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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB485
Library VH
(Link to library)
Clone ID VHB485
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11181-1
Original site URL
Representative seq. ID VHB485P
(Link to Original site)
Representative DNA sequence 
>VHB485 (VHB485Q) /CSM/VH/VHB4-D/VHB485Q.Seq.d/
AATTTTTTTAATTTTTTTTCAAAAAGAGACATAAAAGATGGAAAGTTTAAGTGAACAAGA
AATAAAGTATAGAGAAGATACAACTAAAGTTTACAGGGAGTTATTATCAATTCCAACAGT
CAACAACGCATTTTTTACAAACAATGATGAAAGTAATGAAAATCAAACATCAGCATCAAT
CATTGTATCTCAAATTGATATCACCAATAAGAAAACTAAATTTTATATGTCACAAAAAAC
AATTTTAAAAGATTCATCATCATCATCAAAGAAAAACCAAGTAATTTCATCAGCAATTCA
ACAAGAATTAGCAACTTCAATCATATCAGTATCACCATCACATAAGAAAGTTTTAACAAT
TAAAGAGAACCCTTCAACTGAATTCGAATTCTCTTTTGATATTACTGATGGTTCACATTT
AATTACTTCAATTACTTCAAAAGATATTCATAAAAAAATTTTAAATGATGAATGGTTTGG
TAGTTTTTCATGGTCACCATGTGAGAAATATGTAGCATTTATTGCAGATTCAAAATTAAA
TGTAACAGGATTTTTTGATAAGGAACCAAAAGATAAAGCTATTGGTGATCAATTTGTATT
TAGAGATGATTGGGGTGAAACTTXXXXXXXXXXCCATTGGGTTGTTTAGTTTTCATTCAT
GGTGGTCCACATTCAAATTTAGATGTTGGTTATGTTTCAACAATCACTTATTTACTCTCA
TTGGGTTATAATATTATAATTCCAAATTATAGAGGTTCAACAGGTTATGGTAAAGATTTC
GTAGATTGTTTACCAGGTTATATTGGTGATTTGGATGTTGACGATTGTGTTCAAGCAATT
AATTACACACTCGATAAAATCGATAATACAATCGATAGAAATAAAATTGGTGTTATTGGT
GGTAGTCATGGTGGTTTCTTATCAGCACATCTAAGTAGATTCCCAATTGTCAAAACTTCA
ATCATGAGAAATCCTGTAATCGATATTCCATCAATGTCAACCTTATCCGATATTCCAGAT
TGGTGTTTCTTTGAAGCTGGTATTAATCTCTCTGATCCAACCTCTCAATATCATACATTA
CCATCAATGGAAGAAATTGAAAAAATGAGAAAATGTTCACCAAGTTTCCATATTGAAAAC
ATCAAAATTCCATCATTATTAGCTTTAGGTGATTCAGATCTTCGTGTTCCACCTTCTCAA
GGTTTATTATATTATCGTATGTTAAGAGAAAGAGATGTACCAACAAAATGTTTAATGTAT
CCAAAAACTGGTCATAGTCTTGATTCAATTGATGCTCGTTTAGATCAATGGGTTAATATT
TCATTATGGTTAAAAAAATATTTAAATTAAAATTTTTAAAAAAAATTACAACAATTACAC
CACCACCACCACCACTACCACCACCATAGGTAAAATAATATTATTAAATAAAAACATTT
sequence update 2002.10.25
Translated Amino Acid sequence 
ifliffqket*KMESLSEQEIKYREDTTKVYRELLSIPTVNNAFFTNNDESNENQTSASI
IVSQIDITNKKTKFYMSQKTILKDSSSSSKKNQVISSAIQQELATSIISVSPSHKKVLTI
KENPSTEFEFSFDITDGSHLITSITSKDIHKKILNDEWFGSFSWSPCEKYVAFIADSKLN
VTGFFDKEPKDKAIGDQFVFRDDWGET---

---PLGCLVFIHGGPHSNLDVGYVSTITYLLSLGYNIIIPNYRGSTGYGKDFVDCLPGYI
GDLDVDDCVQAINYTLDKIDNTIDRNKIGVIGGSHGGFLSAHLSRFPIVKTSIMRNPVID
IPSMSTLSDIPDWCFFEAGINLSDPTSQYHTLPSMEEIEKMRKCSPSFHIENIKIPSLLA
LGDSDLRVPPSQGLLYYRMLRERDVPTKCLMYPKTGHSLDSIDARLDQWVNISLWLKKYL
N*nf*kklqqlhhhhhhyhhhr*nniik*kh
Translated Amino Acid sequence (All Frames) 
Frame A:
nffnffskrdikdgkfk*trnkv*rryn*slqgviinsnsqqrifykq**k**ksnisin
hcisn*yhq*en*ilyvtknnfkrfiiiikekpsnfisnstrisnfnhisitit*esfnn
*repfn*irilf*yy*wftfnyfnyfkrys*knfk**mvw*ffmvtm*eicsiycrfkik
cnrif**gtkr*syw*sici*r*lg*n---

