>VHB310 (VHB310Q) /CSM/VH/VHB3-A/VHB310Q.Seq.d/
XXXXXXXXXXCTCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGCTGATAACC
ACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTG
CCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTT
TCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAG
GTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTG
GTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAA
CTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTA
TTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTT
CCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTG
ATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTT
CAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGA
GAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAA
TTTAAGTCGCACTTGAGTTTTCAAANATAGTAATCAAATCTAAAAAAAA

---SSKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSF
VGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPT
LLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTAD
LRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti
*valefsxivikskk

Frame A:
---SSKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSF
VGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPT
LLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTAD
LRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti
*valefsxivikskk


Frame B:
---hpkmiqklvltislittngikmmp*tfglsvqkvtvpisssmspkvfni*mklkils
lvlsngppkkvlfvmkt*evsvsifmmspstlmpstevvvkssqllvvysmlln*lppql
s*nqst**kllpqktllvvftlysivvevlllvknvvsvphyslskliyqy*npsvspli
yvptlpvklshnvssiiglqlvlstkirkplksl*pperekv*pqksqiltnstknskqf
kshlsfqx**snlkk


Frame C:
---iqr*skssc*lsr**prmg*k*chehlvfrsrr*rcqsprqchqrcsife*n*rffr
wcfpmghqrrccl**kherypfqsl*chpph*chpqrwwsnhpncssctlcc*inclpns
lrtnllsrnycprkrywwyllctqss*rycyw*rtsyrfpiilcqssftsirilrfhr*f
tfphcrssfptmclrslgfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnl
srt*vfkxsnqi*kk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ186 (VHQ186Q) /CSM/VH/VHQ1-D/VHQ186Q.Seq.d/                       1483   0.0
VHP454 (VHP454Q) /CSM/VH/VHP4-C/VHP454Q.Seq.d/                       1483   0.0
VHO819 (VHO819Q) /CSM/VH/VHO8-A/VHO819Q.Seq.d/                       1483   0.0
VHD209 (VHD209Q) /CSM/VH/VHD2-A/VHD209Q.Seq.d/                       1483   0.0
VHD127 (VHD127Q) /CSM/VH/VHD1-B/VHD127Q.Seq.d/                       1483   0.0
VHC525 (VHC525Q) /CSM/VH/VHC5-B/VHC525Q.Seq.d/                       1483   0.0
VHB840 (VHB840Q) /CSM/VH/VHB8-B/VHB840Q.Seq.d/                       1483   0.0
VHB674 (VHB674Q) /CSM/VH/VHB6-D/VHB674Q.Seq.d/                       1483   0.0
VHB310 (VHB310Q) /CSM/VH/VHB3-A/VHB310Q.Seq.d/                       1483   0.0
VHB194 (VHB194Q) /CSM/VH/VHB1-D/VHB194Q.Seq.d/                       1483   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.  1328   0.0    3
AR550596|AR550596.1 Sequence 5727 from patent US 6747137.          78   8e-46  6
AF107286|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds.    78   4e-44  6
AY497659|AY497659.1 Candida dubliniensis strain CBS 7987 translation elongation factor gene, partial cds.    78   6e-44  5
EH015431|EH015431.1 USDA-FP_188032 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt081_G08 5', mRNA sequence.    78   6e-44  4
EH012247|EH012247.1 USDA-FP_185167 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt044_H07 5', mRNA sequence.    78   9e-44  4
CZ289188|CZ289188.1 cp62f05.f Candida parapsilosis Random Genomic Library Candida parapsilosis genomic clone cp62f05, genomic survey sequence.    72   1e-43  7
Y09664|Y09664.1 C.albicans EFT2 gene.                              78   2e-43  6
CZ282959|CZ282959.1 cp27c04.f Candida parapsilosis Random Genomic Library Candida parapsilosis genomic clone cp27c04, genomic survey sequence.    72   3e-43  7
AF107289|AF107289.1 Candida tropicalis elongation factor 2 (EFT2) gene, partial cds.    70   7e-42  6

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   303   4e-81
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   301   1e-80
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   298   1e-79
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   297   3e-79
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   296   4e-79
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   296   4e-79
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   296   4e-79
(P05197) RecName: Full=Elongation factor 2;          Short=EF-2;...   296   4e-79
AJ278147_1(AJ278147|pid:none) Rattus norvegicus partial Ef-2 gen...   296   4e-79
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   296   4e-79

 psg: 0.58  gvh: 0.38  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 32.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHB310 is cyt

>VHB310Z.Seq
NNNNNNNNNNCTCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGCTGATAACC
ACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTG
CCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTT
TCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAG
GTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTG
GTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAA
CTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTA
TTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTT
CCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTG
ATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTT
CAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGA
GAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAA
TTTAAGTCGCACTTGAGTTTTCAAANATAGTAATCAAATCTAAAAAAAA