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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB350
Library VH
(Link to library)
Clone ID VHB350
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15304-1
Original site URL
Representative seq. ID VHB350P
(Link to Original site)
Representative DNA sequence 
>VHB350 (VHB350Q) /CSM/VH/VHB3-C/VHB350Q.Seq.d/
AAATTTATTTCTCTACAAAGTGATTTCTTTATATGTGTTGATTGGCACATAGAAATATCT
ATTTAGAAAATATAAAATGGCTGAAAGACAATCAGCATCATCACCAACTCCTTCTTCACC
ACCACAACAACAACAACAAACACCTCAACAACCACCACAATATTTACTAAATTCAAAAAA
GAATTTTAAAGTAAAAGTATTAAATGGTAGAGAAGTTGAAAGACACCCATTAAATTCAAT
TACAAAAACAGAGGATACAGGTAAACCAAAACAATCATCATTATCATCAAATGCAAGTTC
ACTTCAATCAGCAGCAGCAGCAGCATCATCATCAACAGCAACAACAGATATTGATCCATT
AAACAACAATAATAATAATAATACAGATATTGATCCATTAAATAATCCATTAGAAAAAAA
ACATGATCCACTTTCAGAAACAATTGCAACAATGGGTGGATTAAAATTAATAAAAGAAAA
TCATTTAACAAATTATGTTGATGAGAATTTTATGCCATGGGATACATTGAAACCATCGAT
TCTTCAACAATATACCTCTGATGATTCAAATCCAATTCAAGTTTCTTTTATGTCAACTGG
TACAAGTGGTAAAXXXXXXXXXXTATTGCTTCATTACAATTCCATCAATTGATGATATCT
TTTTAAAGATGAATCTTTATTTAGTCTCTTCATCTGTGGCTTTACAAAATCAAGCACTCT
CTCAAGCCGATGCTTTATTAAAGGCTGCCATCACTTTTATTCAAGAAATCCCTCCAATCT
TGGAGTTTAAACAAGTTAAATCAACTGAAGATTGGACAATCTCTTATGTCTCTGATTTTA
TCTCACTTTTAGTTGTAACTCCTGGTCATCCTGAAAGTGGTCCTTTCTATTTAGTAAAGG
CTCTTTATAAAGTCATTAAAGAATATCAATGGGAATCAAGTTCAACTGCTAAATCAAAAT
TATTTATTCAATTATTATTACTTTGTTCTTCTTGGGCACAAACTTCATTACCATATCATA
TTGAAAAAGTTGAATCAAATGATCAATTATTTACAGAAGATCCAGAGTTTAATACAGAAT
TAACAGAATTTTATAATTCATTAATTAAAGAAATTTTATATGATTTAAATCTTTTGAAAG
ATGAACCAGATAATTTAACACAGAAAAAAGTTGGTATCATTTGTATTGATTTAATAAATG
CATTATTAAATGTTGGTGAATTAAATAGTAAGACAGCATCATTAATTTTCAATTTATATA
ATATGGCAAAGAAAATTATACCATCAACCTGTTTCAATGAAATTACATATTTAAAGAATA
CTCTTGCTTTTATTGGAACTNCTTGAATCTAAAATGGGTCAGGATATTTTTAATAAACTT
TCACAACAACAATAAGAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
nlflykvislyvligt*KYLFRKYKMAERQSASSPTPSSPPQQQQQTPQQPPQYLLNSKK
NFKVKVLNGREVERHPLNSITKTEDTGKPKQSSLSSNASSLQSAAAAASSSTATTDIDPL
NNNNNNNTDIDPLNNPLEKKHDPLSETIATMGGLKLIKENHLTNYVDENFMPWDTLKPSI
LQQYTSDDSNPIQVSFMSTGTSGK---

---YCFITIPSIDDIFLKMNLYLVSSSVALQNQALSQADALLKAAITFIQEIPPILEFKQ
VKSTEDWTISYVSDFISLLVVTPGHPESGPFYLVKALYKVIKEYQWESSSTAKSKLFIQL
LLLCSSWAQTSLPYHIEKVESNDQLFTEDPEFNTELTEFYNSLIKEILYDLNLLKDEPDN
LTQKKVGIICIDLINALLNVGELNSKTASLIFNLYNMAKKIIPSTCFNEITYLKNTLAFI
GTX*i*ngsgyf**tftttir
Translated Amino Acid sequence (All Frames) 
Frame A:
kfislqsdfficvdwhieisi*ki*ng*ktisiitnsfftttttttntsttttiftkfkk
ef*sksikw*rs*ktpikfnyknrgyr*tktiiiiikckftsisssssiiinsnnry*si
kqq****yry*sik*sirkkt*stfrnncnngwikinkrksfnklc**efyamgyietid
sstiyl**fksnssffyvnwykw*---

