>VHB558 (VHB558Q) /CSM/VH/VHB5-C/VHB558Q.Seq.d/
AGGTCCTATATTCTAATTCTAATTAATTTTATTTGTTTTTTTTTGTAGAACATAAAAAAA
TGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATCAGCTTATGTCCC
TTATTATTAATACTTTTTATTCAAATAAAGAAGTATTTTTAAGAGAATTGATTTCAAATG
CATCTGATGCCTTAGATAAAATTCGTTACCAATCATTAACTGATGCATCAGTTTTGGAAT
CAAAAACTGAACTCGAAATTAAAATTATTCCAGATAAGACTGCCAAAACTCTTACACTCA
TTGATAGTGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAGGTACTATTGCTC
GTTCAGGTACTAAAAACTTTATGGAACAATTACAAAGTGGTGCCGCTGATATCAGTATGA
TTGGTCAATTTGGTGTTGGTTTCTACTCTGCTTACTTAGTTGCCGATACTGTTATTGTTC
ACTCTAAAAACAATGATGATGAACAATACGTTTGGGAATCAAGTGCTGGTGGTGAATTTA
CCATTGCTCTCGATCATACCGAACCATTAGGXXXXXXXXXXATTCATTGAAGGTCTCAAG
AAGAAGAATCTTGAAGTCATTTACATGTGTGACCCAATTGATGAATATGCTGTTCAACAA
TTAAAAGAATATGACGGTAAGAAATTAGTATCAATTACCAAAGAAGGTTTGAAATTAGAT
GAAACCGAAGATGAAAAGAAAAAAGCTGAACAAGATAAAGCTGCCAATGAAGAATTACTC
AAACAAGTTAAAGATGTATTGGGTGATAAAGTTGAAAAGGTAGTTCTCTCAACTCGTCTT
GCCAACTCACCATGTGTTTTAGTTACCTCTGAATATGGTTGGTCAGCAAACATGGAACGT
ATCATGAAAGCTCAAGCTCTCCGTGACTCTTCAATGTCATCATACATGTCATCAAAGAAA
ACTTTAGAATTAAATCCAGATCACCCAATCGTTAGAGATTTAGCCAAAAAAGCCGCCGAA
AAAAGTAAAACTTTCAAAGACTTTGTTTACTTATTATATGAAACTGCTCTCTTAACTTCA
GGTTTCTCATTAGATGAACCATCATCATTTGCTTCACGTATTCATCGTATGATTAAATTA
GGTTTAAGCATTCAAGATGATTCATCAGCAACAACTGAAGAATCAACAAACACAACAACC
AGTGATGATATTCCACCATTAGAAGAAAACGATGAACCATCTGAAATGGAAAAAGTCGAC
TAGATTTAATTACTAGCCCTTTTGCTATCAATTTTT

vlysnsn*FYLFFFVEHKKMAESQVERFTFQAEINQLMSLIINTFYSNKEVFLRELISNA
SDALDKIRYQSLTDASVLESKTELEIKIIPDKTAKTLTLIDSGIGMTKTDMVKNLGTIAR
SGTKNFMEQLQSGAADISMIGQFGVGFYSAYLVADTVIVHSKNNDDEQYVWESSAGGEFT
IALDHTEPL---

---FIEGLKKKNLEVIYMCDPIDEYAVQQLKEYDGKKLVSITKEGLKLDETEDEKKKAEQ
DKAANEELLKQVKDVLGDKVEKVVLSTRLANSPCVLVTSEYGWSANMERIMKAQALRDSS
MSSYMSSKKTLELNPDHPIVRDLAKKAAEKSKTFKDFVYLLYETALLTSGFSLDEPSSFA
SRIHRMIKLGLSIQDDSSATTEESTNTTTSDDIPPLEENDEPSEMEKVD*i*llalllsi
f

Frame A:
rsyililinficffl*nikkwlnhklnvshsklklislcplllilfiqikkyf*en*fqm
hlmp*ikfvtnh*lmhqfwnqklnsklklfqirlpkllhslivvlv*pkpiw*ri*vlll
vqvlktlwnnykvvplisv*lvnlvlvstllt*lpilllftlktmmmnntfgnqvlvvnl
pllsiipnh*---

