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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB385
Library VH
(Link to library)
Clone ID VHB385
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U14527-1
Original site URL
Representative seq. ID VHB385P
(Link to Original site)
Representative DNA sequence 
>VHB385 (VHB385Q) /CSM/VH/VHB3-D/VHB385Q.Seq.d/
AAAATTAAATAATTTTTTTTTATATATAATGGAACAAAAAGAATTTGATATTAAAAATAT
TTTATTGAAGATTGAAAAAGATAAAAATGAAACTTCAAAAAAAATACAAGAAAAATATAA
AGATTGTACAATTGAAGATTTGGAAAAATCTTTAAGAATCCAAAATAAAAAATCAGAAGA
AATTACTTTTATCTCAAAAAAGATTGAAGAATTAAATGAAAAATTAATAGTTGAAAAATA
TAAACCATTATTGTCATCGTCCAATAATTCAAATGAAATTGAAAATGAGATATCACCAAA
AATTAAAGAAATTTTAATTAAAAACTCTATATTAAAAAATGAAAATTCAGCAGTATCACC
ATCGATAACAACAACAGATGCATCAGCATCATTAAGTTTTGGGGGTAAAACAATTGTTTT
ACCATCAAATCAACCAACAACATTAAGAAAGAGTGATGCACCAGCTGATACAAAACATAT
TAAATTGGAAATTACCAATGATAATAAAATTAATATTATTTGGCAGGATGCAAAAAATTA
TCAATCAAAAGATTGGGTTGCACTTTATAACTATAAATATGCACCACCAGATGGTTATGT
AAATAATACATGGTATTGGGCTGGTAAXXXXXXXXXXGTCAAAAACTCAAACTTGCTCAG
TTTATAAACAATTATGTTTTGGTGTAAGATATTTTGATTTAAGAGTTGCAAGATTAAAAG
ATAAACTTTATATTATTCATAATTTTTACTCTGATTCAGTTAAACAAGTTTTAAAAGATA
TTCTTCAATACGTTTCAGAAAATGTAAATGAAGTTATCATTCTTCATTGGAGTCATCTTT
ATCTTGTCGATGAAGATAATAAATTATTAATGAAGATGATAATTGAAATATTAGGAAAAT
TCATGTCAAATAGTAATAAAGGACCAGATGTTAAAGTTGGAGATTTAGCTGGTACACCAA
TTATTTGTATATATGATGATTTAGTAAATCCATTAAGTAGTGGTGGTGCTGGTGGTAATG
GAAAAAGACCAGATATTCGTGATCCAAGACTTTGGGATCCTTCATGTATATCCTCACCTT
ATGAAACTTCAAGATATCATAGTTTCGAATCAATTTTAAAATTTTTAAAATCTAGAATTA
ATGTACCAAAAAGAAAAGTTCTCCATGTTTGTCAAGCAATTCTTACAATTGAATTTTCTT
ATGAATTCTTTGGACATGACTTAATAACTTGGACTGTAGAACATCGTAATAAATTCAATG
AATTCTTTAATGATCTTGAAACCTTTGCTTCACCAACAAATATTATAATGACTGATTTCG
TTACATTTTATCCATTATCTTCATATTGTATTCGTAGAAATACTTTAGAATTTAATAATA
GTAATAATTAGAATAA
sequence update 2002.10.25
Translated Amino Acid sequence 
KLNNFFLYIMEQKEFDIKNILLKIEKDKNETSKKIQEKYKDCTIEDLEKSLRIQNKKSEE
ITFISKKIEELNEKLIVEKYKPLLSSSNNSNEIENEISPKIKEILIKNSILKNENSAVSP
SITTTDASASLSFGGKTIVLPSNQPTTLRKSDAPADTKHIKLEITNDNKINIIWQDAKNY
QSKDWVALYNYKYAPPDGYVNNTWYWAG---

---SKTQTCSVYKQLCFGVRYFDLRVARLKDKLYIIHNFYSDSVKQVLKDILQYVSENVN
EVIILHWSHLYLVDEDNKLLMKMIIEILGKFMSNSNKGPDVKVGDLAGTPIICIYDDLVN
PLSSGGAGGNGKRPDIRDPRLWDPSCISSPYETSRYHSFESILKFLKSRINVPKRKVLHV
CQAILTIEFSYEFFGHDLITWTVEHRNKFNEFFNDLETFASPTNIIMTDFVTFYPLSSYC
IRRNTLEFNNSNN*n
Translated Amino Acid sequence (All Frames) 
Frame A:
kik*fffiyngtkri*y*kyfied*kr*k*nfkkntrki*rlyn*rfgkifknpk*kirr
nyfylkkd*rik*kins*ki*tiivivq*fk*n*k*ditkn*rnfn*klyikk*kfssit
idnnnrcisiikfwg*nncftikstnnikke*cts*ykty*ignyq***n*yylagckkl
sikrlgctl*l*icttrwlck*ymvlgw*---

