>VHB595 (VHB595Q) /CSM/VH/VHB5-D/VHB595Q.Seq.d/
XXXXXXXXXXGTCAATTCATTGGCTCATTATTTAGGTGACTCTCCATACGATGATGAACA
TACTCCCAAAGGATTCAGTTGTGACTGCTATATTAACATTTGGTGAAGGTTATCATAACT
TCCATCATGAATTCCCAAATGACTATAGAAACGCTTATAAATTCTATCAATATGATCCAA
CTAAATGGTTGATCTCTGCAATGTATTATTTAGGCTTGGCTTATGATTTGAAAACTTTNT
CCAAAAATGAAATTGAAAAAGGTATGATTCAAATGCACCAAAAACATTTAGACGCAAAGA
AACAATCAATNTATTGGGGTATCAATAAAGAAGATTTACCATCAATGACAATCGATGAAT
TTAATAATTTGGTTGAAAAAGAAAATAAAAAGTTAATGATAATCAACAAAACCGTATTAG
ATGTCGAATCTTTTGTTAATGATCACCCAGGTGGTTTAGCTTACATTAAAATGGGTATTG
GTAAAGATGCAACTTCAATGTTTACTGGTGAAGTTTATGCTCATTCAAATGCTGCTAAAA
ATTTATTATGTCAATTTTCAATTGCTAAAATTGTTGATAATCATGATAAAAAACAACAAT
AATCAAAAAAACAAAC

---sihwlii*VTLHTMMNILPKDSVVTAILTFGEGYHNFHHEFPNDYRNAYKFYQYDPT
KWLISAMYYLGLAYDLKTXSKNEIEKGMIQMHQKHLDAKKQSXYWGINKEDLPSMTIDEF
NNLVEKENKKLMIINKTVLDVESFVNDHPGGLAYIKMGIGKDATSMFTGEVYAHSNAAKN
LLCQFSIAKIVDNHDKKQQ*skkq

Frame A:
---sihwlii*VTLHTMMNILPKDSVVTAILTFGEGYHNFHHEFPNDYRNAYKFYQYDPT
KWLISAMYYLGLAYDLKTXSKNEIEKGMIQMHQKHLDAKKQSXYWGINKEDLPSMTIDEF
NNLVEKENKKLMIINKTVLDVESFVNDHPGGLAYIKMGIGKDATSMFTGEVYAHSNAAKN
LLCQFSIAKIVDNHDKKQQ*skkq


Frame B:
---qfigslfr*lsir**tysqriql*lly*hlvkviitsimnsqmtietlinsinmiql
ng*slqcii*awlmi*klxpkmklkkv*fkctkni*tqrnnqxigvsikkiyhq*qsmnl
iiwlkkkiks***stkpy*msnlllmitqvv*ltlkwvlvkmqlqcllvkfmliqmllki
yyvnfqllklliimiknnnnqknk


Frame C:
---nslahylgdspyddehtpkgfscdcyiniw*rls*lps*ipk*l*krl*ilsi*sn*
mvdlcnvlfrlgl*fenfxqk*n*krydsnapktfrrketinxlgyq*rrftindnr*i*
*fg*krk*kvndnqqnrircrifc**sprwfslh*ngyw*rcnfnvyw*slcsfkcc*kf
imsifnc*nc**s**kttiikktn

