>VHB627 (VHB627Q) /CSM/VH/VHB6-B/VHB627Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTCCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCGACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCCAGXXXXXXXXXXATAACCACGAA
TGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAAT
CTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTT
GGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATC
CGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAA
ATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTCTC
TTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTAC
TCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCA
TTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTA
CGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATT
GGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAA
GGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAA
GTCGCACTTTNAGTTTTCAAANATAGTAATCAAATCTAAAAAAAA

LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRPDEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVDKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVTVSP---

---NHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCD
ENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITAPEN
AIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVF
DHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*valxvfkxsnqi*k
k

Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlkirnsvmslypq---

---ittngikmmp*tfglsvqkvtvpisssmspkvfni*mklkilslvlsngppkkvlfv
mkt*evsvsifmmspstlmpstevvvkssqllvvysmlln*lppqls*nqst**kllpqk
tllvvftlysivvevlllvknvvsvphyslskliyqy*npsvspliyvptlpvklshnvs
siiglqlvlstkirkplksl*pperekv*pqksqiltnstknskqfkshfxfsxiviksk
k

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmss**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirrqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r*gir*chcip---

---*prmg*k*chehlvfrsrr*rcqsprqchqrcsife*n*rffrwcfpmghqrrccl*
*kherypfqsl*chpph*chpqrwwsnhpncssctlcc*inclpnslrtnllsrnycprk
rywwyllctqss*rycyw*rtsyrfpiilcqssftsirilrfhr*ftfphcrssfptmcl
rslgfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnlsrtxsfqx**snlk
k

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRPDEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVDKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVTVSP---

---NHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCD
ENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITAPEN
AIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVF
DHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*valxvfkxsnqi*k
k

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ446 (VHQ446Q) /CSM/VH/VHQ4-B/VHQ446Q.Seq.d/                       1392   0.0
VHQ318 (VHQ318Q) /CSM/VH/VHQ3-A/VHQ318Q.Seq.d/                       1392   0.0
VHP769 (VHP769Q) /CSM/VH/VHP7-C/VHP769Q.Seq.d/                       1392   0.0
VHO836 (VHO836Q) /CSM/VH/VHO8-B/VHO836Q.Seq.d/                       1392   0.0
VHO414 (VHO414Q) /CSM/VH/VHO4-A/VHO414Q.Seq.d/                       1392   0.0
VHN719 (VHN719Q) /CSM/VH/VHN7-A/VHN719Q.Seq.d/                       1392   0.0
VHK606 (VHK606Q) /CSM/VH/VHK6-A/VHK606Q.Seq.d/                       1392   0.0
VHK218 (VHK218Q) /CSM/VH/VHK2-A/VHK218Q.Seq.d/                       1392   0.0
VHK172 (VHK172Q) /CSM/VH/VHK1-C/VHK172Q.Seq.d/                       1392   0.0
VHJ871 (VHJ871Q) /CSM/VH/VHJ8-C/VHJ871Q.Seq.d/                       1392   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.  1241   0.0    2
AF107291|AF107291.1 Candida parapsilosis elongation factor 2 (EFT2) gene, partial cds.    72   7e-71  13
AF107286|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds.    78   2e-70  9
Y09664|Y09664.1 C.albicans EFT2 gene.                              78   5e-69  9
AF107289|AF107289.1 Candida tropicalis elongation factor 2 (EFT2) gene, partial cds.    70   7e-66  10
AF107290|AF107290.1 Candida lusitaniae elongation factor 2 (EFT2) gene, partial cds.    64   6e-49  8
AF534908|AF534908.2 Tetrahymena thermophila elongation factor 2 gene, complete cds.    84   4e-47  6
AY144999|AY144999.1 Saccharomyces kluyveri clone Contig1706 EFT2 gene, complete cds.    78   4e-45  7
AR550596|AR550596.1 Sequence 5727 from patent US 6747137.          78   2e-44  6
EH015431|EH015431.1 USDA-FP_188032 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt081_G08 5', mRNA sequence.    78   6e-43  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   286   1e-75
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   285   3e-75
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   283   8e-75
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   283   8e-75
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   283   1e-74
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   283   1e-74
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   282   2e-74
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   281   5e-74
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   281   5e-74
AK298498_1(AK298498|pid:none) Homo sapiens cDNA FLJ56548 complet...   281   5e-74

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHB627 is cyt

>VHB627F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTCCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCGACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCCAGNNNNNNNNNN

>VHB627Z.Seq
NNNNNNNNNNATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGT
CCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAAT
GAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGT
GATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCC
ATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAA
TTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAA
AACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAA
GAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCC
TTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTC
TTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCT
TTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCAC
GAAAAACTCTAAACAATTTAAGTCGCACTTTNAGTTTTCAAANATAGTAATCAAATCTAA
AAAAAA

>VHB627P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTCCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCGACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCCAG----------ATAACCACGAA
TGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAAT
CTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTT
GGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATC
CGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAA
ATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTCTC
TTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTAC
TCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCA
TTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTA
CGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATT
GGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAA
GGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAA
GTCGCACTTTNAGTTTTCAAANATAGTAATCAAATCTAAAAAAAA