>VHB531 (VHB531Q) /CSM/VH/VHB5-B/VHB531Q.Seq.d/
AGTAACAATCAAATATAAGATTTCTTATATTGGATACGTGGTGTACTTTTGTGTTTGGGT
GGATTTTATTATTTATTTATTTTAATCATCATTTTTATTTGTAATATATTTCATTTTTAT
CACACAAAACACACACAAAACACACACTCACAAAAACACACACTCACACATAAATAAATA
CACAATAATAAAAGAAGAAAGAAAATGGAGAAAAGAAAGAGTATATTTTTTGCAAATGAT
AGTGAAGATAATACATCATCAGGAGATTTAAGTAGTTTTACAAAACGTGGTACAATTACA
CCAGGTAAGATTGCAAATGCACGTGAAGGAGAGTTATCACAAATGATTGATGTTGAATCG
GAACAGAAATATCAAGAAATTATTGAACTTTTAGTTACAGGTGGTTATTTTAGAGCACGT
ATTTCAGGTTTATCACCATTTGATAAAGTTGTTGGTGGCATGGCTTGGTCAATCACTGCT
TCAAATGTTGATGTTGACGCTGATTTATTCTTTCAAGAAAATTCAAATATTAAACAAAAA
GTATCATTATCAGAAGAATTAATTAAAGCATTAAATCGTATGAAATGTCCATTTCCATTA
CAAGCACAACATATTACATTATTGAATTATATAACATTATTTXXXXXXXXXXAACGTCAA
ATGGTAGAATATAAACAAAAGGTCGAACAACTCTTATCACAAGATGCCTCTACTCAAGAG
GATCTTCAAGACAGTGATAGACAACAACAAATTCAACAAGCATTCGATAGTGATATTGGT
AAATTAAAGAAATTGAAATTATTACTCAATAAAAAGAATAGAGATATCTCTTTGATTCAA
CGTTATCTCGATGAATTACCATCTCGTGCCGAACTATTACAATATCAAAGACAATTTGTT
GAATTATCCGAACAAAGTAGTTCGAAATTAACCGAAACCAGACAATACTATACAACCTAC
AATACCTTTGAAGATAAGAAAACACTTTTAGAAAATGAACTCTCAATTCTCAATTCAATT
CAATCCAAATATAGCATAGCTATGTCTTCAACAAATAATAAAGAATTATTCATTAAATCA
ATGGATCAAATCATTGATGGTATTCAACAAGTTTTAGATAAATCTGAAAATAAATGGAAT
GTTGAAAAAGTTCGTCATGATACTTTATCTGATCAATATATGACTTTATTAGATCAAGAG
AGAAATTATTATAAATTAACAAAAGACTTGAAGAAGAATGTAGAAAGAANAATTATTAAT
TAAAAAATT

snnqi*dflywirgvllclggfyylfiliiificnifhfyhtkhtqnthsqkhtlthk*I
HNNKRRKKMEKRKSIFFANDSEDNTSSGDLSSFTKRGTITPGKIANAREGELSQMIDVES
EQKYQEIIELLVTGGYFRARISGLSPFDKVVGGMAWSITASNVDVDADLFFQENSNIKQK
VSLSEELIKALNRMKCPFPLQAQHITLLNYITLF---

---RQMVEYKQKVEQLLSQDASTQEDLQDSDRQQQIQQAFDSDIGKLKKLKLLLNKKNRD
ISLIQRYLDELPSRAELLQYQRQFVELSEQSSSKLTETRQYYTTYNTFEDKKTLLENELS
ILNSIQSKYSIAMSSTNNKELFIKSMDQIIDGIQQVLDKSENKWNVEKVRHDTLSDQYMT
LLDQERNYYKLTKDLKKNVERXIIN*ki