---PLGCLVFIHGGPHSNLDVGYVSTITYLLSLGYNIIIPNYRGSTGYGKDFVDCLPGYI
GDLDVDDCVQAINYTLDKIDNTIDRNKIGVIGGSHGGFLSAHLSRFPIVKTSIMRNPVID
IPSMSTLSDIPDWCFFEAGINLSDPTSQYHTLPSMEEIEKMRKCSPSFHIENIKIPSLLA
LGDSDLRVPPSQGLLYYRMLRERDVPTKCLMYPKTGHSLDSIDARLDQWVNISLWLKKYL
N*nf*kklqqlhhhhhhyhhhr*nniik*kh

Frame B:
ifliffqket*KMESLSEQEIKYREDTTKVYRELLSIPTVNNAFFTNNDESNENQTSASI
IVSQIDITNKKTKFYMSQKTILKDSSSSSKKNQVISSAIQQELATSIISVSPSHKKVLTI
KENPSTEFEFSFDITDGSHLITSITSKDIHKKILNDEWFGSFSWSPCEKYVAFIADSKLN
VTGFFDKEPKDKAIGDQFVFRDDWGET---

---hwvv*fsfmvvhiqi*mlvmfqqsliyshwviil*fqiievqqvmvkis*ivyqvil
viwmltivfkqlithsiksiiqsieiklvllvvvmvvsyqhi*vdsqlsklqs*eil*si
fhqcqpypifqigvslklvlisliqplniihyhqwkklkk*envhqvsilktskfhhy*l
*viqifvfhllkvyyiivc*ekemyqqnv*ciqklvivliqlmlv*inglifhyg*kni*
ikifkknynnytttttttttigkiillnkni

Frame C:
ff*fffkkrhkrwkv*vnkk*siekiqlkftgsyyqfqqstthflqtmmkvmkikhqhqs
lylklispirklnfichkkqf*kihhhhqrktk*fhqqfnkn*qlqsyqyhhhirkf*ql
krtlqlnsnsllillmvhi*llqllqkifikkf*mmnglvvfhghhvrnm*hllqiqn*m
*qdflirnqkikllvinlylemigvkl---

