>VHB327 (VHB327Q) /CSM/VH/VHB3-B/VHB327Q.Seq.d/
ACAAAGGTCCATTTTAAAAATGGGTTTAGCAGGAGATTCACCATATGATTTTGGAGAAAT
TAAACCAGATGATTTAGATAAGTCAATTACAAAATTTCAAAATATTTTATCAAATTGTAT
TAAAGATTTAGAAACTGATGGAGAAGAAATAATAAAATTAGTGAAAACATTAATTTTAAC
TCAATCACCAGGTGAAAGAACTACAGAATTCATAGAGGAATATTTGAAAAGATTAGCAAT
CTAAGTAATACGGAAGCATTGAATGTATCAAGAGTGTTATCACATCTATTGAATTTAATT
AATGTTGCAGAACAACATCATTTGGTTAGATCAGTTAGAGAATCATTCCTCAACAATGAA
GATGATTTAAAGTATTCATGTGAGAATACCATTCAACAATTATTAAATCAAGGTCTTGAC
AAGGAGAGTGTTTACAATGCATTAATTCAACAAAAGATCGAACTTGTTTTAACGGCACAT
CCAACACAAATTATGAGACGTACTATAATTTCCAAGAATTCGGCAATGGGTGAAGCATTA
TCGTCATTGGATAATCAGCAATTGACAATGTTTGAACGTGAAGATTATGAAAAGAGATTG
ATTAGAGAAGTTGGTGGATCATGGTTAACCGATGAAGTTAGXXXXXXXXXXCCTTAACAT
TGGTTCTCGTCCTGCCAAAAGAATAGCCGCAAAATCATTTGGATTGGAACATTTACGTGC
TATTCCTTGGGTATTCAGTTTTTCACAAAATAGATTAAATTTACCAGTTTGGTTAGGTAT
TGAAGATGCAATTTTAGAGGCAAAAACAAAAGGATGGGGATCTGATATCAATGAAATGTA
CAAAGAATGGCCATTCTTTTCATCCACTATTGATTTGGTAGAGATGGTACTCTTGAAAAC
TGATCCTCAAATTTCATTGCGTTACAATCAAATGTTAGTACCAGCTCAAGTTCAACCTTT
AGGTATTGAAATGATTGATGGACTCACAAAAACTACAAATGCAATCTTGGAATTAACAAA
ACATAAAACTCTTCAACAAGATAATAAAATCCTTCAACATTTTGTTTCAATTCGTAGAAC
CTTTATGGATCCAATCAATTATATTCAAGTTGAAACCCTTAAAAGACTTCGTTCAACTCA
AAAACCAGATGGAACAAATGATGATGCTGATCCAATATTAATTGATACTTTAATTATTAC
TTTTAATGGTATTTCTGCTGGTATGAAAAATACTGGTTAAATTATAATTATAAAATTTTA
ATAATCAATTAATTAATAATAATAAAAATAATAATAATAAAAATAATAATAATAGTTCTC
CATTATTTCAAAGATGCGAAAAATAAATAAATTAAAAANNAAAA

tkvhfkngfsrrfti*fwrn*tr*fr*vnykiskyfikly*rfrn*wrrnnkiseninfn
sitr*KNYRIHRGIFEKISNLSNTEALNVSRVLSHLLNLINVAEQHHLVRSVRESFLNNE
DDLKYSCENTIQQLLNQGLDKESVYNALIQQKIELVLTAHPTQIMRRTIISKNSAMGEAL
SSLDNQQLTMFEREDYEKRLIREVGGSWLTDEV---

---LNIGSRPAKRIAAKSFGLEHLRAIPWVFSFSQNRLNLPVWLGIEDAILEAKTKGWGS
DINEMYKEWPFFSSTIDLVEMVLLKTDPQISLRYNQMLVPAQVQPLGIEMIDGLTKTTNA
ILELTKHKTLQQDNKILQHFVSIRRTFMDPINYIQVETLKRLRSTQKPDGTNDDADPILI
DTLIITFNGISAGMKNTG*iiiikf**sin****k****k****fsiiskmrkinklkxk