Frame A:
snnqi*dflywirgvllclggfyylfiliiificnifhfyhtkhtqnthsqkhtlthk*I
HNNKRRKKMEKRKSIFFANDSEDNTSSGDLSSFTKRGTITPGKIANAREGELSQMIDVES
EQKYQEIIELLVTGGYFRARISGLSPFDKVVGGMAWSITASNVDVDADLFFQENSNIKQK
VSLSEELIKALNRMKCPFPLQAQHITLLNYITLF---

---nvkw*ninkrsnnsyhkmpllkrifktvidnnkfnkhsivilvn*rn*nyysikrie
isl*fnvismnyhlvpnyynikdnllnypnkvvrn*pkpdntiqptiplkirkhf*kmns
qfsiqfnpnia*lclqqiiknyslnqwikslmvfnkf*inlkingmlkkfvmilylini*
ly*ikreiiin*qkt*rrm*kexllikk

Frame B:
vtikykisyigyvvyfcvwvdfiiylf*ssflfviyfifitqnthkththknthshinky
tiikeerkwrkervyflqmivkiihhqei*vvlqnvvqlhqvrlqmhvkesyhk*lmlnr
nrnikkllnf*lqvvilehvfqvyhhlikllvawlgqsllqmlmltliysfkkiqilnkk
yhyqkn*lkh*iv*nvhfhykhnilhy*ii*hy---

---tsngri*tkgrttlitrclysrgssrq**tttnstsir**yw*ikeieiitq*ke*r
ylfdstlsr*itiscrtitisktic*iirtk*feinrnqtilynlqyl*r*entfrk*tl
nsqfnsiqi*hsyvfnk**riih*ingsnh*wystsfr*i*k*mec*ksss*yfi*siyd
firsrekll*inkrleeecrkxny*lkn

Frame C:
*qsnirflildtwctfvfgwillfiyfnhhfyl*yisflshkthtkhtltkththt*int
q**kkkengekkeyifck***r*yiirrfk*fyktwynytr*dckct*rrvitnd*c*ig
teisrny*tfsyrwlf*styfrfiti**scwwhglvnhcfkc*c*r*filsrkfky*tks
iiirrin*siksyemsisitsttyyiielynii---

---RQMVEYKQKVEQLLSQDASTQEDLQDSDRQQQIQQAFDSDIGKLKKLKLLLNKKNRD
ISLIQRYLDELPSRAELLQYQRQFVELSEQSSSKLTETRQYYTTYNTFEDKKTLLENELS
ILNSIQSKYSIAMSSTNNKELFIKSMDQIIDGIQQVLDKSENKWNVEKVRHDTLSDQYMT
LLDQERNYYKLTKDLKKNVERXIIN*ki

