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Dicty_cDB
Contig List | Clone List | cDNA Library List
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Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB529
Library VH
(Link to library)
Clone ID VHB529
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15722-1
Original site URL
Representative seq. ID VHB529P
(Link to Original site)
Representative DNA sequence 
>VHB529 (VHB529Q) /CSM/VH/VHB5-B/VHB529Q.Seq.d/
AATTTAAAAAAAATAAAGGAAAAAACACACCAAAATTAAATAAGTAAGAATAAAAATGAA
ATATTTATTAGTATATTTACACATATTACTATTTTGTTTATTAAAGATCAATAGCCAAAA
GTATTGCACTTATAGTGGTGTTGGAGAAGATTATAATATCAAATTTGATGTCTTACAAAC
ATATGGTGTTGGTTTTCATTACTCATATTTAAGTGTTATTTTTACAACTTGTAATTATAC
TATTCCTTGTGGTAGTAATAGTAATAATGTATTGTGTGGATCATATTCAATTGTTCCACG
CAAGTATCTTTCATTGGGATTTGTTAATGGCAGTACACATAGTTATAGCAAAGACGGACT
AATGATAACATATATATCACAGGATACAACAAATGATAATGATTGCAAAAGATATACAAC
AAATGTGATTTTTCAATGTAGACCAGATGGTGATGTATCCGATTCATTAGATGTTGGCAT
CGACAATATACTTATTGATTCATGTATTGTTGAATTCACACTCTCTTCACGTACAGTGTG
TCAAACATGTCCAAACTGTTTAAAAACACATCAAACATCATGTAATGTGAAAAGTGGTTG
GTGTACTTGTGATGGTAATAXXXXXXXXXXCGACNCCAAAATGGTACTGGTGATAATAAN
ACTGGTAATTGNACATGCCATAATGAACCATTTGGGTAATGGTTGTGAATTNACTCGGAT
GTCCCACTCGATNGTTCGACNCCCAAATGGGTACTTGGTGATAATAATACTTGGTAATTG
TACATGCCNATAATGAACCATTTTGGGTAATTCTTGTGAATTTACTCGATGTCCACTCGA
TTGTTCGACACCAAATGGTACTTGTGATAATAATACTGGTAATTGTACATGCCATAATGA
ACATTTTGGTAATGGTTGTGAATTCACTCAATGCCCACTCTATTGTTCAACACCAAATGG
TACTTGTGATATTAATAGTGGTATTTGTACATGTGATAATGAACATATTGGTAATGGTTG
CGAAATTAAATTTATTGAATGTAAACATAAATGTTCAACCAAACATGGAATATGTGATAA
TGATAGTGGTAATTGTAAATGTGATACTCAAACAAAAGGATTAACGTGTGAAGAATCAAG
ATTATTAATTGAATCATTAGACTCAATCAATTCAAAAGGTGGTACAATAAATATAATTGG
TTATTTTGGAAATACAACATCATTATTAACAATTAAAATTGGTGAATCACAATGTAAAAA
TATCAAAGTATTCAATGAAACAACATTAAAATGTGATATTGGTGAAGGTAAAGGTATTCA
TAATATTACAATCATTGATGATGACCTTTCGTTCACAGCAATCAATAAGTTCCAATATCT
CGATGAAAAAACAAAAATCTCAAAAATATCGAGAGGTGCAATTGCTGGTATTGTTGTTGG
TTGTGTTGTTGCTGTTTCATTAATTGGTTCTATTGCTTTTTATTATCATCACAAAAATAA
AAAGGTTAATCTTTTCTTCTAAAAACTAAAATAAAATTTAACCATTAGGGTTTTTTA
sequence update 2002.10.25
Translated Amino Acid sequence 
i*kk*rkkhtkik*VRIKMKYLLVYLHILLFCLLKINSQKYCTYSGVGEDYNIKFDVLQT
YGVGFHYSYLSVIFTTCNYTIPCGSNSNNVLCGSYSIVPRKYLSLGFVNGSTHSYSKDGL
MITYISQDTTNDNDCKRYTTNVIFQCRPDGDVSDSLDVGIDNILIDSCIVEFTLSSRTVC
QTCPNCLKTHQTSCNVKSGWCTCDGN---

---DXKMVLVIIXLVIXHAIMNHLGNGCEXTRMSHSXVRXPNGYLVIIILGNCTCX**ti
lgnsceftrcpldcstpngtcdnntgnctchnehfgngceftqcplycstpngtcdinsg
ictcdnehigngceikfieckhkcstkhgicdndsgnckcdtqtkgltceesrlliesld
sinskggtiniigyfgnttslltikigesqcknikvfnettlkcdigegkgihnitiidd
dlsftainkfqyldektkiskisrgaiagivvgcvvavsligsiafyyhhknkkvnlff*
klk*nltirvf
Translated Amino Acid sequence (All Frames) 
Frame A:
nlkkikekthqn*isknkneifisifthitilfikdq*pkvlhl*wcwrrl*yqi*cltn
iwcwfsllifkcyfynl*lyyslw*****civwiifncstqvsfigic*wqyt*l*qrrt
ndniyitgynk***lqkiynkcdfsm*trw*cirfircwhrqyty*fmyc*ihtlftysv
snmsklfkntsnim*cekwlvyl*w*---

