>VHB325 (VHB325Q) /CSM/VH/VHB3-B/VHB325Q.Seq.d/
ATTTTATTTTGATTTAAATAAATAAATATAAATAAAAAAAAAAGATGTTTAAAAAACCAG
CATCACAAGCACCTGGTGGTAAACCAGCGGCTCAACAACAACAACAAGCAAAACAACAAC
AACAACCACAACCATTAAAACCAATAGTATTATCACAAATTCCAAATATTTCAAGTAAAG
TTTTAAATTTAACATCGATTAGAGATCAAAGTAAATTAGAGTTAGGTGCAGCAATTAGTA
CACTCAAAGGTTCAAAAGCATTAGTTATGGATCCAAAATTAATTGGTTTATTAAATTTAT
TAGCTGATCCAACATTTTTATCTAATTGTGGAGCAGATAGAAGATATGAATTAAAACCAG
AGATTAATACAGATAGTAAGAATATAGTTTATATAGTTAGACCAGAAGTTAAATATATGC
ATTGGATAACTGAACATGTTAAACAACATGCAGAGAAAGGGTTAAAGAAAGAGTATTCAA
TTGTATTTGTACCAAGAGCAACCATTTTATGTCAACGTGTTTTAGAGGAACAGGGTGTGA
TGGGTAATTTTGTAATTAGTGATTTCCCATTGGATATTGTACCATTCGATGAGGATXXXX
XXXXXX

fili*inkyk*KKKMFKKPASQAPGGKPAAQQQQQAKQQQQPQPLKPIVLSQIPNISSKV
LNLTSIRDQSKLELGAAISTLKGSKALVMDPKLIGLLNLLADPTFLSNCGADRRYELKPE
INTDSKNIVYIVRPEVKYMHWITEHVKQHAEKGLKKEYSIVFVPRATILCQRVLEEQGVM
GNFVISDFPLDIVPFDE---

Frame A:
ilf*fk*ininkkkrclknqhhkhlvvnqrlnnnnkqnnnnnhnh*nq*yyhkfqifqvk
f*i*hrleikvn*s*vqqlvhskvqkh*lwiqn*lvy*iy*liqhfyliveqiedmn*nq
rliqivri*fi*ldqklnicig*lnmlnnmqrkg*rksiqlylyqeqpfyvnvf*rnrv*
wvil*lvishwilyhsmr---


Frame B:
fyfdlnk*i*ikkkdv*ktsitstww*tsgsttttsktttttttiktnsiitnskyfk*s
fkfnid*rsk*irvrcsn*ytqrfksisygskinwfikfis*snifi*lwsr*ki*iktr
d*yr**eyslys*trs*iyaldn*tc*ttcrervkervfncictksnhfmstcfrgtgcd
g*fcn**fpigyctir*g---


Frame C:
fili*inkyk*KKKMFKKPASQAPGGKPAAQQQQQAKQQQQPQPLKPIVLSQIPNISSKV
LNLTSIRDQSKLELGAAISTLKGSKALVMDPKLIGLLNLLADPTFLSNCGADRRYELKPE
INTDSKNIVYIVRPEVKYMHWITEHVKQHAEKGLKKEYSIVFVPRATILCQRVLEEQGVM
GNFVISDFPLDIVPFDE---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB325 (VHB325Q) /CSM/VH/VHB3-B/VHB325Q.Seq.d/                        920   0.0
VFL219 (VFL219Q) /CSM/VF/VFL2-A/VFL219Q.Seq.d/                        866   0.0
AFJ416 (AFJ416Q) /CSM/AF/AFJ4-A/AFJ416Q.Seq.d/                        773   0.0
VHK309 (VHK309Q) /CSM/VH/VHK3-A/VHK309Q.Seq.d/                        771   0.0
VHN724 (VHN724Q) /CSM/VH/VHN7-A/VHN724Q.Seq.d/                        529   e-149
VFI313 (VFI313Q) /CSM/VF/VFI3-A/VFI313Q.Seq.d/                        474   e-133
VHI138 (VHI138Q) /CSM/VH/VHI1-B/VHI138Q.Seq.d/                        371   e-102
SSE872 (SSE872Q) /CSM/SS/SSE8-C/SSE872Q.Seq.d/                         44   0.002
SSD305 (SSD305Q) /CSM/SS/SSD3-A/SSD305Q.Seq.d/                         44   0.002
VSJ263 (VSJ263Q) /CSM/VS/VSJ2-C/VSJ263Q.Seq.d/                         40   0.038

