>VHB660 (VHB660Q) /CSM/VH/VHB6-C/VHB660Q.Seq.d/
CATCAACATCAACATCAACATCAACATCAACATCAACATCAACATCAACACCATTACAAA
CACCTACATCACTTGTATCATTATCAAATATTTCATTATCAACCATTTCAATTCCAATTC
AACCAAATACATCATCAATTCTATTAGATAGTAGTATGAACGATAGTTTTAATAAGGAAA
GTAGTGGAAGTAATAAAGTAGCTAACCCAACCATTAGAAAAAGGGATATTATATTAATGA
GTAATTTAGAAGGTATTTGGAATGATATTCCAACAAGATTATATCCATATTTATTAACAT
TGGCCAAGAAATTTAATATAGCTTATCAAATTGATACACTCTATGATCCAACTTCTTGGG
GCTTTGTTTATCCATCACCAATTTCTAATCAACAACAACAACAACAACAACAATCATCAA
CTCAACACCAACACCAACACCACCACCACCAACAACAACAACAAACTAGTATAAATTTAA
ATAGATTATCAATTAGTGGTAGTCCATCATTAAGATCAATTTCAATTCGTAATGGTGGTA
ATATTAATACATTAAATTCAAGTGGATCAAATAATTCAAGTCCATTAAAATTXXXXXXXX
XXAAAGGTATCGCAGCATCTTTACAAACGCTTCATGATAGTAGACCATCAGTGGTTCATC
GTGATTTAAAGAGTCCAAATATCNTACTCTCAAGTAAAGATTCATTAACTATGGAATGCC
ATCTTTGTGATTTCTCATTATCAGGCTTCTCAACCACCGTTGCAAATCGTTCGGTTCAAA
ATCCAGTTTGGTTAGCTCCAGAGGTAATAAACAATGAATTATGTTCGGATAAAAGTGATG
TCTATGCATATGGTGTAATCCTATTTGAACTTTTATCACGTACAAGATTCTTTTCAAATA
TTACCTTTATGTCTGAAGTTGAAGGTTTAATTACCGAGGGTGTACGTCCATCACTACCAT
CTCATAATTTACCAGAGTATGATAGCCTTTTAAATATCTGTTGGGCTCAAGATCCAACTT
GTCGTCCTTCCTTTATTGAAATTACAAAGAAATTAGAAGAAATTGAACTAATCTTAAAAA
CTCATACTCCAGTTGAACCAGTTTACACAGAAACTTCAAAGAATCAACATATTCAATTAA
ATAGAGGAAATACTATATATACCCTTGTTAAAAGACCAATAACACCAATTCAACAACAAC
AACAACAAAAACAACAACAACTACAACAACAAAAACAATCTCCAAAACAATTACAACAAC
AAAAACCATTACCAACTCCTCCAAAACAACTTTCAAATAATGATTCTACTCCAACAAAAC
CATTAGANATTCTAGGNATAGTAGTAGNAAGATTCA

STSTSTSTSTSTSTSTPLQTPTSLVSLSNISLSTISIPIQPNTSSILLDSSMNDSFNKES
SGSNKVANPTIRKRDIILMSNLEGIWNDIPTRLYPYLLTLAKKFNIAYQIDTLYDPTSWG
FVYPSPISNQQQQQQQQSSTQHQHQHHHHQQQQQTSINLNRLSISGSPSLRSISIRNGGN
INTLNSSGSNNSSPLK---

---KGIAASLQTLHDSRPSVVHRDLKSPNIXLSSKDSLTMECHLCDFSLSGFSTTVANRS
VQNPVWLAPEVINNELCSDKSDVYAYGVILFELLSRTRFFSNITFMSEVEGLITEGVRPS
LPSHNLPEYDSLLNICWAQDPTCRPSFIEITKKLEEIELILKTHTPVEPVYTETSKNQHI
QLNRGNTIYTLVKRPITPIQQQQQQKQQQLQQQKQSPKQLQQQKPLPTPPKQLSNNDSTP
TKPLXILGIVVXRF

