>VHB614 (VHB614Q) /CSM/VH/VHB6-A/VHB614Q.Seq.d/
GAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTCAAAATGAAGA
ATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGCAACAATTAAC
TAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAGAAGAAGCATT
AGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAATTTATAATAA
ACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAATTTTCAATAA
AGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCAGA
AAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAATCATCAGAAGG
TGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTATCAGCATTTGT
AAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGATCTATCAATC
ACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTGTCTATTATTC
AATGATTGATATATTTTCAGTATTAATAAATTTAGATAGTATAATTACACAAAATACTGC
TTXXXXXXXXXXTTAGAGAGAATAAAGAACAATTGAATAATATGTACCNTTCAGAGTTGG
TGACAGAGTTGGAAAGAGACATTAGACAATTGGGTGTCTCCGAGACTGGTTTAACCTTTT
TAGATCATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGGTTATATTAGATTGG
TCAGATCCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGTACCAGACTTGAAAA
AGATTCCAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAG
CTAGTACCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTG
AATACTTTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAAC
ATTTAAAGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGA
TCAATAGTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTAT
TCACTGATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATA
AAGATTTCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAACTGATCAAA
AGAGAATGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAAAATCATTTAGCAA
TTAAAAAGATTCAAAATTTCCAACAAGAATAGAATTATTTAAATATTCTTTCNCGGTTCA
AG

nnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIEEAL
DETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILKETAE
SKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASIYQS
QKNIHTQFFKNVHLQAVYYSMIDIFSVLINLDSIITQNTA---

---RENKEQLNNMYXSELVTELERDIRQLGVSETGLTFLDHFRLLITHIGNAMGYIRLVR
SGGLHYCSNAIKFVPDLKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNLSEGTEY
FKMLVNVFATEFRNIANQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGAEALLFT
DDGFAIGLAYILKLLDQNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKEDQNHLAIK
KIQNFQQE*nylnilsrfk

Frame A:
eqpnlenghilfg**wsk*riyi*ci*rwfi*igwratin*t*sics*lcttnledrrsi
r*nik*smgykyrssfnl**ts*tirfn*ig*n*s*nfq*snisilfnl*sn*nfkrnsr
k*ilftinsiwrdyr*iirr*ctn*sw*iitiyd*fisick*mlfin*kyy*sicidlsi
tkeytytil*ecsftscllfnd*yifsinkfr*ynytkyc---

---leriknn*iictxqsw*qswketldnwvsprlv*pf*iildf*slilvmlwvildws
dpvvytivvmlsnsyqt*krfqnski*pqkmpshlkqfklvpiwiqsfiifqiiyqrvln
tlrcw*tfslqsfeislini*risissylpwpqitsii*sivkinslrnqkqsvlklyys
lmmalqlvwlis*ny*ikikisilfigsivfn*nvklikrecwlkqmqkvskrikii*ql
krfkisnknrii*iffxgs

Frame B:
nnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIEEAL
DETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILKETAE
SKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASIYQS
QKNIHTQFFKNVHLQAVYYSMIDIFSVLINLDSIITQNTA---

---*re*rtie*yvpfrvgdrvgkrh*tigclrdwfnlfrsf*tfnhsyw*cygly*igq
irwftll**cyqirtrlekdskiprfnlkrcplt*nnss*yqfgfnhs*sfk*firgy*i
l*dvgkrfryrvskyr*stfkeflyhrtclglklhrsydq**r*tl*eiksnrc*sfiih
**wlcnwfglylkiirsk*rfrfsslvrsysiem*n*skenvg*skckryqrgsksfsn*
kdskfptrielfkysfxvq

Frame C:
ttqfrkwphpirlimvkmknihlmhlkmvhlnwlesnn*lnlinlqlimhnkfgr*kkh*
mkh*mkygi*i*iqfqfiinlmnn*i*lnwlkliikfsik*y*yslqfviklef*kkqqk
vnfihh*qylerlqvnhqkvmyklklvnyyhl*liyqhl*idvih*leillvnlhrsinh
kriyihnslrmfiyklsiiq*liyfqy**i*iv*lhkill---

