NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB640
Library VH
(Link to library)
Clone ID VHB640
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16486-1
Original site URL
Representative seq. ID VHB640P
(Link to Original site)
Representative DNA sequence 
>VHB640 (VHB640Q) /CSM/VH/VHB6-B/VHB640Q.Seq.d/
AGAATATTTTAGATTAAAAAACAAAATGAGAGTTTTGTTACTTTTAGTTTTCCTCGTATT
TTGTATCATTCCAGCAGCTCTCTCATTCAGAGACACCAACTGGAGCAAATACAACAGAGC
TGGTAAAAAAGAAACTGTAGATTTCCGTATTGCACTCAATCAAAGAAATTTAGATGTCTT
AGAAAATACTTTATTGGATGTTTCTAACCCAGAATCACCAAATTATGGTAAACATTGGAC
TTCTGAACAAGTTGCTGATTTAGTTGCACCAGAACCATACGTTTCAAAGAAAGTTGCAAA
TTATTTAAAAAAGAACGGTTGTCACAATGTTGAAAATCATCGTGATTACATTAAAGCAAC
TGGTCCAGTTGATTCAGTTGAAAAGATTTTCTCAGTTGAAATGTACAATTACAAACATAA
TATTAAATCCGATCAAGCTATCATTCGTTCATCAGAAACCTATCAAGTTCCATTTGAAAT
CTCTCAATATGTTCACATTGTTTCTGGTATCTCTGAACTCCCACATCTCAAGAGTGGTCC
AAAATCACAAAGATTAAGACCAXXXXXXXXXXCTTCAGGTGATCAAGGTGCCCCAGGTGA
TGGTAATCCATCATGTACCTCAAGAGATCAACCAATCTCAACCATTTTCCCAGGTGCCTC
CCCCATGGGTTACCTCTGTTGGTGCTACCATGTTAGCTGCCCCATCCACCAGCAATCCAA
ATCCAATCGATACTTCTGATGCCCCAGTCTGTGGTATCCACGCTTGTGCTACCTCAACTC
AAGAAGTTACCTGTTCATCTCCATCCGCTTTGATCACTACTGGTGGTGGTTTCTCCAACT
ACTCCCCACAACCAGCATGGCAATCAAAAGCTGTCAATGCTTACCTCAACTCTGGTGTTG
TCTTACCAAACAAAAAATTCTTTAACGCTTCAAACCGTGGTTTCCCAGATGTCTCTGGTT
TAGGTCACAACTACTACATTGTTGCCGCCGATCAAATGAACTTAGTCGATGGTACCTCTT
GTTCATCACCAGTCATTGGTGGTATTGTTGCCTTATTAAACAGCTATCGTTTAGATAATG
GTAAATCAACCCTCGGTTTCATTAACCCAGTCTTATATGCTGCTTACGCCTCTAACCCAG
CTGCCTTCAATGATATCACCGTTGGTAACAATTATTGTACTGAAAGCTGTTGTGCTACCA
CTGGTTTCGTTGCCACCAAAGGTTGGGATGCTGTCACTGGTTTAGGTTCACCAAATTTCA
AACCATTATTATCATACATTCAATCTTTAAATTAAATAAATAAAATTATTTAATAATAAA
AATAAAATAATAATATTATCTAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
EYFRLKNKMRVLLLLVFLVFCIIPAALSFRDTNWSKYNRAGKKETVDFRIALNQRNLDVL
ENTLLDVSNPESPNYGKHWTSEQVADLVAPEPYVSKKVANYLKKNGCHNVENHRDYIKAT
GPVDSVEKIFSVEMYNYKHNIKSDQAIIRSSETYQVPFEISQYVHIVSGISELPHLKSGP
KSQRLRP---

---LQVIKVPQVMVIHHVPQEINQSQPFSQVPPPWVTSVGATMLAAPSTSNPNPIDTSDA
PVCGIHACATSTQEVTCSSPSALITTGGGFSNYSPQPAWQSKAVNAYLNSGVVLPNKKFF
NASNRGFPDVSGLGHNYYIVAADQMNLVDGTSCSSPVIGGIVALLNSYRLDNGKSTLGFI
NPVLYAAYASNPAAFNDITVGNNYCTESCCATTGFVATKGWDAVTGLGSPNFKPLLSYIQ
SLN*inkii***k*nnnii*k
Translated Amino Acid sequence (All Frames) 
Frame A:
rif*ikkqnesfvtfsfprilyhssssliqrhqleqiqqsw*krncrfpyctqskkfrcl
rkyfigcf*pritklw*tldf*tsc*fsctrtirfkescklfkkerlsqc*kss*lh*sn
wss*fs*kdfls*nvqlqt*y*irssyhsfirnlsssi*nlsicshcfwyl*tptsqews
kitkikt---