Frame A:
hqhqhqhqhqhqhqhqhhykhlhhlyhyqifhyqpfqfqfnqihhqfy*ivv*tivlirk
vvevik*ltqplekgily**vi*kvfgmifqqdyihiy*hwprnli*liklihsmiqllg
alfihhqflinnnnnnnnhqlntntnttttnnnnklv*i*idyqlvvvhh*dqfqfvmvv
ilih*iqvdqiiqvh*n---

---KGIAASLQTLHDSRPSVVHRDLKSPNIXLSSKDSLTMECHLCDFSLSGFSTTVANRS
VQNPVWLAPEVINNELCSDKSDVYAYGVILFELLSRTRFFSNITFMSEVEGLITEGVRPS
LPSHNLPEYDSLLNICWAQDPTCRPSFIEITKKLEEIELILKTHTPVEPVYTETSKNQHI
QLNRGNTIYTLVKRPITPIQQQQQQKQQQLQQQKQSPKQLQQQKPLPTPPKQLSNNDSTP
TKPLXILGIVVXRF

Frame B:
inininininininintitntyitciiikyfiinhfnsnstkyiinsir**yer*f**gk
*wk**ss*pnh*kkgyyine*frryle*ysnkiisifinigqei*yslsn*ytl*snflg
lclsitnf*stttttttiinstptptppppttttn*ykfk*iin*w*siikinfns*ww*
y*yikfkwik*fksiki---

---kvsqhlykrfmivdhqwfivi*rvqisysqvkih*lwnaifvishyqasqpplqivr
fkiqfg*lqr**tmnyvrikvmsmhmv*sylnfyhvqdsfqilplclklkv*lprvyvhh
yhliiyqsmiaf*isvglkiqlvvlpllklqrn*kkln*s*klilqlnqftqklqrinif
n*ieeilyipllkdq*hqfnnnnnknnnnynnknnlqnnynnknhyqllqnnfqimillq
qnh*xf*x***xds

Frame C:
STSTSTSTSTSTSTSTPLQTPTSLVSLSNISLSTISIPIQPNTSSILLDSSMNDSFNKES
SGSNKVANPTIRKRDIILMSNLEGIWNDIPTRLYPYLLTLAKKFNIAYQIDTLYDPTSWG
FVYPSPISNQQQQQQQQSSTQHQHQHHHHQQQQQTSINLNRLSISGSPSLRSISIRNGGN
INTLNSSGSNNSSPLK---

---ryrsiftnas***tisgss*fkeskyxtlk*rfinygmpsl*fliirllnhrcksfg
sksslvssrgnkq*imfg*k*clciwcnpi*tfitykilfkyylyv*s*rfnyrgctsit
tis*ftrv**pfkyllgsrsnlssfly*nykeirrn*tnlknsyss*tslhrnfkestys
ik*rkyyiypc*ktnntnstttttktttttttktisktitttktitnsskttfk**fysn
ktirxsrxssxki

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB660 (VHB660Q) /CSM/VH/VHB6-C/VHB660Q.Seq.d/                       1370   0.0
VHE294 (VHE294Q) /CSM/VH/VHE2-D/VHE294Q.Seq.d/                       1160   0.0
VFO465 (VFO465Q) /CSM/VF/VFO4-C/VFO465Q.Seq.d/                       1160   0.0
AHH160 (AHH160Q) /CSM/AH/AHH1-C/AHH160Q.Seq.d/                       1160   0.0
SFD569 (SFD569Q) /CSM/SF/SFD5-C/SFD569Q.Seq.d/                       1136   0.0
VHE712 (VHE712Q) /CSM/VH/VHE7-A/VHE712Q.Seq.d/                        872   0.0
FC-IC1358 (FC-IC1358Q) /CSM/FC-IC/FC-IC1358Q.Seq.d/                   547   e-154
SHC242 (SHC242Q) /CSM/SH/SHC2-B/SHC242Q.Seq.d/                        432   e-120
AHG228 (AHG228Q) /CSM/AH/AHG2-B/AHG228Q.Seq.d/                        188   2e-46
VHJ601 (VHJ601Q) /CSM/VH/VHJ6-A/VHJ601Q.Seq.d/                         56   1e-06

