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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB353
Library VH
(Link to library)
Clone ID VHB353
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12097-1
Original site URL
Representative seq. ID VHB353P
(Link to Original site)
Representative DNA sequence 
>VHB353 (VHB353Q) /CSM/VH/VHB3-C/VHB353Q.Seq.d/
ACTGTTGGCCTACTGGNAATAATAATAGCTGAAGCAATAGTATCTCATTTAAGTAATAAA
AAAAAATGGATCCAATTATTCAAACCATTCAATTATTCCAACATACTCTTCATCATGACG
CTAATGTCATTAAAGCTGCCGAAGCTCAACTTCAACAAATTAAAGTAACTGATGGATATT
CAAGAATTTTATTAAAAATTTTAGCATCAAATGAAGTTGATATTTCAATTAGACAAGGTG
TTTCAATTTTTTTAAAGAATATGATCATTACAAAATGGAGAGGTGCTGAAGATGAATCAC
CAATCACTCAAGAGGATGCAGAATTCATTAAAGAGAATCTCATTGATCTTTTAGTTCATT
CACATCATTTAGTTCAAAATCAAATTGAAGCAATGATTGAAATTATTGCAAATCGTGATT
TCCCTGAAAAATGGACTTCATTATTACCAAAATCAATTCAATATATTAATACTCAAGATG
TTAAATTAATTTTAGCTGGTTTAACTTCAATTCAATTAGGTATTAAAAGATTTAGATATG
TAACAATGGGTGATAAAAAAAAAXXXXXXXXXXATGGTTTAATAGAATTAAACATTTCCA
AAGATTCTATGATAAAAAGATCTCAGTTTTAGCATTCTCATCATTACTTACCCTTAATCC
AAGTCCAAATTTCGTTAAATTTGGTACATCTTTAATCTTGGAGAAAATGTTACAATTCAC
CAAAGATATGTTATCCATTGAAAAGGAATTAGATAAACAAGAGGCTGAACGTGAACAAAA
AATTAAAGATGGTACACTTAAACCAGAGGAAGAAGAATTCATTGATGAAAATGATGAAGA
AGATTATTTTGATAATCATAAATTTGAATTTGAATTCACTGAAATTCCAGATAATCAAGA
TTGTCAACATGATGATGAAGGTGAAGTATTCTTGGATGATATTGAAAAAGCAACTGAATA
CTTTGAAAATGGTGGTGATTTAGGTGAAGATGAAGGTGATAACTTTGATGATCAAAACGA
CGATGATGATCAAGATTCTGAAGAAGATCTCTTTGAAGATGAAGATACTCCAGACTTTGA
AACCCCAATTGATGAAGTTGATGGTTTCGAATTTATGATTAATTCAATTCAAAATTTCTT
CCAAATTAATCCAACTTGTATTCAACAAATCTCGAAAAACAACAAGAAAAAATTAAAAAA
TAT
sequence update 2002.10.25
Translated Amino Acid sequence 
cwptgnnns*snsisfk**KKMDPIIQTIQLFQHTLHHDANVIKAAEAQLQQIKVTDGYS
RILLKILASNEVDISIRQGVSIFLKNMIITKWRGAEDESPITQEDAEFIKENLIDLLVHS
HHLVQNQIEAMIEIIANRDFPEKWTSLLPKSIQYINTQDVKLILAGLTSIQLGIKRFRYV
TMGDKKK---

---WFNRIKHFQRFYDKKISVLAFSSLLTLNPSPNFVKFGTSLILEKMLQFTKDMLSIEK
ELDKQEAEREQKIKDGTLKPEEEEFIDENDEEDYFDNHKFEFEFTEIPDNQDCQHDDEGE
VFLDDIEKATEYFENGGDLGEDEGDNFDDQNDDDDQDSEEDLFEDEDTPDFETPIDEVDG
FEFMINSIQNFFQINPTCIQQISKNNKKKLKN
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxiiiaeaivshlsnkkkwiqlfkpfnysnilfimtlmslklpklnfnklk*lmdi
qefy*kf*hqmklifqldkvfqff*ri*slqngevlkmnhqslkrmqnslkrislif*fi
hii*fkiklkq*lkllqivislknglhyyqnqfnililkmln*f*lv*lqfn*vlkdldm
*qwvikk---