---vknsnllsl*timfwckif*fksckikr*tlyys*fll*fs*tsfkryssirfrkck
*syhsslesslscr*r**iineddn*nirkihvk***rtrc*swrfswytnylyi**fsk
sik*wwcww*wkktrys*sktlgsfmyiltl*nfkis*frinfkifki*n*ctkkksspc
lssnsyn*ifl*ilwt*lnnldcrts**iq*il**s*nlcftnkyynd*fryilsiifil
ys*kyfri*****le*

Frame B:
KLNNFFLYIMEQKEFDIKNILLKIEKDKNETSKKIQEKYKDCTIEDLEKSLRIQNKKSEE
ITFISKKIEELNEKLIVEKYKPLLSSSNNSNEIENEISPKIKEILIKNSILKNENSAVSP
SITTTDASASLSFGGKTIVLPSNQPTTLRKSDAPADTKHIKLEITNDNKINIIWQDAKNY
QSKDWVALYNYKYAPPDGYVNNTWYWAG---

---SKTQTCSVYKQLCFGVRYFDLRVARLKDKLYIIHNFYSDSVKQVLKDILQYVSENVN
EVIILHWSHLYLVDEDNKLLMKMIIEILGKFMSNSNKGPDVKVGDLAGTPIICIYDDLVN
PLSSGGAGGNGKRPDIRDPRLWDPSCISSPYETSRYHSFESILKFLKSRINVPKRKVLHV
CQAILTIEFSYEFFGHDLITWTVEHRNKFNEFFNDLETFASPTNIIMTDFVTFYPLSSYC
IRRNTLEFNNSNN*n

Frame C:
n*iiffyi*wnkknlilkify*rlkkikmklqkkykknikivqlkiwknl*eskiknqkk
lllsqkrlkn*mkn**lkninhychrpiiqmklkmryhqklkkf*lktly*kmkiqqyhh
r*qqqmhqhh*vlgvkqlfyhqinqqh*ervmhqliqnilnwklpmiiklilfgrmqkii
nqkiglhfitinmhhqmvm*iihgiglv---

