>VHB376 (VHB376Q) /CSM/VH/VHB3-D/VHB376Q.Seq.d/
GAATTTAAGACTTGTAAAGATTCACACTTTTGTAAAAGAAATAGAGTTTCACATGAAGTT
GGAGTAATGAATGAAATGAAATCCAAACAAAATTTTAACATTGTAGAAGGTTCAATTAAA
TTGGTAAAGCAAGAGAATACCATTTACTTTGATTTACAAGAACAAAATCAAAAGAGTAAT
CTATTAACAATGAAACTTGAAATCTATGAAGGAGGTATCGTAAGAATGAGAGCTCAAGAA
AAAGAACCATTATTGAATAAGCAACGTTACCAAGTTCAAGATGTTTTATTGGATACAATT
AAAACCGTACCAATTCAATGGAAACAAGAACCTTCAAAACAATCAAACACTTTTTCATTT
AAACATGGTGAAAAGGAGTGTTGTTATGTATTAGTACAATTGGTACCATTTAAATTGGAT
GTTTACATTATGAATGAATTGGCAATTACAACCAATAGTGATAATCTATTCCATTTCGAA
CCAATCTCTGATAAACCACAACCATTACCACCAAAAGAAAAAAAATCTGAAGAAGAGAAT
AAAGAAGCTAATCAAGAAGAAGATAATAATAATAATAATAATGATAACAATGAAGAACAA
CAAGTTAGTAXXXXXXXXXXCCATACACTACCATCATTAGAGAGGCAATCGTCAAACGTT
ACTCTTACNTTCCATTATGGTATACCACTTTCTATCAAAATACACTCAATGGTGCTCCAG
TTATGAGACCACTTTGGGTTCAATATCCAAAAGAAGCCAATCTCTTTGATGTTGATGATC
ATTGTTTAATTGGTGATTCACTTTTAGTTAAACCAGTCACTCAACAATCTTGTAAAACTA
TGAAAGTCCTCTTACCAGGCCAAAGTGTCAATGAAATTTGGTATGATGTTAATACTGAGA
AACCAATCAATGCTGGTGTCATCGAAATTGATACTCCTTTAGAAAAGATTCCAGTTTATC
AACGTGGTGGTTCAATCATTTCAAAGAAAGAAAGAGTTCGTAGAAGTACCTATCAAATGA
GAGATGATCCATACACTATTCGTATCGCTTTGGACTCTTCAAAATCTGCTCAAGGTCAAT
TATACATTGATGATGAACACTCTTTTGATTATAAAAAAGGTAAATTCCTCTACAGACAAT
TCACTTTCAAAGATAATGTTCTCTCATTTTCTGATGCCTCAAATAAAAGCTCAACCTCTT
ACAAACCAAATGTTACCATTGAAAAAATCGTTATCCTTGGTGTTCAAAAACCTCACTCGA
TCACTTGTAACATTACTGGTAAAGAAAAATTATCATTTGAATATGATTCAACTCTTTCAA
AACTAACAATCAGAAAACCAGATTTATTAGTTGATACTGATTTTATTATAAAATTAAATT
AAATTAAAAAATAAATAAAAAGAAAACAAACAAATATAATAA

EFKTCKDSHFCKRNRVSHEVGVMNEMKSKQNFNIVEGSIKLVKQENTIYFDLQEQNQKSN
LLTMKLEIYEGGIVRMRAQEKEPLLNKQRYQVQDVLLDTIKTVPIQWKQEPSKQSNTFSF
KHGEKECCYVLVQLVPFKLDVYIMNELAITTNSDNLFHFEPISDKPQPLPPKEKKSEEEN
KEANQEEDNNNNNNDNNEEQQVS---

---PYTTIIREAIVKRYSYXPLWYTTFYQNTLNGAPVMRPLWVQYPKEANLFDVDDHCLI
GDSLLVKPVTQQSCKTMKVLLPGQSVNEIWYDVNTEKPINAGVIEIDTPLEKIPVYQRGG
SIISKKERVRRSTYQMRDDPYTIRIALDSSKSAQGQLYIDDEHSFDYKKGKFLYRQFTFK
DNVLSFSDASNKSSTSYKPNVTIEKIVILGVQKPHSITCNITGKEKLSFEYDSTLSKLTI
RKPDLLVDTDFIIKLN*ikk*ikrkqtnii

