>VHB644 (VHB644Q) /CSM/VH/VHB6-B/VHB644Q.Seq.d/
CATTTCAATGAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATGAAAACT
ATTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCAACATAT
GGAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAAAAACCA
CGTACTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATTTATCCAAAGAAA
CAATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTTGTTGATAATTTA
ATGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATTACAACA
TTAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCTAAAACT
TATTATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCAGGTGATATTCAT
CATATTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACCATTGTTATCGAA
GGTAGTCCAAATAAGATTCCAATGATTTATGGTXXXXXXXXXXCCAACNTTTTCATGGGT
AATGTAAATCCATNTGGTCGTCTTCCTTTAACTTATCCAGGTACTACTGGTGATATCGGT
GTTCCATATTACCACAAATACAGTGAAAATGGNGTCNCCACTCCACTNTTCCAATTTGGT
GATGGTNTTTTTTATACCACTTTTAACTATACCAATNTCGCCTGTAGCAATTGTAAACCA
ATNTTTGGTCAATNTGGTAACTACNCTGGTGTTTTAGGTCAATCCTATACTTTCACTGTC
ACTGTCACAAACAATGGTAACGTTCAAGGTAAAGACTCTGTTNTCTTATATCTC

HFNEYLIKFNFIYIYKKMKTIKSLFLLSLLIVNLLISSTYGSSIRVSIVGGEEAEVIEKP
RTFGNKRELKLEYSQIYPKKQLNQENINFMSARDTFVDNLMSKMSITEKIGQMTQLDITT
LTSPNTITINETTLAYYAKTYYIGSYLNSPVSGGLAGDIHHINSSVWLDMINTIQTIVIE
GSPNKIPMIYG---

---NXFMGNVNPXGRLPLTYPGTTGDIGVPYYHKYSENGVXTPLFQFGDGXFYTTFNYTN
XACSNCKPXFGQXGNYXGVLGQSYTFTVTVTNNGNVQGKDSVXLYL

Frame A:
HFNEYLIKFNFIYIYKKMKTIKSLFLLSLLIVNLLISSTYGSSIRVSIVGGEEAEVIEKP
RTFGNKRELKLEYSQIYPKKQLNQENINFMSARDTFVDNLMSKMSITEKIGQMTQLDITT
LTSPNTITINETTLAYYAKTYYIGSYLNSPVSGGLAGDIHHINSSVWLDMINTIQTIVIE
GSPNKIPMIYG---

---ptfswvm*ihxvvfl*liqvllvisvfhittntvkmxsplhxsnlvmvffiplltip
xspvaivnqxlvnxvttlvf*vnpilslslsqtmvtfkvktlxsyi

Frame B:
ismni*lnsilyiyikr*kllkvyfychc***iy**vqhmevqle*allevkklkslknh
vlleikensn*sivkfiqrnn*ikkilisclpeilllii*cqkcqslkklvk*lnwilqh
*hhqiqlqlmkqh*himlkliildhi*ihqyqvv*qvifiilivvfg*i*lilfkpllsk
vvqirfq*fm---

---qxfhg*cksxwsssfnlsryyw*yrcsilpqiq*kwxxhstxpiw*wxflyhf*lyq
xrl*ql*tnxwsxw*lxwcfrsilyfhchchkqw*rsr*rlcxlis

Frame C:
fq*ifn*iqfyiyi*kdeny*kfiftvivdsefinkfniwkfn*skhcwr*rs*sh*ktt
yfwk*krtqirv*snlsketiksrky*fhvcqryfc**fnvknvnh*knwsndsigyyni
nitkynyn**nnisilc*nllywiifkftsirwfsr*yssy***clvryd*yysnhcyrr
*sk*dsndlw---

