>VHB412 (VHB412Q) /CSM/VH/VHB4-A/VHB412Q.Seq.d/
TTTATGATGGTTTTGGTGAATAAGCGGATCAACCACGGGTGAAATCTGTGCAAATTGGGC
AACTGATTTGTATAGCAAAGTAGTCCCTCTGGTCCCGTATTATGTCGACCAAGAACAGTT
TCAGGTGGGGAGTTTGGCTGGGGCGGCACATTTGTTAAAAGATAACGCAAGTGTCCAAAG
GCAGGCTCAGTGAGAACAGAAATCTCACGTAGAGTAAAAGGGCAAAAGCCTGCTTGATTC
TGATTTTCAGTACTAATCGGAACTGGGAAACCAGGGCCTATCGATCCTTTATGTGCTTAA
ATCTTAAGCCTXXXXXXXXXXGTAAATCAACCACCATTTGCATGTTTAGAAGATAACAAC
ACATGGGCATTAGTTGAAGATATTGAAAAGATTAGAGTTTTACTTGGAATTGATAATTGG
GTTGTGTTTGGAGGAAGTTGGGGTAGTACTCTCTCATTAGCATATGCTGAAACTCATCCA
TCAAGAGTTAAAGCATTGGTTTTAAGAGGTATTTTTACTTTAAGAAGAGAGGAATTAATT
TTCTTTTATCAAACTGGAGCAAGTTTCTTATTTGCAGATTATTTCGATGAATATTTAAAA
CCAATTCCACCAGCAGAAAGAGGTGATATCATTTCAGCCTACCATCGTAGATTAACAGGT
ACTGATGAAAAGATTAAACAAGAGGCAGCCAATGCATGGACAACATGGGAAATGGCAACA
TCAAGATTAATGGTTGATAAACATAAGATTGCACGTGGTGAAGATCCAATGTTTGCTTTA
GCATTTGCCAGAATTGAAAATCATTACTTTGTAAATGCCGGTTTCTTTAGAGAGGATGGT
CAATTGATTAACGATGCTCATATTCTTAAAAATATTCCAGGTGTTATCGTTCAAGGTAGA
TATGATGTAGTTTGCCCAATGAAATCCGCTTGGGATCTTAAAAAAGTTTGGGGTGAAAAT
GCCGATTTGGTTATCATTCCAGATTCCGGTCATTCTTGTTCCGAAAATGGTATCATTCAT
TCTTTAGTTGAAGCATGGATAAATTTAAACATCTTTAAAACATTTATTTTTTTTAATAAA
T

ydgfge*adqprvksvqigqliciak*slwsrimstknsfrwgvwlgrhic*kitqvskg
rlsenrnlt*SKRAKACLILIFSTNRNWETRAYRSFMCLNLKP---

---VNQPPFACLEDNNTWALVEDIEKIRVLLGIDNWVVFGGSWGSTLSLAYAETHPSRVK
ALVLRGIFTLRREELIFFYQTGASFLFADYFDEYLKPIPPAERGDIISAYHRRLTGTDEK
IKQEAANAWTTWEMATSRLMVDKHKIARGEDPMFALAFARIENHYFVNAGFFREDGQLIN
DAHILKNIPGVIVQGRYDVVCPMKSAWDLKKVWGENADLVIIPDSGHSCSENGIIHSLVE
AWINLNIFKTFIFFNK

Frame A:
fmmvlvnkrinhg*nlcklgn*fv*qsspsgpvlcrprtvsggefgwggtfvkr*rkcpk
agsvrteisrrvkgqkpa*f*fsvligtgkpgpidplca*ils---

---VNQPPFACLEDNNTWALVEDIEKIRVLLGIDNWVVFGGSWGSTLSLAYAETHPSRVK
ALVLRGIFTLRREELIFFYQTGASFLFADYFDEYLKPIPPAERGDIISAYHRRLTGTDEK
IKQEAANAWTTWEMATSRLMVDKHKIARGEDPMFALAFARIENHYFVNAGFFREDGQLIN
DAHILKNIPGVIVQGRYDVVCPMKSAWDLKKVWGENADLVIIPDSGHSCSENGIIHSLVE
AWINLNIFKTFIFFNK

Frame B:
l*wfw*isgsttgeicanwatdlyskvvplvpyyvdqeqfqvgslagaahllkdnasvqr
qaq*eqkshve*kgkslldsdfqy*selgnqglsilyvlks*a---

---*inhhlhv*kitthgh*lkilkrlefyleliiglcleevgvvlsh*hmlklihqelk
hwf*evfll*eern*fsfikleqvsylqiismni*nqfhqqkevisfqptivd*qvlmkr
lnkrqpmhgqhgkwqhqd*wlinirlhvvkiqcll*hlpelkiitl*mpvslermvn*lt
mliflkifqvlsfkvdmm*faq*nplgilkkfgvkmpiwlsfqipvilvpkmvsfil*lk
hg*i*tslkhlfflin