---YCFITIPSIDDIFLKMNLYLVSSSVALQNQALSQADALLKAAITFIQEIPPILEFKQ
VKSTEDWTISYVSDFISLLVVTPGHPESGPFYLVKALYKVIKEYQWESSSTAKSKLFIQL
LLLCSSWAQTSLPYHIEKVESNDQLFTEDPEFNTELTEFYNSLIKEILYDLNLLKDEPDN
LTQKKVGIICIDLINALLNVGELNSKTASLIFNLYNMAKKIIPSTCFNEITYLKNTLAFI
GTX*i*ngsgyf**tftttir

Frame B:
nlflykvislyvligt*KYLFRKYKMAERQSASSPTPSSPPQQQQQTPQQPPQYLLNSKK
NFKVKVLNGREVERHPLNSITKTEDTGKPKQSSLSSNASSLQSAAAAASSSTATTDIDPL
NNNNNNNTDIDPLNNPLEKKHDPLSETIATMGGLKLIKENHLTNYVDENFMPWDTLKPSI
LQQYTSDDSNPIQVSFMSTGTSGK---

---iaslqfhqlmisf*r*ifi*slhlwlykikhslkpmly*rlpsllfkkslqswslnk
lnqlkigqslmslilshf*l*llvilkvvlsi**rlfikslkningnqvqllnqnylfny
yyfvllghklhyhiilkklnqminylqkiqsliqn*qnfiih*lkkfymi*if*kmnqii
*hrkklvsfvli**mhy*mlvn*ivrqhh*fsiyiiwqrklyhqpvsmklhi*rilllll
elleskmgqdifnklsqqq*e

Frame C:
iyfstk*flymc*lahrniylenikwlkdnqhhhqlllhhhnnnnkhlnnhhniy*iqkr
ilk*ky*mveklkdth*iqlqkqriqvnqnnhhyhqmqvhfnqqqqqhhhqqqqqilih*
ttiiiiiqilih*iih*kknmihfqkqlqqwvd*n**kkii*qimlmrilchgih*nhrf
fnniplmiqiqfkfllcqlvqvv---