---LQVIKVPQVMVIHHVPQEINQSQPFSQVPPPWVTSVGATMLAAPSTSNPNPIDTSDA
PVCGIHACATSTQEVTCSSPSALITTGGGFSNYSPQPAWQSKAVNAYLNSGVVLPNKKFF
NASNRGFPDVSGLGHNYYIVAADQMNLVDGTSCSSPVIGGIVALLNSYRLDNGKSTLGFI
NPVLYAAYASNPAAFNDITVGNNYCTESCCATTGFVATKGWDAVTGLGSPNFKPLLSYIQ
SLN*inkii***k*nnnii*k

Frame B:
EYFRLKNKMRVLLLLVFLVFCIIPAALSFRDTNWSKYNRAGKKETVDFRIALNQRNLDVL
ENTLLDVSNPESPNYGKHWTSEQVADLVAPEPYVSKKVANYLKKNGCHNVENHRDYIKAT
GPVDSVEKIFSVEMYNYKHNIKSDQAIIRSSETYQVPFEISQYVHIVSGISELPHLKSGP
KSQRLRP---

---fr*srcpr*w*simylkrstnlnhfprclphglpllvlpc*lphppaiqiqsillmp
qsvvstlvlpqlkklpvhlhpl*sllvvvspttphnqhgnqklsmltstlvlsyqtknsl
tlqtvvsqmslv*vttttllppik*t*smvplvhhqslvvllpy*taiv*imvnqpsvsl
tqsymlltpltqlpsmisplvtiivlkavvlplvslppkvgmlslv*vhqisnhyyhtfn
l*ik*iklfnnknkiiilsk

Frame C:
nild*ktk*efcyf*fssyfvsfqqlshsetptganttelvkkkl*isvlhsikei*ms*
kilywmfltqnhqimvnigllnklli*lhqnhtfqrklqii*krtvvtmlkiivitlkql
vqliqlkrfsqlkctitniilnpiklsfvhqkpikfhlkslnmftlflvslnshisrvvq
nhkd*d---

---sgdqgapgdgnpsctsrdqpistifpgaspmgylcwcyhvscpihqqsksnryf*cp
slwyprlcylnsrsylfisirfdhywwwflqllpttsmaikscqclpqlwccltkqkil*
rfkpwfprclwfrsqllhccrrsnelsrwyllfitshwwycclikqlsfr*w*inprfh*
pslicclrl*psclq*yhrw*qlly*kllcyhwfrchqrlgcchwfrftkfqtiiiihsi
fklnk*nyliikik**yylk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB640 (VHB640Q) /CSM/VH/VHB6-B/VHB640Q.Seq.d/                       2490   0.0
VSD867 (VSD867Q) /CSM/VS/VSD8-C/VSD867Q.Seq.d/                       1400   0.0
FC-BB24 (FC-BB24Q) /CSM/FC/FC-BB/FC-BB24Q.Seq.d/                     1400   0.0
VHH845 (VHH845Q) /CSM/VH/VHH8-B/VHH845Q.Seq.d/                       1398   0.0
VHK260 (VHK260Q) /CSM/VH/VHK2-C/VHK260Q.Seq.d/                       1396   0.0
VHB560 (VHB560Q) /CSM/VH/VHB5-C/VHB560Q.Seq.d/                       1394   0.0
VHQ391 (VHQ391Q) /CSM/VH/VHQ3-D/VHQ391Q.Seq.d/                       1388   0.0
VHA629 (VHA629Q) /CSM/VH/VHA6-B/VHA629Q.Seq.d/                       1386   0.0
VHK807 (VHK807Q) /CSM/VH/VHK8-A/VHK807Q.Seq.d/                       1366   0.0
VHB205 (VHB205Q) /CSM/VH/VHB2-A/VHB205Q.Seq.d/                       1360   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