Frame A:
tkvhfkngfsrrfti*fwrn*tr*fr*vnykiskyfikly*rfrn*wrrnnkiseninfn
sitr*KNYRIHRGIFEKISNLSNTEALNVSRVLSHLLNLINVAEQHHLVRSVRESFLNNE
DDLKYSCENTIQQLLNQGLDKESVYNALIQQKIELVLTAHPTQIMRRTIISKNSAMGEAL
SSLDNQQLTMFEREDYEKRLIREVGGSWLTDEV---

---p*hwfsscqknsrkiiwigtftcyslgiqfftk*ikftslvry*rcnfrgknkrmgi
*yq*nvqrmailfihy*fgrdgtlen*ssnfialqsnvstsssstfry*nd*wthknykc
nlginkt*nsstr**npstfcfns*nlygsnqlyss*np*ktsfnsktrwnk**c*snin
*yfnyyf*wyfcwyekywlnynykiliin*liiikiiiikiiiivlhyfkdaknk*ikxx

Frame B:
qrsilkmglagdspydfgeikpddldksitkfqnilsncikdletdgeeiiklvktlilt
qspgerttefieeylkrlai*virkh*myqecyhiy*i*lmlqnniiwldqlenhsstmk
mi*sihvripfnny*ikvltrrvftmh*fnkrsnlf*rhiqhkl*dvl*fprirqwvkhy
rhwiisn*qclnvkimkrd*leklvdhg*pmkl---

---LNIGSRPAKRIAAKSFGLEHLRAIPWVFSFSQNRLNLPVWLGIEDAILEAKTKGWGS
DINEMYKEWPFFSSTIDLVEMVLLKTDPQISLRYNQMLVPAQVQPLGIEMIDGLTKTTNA
ILELTKHKTLQQDNKILQHFVSIRRTFMDPINYIQVETLKRLRSTQKPDGTNDDADPILI
DTLIITFNGISAGMKNTG*iiiikf**sin****k****k****fsiiskmrkinklkxk

Frame C:
kgpf*kwv*qeihhmileklnqmi*isqlqnfkifyqivlki*klmekk**n**kh*f*l
nhqvkelqns*rni*kd*qsk*ygsieciksvitsiefn*ccrttsfg*is*riipqq*r
*fkvfm*eyhstiiksrs*qgeclqcinstkdrtcfngtsntnyetyynfqefgng*sii
vig*saidnv*t*rl*keid*rswwimvnr*s*---

---ltlvlvlpke*pqnhldwniyvlflgysvfhkid*iyqfg*vlkmqf*rqkqkdgdl
ismkctknghsfhplliw*rwys*klilkfhcvtikc*yqlkfnl*vlk*lmdsqklqmq
swn*qniklfnkiiksfnilfqfveplwiqsiifklkplkdfvqlknqmeqmmmliqy*l
il*llllmvfllv*kilvkl*l*nfnnqlinnnknnnnknnnnssplfqrcek*in*kxk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB327 (VHB327Q) /CSM/VH/VHB3-B/VHB327Q.Seq.d/                       2369   0.0
VHK432 (VHK432Q) /CSM/VH/VHK4-B/VHK432Q.Seq.d/                       1249   0.0
AHI617 (AHI617Q) /CSM/AH/AHI6-A/AHI617Q.Seq.d/                       1249   0.0
AHI865 (AHI865Q) /CSM/AH/AHI8-C/AHI865Q.Seq.d/                       1241   0.0
VHO704 (VHO704Q) /CSM/VH/VHO7-A/VHO704Q.Seq.d/                       1227   0.0
VHI520 (VHI520Q) /CSM/VH/VHI5-A/VHI520Q.Seq.d/                       1227   0.0
AHQ519 (AHQ519Q) /CSM/AH/AHQ5-A/AHQ519Q.Seq.d/                       1223   0.0
VHL105 (VHL105Q) /CSM/VH/VHL1-A/VHL105Q.Seq.d/                       1221   0.0
VHJ139 (VHJ139Q) /CSM/VH/VHJ1-B/VHJ139Q.Seq.d/                       1219   0.0
SHF259 (SHF259Q) /CSM/SH/SHF2-C/SHF259Q.Seq.d/                       1217   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