---llhynsin**ylfkdeslfslficgftksstlssrcfikgchhfysrnpsnlgv*ts
*in*rldnllcl*fyltfscnswss*kwsflfskgsl*sh*rismgikfnc*ikiiysii
itlfflgtnfitisy*ks*ik*siiyrrsrv*yrinril*fin*rnfi*fksfer*tr*f
ntekswyhly*fnkciikcw*ik**dsiinfqfi*ygkenytinlfq*nyifkeyscfyw
nxlnlkwvriflinfhnnnkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB350 (VHB350Q) /CSM/VH/VHB3-C/VHB350Q.Seq.d/                       2016   0.0
VHB123 (VHB123Q) /CSM/VH/VHB1-A/VHB123Q.Seq.d/                       1532   0.0
VHB873 (VHB873Q) /CSM/VH/VHB8-D/VHB873Q.Seq.d/                       1524   0.0
AHK310 (AHK310Q) /CSM/AH/AHK3-A/AHK310Q.Seq.d/                       1524   0.0
VHO612 (VHO612Q) /CSM/VH/VHO6-A/VHO612Q.Seq.d/                       1522   0.0
VHI487 (VHI487Q) /CSM/VH/VHI4-D/VHI487Q.Seq.d/                       1522   0.0
VHH623 (VHH623Q) /CSM/VH/VHH6-A/VHH623Q.Seq.d/                       1522   0.0
VHB181 (VHB181Q) /CSM/VH/VHB1-D/VHB181Q.Seq.d/                       1522   0.0
AHO186 (AHO186Q) /CSM/AH/AHO1-D/AHO186Q.Seq.d/                       1516   0.0
VHH857 (VHH857Q) /CSM/VH/VHH8-C/VHH857Q.Seq.d/                       1515   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC005308|AC005308.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING INPROGRESS ***.    56   7e-05  1
AC005506|AC005506.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    44   0.002  6
AC006279|AC006279.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    44   0.003  5
AC146263|AC146263.2 Pan troglodytes BAC clone RP43-28O1 from chromosome 7, complete sequence.    44   0.003  2
AC005139|AC005139.3 Plasmodium falciparum chromosome 12, WORKING DRAFT SEQUENCE, 5 unordered pieces.    44   0.004  8
AC004688|AC004688.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    44   0.008  5
AC004153|AC004153.7 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    44   0.012  7
AC006281|AC006281.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    44   0.013  7
AC099604|AC099604.4 Mus musculus chromosome 1, clone RP23-335B22, complete sequence.    40   0.013  5
AC012675|AC012675.5 Drosophila melanogaster, chromosome 2L, region 27C-27C, BAC clone BACR10E07, complete sequence.    48   0.016  1
dna update 2007. 4. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC007154_1(BC007154|pid:none) Mus musculus RIKEN cDNA 9030624J02...   155   3e-36
BC043674_1(BC043674|pid:none) Mus musculus RIKEN cDNA 9030624J02...   155   3e-36
(Q9H0L7) RecName: Full=UPF0505 protein C16orf62; AltName: Full=E...   154   6e-36
AL136744_1(AL136744|pid:none) Homo sapiens mRNA; cDNA DKFZp434B0...   154   6e-36
AK024693_1(AK024693|pid:none) Homo sapiens cDNA: FLJ21040 fis, c...   154   6e-36
AF461052_1(AF461052|pid:none) Homo sapiens esophageal cancer ass...   154   6e-36
AK095078_1(AK095078|pid:none) Homo sapiens cDNA FLJ37759 fis, cl...   154   6e-36
(Q5R8N4) RecName: Full=UPF0505 protein C16orf62 homolog;  &CR859...   154   7e-36
AK050302_1(AK050302|pid:none) Mus musculus adult male liver tumo...   154   1e-35
(Q9VHM2) RecName: Full=UPF0505 protein CG8202;  &AE014297_821(AE...   120   2e-25
protein update 2009. 6.27
PSORT 

 psg: 0.63  gvh: 0.27  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.45  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: mitochondrial
	 16.0 %: cytoplasmic
	 16.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane

>> prediction for VHB350 is mit
5' end seq. ID VHB350F
5' end seq. 
>VHB350F.Seq
AAATTTATTTCTCTACAAAGTGATTTCTTTATATGTGTTGATTGGCACATAGAAATATCT
ATTTAGAAAATATAAAATGGCTGAAAGACAATCAGCATCATCACCAACTCCTTCTTCACC
ACCACAACAACAACAACAAACACCTCAACAACCACCACAATATTTACTAAATTCAAAAAA
GAATTTTAAAGTAAAAGTATTAAATGGTAGAGAAGTTGAAAGACACCCATTAAATTCAAT
TACAAAAACAGAGGATACAGGTAAACCAAAACAATCATCATTATCATCAAATGCAAGTTC
ACTTCAATCAGCAGCAGCAGCAGCATCATCATCAACAGCAACAACAGATATTGATCCATT
AAACAACAATAATAATAATAATACAGATATTGATCCATTAAATAATCCATTAGAAAAAAA
ACATGATCCACTTTCAGAAACAATTGCAACAATGGGTGGATTAAAATTAATAAAAGAAAA
TCATTTAACAAATTATGTTGATGAGAATTTTATGCCATGGGATACATTGAAACCATCGAT
TCTTCAACAATATACCTCTGATGATTCAAATCCAATTCAAGTTTCTTTTATGTCAACTGG
TACAAGTGGTAAANNNNNNNNNN
Length of 5' end seq. 623
3' end seq. ID VHB350Z
3' end seq. 
>VHB350Z.Seq
NNNNNNNNNNTATTGCTTCATTACAATTCCATCAATTGATGATATCTTTTTAAAGATGAA
TCTTTATTTAGTCTCTTCATCTGTGGCTTTACAAAATCAAGCACTCTCTCAAGCCGATGC
TTTATTAAAGGCTGCCATCACTTTTATTCAAGAAATCCCTCCAATCTTGGAGTTTAAACA
AGTTAAATCAACTGAAGATTGGACAATCTCTTATGTCTCTGATTTTATCTCACTTTTAGT
TGTAACTCCTGGTCATCCTGAAAGTGGTCCTTTCTATTTAGTAAAGGCTCTTTATAAAGT
CATTAAAGAATATCAATGGGAATCAAGTTCAACTGCTAAATCAAAATTATTTATTCAATT
ATTATTACTTTGTTCTTCTTGGGCACAAACTTCATTACCATATCATATTGAAAAAGTTGA
ATCAAATGATCAATTATTTACAGAAGATCCAGAGTTTAATACAGAATTAACAGAATTTTA
TAATTCATTAATTAAAGAAATTTTATATGATTTAAATCTTTTGAAAGATGAACCAGATAA
TTTAACACAGAAAAAAGTTGGTATCATTTGTATTGATTTAATAAATGCATTATTAAATGT
TGGTGAATTAAATAGTAAGACAGCATCATTAATTTTCAATTTATATAATATGGCAAAGAA
AATTATACCATCAACCTGTTTCAATGAAATTACATATTTAAAGAATACTCTTGCTTTTAT
TGGAACTNCTTGAATCTAAAATGGGTCAGGATATTTTTAATAAACTTTCACAACAACAAT
AAGAAA
Length of 3' end seq. 786
Connected seq. ID VHB350P
Connected seq. 
>VHB350P.Seq
AAATTTATTTCTCTACAAAGTGATTTCTTTATATGTGTTGATTGGCACATAGAAATATCT
ATTTAGAAAATATAAAATGGCTGAAAGACAATCAGCATCATCACCAACTCCTTCTTCACC
ACCACAACAACAACAACAAACACCTCAACAACCACCACAATATTTACTAAATTCAAAAAA
GAATTTTAAAGTAAAAGTATTAAATGGTAGAGAAGTTGAAAGACACCCATTAAATTCAAT
TACAAAAACAGAGGATACAGGTAAACCAAAACAATCATCATTATCATCAAATGCAAGTTC
ACTTCAATCAGCAGCAGCAGCAGCATCATCATCAACAGCAACAACAGATATTGATCCATT
AAACAACAATAATAATAATAATACAGATATTGATCCATTAAATAATCCATTAGAAAAAAA
ACATGATCCACTTTCAGAAACAATTGCAACAATGGGTGGATTAAAATTAATAAAAGAAAA
TCATTTAACAAATTATGTTGATGAGAATTTTATGCCATGGGATACATTGAAACCATCGAT
TCTTCAACAATATACCTCTGATGATTCAAATCCAATTCAAGTTTCTTTTATGTCAACTGG
TACAAGTGGTAAA----------TATTGCTTCATTACAATTCCATCAATTGATGATATCT
TTTTAAAGATGAATCTTTATTTAGTCTCTTCATCTGTGGCTTTACAAAATCAAGCACTCT
CTCAAGCCGATGCTTTATTAAAGGCTGCCATCACTTTTATTCAAGAAATCCCTCCAATCT
TGGAGTTTAAACAAGTTAAATCAACTGAAGATTGGACAATCTCTTATGTCTCTGATTTTA
TCTCACTTTTAGTTGTAACTCCTGGTCATCCTGAAAGTGGTCCTTTCTATTTAGTAAAGG
CTCTTTATAAAGTCATTAAAGAATATCAATGGGAATCAAGTTCAACTGCTAAATCAAAAT
TATTTATTCAATTATTATTACTTTGTTCTTCTTGGGCACAAACTTCATTACCATATCATA
TTGAAAAAGTTGAATCAAATGATCAATTATTTACAGAAGATCCAGAGTTTAATACAGAAT
TAACAGAATTTTATAATTCATTAATTAAAGAAATTTTATATGATTTAAATCTTTTGAAAG
ATGAACCAGATAATTTAACACAGAAAAAAGTTGGTATCATTTGTATTGATTTAATAAATG
CATTATTAAATGTTGGTGAATTAAATAGTAAGACAGCATCATTAATTTTCAATTTATATA
ATATGGCAAAGAAAATTATACCATCAACCTGTTTCAATGAAATTACATATTTAAAGAATA
CTCTTGCTTTTATTGGAACTNCTTGAATCTAAAATGGGTCAGGATATTTTTAATAAACTT
TCACAACAACAATAAGAAA
Length of connected seq. 1389
Full length Seq ID -
Full length Seq. -
Length of full length seq. -