>VHB458 (VHB458Q) /CSM/VH/VHB4-C/VHB458Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACX
XXXXXXXXXATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAA
CGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGA
TTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACAT
GAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGG
TGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTC
CCCAACTNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGG
TGGTATTTACTCTGTACTCAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTCGTAT
CGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCAC
CGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTG
GGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAG
AAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTA
AACAATTTAAGTCGCACTTTGAGTTTTCAAAAATAGGAATCAAATCTAAAAAAAATTTTA
AAAAAAAAA

LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGN---

---WDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENM
RGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTXLEPXYLVEITAPENAIG
GIYSVLNRRXGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHW
ASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*val*vfknrnqi*kkf*
kk

Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllv---

---mg*k*chehlvfrsrr*rcqsprqchqrcsife*n*rffrwcfpmghqrrccl**kh
erypfqsl*chpph*chpqrwwsnhpncssctlcc*inclpnxxrtnxlsrnycprkryw
wyllctqssxrycyw*rtsyrfpiilcqssftsirilrfhr*ftfphcrssfptmclrsl
gfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnlsrtlsfqk*esnlkkil
kkk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*---

---WDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENM
RGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTXLEPXYLVEITAPENAIG
GIYSVLNRRXGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHW
ASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*val*vfknrnqi*kkf*
kk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGN---

---gikmmp*tfglsvqkvtvpisssmspkvfni*mklkilslvlsngppkkvlfvmkt*
evsvsifmmspstlmpstevvvkssqllvvysmlln*lppqlx*nqxt**kllpqktllv
vftlysivvxvlllvknvvsvphyslskliyqy*npsvspliyvptlpvklshnvssiig
lqlvlstkirkplksl*pperekv*pqksqiltnstknskqfkshfefskigikskknfk
kk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB458 (VHB458Q) /CSM/VH/VHB4-C/VHB458Q.Seq.d/                       2468   0.0
VHP776 (VHP776Q) /CSM/VH/VHP7-D/VHP776Q.Seq.d/                       1354   0.0
VHO565 (VHO565Q) /CSM/VH/VHO5-C/VHO565Q.Seq.d/                       1354   0.0
VHI241 (VHI241Q) /CSM/VH/VHI2-B/VHI241Q.Seq.d/                       1354   0.0
VHA267 (VHA267Q) /CSM/VH/VHA2-C/VHA267Q.Seq.d/                       1354   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                       1352   0.0
VHP832 (VHP832Q) /CSM/VH/VHP8-B/VHP832Q.Seq.d/                       1352   0.0
VHP213 (VHP213Q) /CSM/VH/VHP2-A/VHP213Q.Seq.d/                       1352   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                       1352   0.0
VHH827 (VHH827Q) /CSM/VH/VHH8-B/VHH827Q.Seq.d/                       1352   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

EF059386|EF059386.1 Synthetic construct Saccharomyces cerevisiae clone FLH202901.01X EFT2, complete sequence.    68   6e-36  7
AR223730|AR223730.1 Sequence 3 from patent US 6440409.             68   3e-35  7
AR223729|AR223729.1 Sequence 1 from patent US 6440409.             68   5e-35  7
AB023579|AB023579.1 Plasmodium falciparum gene for elongation factor 2, partial cds.    76   2e-24  5
AC009894|AC009894.2 Arabidopsis thaliana chromosome I BAC T6H22 genomic sequence, complete sequence.    60   9e-18  5
AB026185|AB026185.1 Lithospermum erythrorhizon mRNA for elongation factor 2, partial cds.    42   0.001  2
AC009254|AC009254.8 Drosophila melanogaster, chromosome 2L, region 40A-40C, BAC clone BACR42E05, complete sequence.    46   0.003  2
AB026645|AB026645.1 Arabidopsis thaliana genomic DNA, chromosome 3, P1 clone: MGH6.    34   0.010  6
AB095183|AB095183.1 Cyanidioschyzon merolae gene for elongation factor-2, complete cds.    40   0.014  2
AR245261|AR245261.1 Sequence 620 from patent US 6476212.           40   0.092  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   280   1e-73
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   278   2e-73
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   277   5e-73
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   277   5e-73
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   276   9e-73
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   276   9e-73
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   276   1e-72
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   274   5e-72
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   274   5e-72
AK298498_1(AK298498|pid:none) Homo sapiens cDNA FLJ56548 complet...   274   5e-72

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHB458 is cyt

>VHB458F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACN
NNNNNNNNN

>VHB458Z.Seq
NNNNNNNNNNATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTA
ACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAG
ATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACA
TGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAG
GTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCT
CCCCAACTNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTG
GTGGTATTTACTCTGTACTCAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTCGTA
TCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCA
CCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATT
GGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCA
GAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCT
AAACAATTTAAGTCGCACTTTGAGTTTTCAAAAATAGGAATCAAATCTAAAAAAAATTTT
AAAAAAAAAA

>VHB458P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAAC-
---------ATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAA
CGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGA
TTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACAT
GAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGG
TGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTC
CCCAACTNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGG
TGGTATTTACTCTGTACTCAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTCGTAT
CGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCAC
CGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTG
GGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAG
AAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTA
AACAATTTAAGTCGCACTTTGAGTTTTCAAAAATAGGAATCAAATCTAAAAAAAATTTTA
AAAAAAAAA