Frame C:
ydgfge*adqprvksvqigqliciak*slwsrimstknsfrwgvwlgrhic*kitqvskg
rlsenrnlt*SKRAKACLILIFSTNRNWETRAYRSFMCLNLKP---

---kstticmfrr*qhmgis*ry*kd*sftwn**lgcvwrklg*yslisic*nssiks*s
igfkryfyfkkrginfllsnwskflicrlfr*ifktnstsrkr*yhfslps*inry**kd
*trgsqcmdnmgngniking**t*dctw*rsnvcfsicqn*ksllckcrfl*rgwsid*r
csys*kysrcyrsr*i*cslpneirlgs*kslg*kcrfgyhsrfrsflfrkwyhsffs*s
mdkfkhl*niyff**

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB412 (VHB412Q) /CSM/VH/VHB4-A/VHB412Q.Seq.d/                       2054   0.0
VFH527 (VFH527Q) /CSM/VF/VFH5-B/VFH527Q.Seq.d/                       1445   0.0
VFH663 (VFH663Q) /CSM/VF/VFH6-C/VFH663Q.Seq.d/                       1439   0.0
VFK854 (VFK854Q) /CSM/VF/VFK8-C/VFK854Q.Seq.d/                       1429   0.0
VSH516 (VSH516Q) /CSM/VS/VSH5-A/VSH516Q.Seq.d/                       1427   0.0
VFN865 (VFN865Q) /CSM/VF/VFN8-C/VFN865Q.Seq.d/                       1423   0.0
VFA783 (VFA783Q) /CSM/VF/VFA7-D/VFA783Q.Seq.d/                       1362   0.0
VFJ844 (VFJ844Q) /CSM/VF/VFJ8-B/VFJ844Q.Seq.d/                       1283   0.0
VFE705 (VFE705Q) /CSM/VF/VFE7-A/VFE705Q.Seq.d/                       1027   0.0
VSC443 (VSC443Q) /CSM/VS/VSC4-B/VSC443Q.Seq.d/                        991   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC006837|AC006837.16 Arabidopsis thaliana chromosome 2 BAC F23H14 genomic sequence, complete sequence.    96   1e-32  2
AB021684|AB021684.1 Marchantia polymorpha genes for 26S rRNA, 5S rRNA, 18S rRNA, 5.8S rRNA and 26S rRNA.    96   4e-32  3
AC006763|AC006763.1 Caenorhabditis elegans clone Y42H9, *** SEQUENCING IN PROGRESS ***, 14 unordered pieces.   125   3e-30  2
AC004708|AC004708.1 CIC5B11.1  check: 4870  from: 1  to: 167234, complete sequence.    96   3e-30  2
DQ888227|DQ888227.1 Synthetic construct clone pNOY373 35S ribosomal RNA, 18S ribosomal RNA, 5.8S ribosomal RNA, 25S ribosomal RNA, and 5S ribosomal RNA, complete sequence.   125   3e-30  2
AB243296|AB243296.1 Schizosaccharomyces japonicus genes for 18S rRNA, ITS1, 5.8S rRNA, ITS2, 25S rRNA, complete sequence.   125   3e-30  2
AB103124|AB103124.1 Hormomya mutabilis gene for ITS2, 28S rRNA, partial sequence, country:Japan: Okinawa (Haneji inland sea).   125   1e-29  2
AB103121|AB103121.1 Urumella concava gene for ITS2, 28S rRNA, partial sequence, country:Japan: Okinawa (Shimoji island).   125   1e-29  2
AB103129|AB103129.1 Mytilus galloprovincialis gene for ITS2, 28S rRNA, partial sequence, country:Japan: central Honshu (Ooiso).   125   1e-29  2
AB103123|AB103123.1 Lithophaga curta gene for ITS2, 28S rRNA, partial sequence, country:Japan: central Honshu (Ooiso).   125   1e-29  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM743169_801(AM743169|pid:none) Stenotrophomonas maltophilia K27...   287   4e-76
CP001111_691(CP001111|pid:none) Stenotrophomonas maltophilia R55...   286   1e-75
CP000379_1256(CP000379|pid:none) Burkholderia cenocepacia AU 105...   285   1e-75
AP009386_839(AP009386|pid:none) Burkholderia multivorans ATCC 17...   284   4e-75
AP008229_3441(AP008229|pid:none) Xanthomonas oryzae pv. oryzae M...   283   6e-75
CP000525_864(CP000525|pid:none) Burkholderia mallei SAVP1 chromo...   283   1e-74
CP000011_1796(CP000011|pid:none) Burkholderia mallei ATCC 23344 ...   283   1e-74
AE008923_905(AE008923|pid:none) Xanthomonas axonopodis pv. citri...   282   1e-74
CP000085_2179(CP000085|pid:none) Burkholderia thailandensis E264...   281   3e-74
AM920689_3552(AM920689|pid:none) Xanthomonas campestris pv. camp...   281   4e-74

 psg: 0.96  gvh: 0.37  alm: 0.40  top: 0.40  tms: 0.00  mit: 0.38  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 28.0 %: mitochondrial
	 24.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VHB412 is cyt