Frame A:
EFKTCKDSHFCKRNRVSHEVGVMNEMKSKQNFNIVEGSIKLVKQENTIYFDLQEQNQKSN
LLTMKLEIYEGGIVRMRAQEKEPLLNKQRYQVQDVLLDTIKTVPIQWKQEPSKQSNTFSF
KHGEKECCYVLVQLVPFKLDVYIMNELAITTNSDNLFHFEPISDKPQPLPPKEKKSEEEN
KEANQEEDNNNNNNDNNEEQQVS---

---PYTTIIREAIVKRYSYXPLWYTTFYQNTLNGAPVMRPLWVQYPKEANLFDVDDHCLI
GDSLLVKPVTQQSCKTMKVLLPGQSVNEIWYDVNTEKPINAGVIEIDTPLEKIPVYQRGG
SIISKKERVRRSTYQMRDDPYTIRIALDSSKSAQGQLYIDDEHSFDYKKGKFLYRQFTFK
DNVLSFSDASNKSSTSYKPNVTIEKIVILGVQKPHSITCNITGKEKLSFEYDSTLSKLTI
RKPDLLVDTDFIIKLN*ikk*ikrkqtnii

Frame B:
nlrlvkihtfvkeiefhmkle**mk*npnkiltl*kvqlnw*skripftliyknkikrvi
y*q*nlksmkevs*e*elkkknhy*isnvtkfkmfywiqlkpyqfngnknlqnnqtlfhl
nmvkrsvvmy*ynwyhlnwmftl*mnwqlqpiviiysisnqslinhnhyhqkkknlkkri
kklikkkiiiiiimitmknnklv---

---htlpslerqssnvtltfhygiplsikihsmvlql*dhfgfniqkkpislmlmiiv*l
vihf*lnqslnnlvkl*kssyqakvsmkfgmmlilrnqsmlvssklill*krfqfinvvv
qsfqrkkefvevpik*emihtlfvslwtlqnllkvnytlmmntlliikkvnsstdnslsk
imfshflmpqikaqpltnqmlplkkslslvfknltrslvtllvkknyhlnmiqlfqn*qs
enqiy*lilill*n*iklknk*kenkqi**

Frame C:
i*dl*rftll*kk*sft*swsne*neiqtkf*hcrrfn*igkareyhll*ftrtkske*s
innet*nl*rryrknessrkrtiie*atlpssrcfigyn*nrtnsmetrtfktikhffi*
tw*kgvllcistigti*igclhye*ignynq***sipfrtnl**tttittkrkki*rre*
rs*srrr********q*rtts*---

---ihyhh*rgnrqtlllxsimvyhflskytqwcssyettlgsiskrsqsl*c**slfnw
*ftfs*tshstil*nyespltrpkcq*nlv*c*y*etnqcwchrn*ysfrkdsslstwwf
nhfkerkss*kylsner*sihysyrfglfkicsrsiih***tlf*l*kr*iplqtihfqr
*cslif*clk*klnllqtkcyh*knrypwcsktsldhl*hyw*rkiii*i*fnsfktnnq
ktrfis*y*fyykikln*kinkkktnkyn

