>VHB286 (VHB286Q) /CSM/VH/VHB2-D/VHB286Q.Seq.d/
XXXXXXXXXXTGGTTCAACCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCCTAC
ATTCAAGATCCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGT
GCATCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGAT
GGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTG
ATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTA
TCACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCT
CAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAG
ATTCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAAT
ATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCA
ACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAA
AAATTATCAAATTTATCAAGATTAAAATGGAAAACCGTT

---VQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDG
RGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQ
YQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLST
IFSTITITSATEMNLTGVQKLSNLSRLKWKTV

Frame A:
---gstnqw*lctcecyclhsrs*siitihhch**kswmcivkrwsigyddassyfer*w
swcwstne*vnsnryn**idfp*yfklcsislsscctlfitsitpnvynhstifkrlefs
isrclltidfsltftkwfkdsnstmvr*sr*yhtfty*kylsn*wsrftrstnyhfrsfn
ylfnnynhfcn*nefnwcskiikfikikmenr


Frame B:
---VQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDG
RGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQ
YQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLST
IFSTITITSATEMNLTGVQKLSNLSRLKWKTV


Frame C:
---fnqpvvimyl*mllptfkilinhynsplslievvdvhr*emvnwi**civvl*kmmv
vvlvnq*msqlkslqlvn*fsmifqvmlnliivllhslyhihysqclqplnnlqtigiln
ikvfthh*lqphlyqmv*rfklyng*iikiipyfyvlkifiklmvkihkihklsl*ifql
sfqqlqsllqlk*i*lvfknyqiyqd*ngkp

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB286 (VHB286Q) /CSM/VH/VHB2-D/VHB286Q.Seq.d/                       1247   0.0
VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/                       1239   0.0
VHP823 (VHP823Q) /CSM/VH/VHP8-A/VHP823Q.Seq.d/                       1239   0.0
VHP677 (VHP677Q) /CSM/VH/VHP6-D/VHP677Q.Seq.d/                       1239   0.0
VHP443 (VHP443Q) /CSM/VH/VHP4-B/VHP443Q.Seq.d/                       1239   0.0
VHP436 (VHP436Q) /CSM/VH/VHP4-B/VHP436Q.Seq.d/                       1239   0.0
VHP293 (VHP293Q) /CSM/VH/VHP2-D/VHP293Q.Seq.d/                       1239   0.0
VHO880 (VHO880Q) /CSM/VH/VHO8-D/VHO880Q.Seq.d/                       1239   0.0
VHO790 (VHO790Q) /CSM/VH/VHO7-D/VHO790Q.Seq.d/                       1239   0.0
VHO689 (VHO689Q) /CSM/VH/VHO6-D/VHO689Q.Seq.d/                       1239   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1215   0.0    1
AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.    32   0.066  8
AP007341|AP007341.1 Lotus japonicus genomic DNA, chromosome 2, clone:LjT35G04, TM0425.    40   0.098  2
EG619833|EG619833.1 EMBRYOF088227O8 POSSUM_01-POSSUM-C-EMBRYO-2KB Trichosurus vulpecula cDNA clone 1061028989863, mRNA sequence.    46   1.2    1
AM025232|AM025232.1 Bos indicus 3' EST, clone C0005915l21.         34   3.1    2
BH703301|BH703301.1 BOMNG55TR BO_2_3_KB Brassica oleracea genomic clone BOMNG55, DNA sequence.    40   3.1    2
AA382162|AA382162.1 EST95325 Activated T-cells II Homo sapiens cDNA 5' end, mRNA sequence.    40   3.7    2
EH243429|EH243429.1 JGI_ACAC13551.fwd ACAC Phakopsora pachyrhizi TW72-1 germinating urediniospores on water surface (20C/16 hours/dark) Phakopsora pachyrhizi cDNA clone ACAC13551 5', mRNA sequence.    44   4.7    1
AV262947|AV262947.1 Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone:4930432P20, 3' end partial sequence.    44   4.7    1
EH220383|EH220383.1 JGI_ABWZ1277.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA clone ABWZ1277 3', mRNA sequence.    44   4.7    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   350   2e-95
(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   350   2e-95
BT036325_1(BT036325|pid:none) Zea mays full-length cDNA clone ZM...    92   2e-17
AK068415_1(AK068415|pid:none) Oryza sativa Japonica Group cDNA c...    91   4e-17
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...    88   2e-16
EF146940_1(EF146940|pid:none) Populus trichocarpa clone WS01225_...    87   4e-16
AC148217_12(AC148217|pid:none) Medicago truncatula clone mth2-20...    86   9e-16
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...    85   2e-15
AB005230_20(AB005230|pid:none) Arabidopsis thaliana genomic DNA,...    85   2e-15
AB026648_12(AB026648|pid:none) Arabidopsis thaliana genomic DNA,...    84   3e-15

 psg: 0.82  gvh: 0.45  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VHB286 is cyt

>VHB286Z.Seq
NNNNNNNNNNTGGTTCAACCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCCTAC
ATTCAAGATCCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGT
GCATCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGAT
GGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTG
ATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTA
TCACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCT
CAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAG
ATTCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAAT
ATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCA
ACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAA
AAATTATCAAATTTATCAAGATTAAAATGGAAAACCGTT