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB531 (VHB531Q) /CSM/VH/VHB5-B/VHB531Q.Seq.d/                       2014   0.0
VHO250 (VHO250Q) /CSM/VH/VHO2-C/VHO250Q.Seq.d/                       1209   0.0
VHB221 (VHB221Q) /CSM/VH/VHB2-A/VHB221Q.Seq.d/                       1209   0.0
VHA391 (VHA391Q) /CSM/VH/VHA3-D/VHA391Q.Seq.d/                       1209   0.0
SFC233 (SFC233Q) /CSM/SF/SFC2-B/SFC233Q.Seq.d/                       1201   0.0
VFH882 (VFH882Q) /CSM/VF/VFH8-D/VFH882Q.Seq.d/                       1187   0.0
VHJ120 (VHJ120Q) /CSM/VH/VHJ1-A/VHJ120Q.Seq.d/                        898   0.0
VHH305 (VHH305Q) /CSM/VH/VHH3-A/VHH305Q.Seq.d/                        837   0.0
VSH550 (VSH550Q) /CSM/VS/VSH5-C/VSH550Q.Seq.d/                        668   0.0
VHN779 (VHN779Q) /CSM/VH/VHN7-D/VHN779Q.Seq.d/                        656   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC006905|AC006905.1 Caenorhabditis elegans clone Y74E4, *** SEQUENCING IN PROGRESS ***,2 unordered pieces.    44   0.23   1
AC006279|AC006279.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    38   0.49   5
AE006418|AE006418.1 Lactococcus lactis subsp. lactis IL1403 section 180 of 218 of the complete genome.    32   0.50   4
AC002036|AC002036.1 Homo sapiens Chromosome 11q23 PAC clone pDJ356d6, complete sequence.    42   0.67   3
AC002290|AC002290.2 Homo sapiens chromosome 11 clone pDJ105h16, *** SEQUENCING IN PROGRESS ***, 20 unordered pieces.    42   0.89   1
AC006436|AC006436.5 Arabidopsis thaliana chromosome 2 clone F13J11 map mi398, complete sequence.    42   0.89   1
AC006218|AC006218.4 Arabidopsis thaliana chromosome 2 clone F17L24 map GPC6, complete sequence.    42   0.89   1
AB003310|AB003310.1 Candida albicans DNA for chitin synthase regulatory factor, complete cds.    42   0.89   1
AC146661|AC146661.3 Callithrix jacchus clone CH259-140E16, complete sequence.    42   0.89   1
AC012394|AC012394.3 Arabidopsis thaliana chromosome 1 BAC F15M4 genomic sequence, complete sequence.    38   1.1    2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54CV3) RecName: Full=Coiled-coil domain-containing protein 93 ...   301   3e-80
BC115269_1(BC115269|pid:none) Danio rerio coiled-coil domain con...   144   9e-33
BC134535_1(BC134535|pid:none) Bos taurus hypothetical LOC616839,...   140   1e-31
AM393188_1(AM393188|pid:none) Synthetic construct Homo sapiens c...   139   2e-31
AK159415_1(AK159415|pid:none) Mus musculus osteoclast-like cell ...   139   2e-31
AK085701_1(AK085701|pid:none) Mus musculus 10 days lactation, ad...   139   2e-31
BC024674_1(BC024674|pid:none) Mus musculus coiled-coil domain co...   139   2e-31
AB384355_1(AB384355|pid:none) Synthetic construct DNA, clone: pF...   139   2e-31
(Q5BJT7) RecName: Full=Coiled-coil domain-containing protein 93;...   139   2e-31
(Q640U7) RecName: Full=Coiled-coil domain-containing protein 93;...   139   3e-31

 psg: 0.58  gvh: 0.30  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.03
 nuc: 0.08  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 24.0 %: mitochondrial
	 16.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VHB531 is nuc

>VHB531F.Seq
AGTAACAATCAAATATAAGATTTCTTATATTGGATACGTGGTGTACTTTTGTGTTTGGGT
GGATTTTATTATTTATTTATTTTAATCATCATTTTTATTTGTAATATATTTCATTTTTAT
CACACAAAACACACACAAAACACACACTCACAAAAACACACACTCACACATAAATAAATA
CACAATAATAAAAGAAGAAAGAAAATGGAGAAAAGAAAGAGTATATTTTTTGCAAATGAT
AGTGAAGATAATACATCATCAGGAGATTTAAGTAGTTTTACAAAACGTGGTACAATTACA
CCAGGTAAGATTGCAAATGCACGTGAAGGAGAGTTATCACAAATGATTGATGTTGAATCG
GAACAGAAATATCAAGAAATTATTGAACTTTTAGTTACAGGTGGTTATTTTAGAGCACGT
ATTTCAGGTTTATCACCATTTGATAAAGTTGTTGGTGGCATGGCTTGGTCAATCACTGCT
TCAAATGTTGATGTTGACGCTGATTTATTCTTTCAAGAAAATTCAAATATTAAACAAAAA
GTATCATTATCAGAAGAATTAATTAAAGCATTAAATCGTATGAAATGTCCATTTCCATTA
CAAGCACAACATATTACATTATTGAATTATATAACATTATTTNNNNNNNNNN