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB376 (VHB376Q) /CSM/VH/VHB3-D/VHB376Q.Seq.d/                       1493   0.0
AHM824 (AHM824Q) /CSM/AH/AHM8-A/AHM824Q.Seq.d/                       1370   0.0
VHK892 (VHK892Q) /CSM/VH/VHK8-D/VHK892Q.Seq.d/                       1366   0.0
VHA366 (VHA366Q) /CSM/VH/VHA3-C/VHA366Q.Seq.d/                       1362   0.0
AHF129 (AHF129Q) /CSM/AH/AHF1-B/AHF129Q.Seq.d/                       1273   0.0
AHN239 (AHN239Q) /CSM/AH/AHN2-B/AHN239Q.Seq.d/                       1261   0.0
VHF509 (VHF509Q) /CSM/VH/VHF5-A/VHF509Q.Seq.d/                       1221   0.0
VHC350 (VHC350Q) /CSM/VH/VHC3-C/VHC350Q.Seq.d/                       1209   0.0
VHF102 (VHF102Q) /CSM/VH/VHF1-A/VHF102Q.Seq.d/                       1203   0.0
AHL465 (AHL465Q) /CSM/AH/AHL4-C/AHL465Q.Seq.d/                       1203   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U72236|U72236.1 Dictyostelium discoideum ModA (modA) gene, complete cds.  1459   0.0    1
DV771667|DV771667.1 McClintock5_D06.ab1 Homarus EST library project Homarus americanus cDNA clone McClintock5_D06.ab1, mRNA sequence.    48   2e-04  3
CX994143| ENSANGP00000010269 - Anopheles gambiae. Score = 209 bits (532), Expect = 5e-53, mRNA sequence.    36   0.13   2
CX994233| ENSANGP00000010269 - Anopheles gambiae. Score = 199 bits (507), Expect = 4e-50, mRNA sequence.    36   0.13   2
CX994013| ModA - Dictyostelium discoideum. Score = 178 bits (451), Expect = 6e-44, mRNA sequence.    36   0.13   2
AC174093|AC174093.2 Strongylocentrotus purpuratus clone R3-43P24, WORKING DRAFT SEQUENCE, 11 unordered pieces.    44   0.89   4
DU945235|DU945235.1 376452 Tomato EcoRI BAC Library Solanum lycopersicum genomic clone SL_EcoRI0097I23 5, genomic survey sequence.    42   0.91   2
AC087174|AC087174.4 Homo sapiens chromosome 11, clone RP11-640J11, complete sequence.    36   2.2    8
AC184458|AC184458.1 Strongylocentrotus purpuratus clone R3-4003P19, WORKING DRAFT SEQUENCE, 23 unordered pieces.    44   2.4    5
CB077183|CB077183.1 hj50e05.g1 Hedyotis terminalis flower - Stage 2 (NYBG) Hedyotis terminalis cDNA clone hj50e05, mRNA sequence.    46   2.7    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q94502) RecName: Full=Neutral alpha-glucosidase AB;          EC...   496   e-139
BC130137_1(BC130137|pid:none) Xenopus laevis hypothetical protei...   207   1e-51
BC148865_1(BC148865|pid:none) Bos taurus glucosidase, alpha; neu...   205   3e-51
AK295899_1(AK295899|pid:none) Homo sapiens cDNA FLJ61290 complet...   200   9e-50
AK299819_1(AK299819|pid:none) Homo sapiens cDNA FLJ54057 complet...   200   9e-50
AF144074_1(AF144074|pid:none) Homo sapiens glucosidase II alpha ...   200   9e-50
BC017433_1(BC017433|pid:none) Homo sapiens glucosidase, alpha; n...   200   9e-50
AB173690_1(AB173690|pid:none) Macaca fascicularis brain cDNA clo...   198   5e-49
AB383744_1(AB383744|pid:none) Synthetic construct DNA, clone: pF...   198   5e-49
BC128069_1(BC128069|pid:none) Mus musculus alpha glucosidase 2 a...   196   1e-48

 psg: 0.39  gvh: 0.25  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.10  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial

>> prediction for VHB376 is cyt

>VHB376F.Seq
GAATTTAAGACTTGTAAAGATTCACACTTTTGTAAAAGAAATAGAGTTTCACATGAAGTT
GGAGTAATGAATGAAATGAAATCCAAACAAAATTTTAACATTGTAGAAGGTTCAATTAAA
TTGGTAAAGCAAGAGAATACCATTTACTTTGATTTACAAGAACAAAATCAAAAGAGTAAT
CTATTAACAATGAAACTTGAAATCTATGAAGGAGGTATCGTAAGAATGAGAGCTCAAGAA
AAAGAACCATTATTGAATAAGCAACGTTACCAAGTTCAAGATGTTTTATTGGATACAATT
AAAACCGTACCAATTCAATGGAAACAAGAACCTTCAAAACAATCAAACACTTTTTCATTT
AAACATGGTGAAAAGGAGTGTTGTTATGTATTAGTACAATTGGTACCATTTAAATTGGAT
GTTTACATTATGAATGAATTGGCAATTACAACCAATAGTGATAATCTATTCCATTTCGAA
CCAATCTCTGATAAACCACAACCATTACCACCAAAAGAAAAAAAATCTGAAGAAGAGAAT
AAAGAAGCTAATCAAGAAGAAGATAATAATAATAATAATAATGATAACAATGAAGAACAA
CAAGTTAGTANNNNNNNNNN

