>VHB365 (VHB365Q) /CSM/VH/VHB3-C/VHB365Q.Seq.d/
AAAAGTAATTGAAAATTGTATTTTATTTATTAAAGAATGAATAAAATTATTTATTTGTTT
GTTATTTTATTAATTTTTGGATATTTTAATGGTAAAATAATTTGCCAAAATGTTCCACCA
AAACAACATTCTGAAGCTATTCAATTACTAGCTCTTACAAAAGGCCCATCATATAATGCA
GTATGTACTTGGCTCACTTGCGCACCATTAAATGATGGTTCTGGTAATTATACAGTTACA
AAGATTAATTTATCACTTTTAAATGGTATCAAAAGTGCTTTTTTAGACATTGATTTCTCT
GTTTTTGAAAATGTTACAAGTATTACCCTTGGTCAAGGAATTTATCCATCTAAATTATTT
GACAGTTTAACAAAATTAACATCTTTAACAGTTTTAGATATTGATACATTTAATTCAACA
TATACAGATTCATTATTAGTTATGCCAAAAACAATGTATCTAATTAAAATTTTGAATATT
GGTACAAAATTAGGAGTTAATTTTTTTAAAAGTCCAGCCTATTATGTTTCGCTTTACAAT
GGTTTACCAGGTTATGAATTACCTTCTACTTTTCCAATTGATTTCCCAGTAAATGAAATT
AAATAATTTGGXXXXXXXXXXAATATACGGTACTGATTTTGGATGGTATATTAAAAATCC
ATTATATTTGAGAGTTGGTGTACCAAATAAATATCTTATTATTCCAGTTCCAATGGGTAT
TGGAAGAGGAGAGCAATCAACTGGATTTACGAATGATAAATTTCTCTCATTTAACTATAC
ATATGAAGAACCAATAATTACGTCATTCTCTTACAATAGTACCGGTACTCCAAAACTTTT
AATTACTGGTTCTGGATTTAATTTTATTGGAAAAAATAATGTTACCATAGATGGTGTAGT
TTATACTTTTTCACAAGCATATACTTATCAATACAATGGTTCAATTTATCCTACCGTTTT
AATGAATAATTTTCCAAATGGTAACAAATTTACAGTATCAATTAATGTTGGTGGTGTTCA
ATCAAAAAATGTTACATTTGATTTAGATTCTTCTTCAGGAGGTAGTTCAACTACAGCTGG
TCCAACTACAGCTGGTTCAACTACAGCTGGTTCAACAACATCAGTAACTTCAGGAAGTGG
TTCAACAATAGGTGGTTCAACAACATCAAGTTCTCAAACAACTTCATCTAAATCCTCCTC
AACTGATGACTCATCAAATCATCCTTCCTCATCTTCTATACTTAATGTATCACTCATTTC
ATTTATATTTTTAATTATTGCTTCAATTATTATATAATTAAATAATAATAATAATAAGGA
ATATTTAAATTGTAATAAAATTATATNATTTAACAATTACCAAA

kviencilfike*iklficllfy*fldilmvk*fakmfhqnnilklfny*llqkahhimq
yvlgslahh*mmvlviiqlqrliyhf*mvskvlf*tlislflkmlqvlplvkefihlnyl
tv*qn*hl*qf*ilihliqhiqihy*lcqkqci*lkf*ilvqn*eliflkvqpimfrftm
vyqvmnylllfqlisq*MKLNNL---

---IYGTDFGWYIKNPLYLRVGVPNKYLIIPVPMGIGRGEQSTGFTNDKFLSFNYTYEEP
IITSFSYNSTGTPKLLITGSGFNFIGKNNVTIDGVVYTFSQAYTYQYNGSIYPTVLMNNF
PNGNKFTVSINVGGVQSKNVTFDLDSSSGGSSTTAGPTTAGSTTAGSTTSVTSGSGSTIG
GSTTSSSQTTSSKSSSTDDSSNHPSSSSILNVSLISFIFLIIASIII*lnnnnnkeylnc
nkiixfnnyq

Frame A:
ksn*klyfiy*rmnkiiylfvillifgyfngkiicqnvppkqhseaiqllaltkgpsyna
vctwltcaplndgsgnytvtkinlsllngiksafldidfsvfenvtsitlgqgiypsklf
dsltkltsltvldidtfnstytdsllvmpktmylikilnigtklgvnffkspayyvslyn
glpgyelpstfpidfpvneik*f---

