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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB528
Library VH
(Link to library)
Clone ID VHB528
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16429-1
Original site URL
Representative seq. ID VHB528P
(Link to Original site)
Representative DNA sequence 
>VHB528 (VHB528Q) /CSM/VH/VHB5-B/VHB528Q.Seq.d/
AATTTTAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTTGAT
AAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAAAA
CTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAGCA
GTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAATTGG
GAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAAGAT
TTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGATTA
GGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAXXXXXXXXXXCACTGATCATATTT
CACCAGCCGGTAACATCAATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTG
TAAAACCAGAAGATTTTAACACCTATGGATCTCGTAGAGGTAATGATGAAATTATGGTTA
GAGGTACCTTTGCCAATACTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAA
CAACCTATGTACCAACTGGTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATCAAT
CAGAAGGTCATCAACTCATTGTTTTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAG
ATTGGGCTGCCAAAGGTCCATACCTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTG
AACGTATTCATAGATCCAATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAACCAG
GTCAAAATGCCTCTACTTTGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTTACCAA
CTGATAAATCCCTCATTAAAACTGGTCAAACCGTAAAGGTTACCACTAATTGTGGTAAAT
CTTTTGAAACTATCCTTCGTTTCGATACTCCAATCGAAGTTGAATATTGGGCAAATAATG
GTATCTTATCTTATGTTTTAAGAAAACTTTTACATTAATTTCTTTTTTTAATATAAAATA
AAAAAAAATAATATTATTTAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
NFNLYKLPYYLKMTTNNPFDKVKDVLKSQDQTYNFYNLSKLQDPRIEKLPYSIRILLESA
VRNCDNFEVHEKDVENILNWENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKRL
GGDPAKINPLVP---

---TDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANTRIVNKL
APAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGPYLQGIK
CVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIKTGQTVK
VTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*flfli*nkkk*yylk
Translated Amino Acid sequence (All Frames) 
Frame A:
NFNLYKLPYYLKMTTNNPFDKVKDVLKSQDQTYNFYNLSKLQDPRIEKLPYSIRILLESA
VRNCDNFEVHEKDVENILNWENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKRL
GGDPAKINPLVP---

---h*syftsr*hq*kiisrslfrvqrcktrrf*hlwis*r***nyg*rylcqysyc**i
gssrwstnnlctnw*vnvyfrccrkisirrssthcfgw**lwlw*f*rlgcqrsipsrn*
vchchli*tys*iqsrwygyypiaiptrskclyfgshw*rtiqyrvtn**iph*nwsnrk
gyh*lw*if*nypsfrysnrs*ilgk*wylilcfkktftlisffnik*kkiilfk

Frame B:
iliyinylit*r*qqiihlik*rmy*nhkikliifiifqnfktqelknyhtpleffwnqq
fvivitlkytkkmlkis*igktlqiklkflsnqqeyyykillvyqqllilqq*emp*kd*
vviqqklthlf---

---TDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANTRIVNKL
APAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGPYLQGIK
CVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIKTGQTVK
VTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*flfli*nkkk*yylk

Frame C:
f*fi*itlllkddnk*si**skgcikitrsnl*fl*sfktsrpkn*kitilh*nsfgiss
s*l**l*strkrc*kylklgkhck*s*nsfqtsksiitrfywctssc*scsnerchekir
w*sskn*ptcs---

