>VHB552 (VHB552Q) /CSM/VH/VHB5-C/VHB552Q.Seq.d/
XXXXXXXXXXAANTGGTAATNTTGATGNGAGATCAACATNTTTCGNTAAACAATNGTTAA
TCCNNTNAATGTAGGCNACTTTCTCTTNATCGGAATAGNAATGTTGGACACCAGANGCTG
TTGAACCAGAATAACCACCAATGGTATNGACAGCGCCA

---xvxlmxdqhxsxnnx*SXXCRXLSLXRNXNVGHQXLLNQNNHQWYXQR

Frame A:
---xgnxdxrstxfxkqxlixxm*atfsxse*xcwtpxavepe*ppmvxtap


Frame B:
---xvxlmxdqhxsxnnx*SXXCRXLSLXRNXNVGHQXLLNQNNHQWYXQR


Frame C:
---w*x*xeinxfr*txvnpxnvgxflxigixmldtrxc*trittngxdsa

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB552 (VHB552Q) /CSM/VH/VHB5-C/VHB552Q.Seq.d/                        210   4e-54
AHK247 (AHK247Q) /CSM/AH/AHK2-B/AHK247Q.Seq.d/                        190   4e-48
VFK156 (VFK156Q) /CSM/VF/VFK1-C/VFK156Q.Seq.d/                        186   6e-47
AFI128 (AFI128Q) /CSM/AF/AFI1-B/AFI128Q.Seq.d/                        168   1e-41
SLK603 (SLK603Q) /CSM/SL/SLK6-A/SLK603Q.Seq.d/                         30   8.8

 ***** No hits found ******

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 716260
Number of Hits to DB: 835,385
Number of extensions: 22212
Number of successful extensions: 1079
Number of sequences better than 10.0: 0
Length of query: 158
Length of database: 1,729,687,253
Length adjustment: 19
Effective length of query: 139
Effective length of database: 1,716,078,313
Effective search space: 238534885507
Effective search space used: 238534885507
X1: 11 (21.8 bits)
S2: 18 (36.2 bits)

ANTI-DNA BLAST search was processed by blast@nig.ac.jp, National Institute of Genetics, Japan.

------=_Part_0_8984226.1175668384143--

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

L36202_1(L36202|pid:none) Dictyostelium discoideum fimbrin (fimA...    76   4e-13
(P54680) RecName: Full=Fimbrin;                                        76   4e-13
BC063742_1(BC063742|pid:none) Danio rerio plastin 1 (I isoform),...    42   0.005
(Q3V0K9) RecName: Full=Plastin-1;  &AK133070_1(AK133070|pid:none...    42   0.009
BT049835_1(BT049835|pid:none) Salmo salar clone ssal-eve-569-377...    41   0.011
(A6H742) RecName: Full=Plastin-1;  &BC146105_1(BC146105|pid:none)      40   0.025
AJ719624_1(AJ719624|pid:none) Gallus gallus mRNA for hypothetica...    40   0.025
CR858800_1(CR858800|pid:none) Pongo abelii mRNA; cDNA DKFZp459F2...    39   0.043
BC031083_1(BC031083|pid:none) Homo sapiens plastin 1 (I isoform)...    39   0.043
(Q14651) RecName: Full=Plastin-1; AltName: Full=Intestine-specif...    39   0.043

 psg: 0.73  gvh: 0.31  alm: 0.65  top: 0.53  tms: 0.00  mit: 0.51  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: mitochondrial
	 20.0 %: nuclear
	  8.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for VHB552 is mit

>VHB552Z.Seq
NNNNNNNNNNAANTGGTAATNTTGATGNGAGATCAACATNTTTCGNTAAACAATNGTTAA
TCCNNTNAATGTAGGCNACTTTCTCTTNATCGGAATAGNAATGTTGGACACCAGANGCTG
TTGAACCAGAATAACCACCAATGGTATNGACAGCGCCA