---mv**n*tfpkil**kdlsfsiliityp*skskfr*iwyifnlgenvtihqryvih*k
gir*trg*t*tkn*rwyt*trgrrih**k**rrlf**s*i*i*ih*nsr*srlst***r*
silg*y*ksn*il*kww*fr*r*r**l**skrr**srf*rrsl*r*rysrl*npn**s*w
friyd*fnskflpn*snlystnlekqqekikky

Frame B:
llaywx***lkq*yli*vikkngsnysnhsiiptysss*r*ch*scrsststn*sn*wif
knfiknfsik*s*yfn*trcfnffkeydhykmerc*r*itnhsrgcrih*resh*sfssf
tsfssksn*snd*nycks*fp*kmdfiitkinsiy*ysrc*infswfnfnsiry*ki*ic
nng**kk---

---WFNRIKHFQRFYDKKISVLAFSSLLTLNPSPNFVKFGTSLILEKMLQFTKDMLSIEK
ELDKQEAEREQKIKDGTLKPEEEEFIDENDEEDYFDNHKFEFEFTEIPDNQDCQHDDEGE
VFLDDIEKATEYFENGGDLGEDEGDNFDDQNDDDDQDSEEDLFEDEDTPDFETPIDEVDG
FEFMINSIQNFFQINPTCIQQISKNNKKKLKN

Frame C:
cwptgnnns*snsisfk**KKMDPIIQTIQLFQHTLHHDANVIKAAEAQLQQIKVTDGYS
RILLKILASNEVDISIRQGVSIFLKNMIITKWRGAEDESPITQEDAEFIKENLIDLLVHS
HHLVQNQIEAMIEIIANRDFPEKWTSLLPKSIQYINTQDVKLILAGLTSIQLGIKRFRYV
TMGDKKK---

