>VHB312 (VHB312Q) /CSM/VH/VHB3-A/VHB312Q.Seq.d/
XXXXXXXXXXTGATAGTAACATAGAGGCTATCATTGCAACTCTTGAACGTTTTAAAATCA
ATGCAATTCTAATGTTTGGTGGATTCAATGGTTATTTGGGCATAGCAAAACTCTACGAAT
ATAGAGAGAAATATCAACAACTTAAACGTATCTCAATCATTGGTGCACNTGGTACCATTG
CAAACAACGTACCAGGTACAAATATCACATTGAATACACTAACCAAGGATTTGGAAATGT
TTGTTGAACGTTTCAAACGTGAACATAGAATTGATCTAATTATTAAGAGTGAACTTGCTA
GCAACACCTACTCAACTCATTTCATTTATTCACTCTTCAAAGAAGAAGGTAAACATTTAT
TCGATGTCCGTGAATCTATACTCGGTCATTTACAACAAGGTGGTACTCCTTCTGCAATTG
ATCGTATCTTTTCAACTCGTTTAATGAATCATTATTATCAATTCTTGGAAAACGATCTCA
AAGAACATGGTCATCTTCAAATGAATGGTTGTATTGGTTTCATTGATGGTGGTATTCATT
ACACTCCAATGCAAGAAATGATCGAAGAAATGTCTGATAAATTTAGACGTCCTCGTTCCC
AATGGTGGATGGATTTGGTTGAAACCTCTCAAAATATCTCTGTTTTCCCTTTAGATGACC
CTTCCTCTACTAATTTCGAAGGTTGTAATTCAAATTTATCGGAACAAGATAGACCTATTA
AATAA

---DSNIEAIIATLERFKINAILMFGGFNGYLGIAKLYEYREKYQQLKRISIIGAXGTIA
NNVPGTNITLNTLTKDLEMFVERFKREHRIDLIIKSELASNTYSTHFIYSLFKEEGKHLF
DVRESILGHLQQGGTPSAIDRIFSTRLMNHYYQFLENDLKEHGHLQMNGCIGFIDGGIHY
TPMQEMIEEMSDKFRRPRSQWWMDLVETSQNISVFPLDDPSSTNFEGCNSNLSEQDRPIK
*

Frame A:
---DSNIEAIIATLERFKINAILMFGGFNGYLGIAKLYEYREKYQQLKRISIIGAXGTIA
NNVPGTNITLNTLTKDLEMFVERFKREHRIDLIIKSELASNTYSTHFIYSLFKEEGKHLF
DVRESILGHLQQGGTPSAIDRIFSTRLMNHYYQFLENDLKEHGHLQMNGCIGFIDGGIHY
TPMQEMIEEMSDKFRRPRSQWWMDLVETSQNISVFPLDDPSSTNFEGCNSNLSEQDRPIK
*


Frame B:
---ivt*rlslqllnvlksmqf*clvdsmviwa*qnstnierninnlnvsqslvhxvplq
ttyqvqish*ih*priwkcllnvsnvnieli*llrvnllatptqlisfihsskkkvniys
msvnlysviynkvvlllqlivsfqlv**iiiinswktisknmvifk*mvvlvslmvvfit
lqckk*skkclinldvlvpnggwiwlkplkislfsl*mtlplliskvviqiyrnkidlln


Frame C:
---**hrgyhcns*tf*nqcnsnvwwiqwlfghsktlri*reistt*tylnhwctwyhck
qrtrykyhieytnqgfgnvc*tfqt*t*n*sny*e*tc*qhllnsfhlftlqrrr*tfir
cp*iytrsfttrwysfcn*sylfnsfnesllsilgkrsqrtwsssnewlywfh*wwyslh
snarndrrnv**i*tssfpmvdgfg*nlskylcfpfr*pfly*frrl*fkfigtr*ty*i