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY232271|AY232271.1 Dictyostelium discoideum putative protein Roco9 (roco9) gene, complete cds.  1160   0.0    1
AY232268|AY232268.1 Dictyostelium discoideum putative protein Roco6 (roco6) gene, complete cds.    56   0.003  1
EJ669762|EJ669762.1 1092955054168 Global-Ocean-Sampling_GS-30-02-01-1P0-1P3KB marine metagenome genomic clone 1061001835384 5', genomic survey sequence.    38   0.033  2
EJ670893|EJ670893.1 1092955057048 Global-Ocean-Sampling_GS-30-02-01-1P0-1P3KB marine metagenome genomic clone 1061001835384 3', genomic survey sequence.    38   0.033  2
EK096404|EK096404.1 1092962039889 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061002054702 3', genomic survey sequence.    38   0.034  2
CQ605966|CQ605966.1 Sequence 33724 from Patent WO0171042.          48   0.65   1
AC017833|AC017833.1 Drosophila melanogaster, WORKING DRAFT SEQUENCE, in ordered pieces.    48   0.65   1
AC006497|AC006497.5 Drosophila melanogaster chromosome 3 clone BACR48B15 (D548) RPCI-98 48.B.15 map 76A3-B4 strain y; cn bw sp, *** SEQUENCING IN PROGRESS ***, 84 unordered pieces.    48   0.65   1
BH166980|BH166980.1 ENTSZ33TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    48   0.65   1
BH166449|BH166449.1 ENTTS69TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    48   0.65   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q6XHA7) RecName: Full=Probable serine/threonine-protein kinase ...   371   e-101
(Q55E58) RecName: Full=Probable serine/threonine-protein kinase ...   124   6e-27
AY170918_1(AY170918|pid:none) Dictyostelium discoideum pats1 gen...   124   6e-27
(Q8MVR1) RecName: Full=Cyclic GMP-binding protein C;          EC...   108   6e-22
EF146477_1(EF146477|pid:none) Populus trichocarpa clone WS0118_H...   105   3e-21
(Q1ZXD6) RecName: Full=Probable serine/threonine-protein kinase ...   105   3e-21
AP005558_11(AP005558|pid:none) Oryza sativa Japonica Group genom...   100   2e-19
FB918162_1(FB918162|pid:none) Sequence 137435 from Patent WO2008...   100   2e-19
BT066283_1(BT066283|pid:none) Zea mays full-length cDNA clone ZM...   100   2e-19
BT033593_1(BT033593|pid:none) Zea mays full-length cDNA clone ZM...   100   2e-19

 psg: 0.87  gvh: 0.58  alm: 0.39  top: 0.33  tms: 0.00  mit: 0.58  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: mitochondrial
	 16.0 %: extracellular, including cell wall
	 16.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: endoplasmic reticulum

>> prediction for VHB660 is mit

>VHB660F.Seq
CATCAACATCAACATCAACATCAACATCAACATCAACATCAACATCAACACCATTACAAA
CACCTACATCACTTGTATCATTATCAAATATTTCATTATCAACCATTTCAATTCCAATTC
AACCAAATACATCATCAATTCTATTAGATAGTAGTATGAACGATAGTTTTAATAAGGAAA
GTAGTGGAAGTAATAAAGTAGCTAACCCAACCATTAGAAAAAGGGATATTATATTAATGA
GTAATTTAGAAGGTATTTGGAATGATATTCCAACAAGATTATATCCATATTTATTAACAT
TGGCCAAGAAATTTAATATAGCTTATCAAATTGATACACTCTATGATCCAACTTCTTGGG
GCTTTGTTTATCCATCACCAATTTCTAATCAACAACAACAACAACAACAACAATCATCAA
CTCAACACCAACACCAACACCACCACCACCAACAACAACAACAAACTAGTATAAATTTAA
ATAGATTATCAATTAGTGGTAGTCCATCATTAAGATCAATTTCAATTCGTAATGGTGGTA
ATATTAATACATTAAATTCAAGTGGATCAAATAATTCAAGTCCATTAAAATTNNNNNNNN
NN

