|pid:none) Gallus gallus pfk mRNA for 6-phosp...   113   6e-24
BC133214_1(BC133214|pid:none) Xenopus laevis hypothetical protei...   112   1e-23
(Q01813) RecName: Full=6-phosphofructokinase type C;          EC...   112   1e-23
BC021698_1(BC021698|pid:none) Homo sapiens, Similar to phosphofr...   112   1e-23
AK092597_1(AK092597|pid:none) Homo sapiens cDNA FLJ35278 fis, cl...   112   1e-23
protein update 2009. 6.27
PSORT 

 psg: 0.75  gvh: 0.35  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.77  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.50
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 88.0 %: nuclear
	  8.0 %: plasma membrane
	  4.0 %: cytoskeletal

>> prediction for VHB661 is nuc
5' end seq. ID VHB661F
5' end seq. 
>VHB661F.Seq
TGGCCTACTGGAAAATAAACACTCACTCGATAGTGTAAAAGAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTNAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 116
3' end seq. ID VHB661Z
3' end seq. 
>VHB661Z.Seq
NNNNNNNNNNCAATCGTTCCCATTCCAAATGATAGTAACATAGAGGCTATCATTGCAACT
CTTGAACGTTTTAAAATCAATGCAATTCTAATGTTTGGTGGATTCAATGGTTATTTGGGC
ATAGCAAAACTCTACGAATATAGAGAGAAATATCAACAACTTAAACGTATCTCAATCATT
GGTGCACCTGGTACCATTGCAAACAACGTACCAGGTACAAATATCACATTGAATACACTA
ACCAAGGATTTGGAAATGTTTGTTGAACGTTTCAAACGTGAACATAGAATTGGTCTAATT
ATTAAGAGTGAACTTGCTAGCAACACCTACTCAACTCATTTCATTTATTCACTCTTCAAA
GAAGAAGGTAAACATTTATTCGATGTCCGTGAATCTATACTCGGTCATTTACAACAAGGT
GGTACTCCTTCTGCAATTGATCGTATCTTTTCAACTCGTTTAATGAATCATTATTATCAA
TTCTTGGAAAACGATCTCAAAGAACATGGTCATCTTCAAATGAATGGTTGTATTGGTTTC
ATTGATGGTGGTATTCATTACACTCCAATGCAAGAAATGATCGAAGAAATGTCTGATAAA
TTTAGACGTCCTCGTTCCCAATGGTGGATGGATTTGGTTGAAACCTCTCAAAATATCTCT
GTTTTCCCTTTAGATGACCCTTCCTCTAT
Length of 3' end seq. 689
Connected seq. ID VHB661P
Connected seq. 
>VHB661P.Seq
TGGCCTACTGGAAAATAAACACTCACTCGATAGTGTAAAAGAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTNAAAAAAAAA----------CAAT
CGTTCCCATTCCAAATGATAGTAACATAGAGGCTATCATTGCAACTCTTGAACGTTTTAA
AATCAATGCAATTCTAATGTTTGGTGGATTCAATGGTTATTTGGGCATAGCAAAACTCTA
CGAATATAGAGAGAAATATCAACAACTTAAACGTATCTCAATCATTGGTGCACCTGGTAC
CATTGCAAACAACGTACCAGGTACAAATATCACATTGAATACACTAACCAAGGATTTGGA
AATGTTTGTTGAACGTTTCAAACGTGAACATAGAATTGGTCTAATTATTAAGAGTGAACT
TGCTAGCAACACCTACTCAACTCATTTCATTTATTCACTCTTCAAAGAAGAAGGTAAACA
TTTATTCGATGTCCGTGAATCTATACTCGGTCATTTACAACAAGGTGGTACTCCTTCTGC
AATTGATCGTATCTTTTCAACTCGTTTAATGAATCATTATTATCAATTCTTGGAAAACGA
TCTCAAAGAACATGGTCATCTTCAAATGAATGGTTGTATTGGTTTCATTGATGGTGGTAT
TCATTACACTCCAATGCAAGAAATGATCGAAGAAATGTCTGATAAATTTAGACGTCCTCG
TTCCCAATGGTGGATGGATTTGGTTGAAACCTCTCAAAATATCTCTGTTTTCCCTTTAGA
TGACCCTTCCTCTAT
Length of connected seq. 785
Full length Seq ID -
Full length Seq. -
Length of full length seq. -