>VHB625 (VHB625Q) /CSM/VH/VHB6-B/VHB625Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCXXXXXXXXXXGCTTCACCAATCTC
TATGGAATTACAAGATCTCATTGAAGCTGGTTCTGATATCTCATCCAAAGATGATCCAAA
AGCTCGTGCTAACTATCTCGCTGATAACCACGAATGGGATAAAAATGATGCCATGAACAT
TTGGTCTTTCGGTCCAGGAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGT
TCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGA
AGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCT
CCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACT
CTATGCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAAT
TACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTAT
TGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACC
AGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTT
CCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGC
CACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCT
TGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTNAGTTTTCAAA

LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVTVS---

---ASPISMELQDLIEAGSDISSKDDPKARANYLADNHEWDKNDAMNIWSFGPGGNGANL
LVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQI
IPTARRVLYAAELTASPTLLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPL
FSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKG
LAPEIPDLDKFHEKL*ti*valxfs

Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlkirnsvmslyp---

---ASPISMELQDLIEAGSDISSKDDPKARANYLADNHEWDKNDAMNIWSFGPGGNGANL
LVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQI
IPTARRVLYAAELTASPTLLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPL
FSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKG
LAPEIPDLDKFHEKL*ti*valxfs

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r*gir*chci---

---lhqslwnykislklvlishpkmiqklvltislittngikmmp*tfglsvqevtvpis
ssmspkvfni*mklkilslvlsngppkkvlfvmkt*evsvsifmmspstlmpstevvvks
sqllvvysmlln*lppqls*nqst**kllpqktllvvftlysivvevlllvknvvsvphy
slskliyqy*npsvspliyvptlpvklshnvssiiglqlvlstkirkplksl*pperekv
*pqksqiltnstknskqfkshxsfq

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVTVS---

---ftnlygitrsh*swf*yliqr*skssc*lsr**prmg*k*chehlvfrsrr*rcqsp
rqchqrcsife*n*rffrwcfpmghqrrccl**kherypfqsl*chpph*chpqrwwsnh
pncssctlcc*inclpnslrtnllsrnycprkrywwyllctqss*rycyw*rtsyrfpii
lcqssftsirilrfhr*ftfphcrssfptmclrslgfnwccqqr*esh*srfshqkekrf
sprnprs*qiprktlnnlsrtxvfk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB625 (VHB625Q) /CSM/VH/VHB6-B/VHB625Q.Seq.d/                       2738   0.0
CFC418 (CFC418Q) /CSM/CF/CFC4-A/CFC418Q.Seq.d/                       1534   0.0
VHI722 (VHI722Q) /CSM/VH/VHI7-A/VHI722Q.Seq.d/                       1509   0.0
VHK412 (VHK412Q) /CSM/VH/VHK4-A/VHK412Q.Seq.d/                       1497   0.0
VHC551 (VHC551Q) /CSM/VH/VHC5-C/VHC551Q.Seq.d/                       1497   0.0
VHA217 (VHA217Q) /CSM/VH/VHA2-A/VHA217Q.Seq.d/                       1487   0.0
VHH525 (VHH525Q) /CSM/VH/VHH5-B/VHH525Q.Seq.d/                       1457   0.0
VHC759 (VHC759Q) /CSM/VH/VHC7-C/VHC759Q.Seq.d/                       1453   0.0
VHP324 (VHP324Q) /CSM/VH/VHP3-A/VHP324Q.Seq.d/                       1451   0.0
VHH853 (VHH853Q) /CSM/VH/VHH8-C/VHH853Q.Seq.d/                       1451   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.  1423   0.0    2
AF107291|AF107291.1 Candida parapsilosis elongation factor 2 (EFT2) gene, partial cds.    72   2e-68  13
AF107286|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds.    78   6e-68  9
Y09664|Y09664.1 C.albicans EFT2 gene.                              78   1e-66  9
AF107289|AF107289.1 Candida tropicalis elongation factor 2 (EFT2) gene, partial cds.    70   2e-63  10
AF534908|AF534908.2 Tetrahymena thermophila elongation factor 2 gene, complete cds.    84   5e-47  6
AF107290|AF107290.1 Candida lusitaniae elongation factor 2 (EFT2) gene, partial cds.    64   2e-46  8
AY144999|AY144999.1 Saccharomyces kluyveri clone Contig1706 EFT2 gene, complete cds.    78   4e-44  8
EH015431|EH015431.1 USDA-FP_188032 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt081_G08 5', mRNA sequence.    78   8e-43  4
EH012247|EH012247.1 USDA-FP_185167 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt044_H07 5', mRNA sequence.    78   1e-42  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   307   7e-82
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   306   2e-81
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   302   2e-80
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   302   2e-80
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   302   2e-80
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   302   2e-80
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   301   4e-80
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   299   2e-79
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   299   2e-79
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   299   2e-79

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHB625 is cyt

>VHB625F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCNNNNNNNNNN

>VHB625Z.Seq
NNNNNNNNNNGCTTCACCAATCTCTATGGAATTACAAGATCTCATTGAAGCTGGTTCTGA
TATCTCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGCTGATAACCACGAATG
GGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGGAGGTAACGGTGCCAATCT
CCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGG
TGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCG
TTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAAT
CATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTCTCTT
AGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTC
TGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATT
ATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACG
TTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGG
TGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGG
TTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGT
CGCACTTNAGTTTTCAAA

>VHB625P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCC----------GCTTCACCAATCTC
TATGGAATTACAAGATCTCATTGAAGCTGGTTCTGATATCTCATCCAAAGATGATCCAAA
AGCTCGTGCTAACTATCTCGCTGATAACCACGAATGGGATAAAAATGATGCCATGAACAT
TTGGTCTTTCGGTCCAGGAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGT
TCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGA
AGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCT
CCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACT
CTATGCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAAT
TACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTAT
TGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACC
AGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTT
CCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGC
CACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCT
TGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTNAGTTTTCAAA