>VHB288 (VHB288Q) /CSM/VH/VHB2-D/VHB288Q.Seq.d/
XXXXXXXXXXTGGTGAACCATTGACTTCGCCATCAATCGTTAAAGCTTGCAAATTCCTTG
CCTCAAAACAAAGAGCAGATGGTGGTTGGGGCGAATCTTTCAAATCAAATGTTACCAAAG
AATACGTTCAACATGAAACTTCACAAGTTGTAAACACTGGTTGGGCTTTACTCTCACTCA
TGTCTGCTAAATATCCAGATAGAGAATGCATTGAAAGAGGTATTAAATTCCTCATACAAA
GACAATATCCAAATGGTGATTTCCCACAAGAATCTATTATTGGTGTTTTCAATTTCAATT
GTATGATCAGTTATTCAAATTATAAAAATATTTTCCCATTATGGGCTTTATCTAGATATA
ATCAATTATATTTAAAATCTAAAATTTAAATATTTCTAAAAAAAACAAATATATTAAAAA
AATTCTATTAATAATAATAATAATTTTAAAAAATATAATAATAACAATAATAGTTTAAAA
ATAATAAATAATAATAATAATAATAATAATAATAGTAAAACATAAATATAAATAATAATA
GTAATATGAATAGCAATAATAATAATAATAATTATAATAATAATAATAATAAGTCGATTA
TAAAATAATAATAATAGTAATAATAATAGTAATAATAATAGTAATAATAATTTTTAGGTA
AAAGTTTGTTCATTTATATGGTGTTTAATAAAACATTATATCGACAGTGCAACTAATTTT
TTTTATTATTGTTATTATTACTATTTTTATTGTTAATTATTTCTTATATGTAATTCAATA
AACTCATTATCATTT

---GEPLTSPSIVKACKFLASKQRADGGWGESFKSNVTKEYVQHETSQVVNTGWALLSLM
SAKYPDRECIERGIKFLIQRQYPNGDFPQESIIGVFNFNCMISYSNYKNIFPLWALSRYN
QLYLKSKI*iflkktnilkkfy*****f*ki***q**fknnk**********nininnns
nmnsnnnnnnynnnnnksiik***************flgkslfiymvfnktlyrqcn*ff
lllllllfllliisym*fnkliii

Frame A:
---GEPLTSPSIVKACKFLASKQRADGGWGESFKSNVTKEYVQHETSQVVNTGWALLSLM
SAKYPDRECIERGIKFLIQRQYPNGDFPQESIIGVFNFNCMISYSNYKNIFPLWALSRYN
QLYLKSKI*iflkktnilkkfy*****f*ki***q**fknnk**********nininnns
nmnsnnnnnnynnnnnksiik***************flgkslfiymvfnktlyrqcn*ff
lllllllfllliisym*fnkliii


Frame B:
---vnh*lrhqslklanslpqnkeqmvvganlsnqmlpkntfnmklhkl*tlvglyshsc
llniqienalkevlnssykdniqmvishknlllvfsisiv*sviqiikifshyglyldii
nyi*nlkfkyf*kkqiy*knsinnnnnfkkynnnnnslkiinnnnnnnnnskt*i*iiiv
i*iaiiiiiiiiiiiisrl*nnnnsnnnsnnnsnnnf*vkvcsfiwclikhyidsatnff
yycyyyyfyc*lflicnsinslsf


Frame C:
---*tidfainr*slqipclktksrwwlgrifqikcyqrirst*nftsckhwlgftlthv
c*isr*rmh*kry*iphtktiskw*fptriyywcfqfqlydqlfkl*kyfpimgfi*i*s
iifki*nlniskknkyikkilliiiiilkniiitiiv*k**iiiiiiiiivkhkyk****
ye*q*****l******vdykiiiiviiiviiiviiifr*kfvhlygv**niistvqliff
iiviitifivnyflyviq*thyh

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSH749 (VSH749Q) /CSM/VS/VSH7-C/VSH749Q.Seq.d/                        743   0.0
VHQ353 (VHQ353Q) /CSM/VH/VHQ3-C/VHQ353Q.Seq.d/                        743   0.0
VHQ272 (VHQ272Q) /CSM/VH/VHQ2-C/VHQ272Q.Seq.d/                        743   0.0
VHN722 (VHN722Q) /CSM/VH/VHN7-A/VHN722Q.Seq.d/                        743   0.0
VHN471 (VHN471Q) /CSM/VH/VHN4-C/VHN471Q.Seq.d/                        743   0.0
VHM690 (VHM690Q) /CSM/VH/VHM6-D/VHM690Q.Seq.d/                        743   0.0
VHM536 (VHM536Q) /CSM/VH/VHM5-B/VHM536Q.Seq.d/                        743   0.0
VHL437 (VHL437Q) /CSM/VH/VHL4-B/VHL437Q.Seq.d/                        743   0.0
VHJ537 (VHJ537Q) /CSM/VH/VHJ5-B/VHJ537Q.Seq.d/                        743   0.0
VHJ385 (VHJ385Q) /CSM/VH/VHJ3-D/VHJ385Q.Seq.d/                        743   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF159949|AF159949.1 Dictyostelium discoideum cycloartenol synthase (CAS1) mRNA, partial cds.   686   0.0    2
EC757725|EC757725.1 PPE00000938 Agencourt Biosciences Agen-0020 Non-normalized library cloned into Not I - Eco RV sites of pAGEN-1 Physarum polycephalum cDNA, mRNA sequence.    58   2e-05  2
EJ193077|EJ193077.1 1092344215047 Global-Ocean-Sampling_GS-27-01-01-1P8-2P0KB marine metagenome genomic clone 1061001429043 5', genomic survey sequence.    34   1.6    3
EB115421|EB115421.1 000713AAGA999056HT (AAGA) Pinkie expanding leaf, normalised 0h +M13 Malus x domestica cDNA clone AAGA999056, mRNA sequence.    32   3.5    3
CO128034|CO128034.1 GR__Eb12O13.r GR__Eb Gossypium raimondii cDNA clone GR__Eb12O13 3', mRNA sequence.    44   5.9    1
AI539933|AI539933.1 SWOvAFCAP27G01SK Onchocerca volvulus adult female cDNA (SAW98MLW-OvAF) Onchocerca volvulus cDNA clone SWOvAFCAP27G01 5', mRNA sequence.    44   5.9    1
BQ881121|BQ881121.1 AGENCOURT_8228609 Lupski_dorsal_root_ganglion Homo sapiens cDNA clone IMAGE:6181900 5', mRNA sequence.    44   5.9    1
AC196627|AC196627.3 Dasypus novemcinctus clone VMRC5-135N5, WORKING DRAFT SEQUENCE, 2 ordered pieces.    44   5.9    1
AC194674|AC194674.1 Zea mays chromosome 2 clone CH201-489L12; ZMMBBc0489L12, *** SEQUENCING IN PROGRESS ***, 7 unordered pieces.    44   5.9    1
AC126994|AC126994.3 Rattus norvegicus clone CH230-295C20, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    44   5.9    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55D85) RecName: Full=Cycloartenol synthase;          EC=5.4.99...   261   2e-68
AK312489_1(AK312489|pid:none) Homo sapiens cDNA, FLJ92849, highl...   144   3e-33
BC035638_1(BC035638|pid:none) Homo sapiens lanosterol synthase (...   144   3e-33
AK296313_1(AK296313|pid:none) Homo sapiens cDNA FLJ58192 complet...   144   3e-33
AJ720861_1(AJ720861|pid:none) Gallus gallus mRNA for hypothetica...   143   6e-33
AJ239031_1(AJ239031|pid:none) Homo sapiens LSS gene, partial, ex...   143   7e-33
BC134214_1(BC134214|pid:none) Danio rerio lanosterol synthase (2...   142   9e-33
BX255930_10(BX255930|pid:none) Zebrafish DNA sequence from clone...   142   9e-33
GN121451_1(GN121451|pid:none) Sequence 4347 from Patent WO200903...   140   4e-32
(P48450) RecName: Full=Lanosterol synthase;          EC=5.4.99.7...   140   4e-32

 psg: 0.70  gvh: 0.45  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.30  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for VHB288 is cyt

>VHB288Z.Seq
NNNNNNNNNNTGGTGAACCATTGACTTCGCCATCAATCGTTAAAGCTTGCAAATTCCTTG
CCTCAAAACAAAGAGCAGATGGTGGTTGGGGCGAATCTTTCAAATCAAATGTTACCAAAG
AATACGTTCAACATGAAACTTCACAAGTTGTAAACACTGGTTGGGCTTTACTCTCACTCA
TGTCTGCTAAATATCCAGATAGAGAATGCATTGAAAGAGGTATTAAATTCCTCATACAAA
GACAATATCCAAATGGTGATTTCCCACAAGAATCTATTATTGGTGTTTTCAATTTCAATT
GTATGATCAGTTATTCAAATTATAAAAATATTTTCCCATTATGGGCTTTATCTAGATATA
ATCAATTATATTTAAAATCTAAAATTTAAATATTTCTAAAAAAAACAAATATATTAAAAA
AATTCTATTAATAATAATAATAATTTTAAAAAATATAATAATAACAATAATAGTTTAAAA
ATAATAAATAATAATAATAATAATAATAATAATAGTAAAACATAAATATAAATAATAATA
GTAATATGAATAGCAATAATAATAATAATAATTATAATAATAATAATAATAAGTCGATTA
TAAAATAATAATAATAGTAATAATAATAGTAATAATAATAGTAATAATAATTTTTAGGTA
AAAGTTTGTTCATTTATATGGTGTTTAATAAAACATTATATCGACAGTGCAACTAATTTT
TTTTATTATTGTTATTATTACTATTTTTATTGTTAATTATTTCTTATATGTAATTCAATA
AACTCATTATCATTT