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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB482
Library VH
(Link to library)
Clone ID VHB482
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHB482P
(Link to Original site)
Representative DNA sequence 
>VHB482 (VHB482Q) /CSM/VH/VHB4-D/VHB482Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
XXXXXXXXXXCGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAG
GTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTA
AAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAA
ACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACA
NAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTG
CCTCCCCAACTNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTA
TTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTC
GTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTT
TCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATC
ATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCA
CCAGAAANAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAAC
TCTAAACAATTTAAGTCGCACTTGAGTTTTCAAACATAGNAATCAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGN---

---EWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDEN
MRGIRFNLYDVTLHTDAIHXGGGQIIPTARRVLYAAELTASPTXLEPXYLVEITAPENAI
GGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDH
WASIGVVNKDKKATEVALATRXRKGLAPEIPDLDKFHEKL*ti*valefsnixik
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvt---

---rmg*k*chehlvfrsrr*rcqsprqchqrcsife*n*rffrwcfpmghqrrccl**k
herypfqsl*chpph*chpxrwwsnhpncssctlcc*inclpnxxrtnxlsrnycprkry
wwyllctqss*rycyw*rtsyrfpiilcqssftsirilrfhr*ftfphcrssfptmclrs
lgfnwccqqr*esh*srfshqkxkrfsprnprs*qiprktlnnlsrt*vfkhxnq

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*---

---EWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDEN
MRGIRFNLYDVTLHTDAIHXGGGQIIPTARRVLYAAELTASPTXLEPXYLVEITAPENAI
GGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDH
WASIGVVNKDKKATEVALATRXRKGLAPEIPDLDKFHEKL*ti*valefsnixik

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGN---

---ngikmmp*tfglsvqkvtvpisssmspkvfni*mklkilslvlsngppkkvlfvmkt
*evsvsifmmspstlmpstxvvvkssqllvvysmlln*lppqlx*nqxt**kllpqktll
vvftlysivvevlllvknvvsvphyslskliyqy*npsvspliyvptlpvklshnvssii
glqlvlstkirkplksl*ppexekv*pqksqiltnstknskqfkshlsfqt*xsk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB482 (VHB482Q) /CSM/VH/VHB4-D/VHB482Q.Seq.d/                       2458   0.0
VHQ186 (VHQ186Q) /CSM/VH/VHQ1-D/VHQ186Q.Seq.d/                       1340   0.0
VHP870 (VHP870Q) /CSM/VH/VHP8-C/VHP870Q.Seq.d/                       1340   0.0
VHJ291 (VHJ291Q) /CSM/VH/VHJ2-D/VHJ291Q.Seq.d/                       1340   0.0
VHE421 (VHE421Q) /CSM/VH/VHE4-A/VHE421Q.Seq.d/                       1340   0.0
VHD209 (VHD209Q) /CSM/VH/VHD2-A/VHD209Q.Seq.d/                       1340   0.0
VHD127 (VHD127Q) /CSM/VH/VHD1-B/VHD127Q.Seq.d/                       1340   0.0
VHD104 (VHD104Q) /CSM/VH/VHD1-A/VHD104Q.Seq.d/                       1340   0.0
VHC525 (VHC525Q) /CSM/VH/VHC5-B/VHC525Q.Seq.d/                       1340   0.0
VHB840 (VHB840Q) /CSM/VH/VHB8-B/VHB840Q.Seq.d/                       1340   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

EF059386|EF059386.1 Synthetic construct Saccharomyces cerevisiae clone FLH202901.01X EFT2, complete sequence.    62   2e-34  7
AR223730|AR223730.1 Sequence 3 from patent US 6440409.             62   1e-33  7
AR223729|AR223729.1 Sequence 1 from patent US 6440409.             62   2e-33  7
AB023579|AB023579.1 Plasmodium falciparum gene for elongation factor 2, partial cds.    76   1e-21  5
AC009894|AC009894.2 Arabidopsis thaliana chromosome I BAC T6H22 genomic sequence, complete sequence.    60   4e-16  5
AB095183|AB095183.1 Cyanidioschyzon merolae gene for elongation factor-2, complete cds.    40   0.013  2
AC009254|AC009254.8 Drosophila melanogaster, chromosome 2L, region 40A-40C, BAC clone BACR42E05, complete sequence.    46   0.059  1
AB190260|AB190260.1 Caenorhabditis elegans gmn-1 mRNA for geminin homolog, complete cds.    32   0.15   2
AC010823|AC010823.4 Homo sapiens clone RP11-4P4, WORKING DRAFT SEQUENCE, 4 unordered pieces.    44   0.23   1
AC091272|AC091272.12 Mus musculus chromosome 1, clone RP23-74B7, complete sequence.    38   0.78   2
dna update 2007. 4. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB171046_1(AB171046|pid:none) Macaca fascicularis brain cDNA clo...   387   e-106
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   277   7e-73
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   276   2e-72
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   275   3e-72
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   275   3e-72
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   274   6e-72
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   274   6e-72
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   273   8e-72
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   271   3e-71
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   271   3e-71
protein update 2009. 6.27
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall

>> prediction for VHB482 is nuc
5' end seq. ID VHB482F
5' end seq. 
>VHB482F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
NNNNNNNNNN
Length of 5' end seq. 610
3' end seq. ID VHB482Z
3' end seq. 
>VHB482Z.Seq
NNNNNNNNNNCGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAG
GTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTA
AAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAA
ACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACA
NAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTG
CCTCCCCAACTNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTA
TTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTC
GTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTT
TCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATC
ATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCA
CCAGAAANAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAAC
TCTAAACAATTTAAGTCGCACTTGAGTTTTCAAACATAGNAATCAAAA
Length of 3' end seq. 708
Connected seq. ID VHB482P
Connected seq. 
>VHB482P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
----------CGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAG
GTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTA
AAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAA
ACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACA
NAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTG
CCTCCCCAACTNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTA
TTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTC
GTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTT
TCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATC
ATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCA
CCAGAAANAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAAC
TCTAAACAATTTAAGTCGCACTTGAGTTTTCAAACATAGNAATCAAAA
Length of connected seq. 1298
Full length Seq ID -
Full length Seq. -
Length of full length seq. -