>VHB376Z.Seq
NNNNNNNNNNCCATACACTACCATCATTAGAGAGGCAATCGTCAAACGTTACTCTTACNT
TCCATTATGGTATACCACTTTCTATCAAAATACACTCAATGGTGCTCCAGTTATGAGACC
ACTTTGGGTTCAATATCCAAAAGAAGCCAATCTCTTTGATGTTGATGATCATTGTTTAAT
TGGTGATTCACTTTTAGTTAAACCAGTCACTCAACAATCTTGTAAAACTATGAAAGTCCT
CTTACCAGGCCAAAGTGTCAATGAAATTTGGTATGATGTTAATACTGAGAAACCAATCAA
TGCTGGTGTCATCGAAATTGATACTCCTTTAGAAAAGATTCCAGTTTATCAACGTGGTGG
TTCAATCATTTCAAAGAAAGAAAGAGTTCGTAGAAGTACCTATCAAATGAGAGATGATCC
ATACACTATTCGTATCGCTTTGGACTCTTCAAAATCTGCTCAAGGTCAATTATACATTGA
TGATGAACACTCTTTTGATTATAAAAAAGGTAAATTCCTCTACAGACAATTCACTTTCAA
AGATAATGTTCTCTCATTTTCTGATGCCTCAAATAAAAGCTCAACCTCTTACAAACCAAA
TGTTACCATTGAAAAAATCGTTATCCTTGGTGTTCAAAAACCTCACTCGATCACTTGTAA
CATTACTGGTAAAGAAAAATTATCATTTGAATATGATTCAACTCTTTCAAAACTAACAAT
CAGAAAACCAGATTTATTAGTTGATACTGATTTTATTATAAAATTAAATTAAATTAAAAA
ATAAATAAAAAGAAAACAAACAAATATAATAA

>VHB376P.Seq
GAATTTAAGACTTGTAAAGATTCACACTTTTGTAAAAGAAATAGAGTTTCACATGAAGTT
GGAGTAATGAATGAAATGAAATCCAAACAAAATTTTAACATTGTAGAAGGTTCAATTAAA
TTGGTAAAGCAAGAGAATACCATTTACTTTGATTTACAAGAACAAAATCAAAAGAGTAAT
CTATTAACAATGAAACTTGAAATCTATGAAGGAGGTATCGTAAGAATGAGAGCTCAAGAA
AAAGAACCATTATTGAATAAGCAACGTTACCAAGTTCAAGATGTTTTATTGGATACAATT
AAAACCGTACCAATTCAATGGAAACAAGAACCTTCAAAACAATCAAACACTTTTTCATTT
AAACATGGTGAAAAGGAGTGTTGTTATGTATTAGTACAATTGGTACCATTTAAATTGGAT
GTTTACATTATGAATGAATTGGCAATTACAACCAATAGTGATAATCTATTCCATTTCGAA
CCAATCTCTGATAAACCACAACCATTACCACCAAAAGAAAAAAAATCTGAAGAAGAGAAT
AAAGAAGCTAATCAAGAAGAAGATAATAATAATAATAATAATGATAACAATGAAGAACAA
CAAGTTAGTA----------CCATACACTACCATCATTAGAGAGGCAATCGTCAAACGTT
ACTCTTACNTTCCATTATGGTATACCACTTTCTATCAAAATACACTCAATGGTGCTCCAG
TTATGAGACCACTTTGGGTTCAATATCCAAAAGAAGCCAATCTCTTTGATGTTGATGATC
ATTGTTTAATTGGTGATTCACTTTTAGTTAAACCAGTCACTCAACAATCTTGTAAAACTA
TGAAAGTCCTCTTACCAGGCCAAAGTGTCAATGAAATTTGGTATGATGTTAATACTGAGA
AACCAATCAATGCTGGTGTCATCGAAATTGATACTCCTTTAGAAAAGATTCCAGTTTATC
AACGTGGTGGTTCAATCATTTCAAAGAAAGAAAGAGTTCGTAGAAGTACCTATCAAATGA
GAGATGATCCATACACTATTCGTATCGCTTTGGACTCTTCAAAATCTGCTCAAGGTCAAT
TATACATTGATGATGAACACTCTTTTGATTATAAAAAAGGTAAATTCCTCTACAGACAAT
TCACTTTCAAAGATAATGTTCTCTCATTTTCTGATGCCTCAAATAAAAGCTCAACCTCTT
ACAAACCAAATGTTACCATTGAAAAAATCGTTATCCTTGGTGTTCAAAAACCTCACTCGA
TCACTTGTAACATTACTGGTAAAGAAAAATTATCATTTGAATATGATTCAACTCTTTCAA
AACTAACAATCAGAAAACCAGATTTATTAGTTGATACTGATTTTATTATAAAATTAAATT
AAATTAAAAAATAAATAAAAAGAAAACAAACAAATATAATAA