---iglfsfhswwstfkfrcwlcfnnhlftligl*yynskl*rfnrlw*rfrrlftrlyw
*fgc*rlcssn*lhtr*nr*ynr*k*nwcyww*swwflistsk*ipncqnfnhekscnry
sinvnlirysrlvfl*swy*sl*snlsisyitingrn*knekmftkfpy*khqnsiiisf
r*frsscstfsrfiilsyvkrkrctnkmfnvsknws*s*fn*csfrsmg*yfimvkkifk
lkflkkittitppppplppp*vk*yy*iktf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB485 (VHB485Q) /CSM/VH/VHB4-D/VHB485Q.Seq.d/                       2498   0.0
VHO522 (VHO522Q) /CSM/VH/VHO5-A/VHO522Q.Seq.d/                       1374   0.0
VHO634 (VHO634Q) /CSM/VH/VHO6-B/VHO634Q.Seq.d/                       1366   0.0
VHP838 (VHP838Q) /CSM/VH/VHP8-B/VHP838Q.Seq.d/                       1358   0.0
AHD261 (AHD261Q) /CSM/AH/AHD2-C/AHD261Q.Seq.d/                       1342   0.0
CHI527 (CHI527Q) /CSM/CH/CHI5-B/CHI527Q.Seq.d/                       1336   0.0
VHB191 (VHB191Q) /CSM/VH/VHB1-D/VHB191Q.Seq.d/                       1306   0.0
VHI656 (VHI656Q) /CSM/VH/VHI6-C/VHI656Q.Seq.d/                       1298   0.0
CHQ849 (CHQ849Q) /CSM/CH/CHQ8-C/CHQ849Q.Seq.d/                       1296   0.0
AHQ746 (AHQ746Q) /CSM/AH/AHQ7-B/AHQ746Q.Seq.d/                       1292   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC004708|AC004708.1 CIC5B11.1  check: 4870  from: 1  to: 167234, complete sequence.    46   0.065  1
AC145774|AC145774.1 Pan troglodytes BAC clone RP43-160F1 from chromosome 7, complete sequence.    42   0.22   2
AB020742|AB020742.1 Arabidopsis thaliana genomic DNA, chromosome 5, TAC clone:K21H1.    38   0.24   3
AC068909|AC068909.31 Mus musculus BAC Clone 407f20 RPCI-23, complete sequence.    44   0.26   1
AB019229|AB019229.1 Arabidopsis thaliana genomic DNA, chromosome 3, P1 clone: MDC16.    38   0.37   2
AC005850|AC005850.1 Arabidopsis thaliana chromosome I BAC T25B24 genomic sequence, complete sequence.    42   0.38   4
AC145918|AC145918.3 Pan troglodytes BAC clone RP43-9J6 from chromosome 7, complete sequence.    40   0.55   5
AC004136|AC004136.3 Arabidopsis thaliana chromosome 2 BAC T8K22 genomic sequence, complete sequence.    38   0.55   4
AJ007302|AJ007302.1 Sus scrofa triadin gene.                       34   0.70   3
AE006266|AE006266.1 Lactococcus lactis subsp. lactis IL1403 section 28 of 218 of the complete genome.    42   1.0    1
dna update 2007. 4. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P13676) RecName: Full=Acylamino-acid-releasing enzyme;         ...   174   5e-42
BC034199_1(BC034199|pid:none) Mus musculus acylpeptide hydrolase...   172   2e-41
BC026594_1(BC026594|pid:none) Mus musculus acylpeptide hydrolase...   172   2e-41
EU972122_1(EU972122|pid:none) Zea mays clone 375508 acylamino-ac...   172   2e-41
(Q8R146) RecName: Full=Acylamino-acid-releasing enzyme;         ...   172   2e-41
(P19205) RecName: Full=Acylamino-acid-releasing enzyme;         ...   171   8e-41
D38441_1(D38441|pid:none) Human mRNA for acylamino acid-releasin...   169   2e-40
BC057485_1(BC057485|pid:none) Danio rerio acylpeptide hydrolase,...   168   5e-40
(P80227) RecName: Full=Acylamino-acid-releasing enzyme;         ...   167   7e-40
CP001333_256(CP001333|pid:none) Micromonas sp. RCC299 chromosome...   162   3e-38
protein update 2009. 6.27
PSORT 