---rxqngtgdnxtgnxtchnepfg*wl*ixsdvpldxstpkwvlgdnntw*lympimnh
fg*fl*iysmstrlfdtkwyl***yw*lymp**tfw*wl*ihsmptllfntkwyl*y**w
ylym***tyw*wlrn*iy*m*t*mfnqtwnm****w*l*m*ysnkrinv*rikiin*iir
lnqfkrwynkynwlfwkyniiinn*nw*itm*kyqsiq*nnikm*yw*r*rys*yynh**
*pfvhsnq*vpisr*knknlkniercncwyccwlccccfinwfycfllssqk*kg*sfll
ktkikfnh*gfl

Frame B:
i*kk*rkkhtkik*VRIKMKYLLVYLHILLFCLLKINSQKYCTYSGVGEDYNIKFDVLQT
YGVGFHYSYLSVIFTTCNYTIPCGSNSNNVLCGSYSIVPRKYLSLGFVNGSTHSYSKDGL
MITYISQDTTNDNDCKRYTTNVIFQCRPDGDVSDSLDVGIDNILIDSCIVEFTLSSRTVC
QTCPNCLKTHQTSCNVKSGWCTCDGN---

---DXKMVLVIIXLVIXHAIMNHLGNGCEXTRMSHSXVRXPNGYLVIIILGNCTCX**ti
lgnsceftrcpldcstpngtcdnntgnctchnehfgngceftqcplycstpngtcdinsg
ictcdnehigngceikfieckhkcstkhgicdndsgnckcdtqtkgltceesrlliesld
sinskggtiniigyfgnttslltikigesqcknikvfnettlkcdigegkgihnitiidd
dlsftainkfqyldektkiskisrgaiagivvgcvvavsligsiafyyhhknkkvnlff*
klk*nltirvf

Frame C:
fkknkgkntpklnk*e*k*niy*yiytyyyfvy*rsiaksialivvlekiiisnlmsykh
mvlvfithi*vlflqlviilflvvvivimycvdhiqlfhasifhwdllmavhiviaktd*
**hiyhriqqmimiakdiqqm*ffnvdqmvmypih*mlastiyllihvllnshslhvqcv
khvqtv*khikhhvm*kvvgvlvmvi---