---niry*fwmvy*ksiifeswctk*isyysssngywkrrainwiye**isli*lyi*rt
nnyvillq*yrysktfnywfwi*fywkk*cyhrwcslyfftsiylsiqwfnlsyrfne*f
skw*qiysin*cwwcsikkcyi*frfffrr*fnyswsnyswfnyswfnnisnfrkwfnnr
wfnnikfsnnfi*illn**likssflifyt*cithfiyifnycfnyyiik*****gifkl
**nyxi*qlp

Frame B:
kviencilfike*iklficllfy*fldilmvk*fakmfhqnnilklfny*llqkahhimq
yvlgslahh*mmvlviiqlqrliyhf*mvskvlf*tlislflkmlqvlplvkefihlnyl
tv*qn*hl*qf*ilihliqhiqihy*lcqkqci*lkf*ilvqn*eliflkvqpimfrftm
vyqvmnylllfqlisq*MKLNNL---

---IYGTDFGWYIKNPLYLRVGVPNKYLIIPVPMGIGRGEQSTGFTNDKFLSFNYTYEEP
IITSFSYNSTGTPKLLITGSGFNFIGKNNVTIDGVVYTFSQAYTYQYNGSIYPTVLMNNF
PNGNKFTVSINVGGVQSKNVTFDLDSSSGGSSTTAGPTTAGSTTAGSTTSVTSGSGSTIG
GSTTSSSQTTSSKSSSTDDSSNHPSSSSILNVSLISFIFLIIASIII*lnnnnnkeylnc
nkiixfnnyq

Frame C:
k*lkivfyllkne*nylfvcyfinfwif*w*nnlpkcstkttf*sysitssykrpii*cs
mylahlrtik*wfw*lysykd*fitfkwyqkcffrh*flcf*kcykyypwsrnlsi*ii*
qfnkinifnsfry*yi*fniyrfiisyaknnvsn*nfeywykirs*ff*kssllcfalqw
ftrl*itfyfsn*fpsk*n*iiw---