 psg: 0.75  gvh: 0.26  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHB485 is cyt
5' end seq. ID VHB485F
5' end seq. 
>VHB485F.Seq
AATTTTTTTAATTTTTTTTCAAAAAGAGACATAAAAGATGGAAAGTTTAAGTGAACAAGA
AATAAAGTATAGAGAAGATACAACTAAAGTTTACAGGGAGTTATTATCAATTCCAACAGT
CAACAACGCATTTTTTACAAACAATGATGAAAGTAATGAAAATCAAACATCAGCATCAAT
CATTGTATCTCAAATTGATATCACCAATAAGAAAACTAAATTTTATATGTCACAAAAAAC
AATTTTAAAAGATTCATCATCATCATCAAAGAAAAACCAAGTAATTTCATCAGCAATTCA
ACAAGAATTAGCAACTTCAATCATATCAGTATCACCATCACATAAGAAAGTTTTAACAAT
TAAAGAGAACCCTTCAACTGAATTCGAATTCTCTTTTGATATTACTGATGGTTCACATTT
AATTACTTCAATTACTTCAAAAGATATTCATAAAAAAATTTTAAATGATGAATGGTTTGG
TAGTTTTTCATGGTCACCATGTGAGAAATATGTAGCATTTATTGCAGATTCAAAATTAAA
TGTAACAGGATTTTTTGATAAGGAACCAAAAGATAAAGCTATTGGTGATCAATTTGTATT
TAGAGATGATTGGGGTGAAACTTNNNNNNNNNN
Length of 5' end seq. 633
3' end seq. ID VHB485Z
3' end seq. 
>VHB485Z.Seq
NNNNNNNNNNCCATTGGGTTGTTTAGTTTTCATTCATGGTGGTCCACATTCAAATTTAGA
TGTTGGTTATGTTTCAACAATCACTTATTTACTCTCATTGGGTTATAATATTATAATTCC
AAATTATAGAGGTTCAACAGGTTATGGTAAAGATTTCGTAGATTGTTTACCAGGTTATAT
TGGTGATTTGGATGTTGACGATTGTGTTCAAGCAATTAATTACACACTCGATAAAATCGA
TAATACAATCGATAGAAATAAAATTGGTGTTATTGGTGGTAGTCATGGTGGTTTCTTATC
AGCACATCTAAGTAGATTCCCAATTGTCAAAACTTCAATCATGAGAAATCCTGTAATCGA
TATTCCATCAATGTCAACCTTATCCGATATTCCAGATTGGTGTTTCTTTGAAGCTGGTAT
TAATCTCTCTGATCCAACCTCTCAATATCATACATTACCATCAATGGAAGAAATTGAAAA
AATGAGAAAATGTTCACCAAGTTTCCATATTGAAAACATCAAAATTCCATCATTATTAGC
TTTAGGTGATTCAGATCTTCGTGTTCCACCTTCTCAAGGTTTATTATATTATCGTATGTT
AAGAGAAAGAGATGTACCAACAAAATGTTTAATGTATCCAAAAACTGGTCATAGTCTTGA
TTCAATTGATGCTCGTTTAGATCAATGGGTTAATATTTCATTATGGTTAAAAAAATATTT
AAATTAAAATTTTTAAAAAAAATTACAACAATTACACCACCACCACCACCACTACCACCA
CCATAGGTAAAATAATATTATTAAATAAAAACATTT
Length of 3' end seq. 816
Connected seq. ID VHB485P
Connected seq. 
>VHB485P.Seq
AATTTTTTTAATTTTTTTTCAAAAAGAGACATAAAAGATGGAAAGTTTAAGTGAACAAGA
AATAAAGTATAGAGAAGATACAACTAAAGTTTACAGGGAGTTATTATCAATTCCAACAGT
CAACAACGCATTTTTTACAAACAATGATGAAAGTAATGAAAATCAAACATCAGCATCAAT
CATTGTATCTCAAATTGATATCACCAATAAGAAAACTAAATTTTATATGTCACAAAAAAC
AATTTTAAAAGATTCATCATCATCATCAAAGAAAAACCAAGTAATTTCATCAGCAATTCA
ACAAGAATTAGCAACTTCAATCATATCAGTATCACCATCACATAAGAAAGTTTTAACAAT
TAAAGAGAACCCTTCAACTGAATTCGAATTCTCTTTTGATATTACTGATGGTTCACATTT
AATTACTTCAATTACTTCAAAAGATATTCATAAAAAAATTTTAAATGATGAATGGTTTGG
TAGTTTTTCATGGTCACCATGTGAGAAATATGTAGCATTTATTGCAGATTCAAAATTAAA
TGTAACAGGATTTTTTGATAAGGAACCAAAAGATAAAGCTATTGGTGATCAATTTGTATT
TAGAGATGATTGGGGTGAAACTT----------CCATTGGGTTGTTTAGTTTTCATTCAT
GGTGGTCCACATTCAAATTTAGATGTTGGTTATGTTTCAACAATCACTTATTTACTCTCA
TTGGGTTATAATATTATAATTCCAAATTATAGAGGTTCAACAGGTTATGGTAAAGATTTC
GTAGATTGTTTACCAGGTTATATTGGTGATTTGGATGTTGACGATTGTGTTCAAGCAATT
AATTACACACTCGATAAAATCGATAATACAATCGATAGAAATAAAATTGGTGTTATTGGT
GGTAGTCATGGTGGTTTCTTATCAGCACATCTAAGTAGATTCCCAATTGTCAAAACTTCA
ATCATGAGAAATCCTGTAATCGATATTCCATCAATGTCAACCTTATCCGATATTCCAGAT
TGGTGTTTCTTTGAAGCTGGTATTAATCTCTCTGATCCAACCTCTCAATATCATACATTA
CCATCAATGGAAGAAATTGAAAAAATGAGAAAATGTTCACCAAGTTTCCATATTGAAAAC
ATCAAAATTCCATCATTATTAGCTTTAGGTGATTCAGATCTTCGTGTTCCACCTTCTCAA
GGTTTATTATATTATCGTATGTTAAGAGAAAGAGATGTACCAACAAAATGTTTAATGTAT
CCAAAAACTGGTCATAGTCTTGATTCAATTGATGCTCGTTTAGATCAATGGGTTAATATT
TCATTATGGTTAAAAAAATATTTAAATTAAAATTTTTAAAAAAAATTACAACAATTACAC
CACCACCACCACCACTACCACCACCATAGGTAAAATAATATTATTAAATAAAAACATTT
Length of connected seq. 1429
Full length Seq ID -
Full length Seq. -
Length of full length seq. -