>VHB605 (VHB605Q) /CSM/VH/VHB6-A/VHB605Q.Seq.d/
AAAATTATTATTTATAAATAATCTTTATCTTTAACTCTATATATATAAAAAATAATGGCC
GATTTAAACTCTGTTATTAGAATTAAACCATTTCATTTCATTCACGTATTAGATAATAAT
ACAAATGTTACTAGAGTTGAAGTTGGACCACAAACTTTTACAAGACAAGATCATGAAAAG
TTAGTAAGTGGCCCAGAACCAATGATTATGATCCCACAACGTAACTATTGTACAATTTCA
AATCCAGTAGTTCGTAATGAAAATGGTTCATTAGTATTGGATGAATATGGTCAAGTTAAA
TTAAGACATGGTGATGAAGAGATTCGTTTCTCACAAGAACCATTCCCATTATATCCAGGT
GAAAAAATTTCAGGTAAAGTTACCGCTCTCCAAGTTGTTGCACCATTAAAGGCTTTACGT
CTTCGTGCTCTCCGTGATTTCACTGAAGCTAAAGTCACTCATGTTGCTGGTGATGAATGG
TTATTTGAAGGTCCAGCAACCTATCTCCCACGTATTGATGTTAGAATTGAAGAAGAAATC
AAAGCAACCATCATTGGACCAAATCAAGCACTCAAATTACGTGCCAACAAAGCATGTTCC
GATCGTTCAGGTGTTGCAAGAAAAGCTGGTGAAGAGTGGTTAGTTCGTCAACATGGXXXX
XXXXXXTTCAATTCAAACAACCTTTGTCATCACCAACATTGATATTCAATCTGTTGAACC
AGTCGATCAACGTACTCGTGATTCACTCCAGAAATCTGTCCAATTGGCCATTGAAATCAC
CACCAAATCACAAGAAGCTGCTGCTCGTCATGAAGCTGAACGTCTTGAACAAGGTGCTCG
TGGTAGACTCGAACGTCAAAAGATTCACGATGAAGCTCAAGCAGAGTTAGCTCGTAAAGA
TTTATTACAACTCCAAGCTCAATCAGCTGCAGTCGAGTCAACTGGTCAAGCTACCGCTGA
AGCTCAAGCTACTGCTGAAGCTGCTAACATTGCTGCTGAAGCCAATGTTAAACAAGCTGA
ATTAAAAGCTCAAGCCACCAAGATTCGTTCAGAATCTGAAATTTCTCTCTTAAAAGCCAA
GAGAGAAAATGAACTCTCATACCAAAAATCAATTGATGAACTCGAACTCACCAAACAATC
TGATCTCGCTGAAATTGAAGCCTCCAAATTCAAAGCAATCGTGGAATCCATTGGTAGAGA
TACCCTCAAATCAATTGCTTGTGCTGGTAATGAAATGCAAGCTAAATTATTACAAGGTTT
GGGTCTTAAATCATTTATGATCACCGACGGTAAATCACCACTCAACCTTTTCGATACCGC
CAACGGTATCATTGGTAATAACAATCAAATGGTAAACATGTCAAATGCTGCCAAACAATN
CGGTAGAAGAAATTAAAAAAAAATAAT

kiiiyk*slsltlyi*KIMADLNSVIRIKPFHFIHVLDNNTNVTRVEVGPQTFTRQDHEK
LVSGPEPMIMIPQRNYCTISNPVVRNENGSLVLDEYGQVKLRHGDEEIRFSQEPFPLYPG
EKISGKVTALQVVAPLKALRLRALRDFTEAKVTHVAGDEWLFEGPATYLPRIDVRIEEEI
KATIIGPNQALKLRANKACSDRSGVARKAGEEWLVRQH---

---SIQTTFVITNIDIQSVEPVDQRTRDSLQKSVQLAIEITTKSQEAAARHEAERLEQGA
RGRLERQKIHDEAQAELARKDLLQLQAQSAAVESTGQATAEAQATAEAANIAAEANVKQA
ELKAQATKIRSESEISLLKAKRENELSYQKSIDELELTKQSDLAEIEASKFKAIVESIGR
DTLKSIACAGNEMQAKLLQGLGLKSFMITDGKSPLNLFDTANGIIGNNNQMVNMSNAAKQ
XGRRN*kki