---liifhqpvtsienhqplvi*spkv*nqkiltpmdlvevmmklwlevplpilvllinw
lqplvhkqpmyqlvs*clfqmlqkninqkvinslfwlvvimalvvleiglpkvhtfkels
vslpshlnvfidpislvwvlshcnsnqvkmpllwvslvknnsissyqlinpslklvkp*r
lplivvnllklsfvsilqsklnigqimvsylmf*enfyinfff*ykikknnii*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB528 (VHB528Q) /CSM/VH/VHB5-B/VHB528Q.Seq.d/                       2079   0.0
VHQ461 (VHQ461Q) /CSM/VH/VHQ4-C/VHQ461Q.Seq.d/                       1334   0.0
VHQ432 (VHQ432Q) /CSM/VH/VHQ4-B/VHQ432Q.Seq.d/                       1334   0.0
VHQ267 (VHQ267Q) /CSM/VH/VHQ2-C/VHQ267Q.Seq.d/                       1334   0.0
VHQ262 (VHQ262Q) /CSM/VH/VHQ2-C/VHQ262Q.Seq.d/                       1334   0.0
VHP311 (VHP311Q) /CSM/VH/VHP3-A/VHP311Q.Seq.d/                       1334   0.0
VHO844 (VHO844Q) /CSM/VH/VHO8-B/VHO844Q.Seq.d/                       1334   0.0
VHO678 (VHO678Q) /CSM/VH/VHO6-D/VHO678Q.Seq.d/                       1334   0.0
VHK750 (VHK750Q) /CSM/VH/VHK7-C/VHK750Q.Seq.d/                       1334   0.0
VHK111 (VHK111Q) /CSM/VH/VHK1-A/VHK111Q.Seq.d/                       1334   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC007170|AC007170.6 Arabidopsis thaliana chromosome 2 clone T3P4 map mi310, complete sequence.    42   0.011  3
AE006300|AE006300.1 Lactococcus lactis subsp. lactis IL1403 section 62 of 218 of the complete genome.    42   0.13   2
AB209403|AB209403.1 Homo sapiens mRNA for iron-responsive element binding protein 2 variant protein.    32   0.66   3
AC007858|AC007858.1 Oryza sativa (japonica cultivar-group) chromosome 5 clone BAC 10A19I, complete sequence.    42   0.78   1
AC074309|AC074309.4 Arabidopsis thaliana chromosome 1 BAC T12O21 genomic sequence, complete sequence.    36   2.6    2
AB084152|AB084152.1 Clostridium botulinum bontb gene for neurotoxin, complete cds.    36   2.6    2
AC005312|AC005312.3 Arabidopsis thaliana chromosome 2 clone T16F16 map CIC11A04, complete sequence.    40   3.1    1
AC002340|AC002340.3 Arabidopsis thaliana chromosome 2 BAC T11J7 genomic sequence, complete sequence.    40   3.1    1
AC010579|AC010579.9 Drosophila melanogaster, chromosome 3R, region 96F-96F, BAC clone BACR09D08, complete sequence.    40   3.1    1
AC146966|AC146966.3 Bos taurus clone RP42-139G14, complete sequence.    40   3.1    1
dna update 2007. 4. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54X73) RecName: Full=Probable cytoplasmic aconitate hydratase;...   436   e-121
AB236788_1(AB236788|pid:none) Trifolium pratense RNA for putativ...   284   3e-75
AP008209_256(AP008209|pid:none) Oryza sativa (japonica cultivar-...   283   1e-74
BT060820_1(BT060820|pid:none) Zea mays full-length cDNA clone ZM...   281   4e-74
(Q23500) RecName: Full=Probable cytoplasmic aconitate hydratase;...   280   5e-74
BT060665_1(BT060665|pid:none) Zea mays full-length cDNA clone ZM...   280   6e-74
(P49608) RecName: Full=Aconitate hydratase, cytoplasmic;        ...   277   5e-73
DQ294230_1(DQ294230|pid:none) Glossina morsitans morsitans TC165...   276   7e-73
(Q9SIB9) RecName: Full=Aconitate hydratase 2, mitochondrial;    ...   276   1e-72
AY050485_1(AY050485|pid:none) Prunus avium putative aconitase mR...   275   3e-72
protein update 2009. 6.27
PSORT 

 psg: 0.75  gvh: 0.20  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall

>> prediction for VHB528 is nuc
5' end seq. ID VHB528F
5' end seq. 
>VHB528F.Seq
AATTTTAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTTGAT
AAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAAAA
CTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAGCA
GTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAATTGG
GAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAAGAT
TTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGATTA
GGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCANNNNNNNNNN
Length of 5' end seq. 406
3' end seq. ID VHB528Z
3' end seq. 
>VHB528Z.Seq
NNNNNNNNNNCACTGATCATATTTCACCAGCCGGTAACATCAATAGAAAATCATCAGCCG
CTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACACCTATGGATCTCGTA
GAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCGTATTGTTAATAAAT
TGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGAGTTAATGTTTATTT
CAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGTTTTGGCTGGTAGTG
ATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATACCTTCAAGGAATTA
AGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATCCAATCTCGTTGGTATGGGTA
TTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGGTCTCACTGGTAAAG
AACAATTCAATATCGAGTTACCAACTGATAAATCCCTCATTAAAACTGGTCAAACCGTAA
AGGTTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTTCGATACTCCAATCG
AAGTTGAATATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAGAAAACTTTTACATT
AATTTCTTTTTTTAATATAAAATAAAAAAAAATAATATTATTTAAAA
Length of 3' end seq. 707
Connected seq. ID VHB528P
Connected seq. 
>VHB528P.Seq
AATTTTAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTTGAT
AAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAAAA
CTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAGCA
GTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAATTGG
GAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAAGAT
TTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGATTA
GGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCA----------CACTGATCATATTT
CACCAGCCGGTAACATCAATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTG
TAAAACCAGAAGATTTTAACACCTATGGATCTCGTAGAGGTAATGATGAAATTATGGTTA
GAGGTACCTTTGCCAATACTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAA
CAACCTATGTACCAACTGGTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATCAAT
CAGAAGGTCATCAACTCATTGTTTTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAG
ATTGGGCTGCCAAAGGTCCATACCTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTG
AACGTATTCATAGATCCAATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAACCAG
GTCAAAATGCCTCTACTTTGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTTACCAA
CTGATAAATCCCTCATTAAAACTGGTCAAACCGTAAAGGTTACCACTAATTGTGGTAAAT
CTTTTGAAACTATCCTTCGTTTCGATACTCCAATCGAAGTTGAATATTGGGCAAATAATG
GTATCTTATCTTATGTTTTAAGAAAACTTTTACATTAATTTCTTTTTTTAATATAAAATA
AAAAAAAATAATATTATTTAAAA
Length of connected seq. 1093
Full length Seq ID -
Full length Seq. -
Length of full length seq. -