---qklklaqfinnyvlv*dili*elqd*kinfilfiiftliqlnkf*kiffntfqkm*m
klsffigvifilsmkiiny**r**lky*enscqivikdqmlklei*lvhqlfvymmi**i
h*vvvvlvvmekdqifviqdfgilhvyphlmklqdiivsnqf*nf*nlelmyqkekfsmf
vkqflqlnflmnsldmt**lgl*nivinsmnslmilkpllhqqil**lislhfihylhiv
fveil*nliiviiri
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB385 (VHB385Q) /CSM/VH/VHB3-D/VHB385Q.Seq.d/                       2020   0.0
VHA187 (VHA187Q) /CSM/VH/VHA1-D/VHA187Q.Seq.d/                       1443   0.0
VHJ542 (VHJ542Q) /CSM/VH/VHJ5-B/VHJ542Q.Seq.d/                       1292   0.0
SFL418 (SFL418Q) /CSM/SF/SFL4-A/SFL418Q.Seq.d/                       1285   0.0
VSK341 (VSK341Q) /CSM/VS/VSK3-B/VSK341Q.Seq.d/                       1281   0.0
VHI804 (VHI804Q) /CSM/VH/VHI8-A/VHI804Q.Seq.d/                       1267   0.0
VFC464 (VFC464Q) /CSM/VF/VFC4-C/VFC464Q.Seq.d/                        922   0.0
SSG273 (SSG273Q) /CSM/SS/SSG2-D/SSG273Q.Seq.d/                        795   0.0
VHE543 (VHE543Q) /CSM/VH/VHE5-B/VHE543Q.Seq.d/                        737   0.0
VHO505 (VHO505Q) /CSM/VH/VHO5-A/VHO505Q.Seq.d/                        555   e-157
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CU244100|CU244100.1 Equus caballus GSS, BAC clone CH241-387H24, T7 end sequence.    58   7e-04  1
AC117267|AC117267.2 Dictyostelium discoideum chromosome 2 map 5836255-5862024 strain AX4, complete sequence.    36   0.007  5
AX392737|AX392737.1 Sequence 27 from Patent WO0212526.             42   0.014  6
AR707084|AR707084.1 Sequence 27 from patent US 6933145.            42   0.014  6
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    38   0.032  11
DR702722|DR702722.1 Asn_02354 Aspergillus niger pBluescript (EcoRI-XhoI) Aspergillus niger cDNA clone Asn_02354, mRNA sequence.    52   0.043  1
EK385563|EK385563.1 1095469481565 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061005840167 3', genomic survey sequence.    52   0.043  1
U80927|U80927.1 Dictyostelium discoideum unknown protein gene, complete cds.    52   0.043  1
CF350581|CF350581.1 rl60f03.y1 Meloidogyne javanica J2 SMART pGEM Meloidogyne javanica cDNA 5' similar to TR:Q23555 Q23555 ZK637.8B PROTEIN. [1] ;, mRNA sequence.    46   0.050  2
AE014845|AE014845.1 Plasmodium falciparum 3D7 chromosome 12 section 2 of 9 of the complete sequence.    34   0.052  14
dna update 2007. 4. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54BH5) RecName: Full=PI-PLC X-box domain-containing protein DD...   504   e-141
(Q55DH0) RecName: Full=PI-PLC X domain-containing protein DDB_G0...   144   8e-33
U80927_1(U80927|pid:none) Dictyostelium discoideum unknown prote...   144   8e-33
BT081338_1(BT081338|pid:none) Caligus clemensi clone ccle-evs-51...    62   6e-08
BC042310_1(BC042310|pid:none) Xenopus laevis hypothetical protei...    60   2e-07
AY089577_1(AY089577|pid:none) Drosophila melanogaster RE10196 fu...    60   2e-07
AE014134_3303(AE014134|pid:none) Drosophila melanogaster chromos...    60   2e-07
CQ859810_1(CQ859810|pid:none) Sequence 101 from Patent WO2004072...    55   5e-06
AK038575_1(AK038575|pid:none) Mus musculus adult male hypothalam...    53   2e-05
BX648329_1(BX648329|pid:none) Homo sapiens mRNA; cDNA DKFZp686J1...    53   2e-05
protein update 2009. 6.27
PSORT 