>VHB531Z.Seq
NNNNNNNNNNAACGTCAAATGGTAGAATATAAACAAAAGGTCGAACAACTCTTATCACAA
GATGCCTCTACTCAAGAGGATCTTCAAGACAGTGATAGACAACAACAAATTCAACAAGCA
TTCGATAGTGATATTGGTAAATTAAAGAAATTGAAATTATTACTCAATAAAAAGAATAGA
GATATCTCTTTGATTCAACGTTATCTCGATGAATTACCATCTCGTGCCGAACTATTACAA
TATCAAAGACAATTTGTTGAATTATCCGAACAAAGTAGTTCGAAATTAACCGAAACCAGA
CAATACTATACAACCTACAATACCTTTGAAGATAAGAAAACACTTTTAGAAAATGAACTC
TCAATTCTCAATTCAATTCAATCCAAATATAGCATAGCTATGTCTTCAACAAATAATAAA
GAATTATTCATTAAATCAATGGATCAAATCATTGATGGTATTCAACAAGTTTTAGATAAA
TCTGAAAATAAATGGAATGTTGAAAAAGTTCGTCATGATACTTTATCTGATCAATATATG
ACTTTATTAGATCAAGAGAGAAATTATTATAAATTAACAAAAGACTTGAAGAAGAATGTA
GAAAGAANAATTATTAATTAAAAAATT

>VHB531P.Seq
AGTAACAATCAAATATAAGATTTCTTATATTGGATACGTGGTGTACTTTTGTGTTTGGGT
GGATTTTATTATTTATTTATTTTAATCATCATTTTTATTTGTAATATATTTCATTTTTAT
CACACAAAACACACACAAAACACACACTCACAAAAACACACACTCACACATAAATAAATA
CACAATAATAAAAGAAGAAAGAAAATGGAGAAAAGAAAGAGTATATTTTTTGCAAATGAT
AGTGAAGATAATACATCATCAGGAGATTTAAGTAGTTTTACAAAACGTGGTACAATTACA
CCAGGTAAGATTGCAAATGCACGTGAAGGAGAGTTATCACAAATGATTGATGTTGAATCG
GAACAGAAATATCAAGAAATTATTGAACTTTTAGTTACAGGTGGTTATTTTAGAGCACGT
ATTTCAGGTTTATCACCATTTGATAAAGTTGTTGGTGGCATGGCTTGGTCAATCACTGCT
TCAAATGTTGATGTTGACGCTGATTTATTCTTTCAAGAAAATTCAAATATTAAACAAAAA
GTATCATTATCAGAAGAATTAATTAAAGCATTAAATCGTATGAAATGTCCATTTCCATTA
CAAGCACAACATATTACATTATTGAATTATATAACATTATTT----------AACGTCAA
ATGGTAGAATATAAACAAAAGGTCGAACAACTCTTATCACAAGATGCCTCTACTCAAGAG
GATCTTCAAGACAGTGATAGACAACAACAAATTCAACAAGCATTCGATAGTGATATTGGT
AAATTAAAGAAATTGAAATTATTACTCAATAAAAAGAATAGAGATATCTCTTTGATTCAA
CGTTATCTCGATGAATTACCATCTCGTGCCGAACTATTACAATATCAAAGACAATTTGTT
GAATTATCCGAACAAAGTAGTTCGAAATTAACCGAAACCAGACAATACTATACAACCTAC
AATACCTTTGAAGATAAGAAAACACTTTTAGAAAATGAACTCTCAATTCTCAATTCAATT
CAATCCAAATATAGCATAGCTATGTCTTCAACAAATAATAAAGAATTATTCATTAAATCA
ATGGATCAAATCATTGATGGTATTCAACAAGTTTTAGATAAATCTGAAAATAAATGGAAT
GTTGAAAAAGTTCGTCATGATACTTTATCTGATCAATATATGACTTTATTAGATCAAGAG
AGAAATTATTATAAATTAACAAAAGACTTGAAGAAGAATGTAGAAAGAANAATTATTAAT
TAAAAAATT