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117080|AC117080.2 Dictyostelium discoideum chromosome 2 map complement(821514-735598) strain AX4, complete sequence.    50   0.072  1
AC021429|AC021429.3 Homo sapiens chromosome 4 clone RP11-471K8 map 4, LOW-PASS SEQUENCE SAMPLING.    48   0.28   1
AC114741|AC114741.3 Homo sapiens BAC clone RP11-62N21 from 4, complete sequence.    48   0.28   1
CL585846|CL585846.1 OB__Ba0081L08.r OB__Ba Oryza brachyantha genomic clone OB__Ba0081L08 3', genomic survey sequence.    46   1.1    1
CR383669|CR383669.17 Zebrafish DNA sequence from clone DKEY-25O1 in linkage group 24.    46   1.1    1
AL732508|AL732508.13 Mouse DNA sequence from clone RP23-270A11 on chromosome 4.    46   1.1    1
AL121898|AL121898.23 Human DNA sequence from clone RP11-430K20 on chromosome 20. Contains the 5' end of the PLCB4 gene for phospholipase C beta 4, STSs, GSSs  and a CpG island.    46   1.1    1
BD439871|BD439871.1 Nucleotide Sequence of the Mycoplasma Genitalium Genome , Fragments Thereof, and Uses Thereof.    36   2.0    9
AR300198|AR300198.1 Sequence 1 from patent US 6537773.             36   2.0    9
L43967|L43967.2 Mycoplasma genitalium G37, complete genome.        36   2.0    9

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK067177_1(AK067177|pid:none) Oryza sativa Japonica Group cDNA c...   122   8e-27
EU961408_1(EU961408|pid:none) Zea mays clone 235200 vacuolar pro...   121   1e-26
(Q94KJ7) RecName: Full=Vacuolar protein sorting-associated prote...   116   4e-25
(Q63615) RecName: Full=Vacuolar protein sorting-associated prote...    91   3e-17
BT058867_1(BT058867|pid:none) Salmo salar clone ssal-rgf-509-048...    89   9e-17
AB271950_1(AB271950|pid:none) Sus scrofa VPS33A mRNA for vacuola...    88   2e-16
BC105478_1(BC105478|pid:none) Bos taurus vacuolar protein sortin...    88   2e-16
BC166051_1(BC166051|pid:none) Xenopus laevis hypothetical protei...    88   2e-16
CR762099_1(CR762099|pid:none) Xenopus tropicalis finished cDNA, ...    88   2e-16
CT978957_4(CT978957|pid:none) Zebrafish DNA sequence from clone ...    82   9e-15

 psg: 0.77  gvh: 0.37  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.44  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: mitochondrial
	 20.0 %: nuclear
	 12.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VHB325 is mit

>VHB325F.Seq
ATTTTATTTTGATTTAAATAAATAAATATAAATAAAAAAAAAAGATGTTTAAAAAACCAG
CATCACAAGCACCTGGTGGTAAACCAGCGGCTCAACAACAACAACAAGCAAAACAACAAC
AACAACCACAACCATTAAAACCAATAGTATTATCACAAATTCCAAATATTTCAAGTAAAG
TTTTAAATTTAACATCGATTAGAGATCAAAGTAAATTAGAGTTAGGTGCAGCAATTAGTA
CACTCAAAGGTTCAAAAGCATTAGTTATGGATCCAAAATTAATTGGTTTATTAAATTTAT
TAGCTGATCCAACATTTTTATCTAATTGTGGAGCAGATAGAAGATATGAATTAAAACCAG
AGATTAATACAGATAGTAAGAATATAGTTTATATAGTTAGACCAGAAGTTAAATATATGC
ATTGGATAACTGAACATGTTAAACAACATGCAGAGAAAGGGTTAAAGAAAGAGTATTCAA
TTGTATTTGTACCAAGAGCAACCATTTTATGTCAACGTGTTTTAGAGGAACAGGGTGTGA
TGGGTAATTTTGTAATTAGTGATTTCCCATTGGATATTGTACCATTCGATGAGGATNNNN
NNNNNN