 psg: 0.37  gvh: 0.02  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.09  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VHB385 is nuc
5' end seq. ID VHB385F
5' end seq. 
>VHB385F.Seq
AAAATTAAATAATTTTTTTTTATATATAATGGAACAAAAAGAATTTGATATTAAAAATAT
TTTATTGAAGATTGAAAAAGATAAAAATGAAACTTCAAAAAAAATACAAGAAAAATATAA
AGATTGTACAATTGAAGATTTGGAAAAATCTTTAAGAATCCAAAATAAAAAATCAGAAGA
AATTACTTTTATCTCAAAAAAGATTGAAGAATTAAATGAAAAATTAATAGTTGAAAAATA
TAAACCATTATTGTCATCGTCCAATAATTCAAATGAAATTGAAAATGAGATATCACCAAA
AATTAAAGAAATTTTAATTAAAAACTCTATATTAAAAAATGAAAATTCAGCAGTATCACC
ATCGATAACAACAACAGATGCATCAGCATCATTAAGTTTTGGGGGTAAAACAATTGTTTT
ACCATCAAATCAACCAACAACATTAAGAAAGAGTGATGCACCAGCTGATACAAAACATAT
TAAATTGGAAATTACCAATGATAATAAAATTAATATTATTTGGCAGGATGCAAAAAATTA
TCAATCAAAAGATTGGGTTGCACTTTATAACTATAAATATGCACCACCAGATGGTTATGT
AAATAATACATGGTATTGGGCTGGTAANNNNNNNNNN
Length of 5' end seq. 637
3' end seq. ID VHB385Z
3' end seq. 
>VHB385Z.Seq
NNNNNNNNNNGTCAAAAACTCAAACTTGCTCAGTTTATAAACAATTATGTTTTGGTGTAA
GATATTTTGATTTAAGAGTTGCAAGATTAAAAGATAAACTTTATATTATTCATAATTTTT
ACTCTGATTCAGTTAAACAAGTTTTAAAAGATATTCTTCAATACGTTTCAGAAAATGTAA
ATGAAGTTATCATTCTTCATTGGAGTCATCTTTATCTTGTCGATGAAGATAATAAATTAT
TAATGAAGATGATAATTGAAATATTAGGAAAATTCATGTCAAATAGTAATAAAGGACCAG
ATGTTAAAGTTGGAGATTTAGCTGGTACACCAATTATTTGTATATATGATGATTTAGTAA
ATCCATTAAGTAGTGGTGGTGCTGGTGGTAATGGAAAAAGACCAGATATTCGTGATCCAA
GACTTTGGGATCCTTCATGTATATCCTCACCTTATGAAACTTCAAGATATCATAGTTTCG
AATCAATTTTAAAATTTTTAAAATCTAGAATTAATGTACCAAAAAGAAAAGTTCTCCATG
TTTGTCAAGCAATTCTTACAATTGAATTTTCTTATGAATTCTTTGGACATGACTTAATAA
CTTGGACTGTAGAACATCGTAATAAATTCAATGAATTCTTTAATGATCTTGAAACCTTTG
CTTCACCAACAAATATTATAATGACTGATTTCGTTACATTTTATCCATTATCTTCATATT
GTATTCGTAGAAATACTTTAGAATTTAATAATAGTAATAATTAGAATAA
Length of 3' end seq. 769
Connected seq. ID VHB385P
Connected seq. 
>VHB385P.Seq
AAAATTAAATAATTTTTTTTTATATATAATGGAACAAAAAGAATTTGATATTAAAAATAT
TTTATTGAAGATTGAAAAAGATAAAAATGAAACTTCAAAAAAAATACAAGAAAAATATAA
AGATTGTACAATTGAAGATTTGGAAAAATCTTTAAGAATCCAAAATAAAAAATCAGAAGA
AATTACTTTTATCTCAAAAAAGATTGAAGAATTAAATGAAAAATTAATAGTTGAAAAATA
TAAACCATTATTGTCATCGTCCAATAATTCAAATGAAATTGAAAATGAGATATCACCAAA
AATTAAAGAAATTTTAATTAAAAACTCTATATTAAAAAATGAAAATTCAGCAGTATCACC
ATCGATAACAACAACAGATGCATCAGCATCATTAAGTTTTGGGGGTAAAACAATTGTTTT
ACCATCAAATCAACCAACAACATTAAGAAAGAGTGATGCACCAGCTGATACAAAACATAT
TAAATTGGAAATTACCAATGATAATAAAATTAATATTATTTGGCAGGATGCAAAAAATTA
TCAATCAAAAGATTGGGTTGCACTTTATAACTATAAATATGCACCACCAGATGGTTATGT
AAATAATACATGGTATTGGGCTGGTAA----------GTCAAAAACTCAAACTTGCTCAG
TTTATAAACAATTATGTTTTGGTGTAAGATATTTTGATTTAAGAGTTGCAAGATTAAAAG
ATAAACTTTATATTATTCATAATTTTTACTCTGATTCAGTTAAACAAGTTTTAAAAGATA
TTCTTCAATACGTTTCAGAAAATGTAAATGAAGTTATCATTCTTCATTGGAGTCATCTTT
ATCTTGTCGATGAAGATAATAAATTATTAATGAAGATGATAATTGAAATATTAGGAAAAT
TCATGTCAAATAGTAATAAAGGACCAGATGTTAAAGTTGGAGATTTAGCTGGTACACCAA
TTATTTGTATATATGATGATTTAGTAAATCCATTAAGTAGTGGTGGTGCTGGTGGTAATG
GAAAAAGACCAGATATTCGTGATCCAAGACTTTGGGATCCTTCATGTATATCCTCACCTT
ATGAAACTTCAAGATATCATAGTTTCGAATCAATTTTAAAATTTTTAAAATCTAGAATTA
ATGTACCAAAAAGAAAAGTTCTCCATGTTTGTCAAGCAATTCTTACAATTGAATTTTCTT
ATGAATTCTTTGGACATGACTTAATAACTTGGACTGTAGAACATCGTAATAAATTCAATG
AATTCTTTAATGATCTTGAAACCTTTGCTTCACCAACAAATATTATAATGACTGATTTCG
TTACATTTTATCCATTATCTTCATATTGTATTCGTAGAAATACTTTAGAATTTAATAATA
GTAATAATTAGAATAA
Length of connected seq. 1386
Full length Seq ID -
Full length Seq. -
Length of full length seq. -