DQ538352|DQ538352.1 Bienertia sinuspersici phosphoenolpyruvate carboxylase mRNA, complete cds.    48   2e-07  2
DQ538353|DQ538353.1 Suaeda aralocaspica phosphoenolpyruvate carboxylase mRNA, partial cds.    48   2e-07  2
DQ538354|DQ538354.1 Suaeda eltonica phosphoenolpyruvate carboxylase mRNA, partial cds.    40   4e-05  2
CJ768150|CJ768150.1 Ipomoea nil cDNA clone:jmsf51j06, 3' end.      46   2e-04  2
BJ573459|BJ573459.1 Ipomoea nil cDNA clone:jm23o22, 3' end, single read.    46   2e-04  2
BJ572016|BJ572016.1 Ipomoea nil cDNA clone:jm19g15, 3' end, single read.    46   2e-04  2
CO911440|CO911440.1 BJ03019B12 BJ03 Capsicum annuum cDNA 5', mRNA sequence.    56   0.003  1
E03014|E03014.1 DNA encoding phosphoenolpyruvic acid carboxylase(PEPC).    56   0.003  1
X59016|X59016.1 N.tabacum mRNA for phosphoenolpyruvate carboxylase.    56   0.003  1
AB078720|AB078720.3 Nicotiana sylvestris Nsppc3 gene for phosphoenolpyruvate carboxylase, partial cds.    56   0.003  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EF634318_1(EF634318|pid:none) Ricinus communis phosphoenolpyruva...   171   7e-41
(Q6R2V6) RecName: Full=Phosphoenolpyruvate carboxylase 2;       ...   170   1e-40
BT070413_1(BT070413|pid:none) Picea sitchensis clone WS02727_O18...   170   1e-40
FB830099_1(FB830099|pid:none) Sequence 49372 from Patent WO20080...   168   5e-40
AM424873_1(AM424873|pid:none) Vitis vinifera contig VV78X088960....   167   8e-40
AJ231278_1(AJ231278|pid:none) Anthoceros punctatus partial mRNA ...   167   1e-39
AC011914_12(AC011914|pid:none) Arabidopsis thaliana chromosome 1...   166   1e-39
AC008075_1(AC008075|pid:none) Arabidopsis thaliana chromosome 1 ...   166   1e-39
CP000544_2259(CP000544|pid:none) Halorhodospira halophila SL1, c...   153   2e-35
(A4VN44) RecName: Full=Phosphoenolpyruvate carboxylase;         ...   152   4e-35

 psg: 0.65  gvh: 0.51  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 24.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for VHB327 is cyt

>VHB327F.Seq
ACAAAGGTCCATTTTAAAAATGGGTTTAGCAGGAGATTCACCATATGATTTTGGAGAAAT
TAAACCAGATGATTTAGATAAGTCAATTACAAAATTTCAAAATATTTTATCAAATTGTAT
TAAAGATTTAGAAACTGATGGAGAAGAAATAATAAAATTAGTGAAAACATTAATTTTAAC
TCAATCACCAGGTGAAAGAACTACAGAATTCATAGAGGAATATTTGAAAAGATTAGCAAT
CTAAGTAATACGGAAGCATTGAATGTATCAAGAGTGTTATCACATCTATTGAATTTAATT
AATGTTGCAGAACAACATCATTTGGTTAGATCAGTTAGAGAATCATTCCTCAACAATGAA
GATGATTTAAAGTATTCATGTGAGAATACCATTCAACAATTATTAAATCAAGGTCTTGAC
AAGGAGAGTGTTTACAATGCATTAATTCAACAAAAGATCGAACTTGTTTTAACGGCACAT
CCAACACAAATTATGAGACGTACTATAATTTCCAAGAATTCGGCAATGGGTGAAGCATTA
TCGTCATTGGATAATCAGCAATTGACAATGTTTGAACGTGAAGATTATGAAAAGAGATTG
ATTAGAGAAGTTGGTGGATCATGGTTAACCGATGAAGTTAGNNNNNNNNNN