---tpkwyw***xw*lxmp**tiwvmvvnxlgcptrxfdxqmgtw***ylvivhaxnepf
wvilvnlldvhsivrhqmvlviiilvivhaimnilvmvvnslnahsivqhqmvlvilivv
fvhvimnilvmvaklnllnvninvqpnmeyvimivvivnvilkqkd*rvknqdy*lnh*t
qsiqkvvq*i*lvileiqhhy*qlklvnhnvkiskysmkqh*nvilvkvkvfiilqslmm
tfrsqqsissnismkkqksqkyrevqllvlllvvlllfh*lvlllfiiitkikrlifssk
n*nki*plgff
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB529 (VHB529Q) /CSM/VH/VHB5-B/VHB529Q.Seq.d/                       2524   0.0
AHH863 (AHH863Q) /CSM/AH/AHH8-C/AHH863Q.Seq.d/                       1289   0.0
VHP265 (VHP265Q) /CSM/VH/VHP2-C/VHP265Q.Seq.d/                       1287   0.0
VHN257 (VHN257Q) /CSM/VH/VHN2-C/VHN257Q.Seq.d/                       1281   0.0
VHN234 (VHN234Q) /CSM/VH/VHN2-B/VHN234Q.Seq.d/                       1281   0.0
VHI712 (VHI712Q) /CSM/VH/VHI7-A/VHI712Q.Seq.d/                       1279   0.0
AHL231 (AHL231Q) /CSM/AH/AHL2-B/AHL231Q.Seq.d/                       1279   0.0
VHM859 (VHM859Q) /CSM/VH/VHM8-C/VHM859Q.Seq.d/                       1277   0.0
VHI141 (VHI141Q) /CSM/VH/VHI1-B/VHI141Q.Seq.d/                       1277   0.0
VHH671 (VHH671Q) /CSM/VH/VHH6-C/VHH671Q.Seq.d/                       1277   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC004153|AC004153.7 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING INPROGRESS ***, 2 unordered pieces.    38   0.010  5
AR224385|AR224385.1 Sequence 2 from patent US 6440720.             46   0.070  1
AR194032|AR194032.1 Sequence 2 from patent US 6348331.             46   0.070  1
AR162109|AR162109.1 Sequence 8 from patent US 6258559.             46   0.070  1
AG070858|AG070858.1 Pan troglodytes DNA, clone: PTB-061O01.R.      46   0.070  1
AB032076|AB032076.1 Dictyostelium discoideum mRNA for lagC2, complete cds.    36   0.082  2
AC007921|AC007921.3 Homo sapiens chromosome 18 clone RP11-391C19 map 18, WORKING DRAFT SEQUENCE, 42 unordered pieces.    42   1.1    1
AC009462|AC009462.7 Drosophila melanogaster clone BACR27G04, complete sequence.    42   1.1    1
AC007650|AC007650.7 Drosophila melanogaster, chromosome 3R, region 87F-87F, BAC clone BACR30G22, complete sequence.    42   1.1    1
AB008267|AB008267.1 Arabidopsis thaliana genomic DNA, chromosome 5, P1 clone:MMG4.    40   1.4    2
dna update 2007. 4. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117075_64(AC117075|pid:none) Dictyostelium discoideum chromoso...   125   3e-27
AC116100_9(AC116100|pid:none) Dictyostelium discoideum chromosom...   108   4e-22
AC116963_42(AC116963|pid:none) Dictyostelium discoideum chromoso...   106   2e-21
AC116920_20(AC116920|pid:none) Dictyostelium discoideum chromoso...    99   4e-19
AC116920_24(AC116920|pid:none) Dictyostelium discoideum chromoso...    98   7e-19
AC116967_7(AC116967|pid:none) Dictyostelium discoideum chromosom...    95   6e-18
AC116920_25(AC116920|pid:none) Dictyostelium discoideum chromoso...    90   2e-16
AC116967_8(AC116967|pid:none) Dictyostelium discoideum chromosom...    90   3e-16
AC116956_34(AC116956|pid:none) Dictyostelium discoideum chromoso...    89   3e-16
AC117081_39(AC117081|pid:none) Dictyostelium discoideum chromoso...    89   6e-16
protein update 2009. 6.27
PSORT 

 psg: 0.93  gvh: 0.70  alm: 0.28  top: 0.40  tms: 0.07  mit: 0.32  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.14  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 1.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 36.0 %: extracellular, including cell wall
	 32.0 %: plasma membrane
	 20.0 %: endoplasmic reticulum
	 12.0 %: Golgi

