>VHB557 (VHB557Q) /CSM/VH/VHB5-C/VHB557Q.Seq.d/
AGCAAATCCAAAAGAATGACAACTACTAATTGGAGTTTAAAAGTTGATAGAGGCAGACAA
ACTTGGGAGTACTCTCAAGAGAAAAAAGAAGCAACAGATGTTGATATTCATTTATTAAGA
TTAAAAGAACCAGGAACACATTGTCCAGAAGGATGTGATTTAAATAGAGCAAAAACACCA
CAACAAGCAATTAAAAAAGCATTCCAATATTTCTCAAAAGTTCAAACTGAGGATGGTCAT
TGGGCAGGTGATTATGGTGGTCCAATGTTTTTATTACCAGGTTTAGTCATTACATGTTAT
GTAACAGGTTATCAATTACCAGAATCAACTCAAAGAGAAATCATTAGATATTTATTCAAT
AGACAAAATCCAGTTGATGGTGGTTGGGTCACATTGAGGCACATTCAGATATTTTTGGAA
CAACATTACAATATGTTTCATTAAGATTATTAGGAGTACCAGCAGATCATCCATCAGTAG
TAAAAGCAAGAACATTTTTATTACAAAATGGTGGTGCAACAGGTATTCCATCATGGGGTA
AATTTTGGTTAGCAACATTAAATGCTTATGATTGGAATGGTTTAAATCXXXXXXXXXXTC
AAATCAAATGTTACCAAAGAATACGTTCAACATGAAACTTCACAAGTTGTAAACACTGGT
TGGGCTTTACTCTCACTCATGTCTGCTAAATATCCAGATAGAGAATGCATTGAAAGAGGT
ATTAAATTCCTCATACAAAGACAATATCCAAATGGTGATTTCCCACAAGAATCTATTATT
GGTGTTTTCAATTTCAATTGTATGATCAGTTATTCAAATTATAAAAATATTTTCCCATTA
TGGGCTTTATCTAGATATAATCAATTATATTTAAAATCTAAAATTTAAATATTTCTAAAA
AAAACAAATATATTAAAAAAATTCTATTAATAATAATAATAATTTTAAAAAATATAATAA
TAACAATAATAGTTTAAAAATAATAAATAATAATAATAATAATAATAATAATAGTAAAAC
ATAAATATAAATAATAATAGTAATATGAATAGCAATAATAATAATAATAATTATAATAAT
AATAATAATAAGTCGATTATAAAATAATAATAATAGTAATAATAATAGTAATAATAATAG
TAATAATAATTTTTAGGTAAAAGTTTGTTCATTTATATGGTGTTTAATAAAACATTATAT
CGACAGTGCAACTAATTTTTTTATTATTGTTATTATTACTATTTTTATTGTTAATTATTT
NCTTATATGTAATTCAATAAACTCATTATCATTTATTATATTATTNNCACACACAC

qiqkndny*lefks**rqtnlgvlsrekrsnrc*ysfikikrtrntlsrrm*fk*skntt
tsn*ksipiflkssn*gwslgr*lwwsnvfitrfshymlcnrlsitrinskrnh*ifiq*
tkss*WWLGHIEAHSDIFGTTLQYVSLRLLGVPADHPSVVKARTFLLQNGGATGIPSWGK
FWLATLNAYDWNGLN---

---KSNVTKEYVQHETSQVVNTGWALLSLMSAKYPDRECIERGIKFLIQRQYPNGDFPQE
SIIGVFNFNCMISYSNYKNIFPLWALSRYNQLYLKSKI*iflkktnilkkfy*****f*k
i***q**fknnk**********nininnnsnmnsnnnnnnynnnnnksiik*********
******flgkslfiymvfnktlyrqcn*ffyycyyyyfyc*lfxym*fnkliiiyyiixt
h

Frame A:
skskrmtttnwslkvdrgrqtweysqekkeatdvdihllrlkepgthcpegcdlnraktp
qqaikkafqyfskvqtedghwagdyggpmfllpglvitcyvtgyqlpestqreiirylfn
rqnpvdggwvtlrhiqifleqhynmfh*dy*eyqqiihq**kqehfyykmvvqqvfhhgv
nfg*qh*mlmigmv*i---