Frame A:
kiiiyk*slsltlyi*KIMADLNSVIRIKPFHFIHVLDNNTNVTRVEVGPQTFTRQDHEK
LVSGPEPMIMIPQRNYCTISNPVVRNENGSLVLDEYGQVKLRHGDEEIRFSQEPFPLYPG
EKISGKVTALQVVAPLKALRLRALRDFTEAKVTHVAGDEWLFEGPATYLPRIDVRIEEEI
KATIIGPNQALKLRANKACSDRSGVARKAGEEWLVRQH---

---fnsnnlchhqh*ysic*tsrstys*ftpeicpigh*nhhqitrsccss*s*ts*trc
sw*trtskdsr*sssrvss*rfittpssiscsrvnwssyr*sssyc*sc*hcc*sqc*ts
*ikssshqdsfri*nfslksqerk*tlipkin**trthqti*sr*n*slqiqsnrgihw*
rypqinclcw**nas*iitrfgs*iiydhrr*ittqpfryrqryhw**qsngkhvkccqt
xr*kklkknn

Frame B:
kllfinnlyl*lyiykk*wpi*tlllelnhfisfty*iiiqmllelkldhkllqdkimks
**vaqnq*l*shnvtivqfqiq*fvmkmvh*ywmnmvkln*dmvmkrfvshknhshyiqv
kkfqvklplskllhh*rlyvfvlsvislklkslmllvmngylkvqqpishvlmlelkkks
kqpsldqikhsnyvptkhvpivqvlqeklvksg*fvnm---

---SIQTTFVITNIDIQSVEPVDQRTRDSLQKSVQLAIEITTKSQEAAARHEAERLEQGA
RGRLERQKIHDEAQAELARKDLLQLQAQSAAVESTGQATAEAQATAEAANIAAEANVKQA
ELKAQATKIRSESEISLLKAKRENELSYQKSIDELELTKQSDLAEIEASKFKAIVESIGR
DTLKSIACAGNEMQAKLLQGLGLKSFMITDGKSPLNLFDTANGIIGNNNQMVNMSNAAKQ
XGRRN*kki

Frame C:
nyyl*iififnsiyiknngrfklcy*n*tisfhsrir**ykcy*s*swttnfyktrs*kv
skwprtndydptt*llynfkssss**kwfisig*iwss*iktw**rdsfltrtipiisr*
knfr*syrspscctikgftsscsp*fh*s*shsccw**mvi*rssnlspty*c*n*rrnq
snhhwtksstqitcqqsmfrsfrcckksw*rvvssstw---