EC761285|EC761285.1 PSE00007347 rw_mgpallid Polysphondylium pallidum cDNA, mRNA sequence.    66   2e-31  6
EC762663|EC762663.1 PSE00007726 rw_mgpallid Polysphondylium pallidum cDNA, mRNA sequence.    66   3e-22  3
EC761294|EC761294.1 PSE00003790 rw_mgpallid Polysphondylium pallidum cDNA, mRNA sequence.    66   5e-12  2
EC854298|EC854298.1 HDE00000178 Hyperamoeba dachnaya Non-normalized (Amplicon Express) Hyperamoeba dachnaya cDNA, mRNA sequence.    48   5e-08  3
EC854617|EC854617.1 HDE00000592 Hyperamoeba dachnaya Non-normalized (Amplicon Express) Hyperamoeba dachnaya cDNA, mRNA sequence.    48   6e-08  3
EC130556|EC130556.1 HVE00010321 Hartmannella vermiformis Normalized long fraction Hartmannella vermiformis cDNA, mRNA sequence.    46   2e-05  3
U27540|U27540.1 Dictyostelium discoideum vegetative stage specific V4-7 mRNA, partial cds.    40   2e-04  4
CZ858702|CZ858702.1 OC__Ba0250N03.f OC__Ba Oryza coarctata genomic clone OC__Ba0250N03 5', genomic survey sequence.    38   0.039  3
CZ712823|CZ712823.1 OC__Ba0035D04.r OC__Ba Oryza coarctata genomic clone OC__Ba0035D04 3', genomic survey sequence.    38   0.039  3
AM483988|AM483988.1 Vitis vinifera, whole genome shotgun sequence, contig VV78X181058.7, clone ENTAV 115.    44   0.33   4
dna update 2007. 4. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q8MZS4) RecName: Full=Physarolisin;          EC=3.4.21.103; Alt...   251   5e-65
DQ242648_1(DQ242648|pid:none) Phanerochaete chrysosporium family...   144   7e-33
BC135587_1(BC135587|pid:none) Xenopus tropicalis hypothetical pr...   136   2e-30
BC068900_1(BC068900|pid:none) Xenopus laevis hypothetical protei...   132   3e-29
AC116960_13(AC116960|pid:none) Dictyostelium discoideum chromoso...   132   4e-29
(Q9EQV6) RecName: Full=Tripeptidyl-peptidase 1;          Short=T...   129   3e-28
AM411678_1(AM411678|pid:none) Plasmodiophora brassicae ORF1 for ...   128   4e-28
U27540_1(U27540|pid:none) Dictyostelium discoideum vegetative st...   128   5e-28
AB276124_1(AB276124|pid:none) Actinophrys sol mRNA for sedolisin...   127   7e-28
AM920437_1911(AM920437|pid:none) Penicillium chrysogenum Wiscons...   127   7e-28
protein update 2009. 6.27
PSORT 

 psg: 1.00  gvh: 0.75  alm: 0.42  top: 0.47  tms: 0.00  mit: 0.30  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: extracellular, including cell wall
	  8.0 %: mitochondrial
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoplasmic
	  4.0 %: Golgi
	  4.0 %: nuclear