---snqmlpkntfnmklhkl*tlvglyshscllniqienalkevlnssykdniqmvishk
nlllvfsisiv*sviqiikifshyglyldiinyi*nlkfkyf*kkqiy*knsinnnnnfk
kynnnnnslkiinnnnnnnnnskt*i*iiivi*iaiiiiiiiiiiiisrl*nnnnsnnns
nnnsnnnf*vkvcsfiwclikhyidsatnffiiviitifivnyxlicnsinslsfiilxx
ht

Frame B:
anpke*qlligv*klieadklgstlkrkkkqqmlifiy*d*knqehivqkdvi*ieqkhh
nkqlkkhsnisqkfklrmvigqvimvvqcfyyqv*slhvm*qvinyqnqlkeksldiysi
dkiqlmvvgsh*gtfryfwnniticfikiirstsrssissksknifitkwwcnrysimg*
ilvsnikcl*lewfk---

---qikcyqrirst*nftsckhwlgftlthvc*isr*rmh*kry*iphtktiskw*fptr
iyywcfqfqlydqlfkl*kyfpimgfi*i*siifki*nlniskknkyikkilliiiiilk
niiitiiv*k**iiiiiiiiivkhkyk****ye*q*****l******vdykiiiiviiiv
iiiviiifr*kfvhlygv**niistvqliflllllllfllliixlyviq*thyhllyyxh
th

Frame C:
qiqkndny*lefks**rqtnlgvlsrekrsnrc*ysfikikrtrntlsrrm*fk*skntt
tsn*ksipiflkssn*gwslgr*lwwsnvfitrfshymlcnrlsitrinskrnh*ifiq*
tkss*WWLGHIEAHSDIFGTTLQYVSLRLLGVPADHPSVVKARTFLLQNGGATGIPSWGK
FWLATLNAYDWNGLN---

---KSNVTKEYVQHETSQVVNTGWALLSLMSAKYPDRECIERGIKFLIQRQYPNGDFPQE
SIIGVFNFNCMISYSNYKNIFPLWALSRYNQLYLKSKI*iflkktnilkkfy*****f*k
i***q**fknnk**********nininnnsnmnsnnnnnnynnnnnksiik*********
******flgkslfiymvfnktlyrqcn*ffyycyyyyfyc*lfxym*fnkliiiyyiixt
h