---ytvlildgilkihyi*elvyqinillfqfqwvleeesnqldlrminfshltihmknq
*lrhsltivpvlqnf*llvldlillekimlp*mv*filfhkhilintmvqfilpf**iif
qmvtnlqyqlmlvvfnqkmlhli*illqevvqlqlvqlqlvqlqlvqqhq*lqevvqq*v
vqqhqvlkqlhlnppqlmthqiilphllylmyhsfhlyf*lllqllyn*iiiiirni*iv
iklyxltitk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB365 (VHB365Q) /CSM/VH/VHB3-C/VHB365Q.Seq.d/                       2270   0.0
VHQ460 (VHQ460Q) /CSM/VH/VHQ4-C/VHQ460Q.Seq.d/                       1306   0.0
VHP317 (VHP317Q) /CSM/VH/VHP3-A/VHP317Q.Seq.d/                       1306   0.0
VHJ452 (VHJ452Q) /CSM/VH/VHJ4-C/VHJ452Q.Seq.d/                       1306   0.0
VHB781 (VHB781Q) /CSM/VH/VHB7-D/VHB781Q.Seq.d/                       1306   0.0
VHL849 (VHL849Q) /CSM/VH/VHL8-C/VHL849Q.Seq.d/                       1257   0.0
VHJ263 (VHJ263Q) /CSM/VH/VHJ2-C/VHJ263Q.Seq.d/                       1251   0.0
VHE537 (VHE537Q) /CSM/VH/VHE5-B/VHE537Q.Seq.d/                       1241   0.0
SSE735 (SSE735Q) /CSM/SS/SSE7-B/SSE735Q.Seq.d/                       1229   0.0
VHE612 (VHE612Q) /CSM/VH/VHE6-A/VHE612Q.Seq.d/                       1187   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CD305901|CD305901.1 StrPu691.002266 Sea urchin larva cDNA library MPMGp691MPMGp691L1533;MPI_SURUDI_33L15 5', mRNA sequence.    54   0.003  2
CD307097|CD307097.1 StrPu691.009464 Sea urchin larva cDNA library MPMGp691 Strongylocentrotus purpuratus cDNA clone MPMGp691E0444;MPI_SURUDI_44E4 5', mRNA sequence.    54   0.003  2
CD305891|CD305891.1 StrPu691.002256 Sea urchin larva cDNA library MPMGp691 Strongylocentrotus purpuratus cDNA clone MPMGp691M2232;MPI_SURUDI_32M22 5', mRNA sequence.    54   0.003  2
AZ530575|AZ530575.1 ENTCR83TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    36   0.12   2
AC178991|AC178991.1 Strongylocentrotus purpuratus clone R3-3122K12, WORKING DRAFT SEQUENCE, 21 unordered pieces.    50   0.17   1
AC174877|AC174877.2 Strongylocentrotus purpuratus clone R3-1107I22, WORKING DRAFT SEQUENCE, 10 unordered pieces.    50   0.17   1
AC168831|AC168831.2 Strongylocentrotus purpuratus clone R3-13D18, WORKING DRAFT SEQUENCE, 30 unordered pieces.    50   0.17   1
CZ998807|CZ998807.1 188282 Tomato MboI BAC Library Solanum lycopersicum genomic clone SL_MboI0079M06 5, genomic survey sequence.    50   0.17   1
EK125616|EK125616.1 1092978801086 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061002085337 3', genomic survey sequence.    32   0.19   3
ER516504|ER516504.1 1093015518982 Global-Ocean-Sampling_GS-35-01-01-1P5KB marine metagenome genomic clone 1061002440372 3', genomic survey sequence.    44   0.24   2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB016609_1(AB016609|pid:none) Dictyostelium discoideum gene for ...    65   6e-09
AC115599_1(AC115599|pid:none) Dictyostelium discoideum chromosom...    48   0.001
AP004126_12(AP004126|pid:none) Oryza sativa Japonica Group genom...    40   0.19
AC158497_15(AC158497|pid:none) Medicago truncatula chromosome 2 ...    38   0.74
CP001022_2156(CP001022|pid:none) Exiguobacterium sibiricum 255-1...    38   0.97
AC116984_31(AC116984|pid:none) Dictyostelium discoideum chromoso...    37   1.3
AL646053_351(AL646053|pid:none) Ralstonia solanacearum GMI1000 m...    35   6.3
AB232984_1(AB232984|pid:none) Physarum polycephalum BglM3 mRNA f...    35   8.2

 psg: 0.70  gvh: 0.31  alm: 0.19  top: 0.57  tms: 0.07  mit: 0.14  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.02  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 16.0 %: nuclear
	 16.0 %: vesicles of secretory system
	 12.0 %: plasma membrane
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  8.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHB365 is cyt

>VHB365F.Seq
AAAAGTAATTGAAAATTGTATTTTATTTATTAAAGAATGAATAAAATTATTTATTTGTTT
GTTATTTTATTAATTTTTGGATATTTTAATGGTAAAATAATTTGCCAAAATGTTCCACCA
AAACAACATTCTGAAGCTATTCAATTACTAGCTCTTACAAAAGGCCCATCATATAATGCA
GTATGTACTTGGCTCACTTGCGCACCATTAAATGATGGTTCTGGTAATTATACAGTTACA
AAGATTAATTTATCACTTTTAAATGGTATCAAAAGTGCTTTTTTAGACATTGATTTCTCT
GTTTTTGAAAATGTTACAAGTATTACCCTTGGTCAAGGAATTTATCCATCTAAATTATTT
GACAGTTTAACAAAATTAACATCTTTAACAGTTTTAGATATTGATACATTTAATTCAACA
TATACAGATTCATTATTAGTTATGCCAAAAACAATGTATCTAATTAAAATTTTGAATATT
GGTACAAAATTAGGAGTTAATTTTTTTAAAAGTCCAGCCTATTATGTTTCGCTTTACAAT
GGTTTACCAGGTTATGAATTACCTTCTACTTTTCCAATTGATTTCCCAGTAAATGAAATT
AAATAATTTGGNNNNNNNNNN