---glielniskdsmikrsqf*hshhylpliqvqislnlvhl*swrkcynspkicyplkr
n*inkrlnvnkklkmvhlnqrkknslmkmmkkiiliiinlnlnslkfqiikivnmmmkvk
yswmilkkqlntlkmvvi*vkmkvitlmikttmmikilkkislkmkilqtlkpqlmklmv
snl*liqfkisskliqlvfnksrkttrkn*ki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB353 (VHB353Q) /CSM/VH/VHB3-C/VHB353Q.Seq.d/                       1191   0.0
VHQ118 (VHQ118Q) /CSM/VH/VHQ1-A/VHQ118Q.Seq.d/                       1189   0.0
VHP869 (VHP869Q) /CSM/VH/VHP8-C/VHP869Q.Seq.d/                       1189   0.0
VHP585 (VHP585Q) /CSM/VH/VHP5-D/VHP585Q.Seq.d/                       1189   0.0
VHP171 (VHP171Q) /CSM/VH/VHP1-C/VHP171Q.Seq.d/                       1189   0.0
VHO487 (VHO487Q) /CSM/VH/VHO4-D/VHO487Q.Seq.d/                       1189   0.0
VHO210 (VHO210Q) /CSM/VH/VHO2-A/VHO210Q.Seq.d/                       1189   0.0
VHM366 (VHM366Q) /CSM/VH/VHM3-C/VHM366Q.Seq.d/                       1189   0.0
VHI645 (VHI645Q) /CSM/VH/VHI6-B/VHI645Q.Seq.d/                       1189   0.0
VHH875 (VHH875Q) /CSM/VH/VHH8-D/VHH875Q.Seq.d/                       1189   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC006746|AC006746.1 Caenorhabditis elegans clone Y39A3, *** SEQUENCING IN PROGRESS ***,2 unordered pieces.    34   0.037  6
AC010025|AC010025.6 Drosophila melanogaster 3L BAC RPCI98-20B1 (Roswell Park Cancer Institute Drosophila BAC Library) complete sequence.    44   0.21   1
AE005672|AE005672.2 Streptococcus pneumoniae TIGR4, complete genome.    34   0.44   11
AC005882|AC005882.1 Arabidopsis thaliana chromosome I BAC T13M11 genomic sequence, complete sequence.    36   0.65   3
AC084582|AC084582.1 Caenorhabditis briggsae cosmid G40M04, complete sequence.    38   0.67   3
AC006771|AC006771.1 Caenorhabditis elegans clone Y46C8, *** SEQUENCING IN PROGRESS ***, 8 unordered pieces.    38   0.84   4
AC009801|AC009801.4 Homo sapiens chromosome 8 clone RP11-399D24 map 8, *** SEQUENCING IN PROGRESS ***, 123 unordered pieces.    42   0.85   1
AC068609|AC068609.3 Mus musculus strain C57BL6/J chromosome 5 clone RP23-98L5, complete sequence.    42   0.85   1
AC004756|AC004756.1 Homo sapiens chromosome 17, clone hCIT.508_D_18, complete sequence.    42   0.85   1
AC005139|AC005139.3 Plasmodium falciparum chromosome 12, WORKING DRAFT SEQUENCE, 5 unordered pieces.    40   0.89   4
dna update 2007. 4. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55CX9) RecName: Full=Probable importin-7 homolog;                   325   1e-87
CP001333_13(CP001333|pid:none) Micromonas sp. RCC299 chromosome ...    88   7e-16
CP001576_188(CP001576|pid:none) Micromonas sp. RCC299 chromosome...    83   2e-14
AE016819_506(AE016819|pid:none) Ashbya gossypii (= Eremothecium ...    80   2e-13
(Q8AY73) RecName: Full=Exportin-2;          Short=Exp2; AltName:...    78   5e-13
AK039534_1(AK039534|pid:none) Mus musculus adult male spinal cor...    78   7e-13
AB027856_1(AB027856|pid:none) Schizosaccharomyces pombe gene for...    77   9e-13
AK053498_1(AK053498|pid:none) Mus musculus 0 day neonate eyeball...    77   1e-12
(Q7SZC2) RecName: Full=Exportin-2;          Short=Exp2; AltName:...    76   2e-12
(P55060) RecName: Full=Exportin-2;          Short=Exp2; AltName:...    76   3e-12
protein update 2009. 6.27
PSORT 