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB312 (VHB312Q) /CSM/VH/VHB3-A/VHB312Q.Seq.d/                       1411   0.0
VHB661 (VHB661Q) /CSM/VH/VHB6-C/VHB661Q.Seq.d/                       1292   0.0
VFH864 (VFH864Q) /CSM/VF/VFH8-C/VFH864Q.Seq.d/                       1247   0.0
CHP734 (CHP734Q) /CSM/CH/CHP7-B/CHP734Q.Seq.d/                       1179   0.0
CHN469 (CHN469Q) /CSM/CH/CHN4-C/CHN469Q.Seq.d/                       1144   0.0
CHP390 (CHP390Q) /CSM/CH/CHP3-D/CHP390Q.Seq.d/                       1047   0.0
SHA235 (SHA235Q) /CSM/SH/SHA2-B/SHA235Q.Seq.d/                       1029   0.0
AHH109 (AHH109Q) /CSM/AH/AHH1-A/AHH109Q.Seq.d/                       1027   0.0
VHJ854 (VHJ854Q) /CSM/VH/VHJ8-C/VHJ854Q.Seq.d/                       1023   0.0
VHJ475 (VHJ475Q) /CSM/VH/VHJ4-D/VHJ475Q.Seq.d/                       1023   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X94340|X94340.1 D.discoideum mRNA for 6-phosphofructo-1-kinase.  1023   0.0    2
X89039|X89039.1 D.discoideum mRNA for 6-phosphofructo-1-kinase.  1023   0.0    2
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.  1023   0.0    2
DX414156|DX414156.1 SOYC248TH LargeInsertSoybeanGenLibBuild4 Glycine max genomic clone H12A17:Build4MTP4H23, genomic survey sequence.    48   0.34   1
CE635221|CE635221.1 tigr-gss-dog-17000366743799 Dog Library Canis familiaris genomic, genomic survey sequence.    46   1.4    1
BZ298846|BZ298846.1 CG4616.f1 Candida glabrata Random Genomic Library Candida glabrata genomic clone CG4616, DNA sequence.    46   1.4    1
BZ297417|BZ297417.1 CG3420.r1 Candida glabrata Random Genomic Library Candida glabrata genomic clone CG3420, DNA sequence.    46   1.4    1
CR380955|CR380955.1 Candida glabrata strain CBS138 chromosome I complete sequence.    46   1.4    1
U97104|U97104.1 Bactrocera tryoni membrane transporter (white) gene, complete cds.    46   1.4    1
AM457870|AM457870.1 Vitis vinifera, whole genome shotgun sequence, contig VV78X115751.5, clone ENTAV 115.    40   1.5    2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P90521) RecName: Full=6-phosphofructokinase;          Short=Pho...   367   e-100
CP001337_2617(CP001337|pid:none) Chloroflexus aggregans DSM 9485...   116   6e-25
BC081652_1(BC081652|pid:none) Danio rerio phosphofructokinase, m...   114   3e-24
A53206(A53206;S03880)6-phosphofructokinase (EC 2.7.1.11) C - rab...   109   7e-23
(P47859) RecName: Full=6-phosphofructokinase type C;          EC...   109   7e-23
AB061205_1(AB061205|pid:none) Gallus gallus pfk mRNA for 6-phosp...   107   3e-22
BC133214_1(BC133214|pid:none) Xenopus laevis hypothetical protei...   107   5e-22
(Q01813) RecName: Full=6-phosphofructokinase type C;          EC...   107   5e-22
BC021698_1(BC021698|pid:none) Homo sapiens, Similar to phosphofr...   107   5e-22
AK092597_1(AK092597|pid:none) Homo sapiens cDNA FLJ35278 fis, cl...   106   6e-22

 psg: 0.75  gvh: 0.39  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.03  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHB312 is cyt

>VHB312Z.Seq
NNNNNNNNNNTGATAGTAACATAGAGGCTATCATTGCAACTCTTGAACGTTTTAAAATCA
ATGCAATTCTAATGTTTGGTGGATTCAATGGTTATTTGGGCATAGCAAAACTCTACGAAT
ATAGAGAGAAATATCAACAACTTAAACGTATCTCAATCATTGGTGCACNTGGTACCATTG
CAAACAACGTACCAGGTACAAATATCACATTGAATACACTAACCAAGGATTTGGAAATGT
TTGTTGAACGTTTCAAACGTGAACATAGAATTGATCTAATTATTAAGAGTGAACTTGCTA
GCAACACCTACTCAACTCATTTCATTTATTCACTCTTCAAAGAAGAAGGTAAACATTTAT
TCGATGTCCGTGAATCTATACTCGGTCATTTACAACAAGGTGGTACTCCTTCTGCAATTG
ATCGTATCTTTTCAACTCGTTTAATGAATCATTATTATCAATTCTTGGAAAACGATCTCA
AAGAACATGGTCATCTTCAAATGAATGGTTGTATTGGTTTCATTGATGGTGGTATTCATT
ACACTCCAATGCAAGAAATGATCGAAGAAATGTCTGATAAATTTAGACGTCCTCGTTCCC
AATGGTGGATGGATTTGGTTGAAACCTCTCAAAATATCTCTGTTTTCCCTTTAGATGACC
CTTCCTCTACTAATTTCGAAGGTTGTAATTCAAATTTATCGGAACAAGATAGACCTATTA
AATAA