>> prediction for VHB529 is exc
5' end seq. ID VHB529F
5' end seq. 
>VHB529F.Seq
AATTTAAAAAAAATAAAGGAAAAAACACACCAAAATTAAATAAGTAAGAATAAAAATGAA
ATATTTATTAGTATATTTACACATATTACTATTTTGTTTATTAAAGATCAATAGCCAAAA
GTATTGCACTTATAGTGGTGTTGGAGAAGATTATAATATCAAATTTGATGTCTTACAAAC
ATATGGTGTTGGTTTTCATTACTCATATTTAAGTGTTATTTTTACAACTTGTAATTATAC
TATTCCTTGTGGTAGTAATAGTAATAATGTATTGTGTGGATCATATTCAATTGTTCCACG
CAAGTATCTTTCATTGGGATTTGTTAATGGCAGTACACATAGTTATAGCAAAGACGGACT
AATGATAACATATATATCACAGGATACAACAAATGATAATGATTGCAAAAGATATACAAC
AAATGTGATTTTTCAATGTAGACCAGATGGTGATGTATCCGATTCATTAGATGTTGGCAT
CGACAATATACTTATTGATTCATGTATTGTTGAATTCACACTCTCTTCACGTACAGTGTG
TCAAACATGTCCAAACTGTTTAAAAACACATCAAACATCATGTAATGTGAAAAGTGGTTG
GTGTACTTGTGATGGTAATANNNNNNNNNN
Length of 5' end seq. 630
3' end seq. ID VHB529Z
3' end seq. 
>VHB529Z.Seq
NNNNNNNNNNCGACNCCAAAATGGTACTGGTGATAATAANACTGGTAATTGNACATGCCA
TAATGAACCATTTGGGTAATGGTTGTGAATTNACTCGGATGTCCCACTCGATNGTTCGAC
NCCCAAATGGGTACTTGGTGATAATAATACTTGGTAATTGTACATGCCNATAATGAACCA
TTTTGGGTAATTCTTGTGAATTTACTCGATGTCCACTCGATTGTTCGACACCAAATGGTA
CTTGTGATAATAATACTGGTAATTGTACATGCCATAATGAACATTTTGGTAATGGTTGTG
AATTCACTCAATGCCCACTCTATTGTTCAACACCAAATGGTACTTGTGATATTAATAGTG
GTATTTGTACATGTGATAATGAACATATTGGTAATGGTTGCGAAATTAAATTTATTGAAT
GTAAACATAAATGTTCAACCAAACATGGAATATGTGATAATGATAGTGGTAATTGTAAAT
GTGATACTCAAACAAAAGGATTAACGTGTGAAGAATCAAGATTATTAATTGAATCATTAG
ACTCAATCAATTCAAAAGGTGGTACAATAAATATAATTGGTTATTTTGGAAATACAACAT
CATTATTAACAATTAAAATTGGTGAATCACAATGTAAAAATATCAAAGTATTCAATGAAA
CAACATTAAAATGTGATATTGGTGAAGGTAAAGGTATTCATAATATTACAATCATTGATG
ATGACCTTTCGTTCACAGCAATCAATAAGTTCCAATATCTCGATGAAAAAACAAAAATCT
CAAAAATATCGAGAGGTGCAATTGCTGGTATTGTTGTTGGTTGTGTTGTTGCTGTTTCAT
TAATTGGTTCTATTGCTTTTTATTATCATCACAAAAATAAAAAGGTTAATCTTTTCTTCT
AAAAACTAAAATAAAATTTAACCATTAGGGTTTTTTA
Length of 3' end seq. 937
Connected seq. ID VHB529P
Connected seq. 
>VHB529P.Seq
AATTTAAAAAAAATAAAGGAAAAAACACACCAAAATTAAATAAGTAAGAATAAAAATGAA
ATATTTATTAGTATATTTACACATATTACTATTTTGTTTATTAAAGATCAATAGCCAAAA
GTATTGCACTTATAGTGGTGTTGGAGAAGATTATAATATCAAATTTGATGTCTTACAAAC
ATATGGTGTTGGTTTTCATTACTCATATTTAAGTGTTATTTTTACAACTTGTAATTATAC
TATTCCTTGTGGTAGTAATAGTAATAATGTATTGTGTGGATCATATTCAATTGTTCCACG
CAAGTATCTTTCATTGGGATTTGTTAATGGCAGTACACATAGTTATAGCAAAGACGGACT
AATGATAACATATATATCACAGGATACAACAAATGATAATGATTGCAAAAGATATACAAC
AAATGTGATTTTTCAATGTAGACCAGATGGTGATGTATCCGATTCATTAGATGTTGGCAT
CGACAATATACTTATTGATTCATGTATTGTTGAATTCACACTCTCTTCACGTACAGTGTG
TCAAACATGTCCAAACTGTTTAAAAACACATCAAACATCATGTAATGTGAAAAGTGGTTG
GTGTACTTGTGATGGTAATA----------CGACNCCAAAATGGTACTGGTGATAATAAN
ACTGGTAATTGNACATGCCATAATGAACCATTTGGGTAATGGTTGTGAATTNACTCGGAT
GTCCCACTCGATNGTTCGACNCCCAAATGGGTACTTGGTGATAATAATACTTGGTAATTG
TACATGCCNATAATGAACCATTTTGGGTAATTCTTGTGAATTTACTCGATGTCCACTCGA
TTGTTCGACACCAAATGGTACTTGTGATAATAATACTGGTAATTGTACATGCCATAATGA
ACATTTTGGTAATGGTTGTGAATTCACTCAATGCCCACTCTATTGTTCAACACCAAATGG
TACTTGTGATATTAATAGTGGTATTTGTACATGTGATAATGAACATATTGGTAATGGTTG
CGAAATTAAATTTATTGAATGTAAACATAAATGTTCAACCAAACATGGAATATGTGATAA
TGATAGTGGTAATTGTAAATGTGATACTCAAACAAAAGGATTAACGTGTGAAGAATCAAG
ATTATTAATTGAATCATTAGACTCAATCAATTCAAAAGGTGGTACAATAAATATAATTGG
TTATTTTGGAAATACAACATCATTATTAACAATTAAAATTGGTGAATCACAATGTAAAAA
TATCAAAGTATTCAATGAAACAACATTAAAATGTGATATTGGTGAAGGTAAAGGTATTCA
TAATATTACAATCATTGATGATGACCTTTCGTTCACAGCAATCAATAAGTTCCAATATCT
CGATGAAAAAACAAAAATCTCAAAAATATCGAGAGGTGCAATTGCTGGTATTGTTGTTGG
TTGTGTTGTTGCTGTTTCATTAATTGGTTCTATTGCTTTTTATTATCATCACAAAAATAA
AAAGGTTAATCTTTTCTTCTAAAAACTAAAATAAAATTTAACCATTAGGGTTTTTTA
Length of connected seq. 1547
Full length Seq ID -
Full length Seq. -
Length of full length seq. -