>VHB478 (VHB478Q) /CSM/VH/VHB4-D/VHB478Q.Seq.d/
XXXXXXXXXXTGCGCATCAGGTTCAGTTGTTGANCAATGTTCATCAGTCGATTCAATTAG
TAATTTACCAACCNCAATGCAATTATTCGCTGGTATTAAAAGTATTTGCACAGAGATGGC
AATGGATGGTTGTGAGAAATGTTCAGGAAATTCACCAACTACCACCTGTGATGTTTTGCC
AGTTTACTCCTCCCTTTGTATGGCTATGCCTGATATGTCACAATGTGCCAATTGGACCAA
GATGTGTTCCTCCTCTGGTCAACTCTACAACTCTCAAATCACCACAGACTATTGTGTCGC
CTCTGTTGCTGACGCCGTTCCAATCATGAGAATGTACTTTCACACTGGTATCTTGGACTA
TATCCTCTTTAAATCTTGGGTACCAAGAACTGACCGTCAATTTGCTGGTTCATGGTNTGC
CATNNTNTTNTGTGCNATCTTTTTTGAACTCGAAAAAACCCTTAGAAGTATTTTAGAAAA
AAGATGGACTCCCAACAAAAAAGATTCANAAGATAATANTTTAATTAAGTTCATCTTTCC
TTAAGTGGTTTNTATC

---ASGSVVXQCSSVDSISNLPTXMQLFAGIKSICTEMAMDGCEKCSGNSPTTTCDVLPV
YSSLCMAMPDMSQCANWTKMCSSSGQLYNSQITTDYCVASVADAVPIMRMYFHTGILDYI
LFKSWVPRTDRQFAGSWXAXXXCAIFFELEKTLRSILEKRWTPNKKDSXDNXLIKFIFP*
vvxi

Frame A:
---ahqvqllxnvhqsiqlviyqpqcnyslvlkvfaqrwqwmvvrnvqeihqlppvmfcq
ftppfvwlclichnvpigprcvpplvnsttlkspqtivspllltpfqs*ectftlvswti
sslnlgyqeltvnllvhgxpxxxvxsflnskkplevf*kkdglptkkixkiix*lsssfl
kwfx


Frame B:
---rirfsc*xmfisrfn**ftnxnaiirwy*kylhrdgngwl*emfrkftnyhl*cfas
lllplygya*yvtmcqldqdvfllwstlqlsnhhrllcrlcc*rrsnhenvlshwylgly
pl*ilgtkn*psicwfmvchxxxcxlf*trknp*kyfrkkmdsqqkrfxr*xfn*vhlsl
sgxy


Frame C:
---ASGSVVXQCSSVDSISNLPTXMQLFAGIKSICTEMAMDGCEKCSGNSPTTTCDVLPV
YSSLCMAMPDMSQCANWTKMCSSSGQLYNSQITTDYCVASVADAVPIMRMYFHTGILDYI
LFKSWVPRTDRQFAGSWXAXXXCAIFFELEKTLRSILEKRWTPNKKDSXDNXLIKFIFP*
vvxi