---qfkqplssptlifnllnqsinvlvihsrnlsnwplksppnhkklllvmklnvlnkvl
vvdsnvkrftmklkqs*lvkiyynsklnqlqssqlvklplklklllklltlllkpmlnkl
n*klkpprfvqnlkfls*kprekmnshtknqlmnsnspnnlislklkppnskqswnplve
ipsnqllvlvmkcklnyykvwvlnhl*sptvnhhstfsipptvslvitikw*tcqmlpnn
xveeikkk*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB605 (VHB605Q) /CSM/VH/VHB6-A/VHB605Q.Seq.d/                       2698   0.0
VHQ344 (VHQ344Q) /CSM/VH/VHQ3-B/VHQ344Q.Seq.d/                       1403   0.0
CHF884 (CHF884Q) /CSM/CH/CHF8-D/CHF884Q.Seq.d/                       1403   0.0
AHE895 (AHE895Q) /CSM/AH/AHE8-D/AHE895Q.Seq.d/                       1398   0.0
VHP312 (VHP312Q) /CSM/VH/VHP3-A/VHP312Q.Seq.d/                       1388   0.0
AHB516 (AHB516Q) /CSM/AH/AHB5-A/AHB516Q.Seq.d/                       1386   0.0
VHA386 (VHA386Q) /CSM/VH/VHA3-D/VHA386Q.Seq.d/                       1350   0.0
VHH682 (VHH682Q) /CSM/VH/VHH6-D/VHH682Q.Seq.d/                       1344   0.0
AHI488 (AHI488Q) /CSM/AH/AHI4-D/AHI488Q.Seq.d/                       1338   0.0
AHE859 (AHE859Q) /CSM/AH/AHE8-C/AHE859Q.Seq.d/                       1316   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L08646|L08646.1 Dictyostelium discoideum major vault protein alpha (MVPalpha) mRNA, complete cds.  1372   0.0    2
Z37109|Z37109.1 D.discoideum mRNA for major vault protein B.      188   e-140  8
EG016576|EG016576.1 EST01313_0906 Sporophyte cDNA Library Porphyra haitanensis cDNA 5', mRNA sequence.   119   5e-26  2
EC132986|EC132986.1 HVE00006496 Hartmannella vermiformis Regular Large Hartmannella vermiformis cDNA, mRNA sequence.    66   2e-23  3
AT009029|AT009029.1 Clonorchis sinensis mRNA, clone: CSAD-25-B10, 5' EST.   117   9e-22  1
CT836653|CT836653.1 Oryza sativa (indica cultivar-group) cDNA clone:OSIGCRA216N21, full  insert sequence.    54   7e-13  5
CK740021|CK740021.1 USDA-FP_6515 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE64E02 5', mRNA sequence.    52   2e-11  2
CX835130|AF255664_1 major vault protein [Ictalurus punctatus], mRNA sequence.    60   1e-10  2
DN137794| MVP100 [Torpedo marmorata], mRNA sequence.    52   2e-08  2
EC761254|EC761254.1 PSE00001282 rw_mgpallid Polysphondylium pallidum cDNA, mRNA sequence.    56   1e-06  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54659) RecName: Full=Major vault protein beta;          Short=...   246   1e-63
AJ720187_1(AJ720187|pid:none) Gallus gallus partial mRNA for hyp...   219   2e-55
AK167899_1(AK167899|pid:none) Mus musculus TIB-55 BB88 cDNA, RIK...   217   7e-55
AK152534_1(AK152534|pid:none) Mus musculus bone marrow macrophag...   217   7e-55
(Q62667) RecName: Full=Major vault protein;          Short=MVP; ...   217   7e-55
(Q9EQK5) RecName: Full=Major vault protein;          Short=MVP; ...   217   7e-55
AK088046_1(AK088046|pid:none) Mus musculus 2 days neonate thymus...   217   9e-55
BC049344_1(BC049344|pid:none) Danio rerio major vault protein, m...   216   2e-54
(Q90405) RecName: Full=Major vault protein; AltName: Full=MVP100...   215   3e-54
S57723(S57723)lrp protein - human                                     215   4e-54

 psg: 0.74  gvh: 0.30  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: plasma membrane
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VHB605 is cyt

>VHB605F.Seq
AAAATTATTATTTATAAATAATCTTTATCTTTAACTCTATATATATAAAAAATAATGGCC
GATTTAAACTCTGTTATTAGAATTAAACCATTTCATTTCATTCACGTATTAGATAATAAT
ACAAATGTTACTAGAGTTGAAGTTGGACCACAAACTTTTACAAGACAAGATCATGAAAAG
TTAGTAAGTGGCCCAGAACCAATGATTATGATCCCACAACGTAACTATTGTACAATTTCA
AATCCAGTAGTTCGTAATGAAAATGGTTCATTAGTATTGGATGAATATGGTCAAGTTAAA
TTAAGACATGGTGATGAAGAGATTCGTTTCTCACAAGAACCATTCCCATTATATCCAGGT
GAAAAAATTTCAGGTAAAGTTACCGCTCTCCAAGTTGTTGCACCATTAAAGGCTTTACGT
CTTCGTGCTCTCCGTGATTTCACTGAAGCTAAAGTCACTCATGTTGCTGGTGATGAATGG
TTATTTGAAGGTCCAGCAACCTATCTCCCACGTATTGATGTTAGAATTGAAGAAGAAATC
AAAGCAACCATCATTGGACCAAATCAAGCACTCAAATTACGTGCCAACAAAGCATGTTCC
GATCGTTCAGGTGTTGCAAGAAAAGCTGGTGAAGAGTGGTTAGTTCGTCAACATGGNNNN
NNNNNN