>> prediction for VHB640 is exc
5' end seq. ID VHB640F
5' end seq. 
>VHB640F.Seq
AGAATATTTTAGATTAAAAAACAAAATGAGAGTTTTGTTACTTTTAGTTTTCCTCGTATT
TTGTATCATTCCAGCAGCTCTCTCATTCAGAGACACCAACTGGAGCAAATACAACAGAGC
TGGTAAAAAAGAAACTGTAGATTTCCGTATTGCACTCAATCAAAGAAATTTAGATGTCTT
AGAAAATACTTTATTGGATGTTTCTAACCCAGAATCACCAAATTATGGTAAACATTGGAC
TTCTGAACAAGTTGCTGATTTAGTTGCACCAGAACCATACGTTTCAAAGAAAGTTGCAAA
TTATTTAAAAAAGAACGGTTGTCACAATGTTGAAAATCATCGTGATTACATTAAAGCAAC
TGGTCCAGTTGATTCAGTTGAAAAGATTTTCTCAGTTGAAATGTACAATTACAAACATAA
TATTAAATCCGATCAAGCTATCATTCGTTCATCAGAAACCTATCAAGTTCCATTTGAAAT
CTCTCAATATGTTCACATTGTTTCTGGTATCTCTGAACTCCCACATCTCAAGAGTGGTCC
AAAATCACAAAGATTAAGACCANNNNNNNNNN
Length of 5' end seq. 572
3' end seq. ID VHB640Z
3' end seq. 
>VHB640Z.Seq
NNNNNNNNNNCTTCAGGTGATCAAGGTGCCCCAGGTGATGGTAATCCATCATGTACCTCA
AGAGATCAACCAATCTCAACCATTTTCCCAGGTGCCTCCCCCATGGGTTACCTCTGTTGG
TGCTACCATGTTAGCTGCCCCATCCACCAGCAATCCAAATCCAATCGATACTTCTGATGC
CCCAGTCTGTGGTATCCACGCTTGTGCTACCTCAACTCAAGAAGTTACCTGTTCATCTCC
ATCCGCTTTGATCACTACTGGTGGTGGTTTCTCCAACTACTCCCCACAACCAGCATGGCA
ATCAAAAGCTGTCAATGCTTACCTCAACTCTGGTGTTGTCTTACCAAACAAAAAATTCTT
TAACGCTTCAAACCGTGGTTTCCCAGATGTCTCTGGTTTAGGTCACAACTACTACATTGT
TGCCGCCGATCAAATGAACTTAGTCGATGGTACCTCTTGTTCATCACCAGTCATTGGTGG
TATTGTTGCCTTATTAAACAGCTATCGTTTAGATAATGGTAAATCAACCCTCGGTTTCAT
TAACCCAGTCTTATATGCTGCTTACGCCTCTAACCCAGCTGCCTTCAATGATATCACCGT
TGGTAACAATTATTGTACTGAAAGCTGTTGTGCTACCACTGGTTTCGTTGCCACCAAAGG
TTGGGATGCTGTCACTGGTTTAGGTTCACCAAATTTCAAACCATTATTATCATACATTCA
ATCTTTAAATTAAATAAATAAAATTATTTAATAATAAAAATAAAATAATAATATTATCTA
AAAA
Length of 3' end seq. 784
Connected seq. ID VHB640P
Connected seq. 
>VHB640P.Seq
AGAATATTTTAGATTAAAAAACAAAATGAGAGTTTTGTTACTTTTAGTTTTCCTCGTATT
TTGTATCATTCCAGCAGCTCTCTCATTCAGAGACACCAACTGGAGCAAATACAACAGAGC
TGGTAAAAAAGAAACTGTAGATTTCCGTATTGCACTCAATCAAAGAAATTTAGATGTCTT
AGAAAATACTTTATTGGATGTTTCTAACCCAGAATCACCAAATTATGGTAAACATTGGAC
TTCTGAACAAGTTGCTGATTTAGTTGCACCAGAACCATACGTTTCAAAGAAAGTTGCAAA
TTATTTAAAAAAGAACGGTTGTCACAATGTTGAAAATCATCGTGATTACATTAAAGCAAC
TGGTCCAGTTGATTCAGTTGAAAAGATTTTCTCAGTTGAAATGTACAATTACAAACATAA
TATTAAATCCGATCAAGCTATCATTCGTTCATCAGAAACCTATCAAGTTCCATTTGAAAT
CTCTCAATATGTTCACATTGTTTCTGGTATCTCTGAACTCCCACATCTCAAGAGTGGTCC
AAAATCACAAAGATTAAGACCA----------CTTCAGGTGATCAAGGTGCCCCAGGTGA
TGGTAATCCATCATGTACCTCAAGAGATCAACCAATCTCAACCATTTTCCCAGGTGCCTC
CCCCATGGGTTACCTCTGTTGGTGCTACCATGTTAGCTGCCCCATCCACCAGCAATCCAA
ATCCAATCGATACTTCTGATGCCCCAGTCTGTGGTATCCACGCTTGTGCTACCTCAACTC
AAGAAGTTACCTGTTCATCTCCATCCGCTTTGATCACTACTGGTGGTGGTTTCTCCAACT
ACTCCCCACAACCAGCATGGCAATCAAAAGCTGTCAATGCTTACCTCAACTCTGGTGTTG
TCTTACCAAACAAAAAATTCTTTAACGCTTCAAACCGTGGTTTCCCAGATGTCTCTGGTT
TAGGTCACAACTACTACATTGTTGCCGCCGATCAAATGAACTTAGTCGATGGTACCTCTT
GTTCATCACCAGTCATTGGTGGTATTGTTGCCTTATTAAACAGCTATCGTTTAGATAATG
GTAAATCAACCCTCGGTTTCATTAACCCAGTCTTATATGCTGCTTACGCCTCTAACCCAG
CTGCCTTCAATGATATCACCGTTGGTAACAATTATTGTACTGAAAGCTGTTGTGCTACCA
CTGGTTTCGTTGCCACCAAAGGTTGGGATGCTGTCACTGGTTTAGGTTCACCAAATTTCA
AACCATTATTATCATACATTCAATCTTTAAATTAAATAAATAAAATTATTTAATAATAAA
AATAAAATAATAATATTATCTAAAAA
Length of connected seq. 1336
Full length Seq ID -
Full length Seq. -
Length of full length seq. -