 psg: 0.85  gvh: 0.39  alm: 0.44  top: 0.43  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHB353 is nuc
5' end seq. ID VHB353F
5' end seq. 
>VHB353F.Seq
ACTGTTGGCCTACTGGNAATAATAATAGCTGAAGCAATAGTATCTCATTTAAGTAATAAA
AAAAAATGGATCCAATTATTCAAACCATTCAATTATTCCAACATACTCTTCATCATGACG
CTAATGTCATTAAAGCTGCCGAAGCTCAACTTCAACAAATTAAAGTAACTGATGGATATT
CAAGAATTTTATTAAAAATTTTAGCATCAAATGAAGTTGATATTTCAATTAGACAAGGTG
TTTCAATTTTTTTAAAGAATATGATCATTACAAAATGGAGAGGTGCTGAAGATGAATCAC
CAATCACTCAAGAGGATGCAGAATTCATTAAAGAGAATCTCATTGATCTTTTAGTTCATT
CACATCATTTAGTTCAAAATCAAATTGAAGCAATGATTGAAATTATTGCAAATCGTGATT
TCCCTGAAAAATGGACTTCATTATTACCAAAATCAATTCAATATATTAATACTCAAGATG
TTAAATTAATTTTAGCTGGTTTAACTTCAATTCAATTAGGTATTAAAAGATTTAGATATG
TAACAATGGGTGATAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 573
3' end seq. ID VHB353Z
3' end seq. 
>VHB353Z.Seq
NNNNNNNNNNATGGTTTAATAGAATTAAACATTTCCAAAGATTCTATGATAAAAAGATCT
CAGTTTTAGCATTCTCATCATTACTTACCCTTAATCCAAGTCCAAATTTCGTTAAATTTG
GTACATCTTTAATCTTGGAGAAAATGTTACAATTCACCAAAGATATGTTATCCATTGAAA
AGGAATTAGATAAACAAGAGGCTGAACGTGAACAAAAAATTAAAGATGGTACACTTAAAC
CAGAGGAAGAAGAATTCATTGATGAAAATGATGAAGAAGATTATTTTGATAATCATAAAT
TTGAATTTGAATTCACTGAAATTCCAGATAATCAAGATTGTCAACATGATGATGAAGGTG
AAGTATTCTTGGATGATATTGAAAAAGCAACTGAATACTTTGAAAATGGTGGTGATTTAG
GTGAAGATGAAGGTGATAACTTTGATGATCAAAACGACGATGATGATCAAGATTCTGAAG
AAGATCTCTTTGAAGATGAAGATACTCCAGACTTTGAAACCCCAATTGATGAAGTTGATG
GTTTCGAATTTATGATTAATTCAATTCAAAATTTCTTCCAAATTAATCCAACTTGTATTC
AACAAATCTCGAAAAACAACAAGAAAAAATTAAAAAATAT
Length of 3' end seq. 640
Connected seq. ID VHB353P
Connected seq. 
>VHB353P.Seq
ACTGTTGGCCTACTGGNAATAATAATAGCTGAAGCAATAGTATCTCATTTAAGTAATAAA
AAAAAATGGATCCAATTATTCAAACCATTCAATTATTCCAACATACTCTTCATCATGACG
CTAATGTCATTAAAGCTGCCGAAGCTCAACTTCAACAAATTAAAGTAACTGATGGATATT
CAAGAATTTTATTAAAAATTTTAGCATCAAATGAAGTTGATATTTCAATTAGACAAGGTG
TTTCAATTTTTTTAAAGAATATGATCATTACAAAATGGAGAGGTGCTGAAGATGAATCAC
CAATCACTCAAGAGGATGCAGAATTCATTAAAGAGAATCTCATTGATCTTTTAGTTCATT
CACATCATTTAGTTCAAAATCAAATTGAAGCAATGATTGAAATTATTGCAAATCGTGATT
TCCCTGAAAAATGGACTTCATTATTACCAAAATCAATTCAATATATTAATACTCAAGATG
TTAAATTAATTTTAGCTGGTTTAACTTCAATTCAATTAGGTATTAAAAGATTTAGATATG
TAACAATGGGTGATAAAAAAAAA----------ATGGTTTAATAGAATTAAACATTTCCA
AAGATTCTATGATAAAAAGATCTCAGTTTTAGCATTCTCATCATTACTTACCCTTAATCC
AAGTCCAAATTTCGTTAAATTTGGTACATCTTTAATCTTGGAGAAAATGTTACAATTCAC
CAAAGATATGTTATCCATTGAAAAGGAATTAGATAAACAAGAGGCTGAACGTGAACAAAA
AATTAAAGATGGTACACTTAAACCAGAGGAAGAAGAATTCATTGATGAAAATGATGAAGA
AGATTATTTTGATAATCATAAATTTGAATTTGAATTCACTGAAATTCCAGATAATCAAGA
TTGTCAACATGATGATGAAGGTGAAGTATTCTTGGATGATATTGAAAAAGCAACTGAATA
CTTTGAAAATGGTGGTGATTTAGGTGAAGATGAAGGTGATAACTTTGATGATCAAAACGA
CGATGATGATCAAGATTCTGAAGAAGATCTCTTTGAAGATGAAGATACTCCAGACTTTGA
AACCCCAATTGATGAAGTTGATGGTTTCGAATTTATGATTAATTCAATTCAAAATTTCTT
CCAAATTAATCCAACTTGTATTCAACAAATCTCGAAAAACAACAAGAAAAAATTAAAAAA
TAT
Length of connected seq. 1193
Full length Seq ID -
Full length Seq. -
Length of full length seq. -