---ih*rsqeees*shlhv*pn**iccstikri*r*eisinyqrrfeir*nrr*keks*t
r*scq*ritqts*rcig**s*kgsslnsscqltmcfsyl*iwlvskhgtyhessssp*lf
nviihvikenfriksrspnr*rfsqksrrkk*nfqrlcllii*ncslnfrflir*tiiic
ftyssyd*irfkhsr*fisnn*rinkhnnq**ystirrkr*ti*ngksrldlitspfain
f

Frame B:
gpif*f*lilfvffcrt*kng*its*tfhips*n*sayvpyy*yflfk*rsifkridfkc
i*clr*nslpiin*cisfgikn*trn*nysr*dcqnsyth**wywydqnrygkefryycs
fry*klygtitkwcr*yqydwsiwcwfllcllscrycycsl*kq***tirlgikcww*iy
hcsrsyrtir---

---FIEGLKKKNLEVIYMCDPIDEYAVQQLKEYDGKKLVSITKEGLKLDETEDEKKKAEQ
DKAANEELLKQVKDVLGDKVEKVVLSTRLANSPCVLVTSEYGWSANMERIMKAQALRDSS
MSSYMSSKKTLELNPDHPIVRDLAKKAAEKSKTFKDFVYLLYETALLTSGFSLDEPSSFA
SRIHRMIKLGLSIQDDSSATTEESTNTTTSDDIPPLEENDEPSEMEKVD*i*llalllsi
f

Frame C:
vlysnsn*FYLFFFVEHKKMAESQVERFTFQAEINQLMSLIINTFYSNKEVFLRELISNA
SDALDKIRYQSLTDASVLESKTELEIKIIPDKTAKTLTLIDSGIGMTKTDMVKNLGTIAR
SGTKNFMEQLQSGAADISMIGQFGVGFYSAYLVADTVIVHSKNNDDEQYVWESSAGGEFT
IALDHTEPL---

---slkvsrrrilksftcvtqlmnmlfnn*knmtvrn*yqlpkkv*n*mkpkmkrkklnk
iklpmknysnklkmywviklkr*fsqlvlpthhvf*lplnmvgqqtwnvs*klklsvtlq
chhtchqrkl*n*iqitqslei*pkkppkkvklsktlftyymklls*lqvsh*mnhhhll
hvfiv*ln*v*afkmihqqqlknqqtqqpvmifhh*kktmnhlkwkkstrfny*pfcyqf