>VHB605Z.Seq
NNNNNNNNNNTTCAATTCAAACAACCTTTGTCATCACCAACATTGATATTCAATCTGTTG
AACCAGTCGATCAACGTACTCGTGATTCACTCCAGAAATCTGTCCAATTGGCCATTGAAA
TCACCACCAAATCACAAGAAGCTGCTGCTCGTCATGAAGCTGAACGTCTTGAACAAGGTG
CTCGTGGTAGACTCGAACGTCAAAAGATTCACGATGAAGCTCAAGCAGAGTTAGCTCGTA
AAGATTTATTACAACTCCAAGCTCAATCAGCTGCAGTCGAGTCAACTGGTCAAGCTACCG
CTGAAGCTCAAGCTACTGCTGAAGCTGCTAACATTGCTGCTGAAGCCAATGTTAAACAAG
CTGAATTAAAAGCTCAAGCCACCAAGATTCGTTCAGAATCTGAAATTTCTCTCTTAAAAG
CCAAGAGAGAAAATGAACTCTCATACCAAAAATCAATTGATGAACTCGAACTCACCAAAC
AATCTGATCTCGCTGAAATTGAAGCCTCCAAATTCAAAGCAATCGTGGAATCCATTGGTA
GAGATACCCTCAAATCAATTGCTTGTGCTGGTAATGAAATGCAAGCTAAATTATTACAAG
GTTTGGGTCTTAAATCATTTATGATCACCGACGGTAAATCACCACTCAACCTTTTCGATA
CCGCCAACGGTATCATTGGTAATAACAATCAAATGGTAAACATGTCAAATGCTGCCAAAC
AATNCGGTAGAAGAAATTAAAAAAAAATAAT

>VHB605P.Seq
AAAATTATTATTTATAAATAATCTTTATCTTTAACTCTATATATATAAAAAATAATGGCC
GATTTAAACTCTGTTATTAGAATTAAACCATTTCATTTCATTCACGTATTAGATAATAAT
ACAAATGTTACTAGAGTTGAAGTTGGACCACAAACTTTTACAAGACAAGATCATGAAAAG
TTAGTAAGTGGCCCAGAACCAATGATTATGATCCCACAACGTAACTATTGTACAATTTCA
AATCCAGTAGTTCGTAATGAAAATGGTTCATTAGTATTGGATGAATATGGTCAAGTTAAA
TTAAGACATGGTGATGAAGAGATTCGTTTCTCACAAGAACCATTCCCATTATATCCAGGT
GAAAAAATTTCAGGTAAAGTTACCGCTCTCCAAGTTGTTGCACCATTAAAGGCTTTACGT
CTTCGTGCTCTCCGTGATTTCACTGAAGCTAAAGTCACTCATGTTGCTGGTGATGAATGG
TTATTTGAAGGTCCAGCAACCTATCTCCCACGTATTGATGTTAGAATTGAAGAAGAAATC
AAAGCAACCATCATTGGACCAAATCAAGCACTCAAATTACGTGCCAACAAAGCATGTTCC
GATCGTTCAGGTGTTGCAAGAAAAGCTGGTGAAGAGTGGTTAGTTCGTCAACATGG----
------TTCAATTCAAACAACCTTTGTCATCACCAACATTGATATTCAATCTGTTGAACC
AGTCGATCAACGTACTCGTGATTCACTCCAGAAATCTGTCCAATTGGCCATTGAAATCAC
CACCAAATCACAAGAAGCTGCTGCTCGTCATGAAGCTGAACGTCTTGAACAAGGTGCTCG
TGGTAGACTCGAACGTCAAAAGATTCACGATGAAGCTCAAGCAGAGTTAGCTCGTAAAGA
TTTATTACAACTCCAAGCTCAATCAGCTGCAGTCGAGTCAACTGGTCAAGCTACCGCTGA
AGCTCAAGCTACTGCTGAAGCTGCTAACATTGCTGCTGAAGCCAATGTTAAACAAGCTGA
ATTAAAAGCTCAAGCCACCAAGATTCGTTCAGAATCTGAAATTTCTCTCTTAAAAGCCAA
GAGAGAAAATGAACTCTCATACCAAAAATCAATTGATGAACTCGAACTCACCAAACAATC
TGATCTCGCTGAAATTGAAGCCTCCAAATTCAAAGCAATCGTGGAATCCATTGGTAGAGA
TACCCTCAAATCAATTGCTTGTGCTGGTAATGAAATGCAAGCTAAATTATTACAAGGTTT
GGGTCTTAAATCATTTATGATCACCGACGGTAAATCACCACTCAACCTTTTCGATACCGC
CAACGGTATCATTGGTAATAACAATCAAATGGTAAACATGTCAAATGCTGCCAAACAATN
CGGTAGAAGAAATTAAAAAAAAATAAT