>VHB660Z.Seq
NNNNNNNNNNAAAGGTATCGCAGCATCTTTACAAACGCTTCATGATAGTAGACCATCAGT
GGTTCATCGTGATTTAAAGAGTCCAAATATCNTACTCTCAAGTAAAGATTCATTAACTAT
GGAATGCCATCTTTGTGATTTCTCATTATCAGGCTTCTCAACCACCGTTGCAAATCGTTC
GGTTCAAAATCCAGTTTGGTTAGCTCCAGAGGTAATAAACAATGAATTATGTTCGGATAA
AAGTGATGTCTATGCATATGGTGTAATCCTATTTGAACTTTTATCACGTACAAGATTCTT
TTCAAATATTACCTTTATGTCTGAAGTTGAAGGTTTAATTACCGAGGGTGTACGTCCATC
ACTACCATCTCATAATTTACCAGAGTATGATAGCCTTTTAAATATCTGTTGGGCTCAAGA
TCCAACTTGTCGTCCTTCCTTTATTGAAATTACAAAGAAATTAGAAGAAATTGAACTAAT
CTTAAAAACTCATACTCCAGTTGAACCAGTTTACACAGAAACTTCAAAGAATCAACATAT
TCAATTAAATAGAGGAAATACTATATATACCCTTGTTAAAAGACCAATAACACCAATTCA
ACAACAACAACAACAAAAACAACAACAACTACAACAACAAAAACAATCTCCAAAACAATT
ACAACAACAAAAACCATTACCAACTCCTCCAAAACAACTTTCAAATAATGATTCTACTCC
AACAAAACCATTAGANATTCTAGGNATAGTAGTAGNAAGATTCA

>VHB660P.Seq
CATCAACATCAACATCAACATCAACATCAACATCAACATCAACATCAACACCATTACAAA
CACCTACATCACTTGTATCATTATCAAATATTTCATTATCAACCATTTCAATTCCAATTC
AACCAAATACATCATCAATTCTATTAGATAGTAGTATGAACGATAGTTTTAATAAGGAAA
GTAGTGGAAGTAATAAAGTAGCTAACCCAACCATTAGAAAAAGGGATATTATATTAATGA
GTAATTTAGAAGGTATTTGGAATGATATTCCAACAAGATTATATCCATATTTATTAACAT
TGGCCAAGAAATTTAATATAGCTTATCAAATTGATACACTCTATGATCCAACTTCTTGGG
GCTTTGTTTATCCATCACCAATTTCTAATCAACAACAACAACAACAACAACAATCATCAA
CTCAACACCAACACCAACACCACCACCACCAACAACAACAACAAACTAGTATAAATTTAA
ATAGATTATCAATTAGTGGTAGTCCATCATTAAGATCAATTTCAATTCGTAATGGTGGTA
ATATTAATACATTAAATTCAAGTGGATCAAATAATTCAAGTCCATTAAAATT--------
--AAAGGTATCGCAGCATCTTTACAAACGCTTCATGATAGTAGACCATCAGTGGTTCATC
GTGATTTAAAGAGTCCAAATATCNTACTCTCAAGTAAAGATTCATTAACTATGGAATGCC
ATCTTTGTGATTTCTCATTATCAGGCTTCTCAACCACCGTTGCAAATCGTTCGGTTCAAA
ATCCAGTTTGGTTAGCTCCAGAGGTAATAAACAATGAATTATGTTCGGATAAAAGTGATG
TCTATGCATATGGTGTAATCCTATTTGAACTTTTATCACGTACAAGATTCTTTTCAAATA
TTACCTTTATGTCTGAAGTTGAAGGTTTAATTACCGAGGGTGTACGTCCATCACTACCAT
CTCATAATTTACCAGAGTATGATAGCCTTTTAAATATCTGTTGGGCTCAAGATCCAACTT
GTCGTCCTTCCTTTATTGAAATTACAAAGAAATTAGAAGAAATTGAACTAATCTTAAAAA
CTCATACTCCAGTTGAACCAGTTTACACAGAAACTTCAAAGAATCAACATATTCAATTAA
ATAGAGGAAATACTATATATACCCTTGTTAAAAGACCAATAACACCAATTCAACAACAAC
AACAACAAAAACAACAACAACTACAACAACAAAAACAATCTCCAAAACAATTACAACAAC
AAAAACCATTACCAACTCCTCCAAAACAACTTTCAAATAATGATTCTACTCCAACAAAAC
CATTAGANATTCTAGGNATAGTAGTAGNAAGATTCA