---NXFMGNVNPXGRLPLTYPGTTGDIGVPYYHKYSENGVXTPLFQFGDGXFYTTFNYTN
XACSNCKPXFGQXGNYXGVLGQSYTFTVTVTNNGNVQGKDSVXLYL

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ110 (VHQ110Q) /CSM/VH/VHQ1-A/VHQ110Q.Seq.d/                       1045   0.0
VHO350 (VHO350Q) /CSM/VH/VHO3-C/VHO350Q.Seq.d/                       1045   0.0
VHN170 (VHN170Q) /CSM/VH/VHN1-C/VHN170Q.Seq.d/                       1045   0.0
VHM744 (VHM744Q) /CSM/VH/VHM7-B/VHM744Q.Seq.d/                       1045   0.0
VHL744 (VHL744Q) /CSM/VH/VHL7-B/VHL744Q.Seq.d/                       1045   0.0
VHK718 (VHK718Q) /CSM/VH/VHK7-A/VHK718Q.Seq.d/                       1045   0.0
VHK201 (VHK201Q) /CSM/VH/VHK2-A/VHK201Q.Seq.d/                       1045   0.0
VHJ550 (VHJ550Q) /CSM/VH/VHJ5-C/VHJ550Q.Seq.d/                       1045   0.0
VHJ486 (VHJ486Q) /CSM/VH/VHJ4-D/VHJ486Q.Seq.d/                       1045   0.0
VHI201 (VHI201Q) /CSM/VH/VHI2-A/VHI201Q.Seq.d/                       1045   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L21014|L21014.1 Dictyostelium discoideum beta-glucosidase mRNA, complete cds.  1045   0.0    4
EH013950|EH013950.1 USDA-FP_186699 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt064_C10 5', mRNA sequence.    40   0.018  2
BG139849|BG139849.1 EST480291 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP14B10 5' sequence, mRNA sequence.    48   0.062  2
CN215175|CN215175.1 28949 Suspension culture Solanum tuberosum cDNA, mRNA sequence.    40   0.10   3
BX293980|BX293980.2 Mycoplasma mycoides subsp. mycoides SC str. PG1, complete genome.    32   0.17   13
AC163188|AC163188.2 Bos taurus clone CH240-125O20, *** SEQUENCING IN PROGRESS ***, 10 unordered pieces.    44   0.26   3
BQ969550|BQ969550.1 QHB38N02.yg.ab1 QH_ABCDI sunflower RHA801 Helianthus annuus cDNA clone QHB38N02, mRNA sequence.    48   0.43   1
AC073286|AC073286.5 Homo sapiens chromosome 3 clone RP11-298M2, *** SEQUENCING IN PROGRESS ***, 47 unordered pieces.    48   0.43   1
AC067915|AC067915.2 Homo sapiens clone RP11-131G15, WORKING DRAFT SEQUENCE, 21 unordered pieces.    48   0.43   1
AC022656|AC022656.3 Homo sapiens clone RP11-28P11, WORKING DRAFT SEQUENCE, 22 unordered pieces.    48   0.43   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A49881(A49881)beta-glucosidase (EC 3.2.1.21) precursor, lysosoma...   305   1e-81
CP000383_2239(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...    56   1e-06
CP000388_3699(CP000388|pid:none) Pseudoalteromonas atlantica T6c...    55   2e-06
CP000083_2301(CP000083|pid:none) Colwellia psychrerythraea 34H, ...    54   9e-06
Z56279_3(Z56279|pid:none) T.brockii cglF, cglG, xglS and cglT ge...    52   3e-05
AF135015_2(AF135015|pid:none) Thermoanaerobacter ethanolicus put...    52   3e-05
CP000924_2006(CP000924|pid:none) Thermoanaerobacter pseudethanol...    52   3e-05
AY335818_1(AY335818|pid:none) Gossypium hirsutum beta-D-glucosid...    51   5e-05
CP001251_792(CP001251|pid:none) Dictyoglomus turgidum DSM 6724, ...    50   8e-05
AL133292_9(AL133292|pid:none) Arabidopsis thaliana DNA chromosom...    50   1e-04

 psg: 0.74  gvh: 0.73  alm: 0.26  top: 0.07  tms: 0.07  mit: 0.34  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.75  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	 20.0 %: endoplasmic reticulum
	 16.0 %: Golgi
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VHB644 is cyt

>VHB644F.Seq
CATTTCAATGAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATGAAAACT
ATTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCAACATAT
GGAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAAAAACCA
CGTACTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATTTATCCAAAGAAA
CAATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTTGTTGATAATTTA
ATGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATTACAACA
TTAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCTAAAACT
TATTATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCAGGTGATATTCAT
CATATTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACCATTGTTATCGAA
GGTAGTCCAAATAAGATTCCAATGATTTATGGTNNNNNNNNNN

>VHB644Z.Seq
NNNNNNNNNNCCAACNTTTTCATGGGTAATGTAAATCCATNTGGTCGTCTTCCTTTAACT
TATCCAGGTACTACTGGTGATATCGGTGTTCCATATTACCACAAATACAGTGAAAATGGN
GTCNCCACTCCACTNTTCCAATTTGGTGATGGTNTTTTTTATACCACTTTTAACTATACC
AATNTCGCCTGTAGCAATTGTAAACCAATNTTTGGTCAATNTGGTAACTACNCTGGTGTT
TTAGGTCAATCCTATACTTTCACTGTCACTGTCACAAACAATGGTAACGTTCAAGGTAAA
GACTCTGTTNTCTTATATCTC

>VHB644P.Seq
CATTTCAATGAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATGAAAACT
ATTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCAACATAT
GGAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAAAAACCA
CGTACTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATTTATCCAAAGAAA
CAATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTTGTTGATAATTTA
ATGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATTACAACA
TTAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCTAAAACT
TATTATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCAGGTGATATTCAT
CATATTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACCATTGTTATCGAA
GGTAGTCCAAATAAGATTCCAATGATTTATGGT----------CCAACNTTTTCATGGGT
AATGTAAATCCATNTGGTCGTCTTCCTTTAACTTATCCAGGTACTACTGGTGATATCGGT
GTTCCATATTACCACAAATACAGTGAAAATGGNGTCNCCACTCCACTNTTCCAATTTGGT
GATGGTNTTTTTTATACCACTTTTAACTATACCAATNTCGCCTGTAGCAATTGTAAACCA
ATNTTTGGTCAATNTGGTAACTACNCTGGTGTTTTAGGTCAATCCTATACTTTCACTGTC
ACTGTCACAAACAATGGTAACGTTCAAGGTAAAGACTCTGTTNTCTTATATCTC