>VHB412F.Seq
TTTATGATGGTTTTGGTGAATAAGCGGATCAACCACGGGTGAAATCTGTGCAAATTGGGC
AACTGATTTGTATAGCAAAGTAGTCCCTCTGGTCCCGTATTATGTCGACCAAGAACAGTT
TCAGGTGGGGAGTTTGGCTGGGGCGGCACATTTGTTAAAAGATAACGCAAGTGTCCAAAG
GCAGGCTCAGTGAGAACAGAAATCTCACGTAGAGTAAAAGGGCAAAAGCCTGCTTGATTC
TGATTTTCAGTACTAATCGGAACTGGGAAACCAGGGCCTATCGATCCTTTATGTGCTTAA
ATCTTAAGCCTNNNNNNNNNN

>VHB412Z.Seq
NNNNNNNNNNGTAAATCAACCACCATTTGCATGTTTAGAAGATAACAACACATGGGCATT
AGTTGAAGATATTGAAAAGATTAGAGTTTTACTTGGAATTGATAATTGGGTTGTGTTTGG
AGGAAGTTGGGGTAGTACTCTCTCATTAGCATATGCTGAAACTCATCCATCAAGAGTTAA
AGCATTGGTTTTAAGAGGTATTTTTACTTTAAGAAGAGAGGAATTAATTTTCTTTTATCA
AACTGGAGCAAGTTTCTTATTTGCAGATTATTTCGATGAATATTTAAAACCAATTCCACC
AGCAGAAAGAGGTGATATCATTTCAGCCTACCATCGTAGATTAACAGGTACTGATGAAAA
GATTAAACAAGAGGCAGCCAATGCATGGACAACATGGGAAATGGCAACATCAAGATTAAT
GGTTGATAAACATAAGATTGCACGTGGTGAAGATCCAATGTTTGCTTTAGCATTTGCCAG
AATTGAAAATCATTACTTTGTAAATGCCGGTTTCTTTAGAGAGGATGGTCAATTGATTAA
CGATGCTCATATTCTTAAAAATATTCCAGGTGTTATCGTTCAAGGTAGATATGATGTAGT
TTGCCCAATGAAATCCGCTTGGGATCTTAAAAAAGTTTGGGGTGAAAATGCCGATTTGGT
TATCATTCCAGATTCCGGTCATTCTTGTTCCGAAAATGGTATCATTCATTCTTTAGTTGA
AGCATGGATAAATTTAAACATCTTTAAAACATTTATTTTTTTTAATAAAT

>VHB412P.Seq
TTTATGATGGTTTTGGTGAATAAGCGGATCAACCACGGGTGAAATCTGTGCAAATTGGGC
AACTGATTTGTATAGCAAAGTAGTCCCTCTGGTCCCGTATTATGTCGACCAAGAACAGTT
TCAGGTGGGGAGTTTGGCTGGGGCGGCACATTTGTTAAAAGATAACGCAAGTGTCCAAAG
GCAGGCTCAGTGAGAACAGAAATCTCACGTAGAGTAAAAGGGCAAAAGCCTGCTTGATTC
TGATTTTCAGTACTAATCGGAACTGGGAAACCAGGGCCTATCGATCCTTTATGTGCTTAA
ATCTTAAGCCT----------GTAAATCAACCACCATTTGCATGTTTAGAAGATAACAAC
ACATGGGCATTAGTTGAAGATATTGAAAAGATTAGAGTTTTACTTGGAATTGATAATTGG
GTTGTGTTTGGAGGAAGTTGGGGTAGTACTCTCTCATTAGCATATGCTGAAACTCATCCA
TCAAGAGTTAAAGCATTGGTTTTAAGAGGTATTTTTACTTTAAGAAGAGAGGAATTAATT
TTCTTTTATCAAACTGGAGCAAGTTTCTTATTTGCAGATTATTTCGATGAATATTTAAAA
CCAATTCCACCAGCAGAAAGAGGTGATATCATTTCAGCCTACCATCGTAGATTAACAGGT
ACTGATGAAAAGATTAAACAAGAGGCAGCCAATGCATGGACAACATGGGAAATGGCAACA
TCAAGATTAATGGTTGATAAACATAAGATTGCACGTGGTGAAGATCCAATGTTTGCTTTA
GCATTTGCCAGAATTGAAAATCATTACTTTGTAAATGCCGGTTTCTTTAGAGAGGATGGT
CAATTGATTAACGATGCTCATATTCTTAAAAATATTCCAGGTGTTATCGTTCAAGGTAGA
TATGATGTAGTTTGCCCAATGAAATCCGCTTGGGATCTTAAAAAAGTTTGGGGTGAAAAT
GCCGATTTGGTTATCATTCCAGATTCCGGTCATTCTTGTTCCGAAAATGGTATCATTCAT
TCTTTAGTTGAAGCATGGATAAATTTAAACATCTTTAAAACATTTATTTTTTTTAATAAA
T