>VHB460 (VHB460Q) /CSM/VH/VHB4-C/VHB460Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGXXXXXXXXXXTCATCCAAAGATGATCCAAA
AGCTCGTGCTAACTATCTCGCTGATAACCACGAATGGGATAAAAATGATGCCATGAACAT
TTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGT
TCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGA
AGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCT
CCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACT
CTATGCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAAT
TACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTAT
TGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACC
AGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTT
CCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGC
CACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCT
TGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTNAGTTTTCAAANATAGT
AATCAAATCTAAAAAAAA

LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVT---

---SSKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSF
VGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPT
LLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTAD
LRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti
*valxfsxivikskk

Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlkirnsvmsl---

---SSKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSF
VGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPT
LLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTAD
LRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti
*valxfsxivikskk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r*gir*ch---

---hpkmiqklvltislittngikmmp*tfglsvqkvtvpisssmspkvfni*mklkils
lvlsngppkkvlfvmkt*evsvsifmmspstlmpstevvvkssqllvvysmlln*lppql
s*nqst**kllpqktllvvftlysivvevlllvknvvsvphyslskliyqy*npsvspli
yvptlpvklshnvssiiglqlvlstkirkplksl*pperekv*pqksqiltnstknskqf
kshxsfqx**snlkk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVT---

---iqr*skssc*lsr**prmg*k*chehlvfrsrr*rcqsprqchqrcsife*n*rffr
wcfpmghqrrccl**kherypfqsl*chpph*chpqrwwsnhpncssctlcc*inclpns
lrtnllsrnycprkrywwyllctqss*rycyw*rtsyrfpiilcqssftsirilrfhr*f
tfphcrssfptmclrslgfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnl
srtxvfkxsnqi*kk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ491 (VHQ491Q) /CSM/VH/VHQ4-D/VHQ491Q.Seq.d/                       1475   0.0
VHQ434 (VHQ434Q) /CSM/VH/VHQ4-B/VHQ434Q.Seq.d/                       1475   0.0
VHQ410 (VHQ410Q) /CSM/VH/VHQ4-A/VHQ410Q.Seq.d/                       1475   0.0
VHQ212 (VHQ212Q) /CSM/VH/VHQ2-A/VHQ212Q.Seq.d/                       1475   0.0
VHQ208 (VHQ208Q) /CSM/VH/VHQ2-A/VHQ208Q.Seq.d/                       1475   0.0
VHQ186 (VHQ186Q) /CSM/VH/VHQ1-D/VHQ186Q.Seq.d/                       1475   0.0
VHP860 (VHP860Q) /CSM/VH/VHP8-C/VHP860Q.Seq.d/                       1475   0.0
VHP859 (VHP859Q) /CSM/VH/VHP8-C/VHP859Q.Seq.d/                       1475   0.0
VHP847 (VHP847Q) /CSM/VH/VHP8-B/VHP847Q.Seq.d/                       1475   0.0
VHP454 (VHP454Q) /CSM/VH/VHP4-C/VHP454Q.Seq.d/                       1475   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.  1326   0.0    3
AF107291|AF107291.1 Candida parapsilosis elongation factor 2 (EFT2) gene, partial cds.    72   1e-70  13
AF107286|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds.    78   2e-70  9
Y09664|Y09664.1 C.albicans EFT2 gene.                              78   6e-69  9
AF107289|AF107289.1 Candida tropicalis elongation factor 2 (EFT2) gene, partial cds.    70   9e-66  10
AF107290|AF107290.1 Candida lusitaniae elongation factor 2 (EFT2) gene, partial cds.    64   7e-49  8
AF534908|AF534908.2 Tetrahymena thermophila elongation factor 2 gene, complete cds.    84   5e-47  6
AY144999|AY144999.1 Saccharomyces kluyveri clone Contig1706 EFT2 gene, complete cds.    78   5e-45  7
AR550596|AR550596.1 Sequence 5727 from patent US 6747137.          78   3e-44  6
EH015431|EH015431.1 USDA-FP_188032 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt081_G08 5', mRNA sequence.    78   7e-43  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   303   1e-80
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   301   3e-80
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   298   3e-79
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   297   7e-79
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   296   9e-79
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   296   9e-79
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   296   9e-79
(P05197) RecName: Full=Elongation factor 2;          Short=EF-2;...   296   9e-79
AJ278147_1(AJ278147|pid:none) Rattus norvegicus partial Ef-2 gen...   296   9e-79
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   296   9e-79

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHB460 is cyt

>VHB460F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGNNNNNNNNNN

>VHB460Z.Seq
NNNNNNNNNNTCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGCTGATAACCA
CGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGC
CAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTT
CGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGG
TATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGG
TCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAAC
TCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTAT
TTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTC
CCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGA
TTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTC
AATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAG
AAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAAT
TTAAGTCGCACTTNAGTTTTCAAANATAGTAATCAAATCTAAAAAAAA

>VHB460P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTG----------TCATCCAAAGATGATCCAAA
AGCTCGTGCTAACTATCTCGCTGATAACCACGAATGGGATAAAAATGATGCCATGAACAT
TTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGT
TCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGA
AGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCT
CCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACT
CTATGCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAAT
TACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTAT
TGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACC
AGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTT
CCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGC
CACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCT
TGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTNAGTTTTCAAANATAGT
AATCAAATCTAAAAAAAA