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP202 (VHP202Q) /CSM/VH/VHP2-A/VHP202Q.Seq.d/                       1023   0.0
VHK153 (VHK153Q) /CSM/VH/VHK1-C/VHK153Q.Seq.d/                       1023   0.0
VHJ821 (VHJ821Q) /CSM/VH/VHJ8-A/VHJ821Q.Seq.d/                       1023   0.0
VHI607 (VHI607Q) /CSM/VH/VHI6-A/VHI607Q.Seq.d/                       1023   0.0
VHH735 (VHH735Q) /CSM/VH/VHH7-B/VHH735Q.Seq.d/                       1023   0.0
VHH731 (VHH731Q) /CSM/VH/VHH7-B/VHH731Q.Seq.d/                       1023   0.0
VHH708 (VHH708Q) /CSM/VH/VHH7-A/VHH708Q.Seq.d/                       1023   0.0
VHH272 (VHH272Q) /CSM/VH/VHH2-C/VHH272Q.Seq.d/                       1023   0.0
VHF303 (VHF303Q) /CSM/VH/VHF3-A/VHF303Q.Seq.d/                       1023   0.0
VHE854 (VHE854Q) /CSM/VH/VHE8-C/VHE854Q.Seq.d/                       1023   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L43591|L43591.1 Dictyostelium discoideum (clone C 6.1.1) heat shock cognate protein (hsc90) mRNA, complete cds.  1009   0.0    2
L34028|L34028.1 Plasmodium falciparum (clone HB3) heat shock protein 86 gene, complete cds.    98   1e-53  8
L34027|L34027.1 Plasmodium falciparum (clone Dd2) heat shock protein 86 gene, complete cds.    98   5e-53  9
Z29667|Z29667.1 P.falciparum (7) mRNA for heat-shock protein.      98   2e-51  8
AJ890081|AJ890081.1 Delia antiqua mRNA for Hsp90 protein.          72   1e-48  8
AF249998|AF249998.1 Habrotrocha constricta 82 kDa heat shock protein 2 (hsp82-2) gene, partial cds.    88   1e-46  4
AY122634|AY122634.1 Naegleria gruberi strain ATCC 30224 heat shock protein 90 (HSP-90) gene, partial cds.    86   2e-46  7
AF030694|AF030694.2 Plasmodium falciparum strain Dd2 heat shock protein 86 (HSP86), O1 (o1), O3 (o3), O2 (o2), CG8 (cg8), CG4 (cg4), CG3 (cg3), putative chloroquine resistance transporter (crt), CG9 (cg9), CG1 (cg1), CG6 (cg6), CG2 (cg2), and CG7 (cg7) genes, complete cds.    98   4e-46  9
AF165818|AF165818.4 Guillardia theta nucleomorph chromosome 1, complete sequence.    80   1e-45  8
AF143854|AF143854.1 Sinantherina socialis 82 kD heat shock protein (hsp82) gene, partial cds.    78   1e-42  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54651) RecName: Full=Heat shock cognate 90 kDa protein;             332   2e-89
L43591_1(L43591|pid:none) Dictyostelium discoideum (clone C 6.1....   332   2e-89
AB300196_1(AB300196|pid:none) Physarum polycephalum hsp90 mRNA f...   263   8e-69
FJ392027_1(FJ392027|pid:none) Portunus trituberculatus heat shoc...   258   4e-67
(P04810) RecName: Full=Heat shock protein 83; AltName: Full=HSP ...   256   1e-66
AY851367_1(AY851367|pid:none) Liriomyza huidobrensis heat shock ...   256   1e-66
U57467_1(U57467|pid:none) Drosophila melanogaster heat shock pro...   256   1e-66
(P04811) RecName: Full=Heat shock protein 83; AltName: Full=HSP ...   256   1e-66
U57459_1(U57459|pid:none) Drosophila melanogaster heat shock pro...   256   1e-66
EU306519_1(EU306519|pid:none) Mythimna separata Hsp90 mRNA, comp...   256   2e-66

 psg: 0.50  gvh: 0.46  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 24.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHB558 is cyt

>VHB558F.Seq
AGGTCCTATATTCTAATTCTAATTAATTTTATTTGTTTTTTTTTGTAGAACATAAAAAAA
TGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATCAGCTTATGTCCC
TTATTATTAATACTTTTTATTCAAATAAAGAAGTATTTTTAAGAGAATTGATTTCAAATG
CATCTGATGCCTTAGATAAAATTCGTTACCAATCATTAACTGATGCATCAGTTTTGGAAT
CAAAAACTGAACTCGAAATTAAAATTATTCCAGATAAGACTGCCAAAACTCTTACACTCA
TTGATAGTGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAGGTACTATTGCTC
GTTCAGGTACTAAAAACTTTATGGAACAATTACAAAGTGGTGCCGCTGATATCAGTATGA
TTGGTCAATTTGGTGTTGGTTTCTACTCTGCTTACTTAGTTGCCGATACTGTTATTGTTC
ACTCTAAAAACAATGATGATGAACAATACGTTTGGGAATCAAGTGCTGGTGGTGAATTTA
CCATTGCTCTCGATCATACCGAACCATTAGGNNNNNNNNNN