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB557 (VHB557Q) /CSM/VH/VHB5-C/VHB557Q.Seq.d/                       1667   0.0
VHP346 (VHP346Q) /CSM/VH/VHP3-B/VHP346Q.Seq.d/                       1140   0.0
VHM553 (VHM553Q) /CSM/VH/VHM5-C/VHM553Q.Seq.d/                       1140   0.0
VHL721 (VHL721Q) /CSM/VH/VHL7-A/VHL721Q.Seq.d/                       1140   0.0
VHJ519 (VHJ519Q) /CSM/VH/VHJ5-A/VHJ519Q.Seq.d/                       1140   0.0
VHJ385 (VHJ385Q) /CSM/VH/VHJ3-D/VHJ385Q.Seq.d/                       1140   0.0
VHI438 (VHI438Q) /CSM/VH/VHI4-B/VHI438Q.Seq.d/                       1140   0.0
VHH493 (VHH493Q) /CSM/VH/VHH4-D/VHH493Q.Seq.d/                       1140   0.0
VHH284 (VHH284Q) /CSM/VH/VHH2-D/VHH284Q.Seq.d/                       1140   0.0
VHE637 (VHE637Q) /CSM/VH/VHE6-B/VHE637Q.Seq.d/                       1140   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF159949|AF159949.1 Dictyostelium discoideum cycloartenol synthase (CAS1) mRNA, partial cds.   737   0.0    2
AY520819|AY520819.1 Centella asiatica cycloartenol synthase (OSCCCS) mRNA, complete cds.    60   5e-05  2
EC757725|EC757725.1 PPE00000938 Agencourt Biosciences Agen-0020 Non-normalized library cloned into Not I - Eco RV sites of pAGEN-1 Physarum polycephalum cDNA, mRNA sequence.    58   6e-05  2
EB329833|EB329833.1 CNSN01-F-109865-501 Normalized CNS library (juvenile 1) Aplysia californica cDNA clone CNSN01-F-109865 5', mRNA sequence.    58   7e-04  1
EB303482|EB303482.1 CNSN01-F-062558-501 Normalized CNS library (juvenile 1) Aplysia californica cDNA clone CNSN01-F-062558 5', mRNA sequence.    58   7e-04  1
CF604686|CF604686.1 RADIC01_000182 Grape Root pSPORT1 Library Vitis vinifera cDNA 5', mRNA sequence.    56   0.003  1
BY180927|BY180927.1 Mus musculus NOD-derived CD11c +ve dendritic cells cDNA, RIKEN full-length enriched library, clone:F630043C20, 5' end partial sequence.    56   0.003  1
AB009029|AB009029.1 Panax ginseng OSCPNX1 mRNA for Cycloartenol Synthase, complete cds.    46   0.003  2
DY672217|DY672217.1 STRCQ62JX cold-stressed Fragaria vesca seedlings cDNA library Fragaria vesca cDNA clone STRCQ62, mRNA sequence.    54   0.010  1
DW495509|DW495509.1 GH_RMIRS_108_A12_R Cotton Normalized Library random primed Gossypium hirsutum cDNA, mRNA sequence.    54   0.010  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55D85) RecName: Full=Cycloartenol synthase;          EC=5.4.99...   270   1e-70
(P84466) RecName: Full=Lanosterol synthase;          EC=5.4.99.7...   108   1e-43
AK044016_1(AK044016|pid:none) Mus musculus 10 days neonate corte...   105   3e-42
AK312489_1(AK312489|pid:none) Homo sapiens cDNA, FLJ92849, highl...   112   8e-42
BC035638_1(BC035638|pid:none) Homo sapiens lanosterol synthase (...   112   8e-42
AJ239031_1(AJ239031|pid:none) Homo sapiens LSS gene, partial, ex...   111   8e-42
(P48450) RecName: Full=Lanosterol synthase;          EC=5.4.99.7...   108   2e-41
GN121451_1(GN121451|pid:none) Sequence 4347 from Patent WO200903...   108   8e-41
BX255930_10(BX255930|pid:none) Zebrafish DNA sequence from clone...   112   1e-40
BC134214_1(BC134214|pid:none) Danio rerio lanosterol synthase (2...   112   1e-40

 psg: 0.75  gvh: 0.53  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.30  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VHB557 is cyt

>VHB557F.Seq
AGCAAATCCAAAAGAATGACAACTACTAATTGGAGTTTAAAAGTTGATAGAGGCAGACAA
ACTTGGGAGTACTCTCAAGAGAAAAAAGAAGCAACAGATGTTGATATTCATTTATTAAGA
TTAAAAGAACCAGGAACACATTGTCCAGAAGGATGTGATTTAAATAGAGCAAAAACACCA
CAACAAGCAATTAAAAAAGCATTCCAATATTTCTCAAAAGTTCAAACTGAGGATGGTCAT
TGGGCAGGTGATTATGGTGGTCCAATGTTTTTATTACCAGGTTTAGTCATTACATGTTAT
GTAACAGGTTATCAATTACCAGAATCAACTCAAAGAGAAATCATTAGATATTTATTCAAT
AGACAAAATCCAGTTGATGGTGGTTGGGTCACATTGAGGCACATTCAGATATTTTTGGAA
CAACATTACAATATGTTTCATTAAGATTATTAGGAGTACCAGCAGATCATCCATCAGTAG
TAAAAGCAAGAACATTTTTATTACAAAATGGTGGTGCAACAGGTATTCCATCATGGGGTA
AATTTTGGTTAGCAACATTAAATGCTTATGATTGGAATGGTTTAAATCNNNNNNNNNN