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB478 (VHB478Q) /CSM/VH/VHB4-D/VHB478Q.Seq.d/                       1017   0.0
VFF667 (VFF667Q) /CSM/VF/VFF6-C/VFF667Q.Seq.d/                        944   0.0
SFH236 (SFH236Q) /CSM/SF/SFH2-B/SFH236Q.Seq.d/                        942   0.0
CFH242 (CFH242Q) /CSM/CF/CFH2-B/CFH242Q.Seq.d/                        940   0.0
VFE385 (VFE385Q) /CSM/VF/VFE3-D/VFE385Q.Seq.d/                        938   0.0
VHM772 (VHM772Q) /CSM/VH/VHM7-C/VHM772Q.Seq.d/                        936   0.0
VHE424 (VHE424Q) /CSM/VH/VHE4-A/VHE424Q.Seq.d/                        936   0.0
VFD235 (VFD235Q) /CSM/VF/VFD2-B/VFD235Q.Seq.d/                        936   0.0
VFC744 (VFC744Q) /CSM/VF/VFC7-B/VFC744Q.Seq.d/                        928   0.0
VFJ390 (VFJ390Q) /CSM/VF/VFJ3-D/VFJ390Q.Seq.d/                        918   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC007770|AC007770.5 Drosophila melanogaster, chromosome 3R, region 86C-86C, BAC clone BACR03J13, complete sequence.    42   0.38   1
AC007331|AC007331.1 Homo sapiens chromosome 4, *** SEQUENCING IN PROGRESS ***, 32 unordered pieces.    38   6.0    1
AC005718|AC005718.10 Drosophila melanogaster strain y; cn bw sp chromosome 2 clone DS02336 (D440) map 60C8-60D2, *** SEQUENCING IN PROGRESS ***, 68 unordered pieces.    38   6.0    1
AG081185|AG081185.1 Pan troglodytes DNA, clone: PTB-077J05.F.      38   6.0    1
AC025781|AC025781.8 Arabidopsis thaliana chromosome 1 BAC F15C21 genomic sequence, complete sequence.    38   6.0    1
AC008353|AC008353.4 Drosophila melanogaster, chromosome 2R, region 60C-60C, BAC clone BACR02A05, complete sequence.    38   6.0    1
AC008325|AC008325.5 Drosophila melanogaster clone BACR05M06, complete sequence.    38   6.0    1
AC008324|AC008324.3 Drosophila melanogaster clone BACR25K01, complete sequence.    38   6.0    1
AC083909|AC083909.20 Mus musculus 1 BAC RP23-424A2 (Roswell Park Cancer Institute Mouse BAC Library) complete sequence.    38   6.0    1
AC003954|AC003954.1 Homo sapiens chromosome 5, Pac clone 162o17 (LBNL H147), complete sequence.    38   6.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q7YXD4) RecName: Full=Protein P80; AltName: Full=Endosomal memb...   336   3e-91
DQ646486_1(DQ646486|pid:none) Chlamydomonas reinhardtii transmem...    79   1e-13
Z68217_7(Z68217|pid:none) Caenorhabditis elegans Cosmid F58G6, c...    39   0.070
T32929(T32929)hypothetical protein K12C11.3 - Caenorhabditis ele...    36   0.59
DQ459355_1(DQ459355|pid:none) Drosophila melanogaster Ctr1B (Ctr...    34   2.2
Z79603_8(Z79603|pid:none) Caenorhabditis elegans Cosmid M163, co...    33   6.5

 psg: 0.83  gvh: 0.48  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHB478 is cyt

>VHB478Z.Seq
NNNNNNNNNNTGCGCATCAGGTTCAGTTGTTGANCAATGTTCATCAGTCGATTCAATTAG
TAATTTACCAACCNCAATGCAATTATTCGCTGGTATTAAAAGTATTTGCACAGAGATGGC
AATGGATGGTTGTGAGAAATGTTCAGGAAATTCACCAACTACCACCTGTGATGTTTTGCC
AGTTTACTCCTCCCTTTGTATGGCTATGCCTGATATGTCACAATGTGCCAATTGGACCAA
GATGTGTTCCTCCTCTGGTCAACTCTACAACTCTCAAATCACCACAGACTATTGTGTCGC
CTCTGTTGCTGACGCCGTTCCAATCATGAGAATGTACTTTCACACTGGTATCTTGGACTA
TATCCTCTTTAAATCTTGGGTACCAAGAACTGACCGTCAATTTGCTGGTTCATGGTNTGC
CATNNTNTTNTGTGCNATCTTTTTTGAACTCGAAAAAACCCTTAGAAGTATTTTAGAAAA
AAGATGGACTCCCAACAAAAAAGATTCANAAGATAATANTTTAATTAAGTTCATCTTTCC
TTAAGTGGTTTNTATC