>VHB558Z.Seq
NNNNNNNNNNATTCATTGAAGGTCTCAAGAAGAAGAATCTTGAAGTCATTTACATGTGTG
ACCCAATTGATGAATATGCTGTTCAACAATTAAAAGAATATGACGGTAAGAAATTAGTAT
CAATTACCAAAGAAGGTTTGAAATTAGATGAAACCGAAGATGAAAAGAAAAAAGCTGAAC
AAGATAAAGCTGCCAATGAAGAATTACTCAAACAAGTTAAAGATGTATTGGGTGATAAAG
TTGAAAAGGTAGTTCTCTCAACTCGTCTTGCCAACTCACCATGTGTTTTAGTTACCTCTG
AATATGGTTGGTCAGCAAACATGGAACGTATCATGAAAGCTCAAGCTCTCCGTGACTCTT
CAATGTCATCATACATGTCATCAAAGAAAACTTTAGAATTAAATCCAGATCACCCAATCG
TTAGAGATTTAGCCAAAAAAGCCGCCGAAAAAAGTAAAACTTTCAAAGACTTTGTTTACT
TATTATATGAAACTGCTCTCTTAACTTCAGGTTTCTCATTAGATGAACCATCATCATTTG
CTTCACGTATTCATCGTATGATTAAATTAGGTTTAAGCATTCAAGATGATTCATCAGCAA
CAACTGAAGAATCAACAAACACAACAACCAGTGATGATATTCCACCATTAGAAGAAAACG
ATGAACCATCTGAAATGGAAAAAGTCGACTAGATTTAATTACTAGCCCTTTTGCTATCAA
TTTTT

>VHB558P.Seq
AGGTCCTATATTCTAATTCTAATTAATTTTATTTGTTTTTTTTTGTAGAACATAAAAAAA
TGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATCAGCTTATGTCCC
TTATTATTAATACTTTTTATTCAAATAAAGAAGTATTTTTAAGAGAATTGATTTCAAATG
CATCTGATGCCTTAGATAAAATTCGTTACCAATCATTAACTGATGCATCAGTTTTGGAAT
CAAAAACTGAACTCGAAATTAAAATTATTCCAGATAAGACTGCCAAAACTCTTACACTCA
TTGATAGTGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAGGTACTATTGCTC
GTTCAGGTACTAAAAACTTTATGGAACAATTACAAAGTGGTGCCGCTGATATCAGTATGA
TTGGTCAATTTGGTGTTGGTTTCTACTCTGCTTACTTAGTTGCCGATACTGTTATTGTTC
ACTCTAAAAACAATGATGATGAACAATACGTTTGGGAATCAAGTGCTGGTGGTGAATTTA
CCATTGCTCTCGATCATACCGAACCATTAGG----------ATTCATTGAAGGTCTCAAG
AAGAAGAATCTTGAAGTCATTTACATGTGTGACCCAATTGATGAATATGCTGTTCAACAA
TTAAAAGAATATGACGGTAAGAAATTAGTATCAATTACCAAAGAAGGTTTGAAATTAGAT
GAAACCGAAGATGAAAAGAAAAAAGCTGAACAAGATAAAGCTGCCAATGAAGAATTACTC
AAACAAGTTAAAGATGTATTGGGTGATAAAGTTGAAAAGGTAGTTCTCTCAACTCGTCTT
GCCAACTCACCATGTGTTTTAGTTACCTCTGAATATGGTTGGTCAGCAAACATGGAACGT
ATCATGAAAGCTCAAGCTCTCCGTGACTCTTCAATGTCATCATACATGTCATCAAAGAAA
ACTTTAGAATTAAATCCAGATCACCCAATCGTTAGAGATTTAGCCAAAAAAGCCGCCGAA
AAAAGTAAAACTTTCAAAGACTTTGTTTACTTATTATATGAAACTGCTCTCTTAACTTCA
GGTTTCTCATTAGATGAACCATCATCATTTGCTTCACGTATTCATCGTATGATTAAATTA
GGTTTAAGCATTCAAGATGATTCATCAGCAACAACTGAAGAATCAACAAACACAACAACC
AGTGATGATATTCCACCATTAGAAGAAAACGATGAACCATCTGAAATGGAAAAAGTCGAC
TAGATTTAATTACTAGCCCTTTTGCTATCAATTTTT