>VHB365Z.Seq
NNNNNNNNNNAATATACGGTACTGATTTTGGATGGTATATTAAAAATCCATTATATTTGA
GAGTTGGTGTACCAAATAAATATCTTATTATTCCAGTTCCAATGGGTATTGGAAGAGGAG
AGCAATCAACTGGATTTACGAATGATAAATTTCTCTCATTTAACTATACATATGAAGAAC
CAATAATTACGTCATTCTCTTACAATAGTACCGGTACTCCAAAACTTTTAATTACTGGTT
CTGGATTTAATTTTATTGGAAAAAATAATGTTACCATAGATGGTGTAGTTTATACTTTTT
CACAAGCATATACTTATCAATACAATGGTTCAATTTATCCTACCGTTTTAATGAATAATT
TTCCAAATGGTAACAAATTTACAGTATCAATTAATGTTGGTGGTGTTCAATCAAAAAATG
TTACATTTGATTTAGATTCTTCTTCAGGAGGTAGTTCAACTACAGCTGGTCCAACTACAG
CTGGTTCAACTACAGCTGGTTCAACAACATCAGTAACTTCAGGAAGTGGTTCAACAATAG
GTGGTTCAACAACATCAAGTTCTCAAACAACTTCATCTAAATCCTCCTCAACTGATGACT
CATCAAATCATCCTTCCTCATCTTCTATACTTAATGTATCACTCATTTCATTTATATTTT
TAATTATTGCTTCAATTATTATATAATTAAATAATAATAATAATAAGGAATATTTAAATT
GTAATAAAATTATATNATTTAACAATTACCAAA

>VHB365P.Seq
AAAAGTAATTGAAAATTGTATTTTATTTATTAAAGAATGAATAAAATTATTTATTTGTTT
GTTATTTTATTAATTTTTGGATATTTTAATGGTAAAATAATTTGCCAAAATGTTCCACCA
AAACAACATTCTGAAGCTATTCAATTACTAGCTCTTACAAAAGGCCCATCATATAATGCA
GTATGTACTTGGCTCACTTGCGCACCATTAAATGATGGTTCTGGTAATTATACAGTTACA
AAGATTAATTTATCACTTTTAAATGGTATCAAAAGTGCTTTTTTAGACATTGATTTCTCT
GTTTTTGAAAATGTTACAAGTATTACCCTTGGTCAAGGAATTTATCCATCTAAATTATTT
GACAGTTTAACAAAATTAACATCTTTAACAGTTTTAGATATTGATACATTTAATTCAACA
TATACAGATTCATTATTAGTTATGCCAAAAACAATGTATCTAATTAAAATTTTGAATATT
GGTACAAAATTAGGAGTTAATTTTTTTAAAAGTCCAGCCTATTATGTTTCGCTTTACAAT
GGTTTACCAGGTTATGAATTACCTTCTACTTTTCCAATTGATTTCCCAGTAAATGAAATT
AAATAATTTGG----------AATATACGGTACTGATTTTGGATGGTATATTAAAAATCC
ATTATATTTGAGAGTTGGTGTACCAAATAAATATCTTATTATTCCAGTTCCAATGGGTAT
TGGAAGAGGAGAGCAATCAACTGGATTTACGAATGATAAATTTCTCTCATTTAACTATAC
ATATGAAGAACCAATAATTACGTCATTCTCTTACAATAGTACCGGTACTCCAAAACTTTT
AATTACTGGTTCTGGATTTAATTTTATTGGAAAAAATAATGTTACCATAGATGGTGTAGT
TTATACTTTTTCACAAGCATATACTTATCAATACAATGGTTCAATTTATCCTACCGTTTT
AATGAATAATTTTCCAAATGGTAACAAATTTACAGTATCAATTAATGTTGGTGGTGTTCA
ATCAAAAAATGTTACATTTGATTTAGATTCTTCTTCAGGAGGTAGTTCAACTACAGCTGG
TCCAACTACAGCTGGTTCAACTACAGCTGGTTCAACAACATCAGTAACTTCAGGAAGTGG
TTCAACAATAGGTGGTTCAACAACATCAAGTTCTCAAACAACTTCATCTAAATCCTCCTC
AACTGATGACTCATCAAATCATCCTTCCTCATCTTCTATACTTAATGTATCACTCATTTC
ATTTATATTTTTAATTATTGCTTCAATTATTATATAATTAAATAATAATAATAATAAGGA
ATATTTAAATTGTAATAAAATTATATNATTTAACAATTACCAAA