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB595 (VHB595Q) /CSM/VH/VHB5-D/VHB595Q.Seq.d/                        712   0.0
VSA271 (VSA271Q) /CSM/VS/VSA2-C/VSA271Q.Seq.d/                        696   0.0
VHQ458 (VHQ458Q) /CSM/VH/VHQ4-C/VHQ458Q.Seq.d/                        696   0.0
VHP465 (VHP465Q) /CSM/VH/VHP4-C/VHP465Q.Seq.d/                        696   0.0
VHN236 (VHN236Q) /CSM/VH/VHN2-B/VHN236Q.Seq.d/                        696   0.0
VHK171 (VHK171Q) /CSM/VH/VHK1-C/VHK171Q.Seq.d/                        696   0.0
VHH185 (VHH185Q) /CSM/VH/VHH1-D/VHH185Q.Seq.d/                        696   0.0
VHE438 (VHE438Q) /CSM/VH/VHE4-B/VHE438Q.Seq.d/                        696   0.0
VHE192 (VHE192Q) /CSM/VH/VHE1-D/VHE192Q.Seq.d/                        696   0.0
VHD593 (VHD593Q) /CSM/VH/VHD5-D/VHD593Q.Seq.d/                        696   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.   603   0.0    3
AL033501|AL033501.1 C.albicans cosmid Ca41C10.                     72   4e-12  2
AL396412|AL396412.1 T3 end of clone AR0AA030B05 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    54   2e-08  2
DY893926|DY893926.1 CeleSEQ13391 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ13391, mRNA sequence.    48   3e-08  4
BM634131|BM634131.1 17000668813253 A.Gam.ad.cDNA1 Anopheles gambiae cDNA clone 19600448419669 5', mRNA sequence.    54   1e-06  2
DY885896|DY885896.1 CeleSEQ991 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ991, mRNA sequence.    48   5e-06  2
DY886077|DY886077.1 CeleSEQ1581 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ1581, mRNA sequence.    48   5e-06  2
DY895351|DY895351.1 CeleSEQ15152 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ15152, mRNA sequence.    48   5e-06  2
CR382138|CR382138.1 Debaryomyces hansenii chromosome F of strain CBS767 of Debaryomyces hansenii.    46   2e-05  2
AL392980|AL392980.1 T3 end of clone AR0AA008A11 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    44   2e-05  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116984_117(AC116984|pid:none) Dictyostelium discoideum chromos...   366   e-108
AB196774_1(AB196774|pid:none) Mortierella alpina gene for omega9...   184   7e-51
AY576801_1(AY576801|pid:none) Hortaea werneckii putative delta-9...   187   2e-50
DQ901954_1(DQ901954|pid:none) Aureobasidium pullulans putative d...   184   7e-50
AP007151_392(AP007151|pid:none) Aspergillus oryzae RIB40 genomic...   184   1e-49
FM992688_770(FM992688|pid:none) Candida dubliniensis CD36 chromo...   186   2e-49
AL033501_2(AL033501|pid:none) C.albicans cosmid Ca41C10.  &T1822...   186   2e-49
CR382123_238(CR382123|pid:none) Kluyveromyces lactis strain NRRL...   180   3e-49
CR382138_167(CR382138|pid:none) Debaryomyces hansenii strain CBS...   184   4e-49
AM920435_140(AM920435|pid:none) Penicillium chrysogenum Wisconsi...   182   4e-49

 psg: 0.85  gvh: 0.35  alm: 0.43  top: 0.37  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: vacuolar

>> prediction for VHB595 is cyt

>VHB595Z.Seq
NNNNNNNNNNGTCAATTCATTGGCTCATTATTTAGGTGACTCTCCATACGATGATGAACA
TACTCCCAAAGGATTCAGTTGTGACTGCTATATTAACATTTGGTGAAGGTTATCATAACT
TCCATCATGAATTCCCAAATGACTATAGAAACGCTTATAAATTCTATCAATATGATCCAA
CTAAATGGTTGATCTCTGCAATGTATTATTTAGGCTTGGCTTATGATTTGAAAACTTTNT
CCAAAAATGAAATTGAAAAAGGTATGATTCAAATGCACCAAAAACATTTAGACGCAAAGA
AACAATCAATNTATTGGGGTATCAATAAAGAAGATTTACCATCAATGACAATCGATGAAT
TTAATAATTTGGTTGAAAAAGAAAATAAAAAGTTAATGATAATCAACAAAACCGTATTAG
ATGTCGAATCTTTTGTTAATGATCACCCAGGTGGTTTAGCTTACATTAAAATGGGTATTG
GTAAAGATGCAACTTCAATGTTTACTGGTGAAGTTTATGCTCATTCAAATGCTGCTAAAA
ATTTATTATGTCAATTTTCAATTGCTAAAATTGTTGATAATCATGATAAAAAACAACAAT
AATCAAAAAAACAAAC