---RENKEQLNNMYXSELVTELERDIRQLGVSETGLTFLDHFRLLITHIGNAMGYIRLVR
SGGLHYCSNAIKFVPDLKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNLSEGTEY
FKMLVNVFATEFRNIANQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGAEALLFT
DDGFAIGLAYILKLLDQNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKEDQNHLAIK
KIQNFQQE*nylnilsrfk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB614 (VHB614Q) /CSM/VH/VHB6-A/VHB614Q.Seq.d/                       2787   0.0
SHK609 (SHK609Q) /CSM/SH/SHK6-A/SHK609Q.Seq.d/                       2757   0.0
VHJ241 (VHJ241Q) /CSM/VH/VHJ2-B/VHJ241Q.Seq.d/                       2738   0.0
VHA684 (VHA684Q) /CSM/VH/VHA6-D/VHA684Q.Seq.d/                       1515   0.0
CHG805 (CHG805Q) /CSM/CH/CHG8-A/CHG805Q.Seq.d/                       1515   0.0
VHI518 (VHI518Q) /CSM/VH/VHI5-A/VHI518Q.Seq.d/                       1509   0.0
VHK424 (VHK424Q) /CSM/VH/VHK4-A/VHK424Q.Seq.d/                       1507   0.0
AHN345 (AHN345Q) /CSM/AH/AHN3-B/AHN345Q.Seq.d/                       1503   0.0
VHK538 (VHK538Q) /CSM/VH/VHK5-B/VHK538Q.Seq.d/                       1501   0.0
VHB821 (VHB821Q) /CSM/VH/VHB8-A/VHB821Q.Seq.d/                       1501   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BU746157|BU746157.1 CH3#002_C07T7 Canine heart normalized cDNA Library in pBluescriptCanis familiaris cDNA clone CH3#002_C07 5', mRNA sequence.    38   0.001  3
CP000263|CP000263.1 Buchnera aphidicola str. Cc (Cinara cedri), complete genome.    36   0.022  15
BY464171|BY464171.1 Mus musculus 15 days pregnant adult female amnion cDNA, RIKEN full-length enriched library, clone:K630150M24, 3' end partial sequence.    42   0.036  2
AK113047|AK113047.1 Ciona intestinalis cDNA, clone:ciad002a21, full insert sequence.    48   0.044  2
AF063866|AF063866.1 Melanoplus sanguinipes entomopoxvirus, complete genome.    32   0.050  13
CP000123|CP000123.1 Mycoplasma capricolum subsp. capricolum ATCC 27343, complete genome.    36   0.077  21
CP000395|CP000395.1 Borrelia afzelii PKo, complete genome.         32   0.16   18
BB822673|BB822673.1 Mus musculus mammary gland RCB-0526 Jyg-MC(A) cDNA, RIKEN full-length enriched library, clone:G830022O04, 3' end partial sequence.    32   0.16   4
CR103667|CR103667.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP142o16.    50   0.18   1
CR044130|CR044130.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP186g09.    50   0.18   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54R74) RecName: Full=UPF0681 protein;                               486   e-135
BC040936_1(BC040936|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   237   7e-61
AB028956_1(AB028956|pid:none) Homo sapiens mRNA for KIAA1033 pro...   237   9e-61
BC143373_1(BC143373|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   237   9e-61
BC020314_1(BC020314|pid:none) Mus musculus RIKEN cDNA A230046K03...   235   3e-60
(Q3UMB9) RecName: Full=UPF0681 protein KIAA1033;                      235   3e-60
AK161247_1(AK161247|pid:none) Mus musculus adult male testis cDN...   235   3e-60
AK145000_1(AK145000|pid:none) Mus musculus mammary gland RCB-052...   235   3e-60
BC110850_1(BC110850|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   182   3e-44
AX882617_1(AX882617|pid:none) Sequence 17522 from Patent EP10746...   177   8e-43

 psg: 0.79  gvh: 0.25  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VHB614 is nuc

>VHB614F.Seq
GAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTCAAAATGAAGA
ATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGCAACAATTAAC
TAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAGAAGAAGCATT
AGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAATTTATAATAA
ACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAATTTTCAATAA
AGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCAGA
AAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAATCATCAGAAGG
TGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTATCAGCATTTGT
AAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGATCTATCAATC
ACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTGTCTATTATTC
AATGATTGATATATTTTCAGTATTAATAAATTTAGATAGTATAATTACACAAAATACTGC
TTNNNNNNNNNN

>VHB614Z.Seq
NNNNNNNNNNTTAGAGAGAATAAAGAACAATTGAATAATATGTACCNTTCAGAGTTGGTG
ACAGAGTTGGAAAGAGACATTAGACAATTGGGTGTCTCCGAGACTGGTTTAACCTTTTTA
GATCATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGGTTATATTAGATTGGTC
AGATCCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAAG
ATTCCAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCT
AGTACCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAA
TACTTTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACAT
TTAAAGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGATC
AATAGTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTC
ACTGATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAA
GATTTCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAACTGATCAAAAG
AGAATGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAAAATCATTTAGCAATT
AAAAAGATTCAAAATTTCCAACAAGAATAGAATTATTTAAATATTCTTTCNCGGTTCAAG

>VHB614P.Seq
GAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTCAAAATGAAGA
ATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGCAACAATTAAC
TAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAGAAGAAGCATT
AGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAATTTATAATAA
ACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAATTTTCAATAA
AGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCAGA
AAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAATCATCAGAAGG
TGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTATCAGCATTTGT
AAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGATCTATCAATC
ACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTGTCTATTATTC
AATGATTGATATATTTTCAGTATTAATAAATTTAGATAGTATAATTACACAAAATACTGC
TT----------TTAGAGAGAATAAAGAACAATTGAATAATATGTACCNTTCAGAGTTGG
TGACAGAGTTGGAAAGAGACATTAGACAATTGGGTGTCTCCGAGACTGGTTTAACCTTTT
TAGATCATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGGTTATATTAGATTGG
TCAGATCCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGTACCAGACTTGAAAA
AGATTCCAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAG
CTAGTACCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTG
AATACTTTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAAC
ATTTAAAGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGA
TCAATAGTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTAT
TCACTGATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATA
AAGATTTCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAACTGATCAAA
AGAGAATGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAAAATCATTTAGCAA
TTAAAAAGATTCAAAATTTCCAACAAGAATAGAATTATTTAAATATTCTTTCNCGGTTCA
AG