>VHB557Z.Seq
NNNNNNNNNNTCAAATCAAATGTTACCAAAGAATACGTTCAACATGAAACTTCACAAGTT
GTAAACACTGGTTGGGCTTTACTCTCACTCATGTCTGCTAAATATCCAGATAGAGAATGC
ATTGAAAGAGGTATTAAATTCCTCATACAAAGACAATATCCAAATGGTGATTTCCCACAA
GAATCTATTATTGGTGTTTTCAATTTCAATTGTATGATCAGTTATTCAAATTATAAAAAT
ATTTTCCCATTATGGGCTTTATCTAGATATAATCAATTATATTTAAAATCTAAAATTTAA
ATATTTCTAAAAAAAACAAATATATTAAAAAAATTCTATTAATAATAATAATAATTTTAA
AAAATATAATAATAACAATAATAGTTTAAAAATAATAAATAATAATAATAATAATAATAA
TAATAGTAAAACATAAATATAAATAATAATAGTAATATGAATAGCAATAATAATAATAAT
AATTATAATAATAATAATAATAAGTCGATTATAAAATAATAATAATAGTAATAATAATAG
TAATAATAATAGTAATAATAATTTTTAGGTAAAAGTTTGTTCATTTATATGGTGTTTAAT
AAAACATTATATCGACAGTGCAACTAATTTTTTTATTATTGTTATTATTACTATTTTTAT
TGTTAATTATTTNCTTATATGTAATTCAATAAACTCATTATCATTTATTATATTATTNNC
ACACACAC

>VHB557P.Seq
AGCAAATCCAAAAGAATGACAACTACTAATTGGAGTTTAAAAGTTGATAGAGGCAGACAA
ACTTGGGAGTACTCTCAAGAGAAAAAAGAAGCAACAGATGTTGATATTCATTTATTAAGA
TTAAAAGAACCAGGAACACATTGTCCAGAAGGATGTGATTTAAATAGAGCAAAAACACCA
CAACAAGCAATTAAAAAAGCATTCCAATATTTCTCAAAAGTTCAAACTGAGGATGGTCAT
TGGGCAGGTGATTATGGTGGTCCAATGTTTTTATTACCAGGTTTAGTCATTACATGTTAT
GTAACAGGTTATCAATTACCAGAATCAACTCAAAGAGAAATCATTAGATATTTATTCAAT
AGACAAAATCCAGTTGATGGTGGTTGGGTCACATTGAGGCACATTCAGATATTTTTGGAA
CAACATTACAATATGTTTCATTAAGATTATTAGGAGTACCAGCAGATCATCCATCAGTAG
TAAAAGCAAGAACATTTTTATTACAAAATGGTGGTGCAACAGGTATTCCATCATGGGGTA
AATTTTGGTTAGCAACATTAAATGCTTATGATTGGAATGGTTTAAATC----------TC
AAATCAAATGTTACCAAAGAATACGTTCAACATGAAACTTCACAAGTTGTAAACACTGGT
TGGGCTTTACTCTCACTCATGTCTGCTAAATATCCAGATAGAGAATGCATTGAAAGAGGT
ATTAAATTCCTCATACAAAGACAATATCCAAATGGTGATTTCCCACAAGAATCTATTATT
GGTGTTTTCAATTTCAATTGTATGATCAGTTATTCAAATTATAAAAATATTTTCCCATTA
TGGGCTTTATCTAGATATAATCAATTATATTTAAAATCTAAAATTTAAATATTTCTAAAA
AAAACAAATATATTAAAAAAATTCTATTAATAATAATAATAATTTTAAAAAATATAATAA
TAACAATAATAGTTTAAAAATAATAAATAATAATAATAATAATAATAATAATAGTAAAAC
ATAAATATAAATAATAATAGTAATATGAATAGCAATAATAATAATAATAATTATAATAAT
AATAATAATAAGTCGATTATAAAATAATAATAATAGTAATAATAATAGTAATAATAATAG
TAATAATAATTTTTAGGTAAAAGTTTGTTCATTTATATGGTGTTTAATAAAACATTATAT
CGACAGTGCAACTAATTTTTTTATTATTGTTATTATTACTATTTTTATTGTTAATTATTT
NCTTATATGTAATTCAATAAACTCATTATCATTTATTATATTATTNNCACACACAC