>VHB327Z.Seq
NNNNNNNNNNCCTTAACATTGGTTCTCGTCCTGCCAAAAGAATAGCCGCAAAATCATTTG
GATTGGAACATTTACGTGCTATTCCTTGGGTATTCAGTTTTTCACAAAATAGATTAAATT
TACCAGTTTGGTTAGGTATTGAAGATGCAATTTTAGAGGCAAAAACAAAAGGATGGGGAT
CTGATATCAATGAAATGTACAAAGAATGGCCATTCTTTTCATCCACTATTGATTTGGTAG
AGATGGTACTCTTGAAAACTGATCCTCAAATTTCATTGCGTTACAATCAAATGTTAGTAC
CAGCTCAAGTTCAACCTTTAGGTATTGAAATGATTGATGGACTCACAAAAACTACAAATG
CAATCTTGGAATTAACAAAACATAAAACTCTTCAACAAGATAATAAAATCCTTCAACATT
TTGTTTCAATTCGTAGAACCTTTATGGATCCAATCAATTATATTCAAGTTGAAACCCTTA
AAAGACTTCGTTCAACTCAAAAACCAGATGGAACAAATGATGATGCTGATCCAATATTAA
TTGATACTTTAATTATTACTTTTAATGGTATTTCTGCTGGTATGAAAAATACTGGTTAAA
TTATAATTATAAAATTTTAATAATCAATTAATTAATAATAATAAAAATAATAATAATAAA
AATAATAATAATAGTTCTCCATTATTTCAAAGATGCGAAAAATAAATAAATTAAAAANNA
AAA

>VHB327P.Seq
ACAAAGGTCCATTTTAAAAATGGGTTTAGCAGGAGATTCACCATATGATTTTGGAGAAAT
TAAACCAGATGATTTAGATAAGTCAATTACAAAATTTCAAAATATTTTATCAAATTGTAT
TAAAGATTTAGAAACTGATGGAGAAGAAATAATAAAATTAGTGAAAACATTAATTTTAAC
TCAATCACCAGGTGAAAGAACTACAGAATTCATAGAGGAATATTTGAAAAGATTAGCAAT
CTAAGTAATACGGAAGCATTGAATGTATCAAGAGTGTTATCACATCTATTGAATTTAATT
AATGTTGCAGAACAACATCATTTGGTTAGATCAGTTAGAGAATCATTCCTCAACAATGAA
GATGATTTAAAGTATTCATGTGAGAATACCATTCAACAATTATTAAATCAAGGTCTTGAC
AAGGAGAGTGTTTACAATGCATTAATTCAACAAAAGATCGAACTTGTTTTAACGGCACAT
CCAACACAAATTATGAGACGTACTATAATTTCCAAGAATTCGGCAATGGGTGAAGCATTA
TCGTCATTGGATAATCAGCAATTGACAATGTTTGAACGTGAAGATTATGAAAAGAGATTG
ATTAGAGAAGTTGGTGGATCATGGTTAACCGATGAAGTTAG----------CCTTAACAT
TGGTTCTCGTCCTGCCAAAAGAATAGCCGCAAAATCATTTGGATTGGAACATTTACGTGC
TATTCCTTGGGTATTCAGTTTTTCACAAAATAGATTAAATTTACCAGTTTGGTTAGGTAT
TGAAGATGCAATTTTAGAGGCAAAAACAAAAGGATGGGGATCTGATATCAATGAAATGTA
CAAAGAATGGCCATTCTTTTCATCCACTATTGATTTGGTAGAGATGGTACTCTTGAAAAC
TGATCCTCAAATTTCATTGCGTTACAATCAAATGTTAGTACCAGCTCAAGTTCAACCTTT
AGGTATTGAAATGATTGATGGACTCACAAAAACTACAAATGCAATCTTGGAATTAACAAA
ACATAAAACTCTTCAACAAGATAATAAAATCCTTCAACATTTTGTTTCAATTCGTAGAAC
CTTTATGGATCCAATCAATTATATTCAAGTTGAAACCCTTAAAAGACTTCGTTCAACTCA
AAAACCAGATGGAACAAATGATGATGCTGATCCAATATTAATTGATACTTTAATTATTAC
TTTTAATGGTATTTCTGCTGGTATGAAAAATACTGGTTAAATTATAATTATAAAATTTTA
ATAATCAATTAATTAATAATAATAAAAATAATAATAATAAAAATAATAATAATAGTTCTC
CATTATTTCAAAGATGCGAAAAATAAATAAATTAAAAANNAAAA